Potential host range of four Phytophthora interspecific hybrids from Clade 8a     

Fatemeh Jafari  Reza Mostowfizadeh-Ghalamfarsa  Banafsheh Safaiefarahani  Treena I. Burgess 《Plant pathology》2020,69(7):1281-1290

In recent years several interspecific hybrids have been reported in the plant pathogenic oomycete genus Phytophthora. Due to the large genotypic and phenotypic changes, these hybrids might have broader or more limited host ranges compared with their parental species. It is crucial to understand the host range of Phytophthora hybrids to minimize the economic losses caused by their infection. The potential host range of four hybrids belonging to Clade 8a of the Phytophthora phylogenetic tree was investigated in this study. Thirty species of herbaceous plants as well as eight species of woody plants were inoculated and monitored for any symptom of infection. In addition, the detached twigs of 32 tree species, fruits of six plant species, tubers of potato, and roots of carrot and sugar beet were investigated for susceptibility to these hybrids. Almost all hybrids caused severe rot on all tested fruits, tubers, and roots, although different isolates showed different pathogenicity on detached tree twigs. All hybrids tested had a different host range compared with their parental species: they were able to infect plants outside the host range of their parents, infect hosts of both parental species, although these parents did not have overlapping hosts, or, in some cases, they were not able to infect hosts infected by the parents.  相似文献   

Effects of emulsified versus saline administration of GnRHa on induction of ovulation in rainbow trout, Oncorhynchus mykiss     

Arya Vazirzadeh  Abdolmajid Hajimoradloo  Hamid Reza Esmaeili  Mostafa Akhlaghi 《Aquaculture (Amsterdam, Netherlands)》2008,280(1-4):267-269

The effects of saline-dissolved or Freund's incomplete adjuvant (FIA)-emulsified GnRHa treatment on the induction of ovulation in rainbow trout, Oncorhynchus mykiss, were examined. The FIA-emulsified GnRHa was first diluted in 0.25 ml physiological saline and then mixed with an equal volume of FIA. Fish were selected in the beginning of the spawning season and were allocated into four groups and were treated intraperitoneally with (a) 0.5 ml of emulsified GnRHa (GnRHa–FIA), (b) 0.5 ml saline-dissolved GnRHa in a single injection (GnRHa-1), (c) 0.5 ml saline-dissolved GnRHa in two injections spaced 1 d apart (GnRHa-2) and (d) 0.5 ml of saline (Control). The GnRHa dose in all hormone treatments was 25 µg kg^− 1. All fish in the FIA–GnRHa and GnRHa-2 groups ovulated within 10 and 11 d after treatment, respectively. In contrast, only 75% in the Control fish and 60% of the fish in the GnRHa-1 group ovulated within 36 d after treatment. None of the treatments caused any pre- or post-spawning mortality in the broodstock. Fertilization, eyeing and hatching percentages of the produced progeny were normal in all the treatment groups and did not differ significantly among them. In conclusion, FIA-emulsified GnRHa can be effective in advancing the onset of and synchronizing the ovulation of rainbow trout within a two-week period, thus shortening the egg collection period, without affecting broodstock survival and egg quality.  相似文献   

Retracted: Study of testis histology and sperm morphology in the Bunnei,Barbus sharpeyi (Gunther, 1874) induced by luteinizing hormone‐releasing hormone analogue and carp pituitary extract          下载免费PDF全文

Mohammad R Kalbassi  Reza Lorestani  Mohammad S Aramli 《Aquaculture Research》2017,48(2):473-480

This study was conducted to determine the effects of hormone treatment on testis structure in Barbus sharpeyi, as well as the morphology of sperm examined by scanning electronic microscopy (SEM). Male B. sharpeyi were divided into three groups (three fish per group) and injected with luteinizing hormone – releasing hormone analogue (LHRH–A₂) or carp pituitary extract (CPE). The first and second groups were treated with 10 μg kg^?1 LHRH–A₂ and metoclopramide (MET), and their testis were sampled pre‐ and Poststripping respectively. The third group received 2 mg kg^?1 CPE and were killed pre‐stripping. Based on the histological results obtained, the testicular connective tissue of the lumen was thicker, and seminal vesicles were of a lower volume, in fish injected with CPE in comparison to the other groups. After treatment with LHRH–A₂ and MET, not all spermatozoa within the testis were ejaculated, and only a small amount of sperm was obtained by abdominal stripping. The highest and lowest diameters of connective tissue within lobules were observed in fish receiving CPE and LHRH–A₂ treatments respectively. There was a significant difference (P < 0.05) in lobule space between the fish injected with the CPE and the fish injected with the LHRH–A₂ and MET. The SEM results revealed that the spermatozoa of B. sharpeyi were composed of a spherical to elliptical head, a cylindrical midpiece, and a lengthy flagellum. In conclusion, it was found that injection with LHRH–A₂ and MET improved the spermatogenic process in comparison to injection with CPE.  相似文献   

