Effect of stunning voltage and scalding method on the incidence of the “red skin” defect of Parma ham     

D. P. Lo Fiego  M. Comellini  M. C. Ielo  F. Tassone  L. A. Volpelli 《Veterinary research communications》2009,33(Z1):285-288

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Effects of dexamethasone on peripheral blood mononuclear cell phenotype in weanling piglets     

Lo DY  Lee WM  Chien MS  Lin CC  Lee WC 《Comparative immunology, microbiology and infectious diseases》2005,28(4):251-258

The aim of this study was to evaluate the effect of dexamethasone treatment on the immune system of weanling piglets. Piglets were administered dexamethasone (DEX; 1mg/kg, IM) every 12h for 2 consecutive days (short-term experiment) or DEX (1mg/kg, IM) daily for 2 weeks (long-term experiment). The relative percentage of CD8(+) T cells in peripheral blood mononuclear cells (PBMCs) was significantly decreased (P<0.05) in both short- and long-term DEX-treated groups compared to their control groups. The percentage of IgM(+) cells in PBMCs of the long-term DEX-treated group was greatly increased (P<0.05) in comparison to the control group. The results of this study indicate that short-term DEX-treatment increases leucocyte function; however, long-term DEX-treatment depresses leucocyte function, especially that of CD8(+) T cells.  相似文献   

Effect of resting time of pigs and pre-chilling time of thighs on the veining defect of parma dry-cured ham     

Costa LN  Lo Fiego DP  Tassone F  Russo V 《Veterinary research communications》2005,29(Z2):375-377

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Construction and analysis of a Mannheimia haemolytica A1 luxS mutant     

van der Vinne AN  Lo RY  Shewen PE 《Veterinary microbiology》2005,110(1-2):53-66

Mannheimia haemolytica A1 is the causative agent of bovine pneumonic pasteurellosis, a major cause of sickness, death, and economic loss to the feedlot cattle industry. M. haemolytica A1 produces autoinducer-2 (AI-2) like molecules that are capable of inducing quorum sensing system 2 of Vibrio harveyi. This interspecies quorum sensing system has been shown to regulate the expression of virulence genes in several pathogenic bacteria. The protein central to the production of AI-2 is LuxS. To determine if quorum sensing is involved in the regulation of virulence genes in M. haemolytica A1, a luxS mutant was constructed by replacing luxS with a cat cassette. This mutant was verified by PCR analysis, Southern hybridization, as well as its inability to induce bioluminescence in the V. harveyi reporter strain. RT-PCR analysis showed there was no difference in leukotoxin (lktC) mRNA levels, however there were increased mRNA levels of putative virulence associated genes, transferrin binding protein B (tbpB), adhesin (ahs) and capsule biosynthesis (nmaA). Electron microscopy showed that the level of encapsulation in the mutant is higher than the parent. Additionally, the mutant was slightly more adherent to bovine tracheal cells than the parent. In vitro competition assays showed the mutant out-competed the parent under iron-restricted conditions. However, in a calf challenge, the parent was the dominant isolate recovered.  相似文献   

Principles, application areas and an example of risk assessment conducted at the Danish Institute for Food and Veterinary Research     

Greiner M  Paisley L  Hostrup-Pedersen J  Lo Fo Wong DM  Andersen JS  Stockmarr A  Korsgård H  Sommer HM  Hald T 《Berliner und Münchener tier?rztliche Wochenschrift》2004,117(5-6):177-181

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Development of a predictive equation for the subcutaneous fat thickness of pig thighs     

Lo Fiego DP  Falcone PM  Ielo MC 《Veterinary research communications》2008,32(Z1):S347-S349

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Cross-scale effects of land use on the functional composition of herbivorous insect communities     

Neff  Felix  Blüthgen  Nico  Chisté  Melanie N.  Simons  Nadja K.  Steckel  Juliane  Weisser  Wolfgang W.  Westphal  Catrin  Pellissier  Loïc  Gossner  Martin M. 《Landscape Ecology》2019,34(8):2001-2015

Landscape Ecology - Insect herbivores comprise the majority of macroinvertebrate communities of temperate grasslands and act as drivers for important ecosystem functions. Landscape- and local-level...  相似文献   

Determination of the antibiotic fungicide validamycin A in formulated products by micellar electrokinetic chromatography.   总被引：1，自引：0，他引：1  

Y M Hsiao  C C Lo 《Journal of agricultural and food chemistry》1999,47(9):3723-3726

