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31.
Radiation hybrid mapping: a somatic cell genetic method for constructing high-resolution maps of mammalian chromosomes 总被引:69,自引:0,他引:69
Radiation hybrid (RH) mapping, a somatic cell genetic technique, was developed as a general approach for constructing long-range maps of mammalian chromosomes. This statistical method depends on x-ray breakage of chromosomes to determine the distances between DNA markers, as well as their order on the chromosome. In addition, the method allows the relative likelihoods of alternative marker orders to be determined. The RH procedure was used to map 14 DNA probes from a region of human chromosome 21 spanning 20 megabase pairs. The map was confirmed by pulsed-field gel electrophoretic analysis. The results demonstrate the effectiveness of RH mapping for constructing high-resolution, contiguous maps of mammalian chromosomes. 相似文献
32.
Altered response to pneumococcal polysaccharide in offspring of immunologically paralyzed mice 总被引:3,自引:0,他引:3
The dose of pneumococcal polysaccharide type III required to induce immunologic paralysis in newborn offspring of immunologically paralyzed mice was one-tenth of the corresponding paralyzing dose for newborn offspring of normal mice. Similarly, immunization of the offsprinig of the paralyzed mice was accomplished with one-tenth the dose of polysaccharide necessary to immunize normal newborn mice. The altered susceptibility of newborn mice from paralyzed mothers to the induction of both paralysis and immunity was predicted from theories of antibody formation which postulate that the induction of tolerance or immunity is controlled by the concentration of natural antibodies specific for the antigen used. 相似文献
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Abtahian F Guerriero A Sebzda E Lu MM Zhou R Mocsai A Myers EE Huang B Jackson DG Ferrari VA Tybulewicz V Lowell CA Lepore JJ Koretzky GA Kahn ML 《Science (New York, N.Y.)》2003,299(5604):247-251
Lymphatic vessels develop from specialized endothelial cells in preexisting blood vessels, but the molecular signals that regulate this separation are unknown. Here we identify a failure to separate emerging lymphatic vessels from blood vessels in mice lacking the hematopoietic signaling protein SLP-76 or Syk. Blood-lymphatic connections lead to embryonic hemorrhage and arteriovenous shunting. Expression of slp-76 could not be detected in endothelial cells, and blood-filled lymphatics also arose in wild-type mice reconstituted with SLP-76-deficient bone marrow. These studies reveal a hematopoietic signaling pathway required for separation of the two major vascular networks in mammals. 相似文献
36.
Myers EH 《Science (New York, N.Y.)》1933,77(2008):609-610
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Wohlmuth H Leach DN Smith MK Myers SP 《Journal of agricultural and food chemistry》2005,53(14):5772-5778
Ginger (Zingiber officinale Roscoe), a monocotyledonous, sterile cultigen, is widely used as a spice, flavoring agent, and herbal medicine. The pungency of fresh ginger is due to a series of homologous phenolic ketones of which [6]-gingerol is the major one. The gingerols are thermally unstable and can be converted to their corresponding shogaols, which are present in dried ginger. Fresh rhizomes of 17 clones of Australian ginger, including commercial cultivars and experimental tetraploid clones, were assayed by HPLC for gingerols and shogaols. [6]-Gingerol was identified as the major pungent phenolic compound in all samples, while [8]- and [10]-gingerol occurred in lower concentrations. One cultivar known as "Jamaican" contained the highest concentrations of all three gingerols and was the most pungent of the clones analyzed. Gingerols were stable in ethanolic solution over a 5-month period when stored at 4 degrees C. Shogaols were not identified in the extracts prepared from fresh rhizomes at ambient temperature, confirming that these compounds are not native constituents of fresh ginger. In contrast to previous findings, this study did not find significant differences in gingerol concentrations between the tetraploid clones and their parent diploid cultivar. 相似文献
39.
The selective toxicity of new N-phosphorothioyl-carbamate esters 总被引:4,自引:0,他引:4
M A Fahmy T R Fukuto R O Myers R B March 《Journal of agricultural and food chemistry》1970,18(5):793-796
40.
R. L. Grier G. Di Guardo R. Myers D. F. Merkley 《The Journal of small animal practice》1995,36(9):385-388
Distilled water was studied as an hypoosmotic oncolysis agent to prevent mast cell tumour (MCT) recurrence following surgical excision. The results from 118 MCTS excised in 74 dogs were: surgery alone, a recurrence rate of 52·6 per cent (10/19); and surgery followed by distilled water injections into the wound, a 26·2 per cent (26/99) recurrence rate. All of 10 small MCTS (0·03 to 0·25 cm3) injected with distilled water in situ with no surgery, regressed. The present data suggest that distilled water should be considered as an adjunct to surgery for canine MCTS. 相似文献