The Activity of Matrix Metalloproteinase-2 and -9 in Serum of Pregnant and Non-Pregnant Bitches     

S Schäfer-Somi  O Ali Aksoy  M Patzl  M Findik  N Erünal-Maral  HB Beceriklisoy  B Polat  S Aslan 《Reproduction in domestic animals》2005,40(1):46-50

The aim of this study was to investigate, whether the activity of matrix metalloproteinase (MMP)-2 and -9 in the serum of pregnant and non-pregnant bitches differs significantly. For this purpose, 81 blood samples were taken from pregnant bitches at days 5-13, 15-21, 24-31, 34-40 and 41-50 after mating, and 51 samples from non-pregnant animals at corresponding times. Relative enzyme activity, calculated as the percentage of serum enzyme activity on enzyme activity in a control sample, was determined with a commercially available assay after activation of serum MMPs with p-aminophenylmercuric acetate (APMA). In addition, serum oestradiol (E(2)) and progesterone (P(4)) concentrations were measured with an enzymeimmunoassay (EIA). In the pregnant bitches, at days 5-13 and 15-21 after mating, the mean activity of both MMPs was significantly higher than in non-pregnant animals (28.5% vs 24.5% and 27.7% vs 22.6%; p < 0.01). Moreover, in the pregnant bitches, significant correlations were detected between the serum enzyme activity and the serum concentrations of E(2) (-0.900; p < 0.05) and P(4) (+0.667; p < 0.05).  相似文献   

A Screening for the Occurrence of Autoreactive Antibodies in Sera of Pregnant and Non-pregnant Bitches     

S Schäfer-Somi  KR Herkner  S Neubauer  M Egerbacher  M Patzl  S Pollak  O Ali Aksoy  HB Beceriklisoy  H Kanca  M Findik  H Kalender  S Aslan 《Reproduction in domestic animals》2006,41(1):48-54

Sera of healthy pregnant (group I, n = 11) and non-pregnant (group II, n = 11) bitches were screened for autoantibodies (AAb). In both groups, blood samples were drawn every fifth day between days 5 and 55 after mating. Serum was analysed via indirect immunofluorescence (IIF) with the Canine ANA HEp-2 Screening Kit. In all animals, anticytoplasmic AAb were detected. Utilizing primate-heart substrate slides AAb against contractile proteins of the cytoplasm could be observed. The predominating fluorescence pattern in pregnant animals resembled above all desmin, which was proven via Western blot. The sera were then pre-incubated with tropomyosin, actin, vimentin, vinculin and keratin solutions, and assessed on HEp-2 slides and on human and canine fibroblasts as well. The latter substrate was used to verify whether the detected Ab were in fact AAb. Utilizing tropomyosin, revealed elimination of the cytoplasmic fluorescences on all three substrates. It is therefore assumed, that in sera of healthy dogs, AAb against contractile structure proteins of the cytoplasm are present regularly. The majority of pregnant bitches presented with higher end-point titres (EPT), than to be found in non-pregnant dogs. AAb against desmin played the key role in those patterns. In addition, sera were screened for thyroid specific AAb, namely thyroglobulin, thyroid peroxidase (TPO), T3 and T4, and for AAb against insulin by ELISA or Western blot (TPO). Only in two of the pregnant bitches a weak positive reaction (1:100) for T3-AAb was detected.  相似文献   

Cytoprotective effect of piperine against benzo[a]pyrene induced lung cancer with reference to lipid peroxidation and antioxidant system in Swiss albino mice   总被引：2，自引：0，他引：2  

Selvendiran K  Singh JP  Krishnan KB  Sakthisekaran D 《Fitoterapia》2003,74(1-2):109-115

The cytoprotective effect of piperine on benzo[a]pyrene (B[a]P) induced experimental lung cancer was investigated in male Swiss albino mice. Oral administration of piperine (100 mg/kg body wt.) effectively suppressed lung cancer initiated with B[a]P as revealed by the decrease in the extent of lipid peroxidation with concomitant increase in the activities of enzymatic antioxidants (superoxide dismutase, catalase and glutathione peroxidase) and non-enzymatic antioxidant (reduced glutathione, vitamin E and vitamin C) levels when compared to lung cancer bearing animals. Our data suggest that piperine may extend its chemopreventive effect by modulating lipid peroxidation and augmenting antioxidant defense system.  相似文献   

Comparison of results from the semiautomated serum bone alkaline phosphatase isoenzyme assay with the periosteal alkaline phosphatase assay for use in rat models     