机械运输对伊朗北部山林陡峭集材道土壤的干扰     

Ramin Naghdi  Iraj Bagheri  Reza Basiri 《林业研究》2010,21(4):497-502

A study was conducted to investigate the effects of skid trail slope and traffic levels on soil disturbances at two soil depths (0–10 and 10–20 cm). The treatments were set at four traffic levels (2, 7, 12 and 20), two slope classes (<20% and >20%) and two soil depths (0–10 and 10–20 cm). Results show that skidder traffic, longitudinal slope and soil depth have significant effect on soil bulk density in skid trail. Comparison of average soil bulk density in different traffic levels shows that there are significant differences in average bulk density between different traffic levels and control (p<0.05). The average bulk densities in different slopes and soil depths are significantly increased with increase in traffic levels, maximized at 12 passes (p<0.05), but there are no significant differences between 12 and 20 passes. The interaction effects between traffic and soil depth are significant (F _0.05,3=0.109, p<0.001). For all traffic treatments, there are significant differences in soil moisture content between the two slope classes and the two depths (p<0.001). However, the interaction effects between traffic levels and slope classes are not significant (p >0.05), although skidder traffic and slope affected soil moisture content.  相似文献   

Effect of exogenous putrescine on post-harvest life of strawberry (Fragaria ananassa Duch.) fruit,cultivar Selva     

Mohammad Reza Zokaee Khosroshahi  Mahmood Esna-AshariAhmad Ershadi 《Scientia Horticulturae》2007

Effect of different concentrations of putrescine on post-harvest life of strawberry (Fragaria ananassa Duch.) fruit, cultivar Selva at 5 °C was studied. Fruits were immerged in 0.3, 0.5, 1 and 2 mM putrescine as well as distilled water (control) for 5 min, then transferred into the fridge (5 °C) together with untreated fruits (dry treatment). The rate of weight loss, ethylene production, flesh firmness, soluble solids content, titratable acidity and pH of fruits were determined 5, 9 and 13 days after the beginning of storage. Flesh firmness, appearance, color change and taste of fruits were also determined in the same intervals using a taste panel. Storage life of the strawberry fruits was significantly increased by the use of putrescine, so that the untreated and control fruits had 6 and 8 days storage life, respectively, while the immerged fruits in 1 and 2 mM putrescine were still suitable to be exposed in the market 12 and 14 days after the beginning of storage, respectively. No significant weight losses were observed in treated fruits compared to controls and dry treatment at all determination times. Ethylene production was decreased significantly by the use of putrescine. Untreated fruits (dry treatment) had the highest rate of ethylene production and the lowest rate was occurred in 2 mM putrescine treatment at all determination times (5, 9 and 13 days after the beginning of storage). The use of putrescine also prevented the softening of fruit flesh during the storage and kept their firmness, so that, the 2 mM putrescine treatment caused the highest fruit firmness at all determination times. Distilled water treatment (control) had the lowest fruit firmness 5 and 9 days after storage, while this occurred for the dry treatment 13 days after storage. Soluble solids content, pH and titratable acidity of the fruits were not significantly affected by the use of putrescine, but the highest and lowest rate of titratable acidity were related to the 2 mM putrescine and dry treatment, respectively, at the three determination times. Overall, the quality of fruits was improved by the use of 2 mM putrescine in terms of properties evaluated by the taste panel.  相似文献   

Antinociceptive diterpene from Euphorbia decipiens     

Ahmad VU  Hussain H  Bukhari IA  Hussain J  Jassbi AR  Dar A 《Fitoterapia》2005,76(2):230-232

One myrsinol-type diterpene ester (1) isolated from Euphorbia decipiens was evaluated for analgesic activity in the acetic acid induced writhing test in mice. Different dose (5-20 mg/kg i.p.) of the compound showed significant antinociceptive activity, which was comparable to standard analgesic drugs, aspirin and ibuprofen (100 mg/kg i.p.).  相似文献   

Analysis of HLA-G gene expression in B-lymphocytes from chronic lymphocytic leukemia patients   总被引：1，自引：0，他引：1  

Rezvany MR  Kazemi A  Hajifathali A  Kaviani S  Mellstedt H 《Iranian Biomedical Journal》2007,11(2):125-129

