A high-resolution radiation hybrid map of the human genome draft sequence     

Olivier M  Aggarwal A  Allen J  Almendras AA  Bajorek ES  Beasley EM  Brady SD  Bushard JM  Bustos VI  Chu A  Chung TR  De Witte A  Denys ME  Dominguez R  Fang NY  Foster BD  Freudenberg RW  Hadley D  Hamilton LR  Jeffrey TJ  Kelly L  Lazzeroni L  Levy MR  Lewis SC  Liu X  Lopez FJ  Louie B  Marquis JP  Martinez RA  Matsuura MK  Misherghi NS  Norton JA  Olshen A  Perkins SM  Perou AJ  Piercy C  Piercy M  Qin F  Reif T  Sheppard K  Shokoohi V  Smick GA  Sun WL  Stewart EA  Fernando J  Tejeda  Tran NM  Trejo T  Vo NT  Yan SC  Zierten DL  Zhao S 《Science (New York, N.Y.)》2001,291(5507):1298-1302

We have constructed a physical map of the human genome by using a panel of 90 whole-genome radiation hybrids (the TNG panel) in conjunction with 40,322 sequence-tagged sites (STSs) derived from random genomic sequences as well as expressed sequences. Of 36,678 STSs on the TNG radiation hybrid map, only 3604 (9.8%) were absent from the unassembled draft sequence of the human genome. Of 20,030 STSs ordered on the TNG map as well as the assembled human genome draft sequence and the Celera assembled human genome sequence, 36% of the STSs had a discrepant order between the working draft sequence and the Celera sequence. The TNG map order was identical to one of the two sequence orders in 60% of these discrepant cases.  相似文献   

培养条件对嗜水气单胞菌胞外蛋白酶合成与分泌的影响   总被引：5，自引：0，他引：5  

储卫华  陆承平 《南京农业大学学报》2001,24(3):65-68

对体外培养嗜水气单胞菌的胞外蛋白酶 (ECPase)产生条件进行了优化。结果发现 ,其产量与碳源、氮源、培养温度、培养基初始pH值等有关。蔗糖能促进蛋白酶的产生 ,NH4 + 抑制蛋白酶的产生。其最佳产酶条件为 :pH 7 5的0 0 2mol·L-1KC1,0 0 6mol·L-1K2 HPO4 ,5g·L-1的蔗糖和 5g·L-1的胰蛋白胨水 ,2 8℃ ,15 0r·min-1摇床培养 6 5h。在此条件下酶活性可达 2 0 8U·mL-1。  相似文献   

大弹涂鱼血细胞发生的研究   总被引：14，自引：0，他引：14       下载免费PDF全文

李长玲  曹伏君  刘楚吾  师尚丽 《中国水产科学》2001,8(1):10-14

通过对大鲜涂鱼血液、肾脏、脾脏组织涂片的观察，发现其肾脏、脾脏内白细胞的发育大致经过了3个阶段，即原始阶段、幼稚阶段、成熟阶段。造血母细胞从造血器官释放入外周血中有一成熟过程。着重描述了大弹涂鱼的红细胞、嗜中性粒细胞、淋巴细胞和单核细胞发生过程中各阶段细胞的形态特征。  相似文献   

Effects of Dietary Digestible Protein and Energy Levels on Growth Performance,Feed Utilization,and Body Composition of Juvenile Permit,Trachinotus falcatus (Linnaeus, 1758)          下载免费PDF全文

Huy Quang Nguyen  Thiet Chi Chu  Thuy Thi Le Nguyen  Ivar Lund 《Journal of the World Aquaculture Society》2018,49(5):943-952