Powers CS  Schultze AE  Krishnan V  Sato M  Hoffmann WE 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2007,36(3):285-287

BACKGROUND: Assessment of bone formation activity is an important component of pharmacologic efficacy and toxicity evaluations for compounds in development for osteoporosis therapies. Antemortem biomarkers of bone formation and remodeling in rodents are uncommon. While the periosteal alkaline phosphatase (ALP) assay is a postmortem and laborious means of testing bone-building activity, the semiautomated ALP isoenzyme assay is an antemortem assay that is performed on an automated chemistry analyzer after 2 simple dilutions of the initial serum sample and a short incubation. OBJECTIVES: The goal of our investigation was to determine if the serum bone ALP (BALP) data obtained from the semiautomated ALP isoenzyme assay had a similar pattern of response when compared with the periosteal ALP (PALP) assay for use in pharmacologic screening in rats. METHODS: Serum and bone tissue samples were obtained from orchidectomized Wistar rats, a model of clinically induced osteoporosis. Subsequent bone formation was initiated via treatment with one of several compounds. In study 1, orchidectomized male rats were given either vehicle, dihydrotestosterone or a testosterone derivative subcutaneously every 4 days for 28 days. In study 2, orchidectomized male rats were given either vehicle or compounds A, B, or C by oral gavage daily for 15 days. Blood and tibias were collected at necropsy. Serum was analyzed for BALP activity using a semiautomated ALP assay. Tibias from the same rats were analyzed for PALP activity. RESULTS: Serum BALP activity paralleled PALP activity within each group when compared with the controls. CONCLUSION: Our data indicate that the semiautomated serum BALP isoenzyme assay may be used as a biomarker of bone-building potential in rat models of osteoporosis. This assay affords many advantages to investigators of musculoskeletal diseases, including the potential to measure multiple data points in a single study.  相似文献   

Imbibition of soybean seeds in warm water results in the release of copious amounts of Bowman-Birk protease inhibitor, a putative anticarcinogenic agent     

Palavalli MH  Natarajan SS  Wang TT  Krishnan HB 《Journal of agricultural and food chemistry》2012,60(12):3135-3143

Protease inhibitors play a protective role against pathogenic microorganisms and herbivorous insects. The two predominant protease inhibitors of soybean seeds are the Kunitz trypsin inhibitor (KTI) and Bowman-Birk protease inhibitor (BBI). In this study, we report that soybean seeds incubated in warm water release large amounts of proteins into the surrounding media. Two-dimensional gel electrophoresis analysis of the seed exudates resulted in the separation of 93 distinct protein spots out of which 90 spots were identified by LC-MS/MS. The basic 7S globulin and the BBI are the two predominant proteins found in the soybean seed exudates. In addition to 7S and 11S seed storage proteins, others known to protect the seeds against pathogens and pests including KTI, peroxidase, α-galactosidase, and endo-1.3-β-glucanase were also identified in the seed exudates. Soybean seed exudate obtained by incubating the seeds in warm water was also able to inhibit the growth of human breast cancer cell line MCF-7. Since soybean seeds release large amounts of enzymatically active BBI when immersed in warm water, our procedure could be exploited as a simplified alternative method for the preparation of BBI concentrate which is being used as a cancer chemoprotective agent.  相似文献   

African Potato Association     

Eugene Marshack  K. S. Krishnan  Teruzo Asahara  Eduardo Vioque  Reinhard Marcuse 《Potato Research》1988,31(4):579-579

News

African Potato Association  相似文献   

Immuno-histological mapping and functional association of seminal proteins in testis and excurrent ducts with sperm function in buffalo     

Sunil Kumar Patil  Lakshminarayana Somashekar  Sellappan Selvaraju  Kolatalu V. Jamuna  Shivashanmugam Parthipan  Bala Krishnan Binsila  Ramaswamy Venkatesha Prasad  Janivara Parameshwaraiah Ravindra 《Reproduction in domestic animals》2020,55(8):998-1010