A micellar electrokinetic capillary chromatographic method (MEKC) was used to determine validamycin A content in commercial products. The results indicated that this method was capable of analyzing the validamycin A content in formulated products with an instrument detection limit of 0.94 microg/mL and a method detection limit of 1. 70 microg/mL. Relative standard deviation (RSD) values of MEKC determination of validamycin A in formulated products ranged from 0. 61 to 2.09%. Recoveries of validamycin A in formulated products were in the region of 99.5-105.1%. All commercial products collected from markets contained validamycin A. The high percentage of recovery, the low detection limit, and the low RSD values confirmed that the MEKC technique is a senstivie and selective method.  相似文献   

Genetic variation and its relationship to root weight in the sweet potato as revealed by RAPD analysis     

K.H. Lin  Y.C. Lai  H.C. Li  S.F. Lo  L.F.O. Chen  H.F. Lo 《Scientia Horticulturae》2009

The aim of this work was to evaluate the genetic variations of the sweet potato by a random amplified polymorphic DNA (RAPD) assay with emphasis on correlations with morphological traits. Cuttings of superior and inferior lines derived from tissue culture-regenerated plants and asexually propagated plants of the Tainung 57 (TN57) and Tainung 66 (TN66) sweet potato were planted in the field. Three important agronomic traits, top weight, root weight, and root numbers, were measured 3 months after planting. The RAPD-PCR (polymerase chain reaction) technique was used for detecting genomic variations within and between varieties. Of the 160 primers tested in this study, 38 revealed clear and repeatable RAPD polymorphisms. Among the 38 primers, 8 showed consistent amplified band patterns among the plants with variations within and between varieties, while the others indicated polymorphisms within or between varieties. RAPD markers demonstrated a clear association with root weight. However, the presence and absence of these genetic markers did not correspond well with either the top weight or root number. Genetic instability revealed in the selection stress of superior and inferior plants was found to be independent of variety. In addition, both asexual propagated and tissue culture-induced somaclonal variations were observed in this study. Our results show that RAPD is a useful tool for detecting somaclonal variations from varietal and intra-varietal sweet potato for monitoring of DNA changes in somaclonal variants. The identification of regions which are associated with the character of root weight by RAPD markers enables us to use these markers as selection tools to improve root weight in sweet potato.  相似文献   

Evolutionary impact assessment: accounting for evolutionary consequences of fishing in an ecosystem approach to fisheries management     

Ane T Laugen  Georg H Engelhard  Rebecca Whitlock  Robert Arlinghaus  Dorothy J Dankel  Erin S Dunlop  Anne M Eikeset  Katja Enberg  Christian Jørgensen  Shuichi Matsumura  Sébastien Nusslé  Davnah Urbach  Loїc Baulier  David S Boukal  Bruno Ernande  Fiona D Johnston  Fabian Mollet  Heidi Pardoe  Nina O Therkildsen  Silva Uusi‐Heikkilä  Anssi Vainikka  Mikko Heino  Adriaan D Rijnsdorp  Ulf Dieckmann 《Fish and Fisheries》2014,15(1):65-96

Managing fisheries resources to maintain healthy ecosystems is one of the main goals of the ecosystem approach to fisheries (EAF). While a number of international treaties call for the implementation of EAF, there are still gaps in the underlying methodology. One aspect that has received substantial scientific attention recently is fisheries‐induced evolution (FIE). Increasing evidence indicates that intensive fishing has the potential to exert strong directional selection on life‐history traits, behaviour, physiology, and morphology of exploited fish. Of particular concern is that reversing evolutionary responses to fishing can be much more difficult than reversing demographic or phenotypically plastic responses. Furthermore, like climate change, multiple agents cause FIE, with effects accumulating over time. Consequently, FIE may alter the utility derived from fish stocks, which in turn can modify the monetary value living aquatic resources provide to society. Quantifying and predicting the evolutionary effects of fishing is therefore important for both ecological and economic reasons. An important reason this is not happening is the lack of an appropriate assessment framework. We therefore describe the evolutionary impact assessment (EvoIA) as a structured approach for assessing the evolutionary consequences of fishing and evaluating the predicted evolutionary outcomes of alternative management options. EvoIA can contribute to EAF by clarifying how evolution may alter stock properties and ecological relations, support the precautionary approach to fisheries management by addressing a previously overlooked source of uncertainty and risk, and thus contribute to sustainable fisheries.  相似文献