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Herbicide risk assessment during the Wheat Self-sufficiency Project in Iran     

Deihimfard R  Zand E  Damghani AM  Soufizadeh S 《Pest management science》2007,63(10):1036-1045

BACKGROUND: The present study describes the quantitative changes in herbicide use during the specific observation periods in the Wheat Self-sufficiency Project in Iran from 1994 to 2004 and the associated changes in herbicide risk, area and yield in this context. A risk index, the environmental impact quotient (EIQ), was used to estimate the environmental impacts (EIs) of herbicides applied to wheat agroecosystems. RESULTS: The results suggest that, during this period, the overall risk posed by the herbicides applied to wheat agroecosystems increased substantially, as evidenced by a 71% increase in herbicide usage (weight of pesticide applied) and a 62.2% increase in EI rating, in spite of an 8.2% decrease in overall EIQ rating and an 89.2% decrease in mean application rate. Furthermore, a 0.57% increase in the area and a 23.6% and 22.7% increase in irrigated and rainfed yields, respectively, were observed. The results also indicate that the EIQ(FUR) of two herbicides, dichlorprop-P/mecoprop-P/MCPA and difenzoquat, is much higher than that of other herbicides, and they could be qualified as high-risk herbicides. CONCLUSION: As a whole, an increase in herbicide usage (weight of pesticide applied) was an important factor on herbicide environmental impact boost. However, this increase has not led to a similar increase in wheat yield, which could be attributed in part to the negative impact of high herbicide consumption in wheat fields and subsequent threat to the long-term sustainability of these agroecosystems.  相似文献   

Chemical composition and in vitro antifungal activity of Sambucus ebulus and Actinidia deliciosa on the fish pathogenic fungus,Saprolegnia parasitica     

Mirmazloomi  Sina  Ghiasi  Maryam  Khosravi  Ali Reza 《Aquaculture International》2022,30(2):1037-1046

The aim of the present study was to determine the chemical composition of Sambucus ebulus and Actinidia deliciosa ethanolic extracts as well as their in vitro antifungal activity on the mycelial growth of the water mold, Saprolegnia parasitica. The preliminary minimum inhibition concentration (MIC) and minimum lethal concentration (MLC) were determined by the HeMP method and finally, concentrations of each extract ranging from 1 to 10% were prepared by an agar dilution method to assess the in vitro antifungal activity, quantitatively. Both herbal extracts inhibited growth of Saprolegnia hyphae in vitro. Complete in vitro growth inhibition was found at a concentration of ≥5% for S. ebulus, whereas it was not observed even at 10% concentration of A. deliciosa. Based on gas chromatography coupled with mass spectrometry (GC/MS) analysis, the main constituents of S. ebulus include monophthalate (66.17%), fatty acids (26.47%), phytol (4.25%), and acetic acid (2.11%). Using colorimetric assays, A. deliciosa contained phenolic contents at 162 mg gallic acid (GAE)/g DW and flavonoid contents at 2.31 mg quercetin (QE)/g DW. In conclusion, the results of this study indicated that S. ebulus and A. deliciosa showed some antifungal activities against S. parasitica with formerly exhibiting stronger activity (p < 0.05).

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Molecular typing of Iranian field isolates Mycoplasma synoviae and their differentiation from the live commercial vaccine strain MS-H using vlhA gene     

Mohammad Ali Bayatzadeh  Abass Ashtari  Ali Reza Abtin  Mohammad Abdoshah 《British poultry science》2014,55(2):148-156

1. The single-copy domain of the N-terminal region of the vlhA gene of Mycoplasma synoviae was sequenced, analysed and verified and used to type Iranian field isolates of M. synoviae and the MS-H live vaccine strain. In addition, a restriction fragment length polymorphism (RFLP) method was developed to differentiate between field isolates of Iranian and MS-H vaccine strains.

2. All sequences were analysed and aligned; the percentage similarity of the DNA was calculated and dendrograms were constructed. Based on single nucleotide polymorphism (SNP) that existed in all field isolates in Iran, the PCR-RFLP method allowed the differentiation of all M. synoviae field isolates from the vaccine strain.

3. Using phylogenetic analysis, the isolates were assigned to 8 unique genotypes and, within each group, DNA had a high level of similarity.

4. DNA sequence analysis and PCR-RFLP of the amplicon based on percent similarity and evolutionary relationship appeared to be useful tools for strain differentiation whether M. synoviae clinical isolates from infected chickens were derived from the vaccine strain or wild-type strains.

5. This study confirms the potential value of strain typing for epidemiological purposes and suggests that phylogenetic studies are essential to understand the true relationships between strains.  相似文献