A 9‐wk study was conducted to evaluate the effect of dietary protein and energy on growth performance of juvenile permit, Trachinotus falcatus, growing from approximately 30 to 150 g. Nine experimental diets were formulated to contain three levels of crude protein (400, 450, and 500 g/kg dry matter [DM]); and three levels of crude lipid (100, 200, and 300 g/kg DM) in a 3 × 3 factorial design. Growth rate and feed efficiency were significantly improved with increasing dietary protein levels from 400 to 500 g/kg and with dietary lipid levels from 100 to 200 g/kg. Fish body protein content was positively correlated with dietary ratio of digestible protein (DP) to digestible energy (DE) (P < 0.01, R² = 0.83), while body lipid was negatively correlated with dietary DP/DE (R² = 0.55, P < 0.05) but positively correlated with dietary DE levels (R² = 0.66, P < 0.01). Results showed a protein‐sparing effect, as protein retention was significantly increased by increasing dietary lipid level. In conclusion, the diet containing DP of 392.7 g/kg and DE of 18.8 MJ/kg (DM), corresponding to a DP/DE of 20.9 g/MJ, is suggested as an optimal feed for growth and feed efficiency in juvenile permit.  相似文献   

Apparent digestibility coefficients of selected protein feed ingredients for loach Misgurnus anguillicaudatus          下载免费PDF全文

Z.J. Chu  D.H. Yu  Y.C. Yuan  Y. Qiao  W.J. Cai  H. Shu  Y.C. Lin 《Aquaculture Nutrition》2015,21(4):425-432

Apparent digestibility coefficients of dry matter, crude protein, lipid and energy, and amino acids availability in white fish meal, brown fish meal, meat meal, fermented soybean meal, soybean meal and rapeseed meal were determined for loach (Misgurnus anguillicaudatus) (12.05 ± 0.21 g), using a reference diet with 5 g kg^?1 chromic oxide and test diets that contained 700 g kg^?1 reference diet, by weight, and 300 g kg^?1 of the test feed ingredients. The juvenile loach was held in 300‐l tanks at a density of 30 fish per tank. White fish meal, brown fish meal, meat meal and fermented soybean meal had highest apparent digestibility coefficients (ADCs) of dry matter, crude protein and gross energy among ingredients tested, ranged from 50.4% to 60.9% for dry matter, from 64.6% to 88.4% for crude protein and from 57.9% to 79.0% for gross energy. The apparent digestibility coefficients of dry matter ranged from 61.0% to 66.9% for animal products and 50.4% to 60.7% for plant products. For crude protein, apparent digestibility coefficients of protein exceeding 80% were observed for white fish meal, brown fish meal, meat meal and fermented soybean meal, and the apparent digestibility coefficients of protein in rapeseed meal were the lowest among all the treatments. Lipids from both animal and plant feedstuffs were poorly digested by loach, ranging from 64.0% to 77.6%. The apparent digestibility coefficients of energy were similar to those of dry matter and protein, and the highest and lowest ADCs of energy were found in WFM and RM, respectively. The loach used dietary phosphorus from the animal feedstuffs more efficiently than from plant feedstuffs (soybean meal and rapeseed meal), with ADC‐values ranging from 42.3% to 53.1% and from 25.1% to 32.7%, respectively. For the animal products, the availabilities of amino acids in white fish meal and brown fish meal were higher than that in meat meal, expect for Met, Asp, Pro, Gly, and Cys. Among all the plant products, the availabilities of amino acids in fermented soybean meal were higher than in soybean meal and rapeseed meal, and thus had a greater potential to be used as a dietary replacement of fish meal in loach diets.  相似文献   