The region-specific expression of seminal proteins in testis and excurrent duct system determines the quality and function of the spermatozoa. In the present study, localization and expression of some of the seminal proteins such as insulin-like growth factor receptor 1β (IGF-1Rβ), phosphatidylethanolamine-binding protein 4 (PEBP4), α-tubulin and tissue factor pathway inhibitor 2 (TFPI2) were carried out in testis, excurrent duct system and spermatozoa of buffalo. IGF-1Rβ was localized in the cells of the seminiferous tubules of the testis, except in primary spermatocytes. The PEBP4 was localized only in the elongated spermatid, whereas α-tubulin and TFPI2 proteins were localized in all cells of the seminiferous tubule including spermatocyte. In the buffalo spermatozoa, IGF-1Rβ, PEBP4, α-tubulin and TFPI2 were localized in the acrosome region, the post-acrosomal region till the tail end, post-acrosome to the entire tail region and the equatorial region, respectively. The study indicates that IGF-1R, α-tubulin and PEBP4 proteins regulate spermatogenesis, whereas TFPI2 may be involved during the zona binding process of the buffalo spermatozoa.  相似文献   

A Crop–Weather Model for Prediction of Rice (Oryza sativa L.) Yield Using an Empirical-Statistical Technique     

K. Kandiannan  R. Karthikeyan  R. Krishnan  C. Kailasam  & T. N. Balasubramanian 《Journal of Agronomy and Crop Science》2002,188(1):59-62

Rice is the staple food in many countries and is grown in varied climates from per‐humid to semiarid areas. Crop–weather models were used to predict rice yield in India. However, in spite of a significant influence of solar radiation on rice yield, none of these models used solar radiation as one of the predictors. In this paper, an attempt was made to predict the first season (June–September) rice yield at Coimbatore, Tamil Nadu, India by including solar radiation as one of the predictors. Ten years (1987/88–1996/97) data were used for the study. Seven predictors viz., percentage of rice area during first season (X₁), number of days with minimum temperature below 22 °C in August and September (X₂), average daily maximum temperature for three months (July, August and September; X₃), average daily minimum temperature for three months (July, August and September; X₄), total of average sunshine hours in August and September (X₅), and total rainfall of July, August and September (X₆) total average solar radiation of August and September (X₇) were selected based on earlier report. Full model and stepwise regression analysis were performed using MSTAT computer package. The full model regression without solar radiation as predictor (Model I) recorded comparatively less R² (0.6292). Inclusion of solar radiation (Model II) enhanced the R² value considerably (R²=0.9464). Seven variables were further subjected to stepwise regression analysis and only four predictors were retained in the final model (Model III) with an R² value of 0.9234. The model III with minimum parameters Y=22119.5758 + 19.6898, X₁ ? 150.9261, X₂ ? 1126.7501, X₄ + 0.7179 X7 can be used to predict the first season rice yield (Y) at Coimbatore, India.  相似文献   

Introgression of multiple disease resistance into a maintainer of Basmati rice CMS line by marker assisted backcross breeding     

Ashok K. Singh  Vikas K. Singh  Atul Singh  Ranjith K. Ellur  R. T. P. Pandian  S. Gopala Krishnan  U. D. Singh  M. Nagarajan  K. K. Vinod  K. V. Prabhu 《Euphytica》2015,201(1):97-107

  相似文献   

Nitrogen lowers the sulfur amino acid content of soybean (Glycine max [L.] Merr.) by regulating the accumulation of Bowman-Birk protease inhibitor     

Krishnan HB  Bennett JO  Kim WS  Krishnan AH  Mawhinney TP 《Journal of agricultural and food chemistry》2005,53(16):6347-6354

Soybeans in general contain 35-40% protein. Efforts are underway to increase further this protein content, thus enhancing their nutritive value. Even though higher protein is a desirable characteristic, whether such an increase will be accompanied by enhanced protein quality is not known. Soybean protein quality could be significantly improved by increasing the concentration of the sulfur-containing amino acids, cysteine and methionine. To ascertain if a correlation existed between protein quantity and quality, a comparison of the amino acids of soybeans differing in protein content was made. Soybeans with higher protein content had a significantly lower percentage of sulfur amino acids, while those with lower protein exhibited a higher content of cysteine and methionine. Nitrogen application elevated the protein content but lowered that of the sulfur amino acids. Transmission electron microscopy examination of thin sections of low protein soybean seeds revealed several protein storage vacuoles that were partially filled with storage proteins. Fluorescence two-dimensional difference gel electrophoresis of soybean seed proteins revealed that nitrogen application favored the accumulation of the beta-subunit of beta-conglycinin while decreasing the accumulation of Bowman-Birk protease inhibitor (BBI), a protein rich in cysteine. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of 60% 2-propanol-extracted proteins showed a drastic reduction in the accumulation of BBI with increasing protein content. Northern blot analysis indicated that nitrogen had a negative influence on the expression of the BBI gene. Our results indicate that the negative correlation between total protein and sulfur amino acid content is mostly mediated by the differential accumulation of BBI.  相似文献