潜流湿地技术在高密度养殖尾水处理中的应用     

朱晓荣  沈全华  褚秋芬  孟祥雨  王红卫  曹卢  宋学宏 《水产养殖》2012,33(10):17-23

构建"潜流湿地+生物净化"的湿地生态系统,并以纯生物净化系统作为对照区,研究其对池塘高密度养殖尾水中总氮(TN)、总磷(TP)、高锰酸盐指数(CODMn)、悬浮物总颗粒(SS)指标的去除效果,并对试验区与对照区内的产量与效益进行分析。结果表明:构建湿地生态系统后,区域内养殖面积有所减少,但产量与效益却有所提高。试验区的净化面积占整个养殖系统的7.9%,远远小于对照区的净化面积占比18.8%。对TN、TP、CODMn的去除率分别为80.8%～90.9%、78.95%～88.18%和23.20%～27.24%,对照区对相应指标的去除率分别为70.8%～88.7%、76.09%～87.14%和15.38%～25.08%,试验区的去除效率略高于对照区。至2011年,试验区与对照区的池塘生产各类水产品产量分别比2009年增长24.8%与19.2%、销售收入分别增长了26.4%与14.8%、利润分别增加了35.2%与18.64%。试验区产量与效益明显好于对照区。因此,潜流湿地方式净化水质节省了土地资源,提高了鱼池利用率及养殖效益,与纯生物净化相比具有明显的优越性。  相似文献   

“六五”至“十二五”期间我国棉花品种纤维品质评价分析   总被引：2，自引：0，他引：2  

唐淑荣  金石桥  付小琼  褚平  孟俊婷  侯爱玲  韦京艳  魏守军  杨伟华 《中国棉花》2015,42(9):18-24

对"六五"至"十二五"期间国家棉花区域试验参试品种纤维品质进行了总结和分析,结果表明,我国棉花品种的综合纤维品质总体趋势是稳步改良,逐年提升。黄河流域棉区品种的纤维长度和断裂比强度呈优化发展趋势;长江流域棉区品种纤维长度、断裂比强度和纺纱均匀性指数呈优化发展趋势;西北内陆棉区品种纤维品质总体表现有上升趋势,但近5年来纤维品质表现略有下降趋势。  相似文献   

SiO2纳米颗粒对杉木幼苗生长发育的影响     

刘晨  许业洲  杜超群  刘乐平  吴楚 《中南林业科技大学学报(自然科学版)》2020,(4):34-43

【目的】近年来,硅对于的植物影响受到人们广泛关注,人们试图将硅确定为植物生长发育的的“必要元素”。因此如何提高植物对硅的吸收利用来增加植物的农艺性状和产量,是当前农业和林业研究的一个重要课题。【方法】以黄枝杉Cunninghamia lanceolata和罗田垂枝杉C.lanceolata var.luotian为材料,采用盆栽的方式,研究施加不同浓度的纳米SiO2颗粒(0、1、2、4 g/盆)对杉木幼苗光合参数、叶绿素荧光、叶片厚度、氮磷钾含量及其分配、生物量累积的影响。【结果】本研究结果显示,SiO2纳米颗粒对杉木根系的生长发育有很大的促进作用,使之能更好的吸收养分,加强了氮磷钾含量的累积。且叶片的厚度也有所增加,在较低的浓度下,气孔导度、光合作用机制、以及光合作用效率增加;地上的生物量也随着施入SiO2纳米颗粒浓度的增加而增加。【结论】我们的实验表明,SiO2纳米颗粒通过促进根系发育加强对营养的吸收和提升叶片厚度,以及提高净光合速率和气孔导度来提高植物的生长机理,进而增加植物的生物量来提升产量。鉴于其对植物生长发育及产量的多种积极影响,硅纳米材料将会为农林植物增产有重要的影响和广阔的前景。  相似文献   

五味子的研究概况及其开发前景     

初广峻  黄淑华  孟伟利 《北京农业》2011,(24):25-26

通过对五味子的研究作简要概述,并试对我国五味子的资源培育、野生驯化、人工栽培、产业开发进行初步探讨。  相似文献   

新合院生活的景观体验——黄骅新城家园景观设计     

褚天骄  赵文斌  陆潇潇 《农业科技与信息》2010,(3)

文章从分析现状入手,依据建筑规划所形成的空间提出了新合院的景观设计理念,并通过对道路系统、出入口、水系、植物、老年儿童活动空间等景观空间的精心组织,形成小区一心、二街、三轴、四院的景观骨架,再结合对景、框景等造园手法来打造一个简洁朴实又不失特色的小区景观。  相似文献