Anthelmintic resistance in gastrointestinal nematodes of dairy cattle in the Macalister Irrigation District of Victoria          下载免费PDF全文

SL Bullen  DS Beggs  PD Mansell  DJ Runciman  J Malmo  MC Playford  MF Pyman 《Australian veterinary journal》2016,94(1-2):35-41

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Quantification of mucosa-adhered microbiota of lambs and calves by the use of culture methods and fluorescent in situ hybridization coupled with flow cytometry techniques     

Collado MC  Sanz Y 《Veterinary microbiology》2007,121(3-4):299-306

The intestinal mucosa-associated microbiota could play important biological roles due to its close proximity with the animal host, but knowledge on its composition is still limited. The aim of this study was to characterize the microbial communities tightly associated with different parts (rumen, duodenum and colon) of the gastrointestinal tract (GIT) of healthy lambs and calves by using both cultural, and fluorescent in situ hybridization-flow cytometry (FCM-FISH) techniques. Lactic acid bacteria genera were one of the predominant bacteria detected in lambs and calves by both methodologies, possibly constituting an index of their healthy status. The levels of Lactobacillus were significantly higher (p<0.05) in the rumen and duodenum of lambs, and in the rumen of calves. The levels of Bifidobacterium were significantly higher (p<0.05) in the colon of both animal species and the rumen of lambs. Significant differences (p<0.05) were found in counts of other microbial groups (yeast, Enterococcus, Propionibacterium, Bacteroides, Clostridium and Enterobacteriaceae) at diverse GI sections depending on the animal species. In general, microbial counts follow the same trends regardless the applied technique. The most remarkable differences were found in detection levels of Bacteroides and Clostridium, which tended to be significantly higher (p<0.05) when analysed by FCM-FISH. This technique also allowed the detection of quantitatively important bacteria (sulphate-reducing bacteria, Atopobium and Coriobacterium), which are difficult to cultivate in selective medium. Therefore, FCM-FISH has been proven to be a sensitive high throughput approach that provides additional information to that obtained by traditional culture techniques about the complexity of the GI ecosystem of these animal species.  相似文献   

Comparative study of digestive enzymes in fish with different nutritional habits. Proteolytic and amylase activities   总被引：27，自引：0，他引：27  

M. C. Hidalgo  E. Urea  A. Sanz 《Aquaculture (Amsterdam, Netherlands)》1999,170(3-4):267-283

This work provides a comparative study of the proteolytic and amylase activities in six species of fish with different nutritional habits: rainbow trout (Oncorhynchus mykiss), gilthead seabream (Sparus aurata), European eel (Anguilla anguilla), common carp (Cyprinus carpio), goldfish (Carassius auratus), and tench (Tinca tinca). Trout and carp showed the highest digestive proteolytic activity. When proteolytic activity was determined in a wide range of pHs, the highest values in the digestive tract of all species were found at alkaline pHs, except in eel where activity could be detected only at acid pHs. Eel showed the lowest digestive proteolytic potential among all the species studied. With respect to amylase activity, the omnivorous species presented higher activity than did the carnivores. Among the carnivorous species, the lowest activity was found in trout. The ratio of total amylase:total proteolytic activity was higher in omnivorous fish species, the carp having the greatest value, whereas in trout this ratio was lower than one. Digestive enzyme activity declined as the incubation temperature decreased, but this trend varied depending on the fish species and the tissue analyzed.  相似文献   

Ecoviability for ecosystem‐based fisheries management          下载免费PDF全文

Luc Doyen  Christophe Béné  Michel Bertignac  Fabian Blanchard  Abdoul Ahad Cissé  Catherine Dichmont  Sophie Gourguet  Olivier Guyader  Pierre‐Yves Hardy  Sarah Jennings  Lorne Richard Little  Claire Macher  David Jonathan Mills  Ahmed Noussair  Sean Pascoe  Jean‐Christophe Pereau  Nicolas Sanz  Anne‐Maree Schwarz  Tony Smith  Olivier Thébaud 《Fish and Fisheries》2017,18(6):1056-1072

Reconciling food security, economic development and biodiversity conservation is a key challenge, especially in the face of the demographic transition characterizing many countries in the world. Fisheries and marine ecosystems constitute a difficult application of this bio‐economic challenge. Many experts and scientists advocate an ecosystem approach to manage marine socio‐ecosystems for their sustainability and resilience. However, the ways by which to operationalize ecosystem‐based fisheries management (EBFM) remain poorly specified. We propose a specific methodological framework—viability modelling—to do so. We show how viability modelling can be applied using four contrasted case‐studies: two small‐scale fisheries in South America and Pacific and two larger‐scale fisheries in Europe and Australia. The four fisheries are analysed using the same modelling framework, structured around a set of common methods, indicators and scenarios. The calibrated models are dynamic, multispecies and multifleet and account for various sources of uncertainty. A multicriteria evaluation is used to assess the scenarios’ outcomes over a long time horizon with different constraints based on ecological, social and economic reference points. Results show to what extent the bio‐economic and ecosystem risks associated with the adoption of status quo strategies are relatively high and challenge the implementation of EBFM. In contrast, strategies called ecoviability or co‐viability strategies, that aim at satisfying the viability constraints, reduce significantly these ecological and economic risks and promote EBFM. The gains associated with those ecoviability strategies, however, decrease with the intensity of regulations imposed on these fisheries.  相似文献   

Stand factors influencing Pinus halepensis decline in north‐western Spain          下载免费PDF全文

A. V. Sanz‐Ros  F. Valladares  J. J. Diez 《Forest Pathology》2016,46(4):309-317

Although decline of Aleppo pine was observed long ago and several climatic and biotic factors have been previously associated with this complex process, site factors involved in this decline remain poorly understood. The objective of the work described here was to identify site factors associated with canopy condition. Canopy condition was estimated both by a visual estimation of defoliation, and by an indirect estimation of leaf area index (LAI) and other stand‐ and light‐related parameters through the analysis of hemispherical photographs. A high percentage of damaged trees (81%) along with high levels of defoliation in plots (up to 53%) and trees (up to 85%) were recorded. Regression models showed that the site factors associated with defoliation were basal area, age, crown depth and elevation, while those associated with LAI were diameter at breast height, tree density and canopy openness. Analysis of hemispherical photographs proved to be a useful method for LAI estimation, but not for estimation of defoliation due to heterogeneous defoliation patterns caused by fungal pathogens detected in the study area. Soils and climatic conditions were common to all plots, so their influence could not be tested, but poor soil conditions and climatic restraints are known in this area, including low soil productivity, frequent summer droughts and high numbers of frost days. The results obtained suggest that several factors were associated with the decline of Pinus halepensis, including age, basal area, canopy openness, diameter, height and tree density. These factors can influence canopy condition, and thus, they might be acting as predisposing factors for the decline. The modulation of these factors is possible if suitable forest management strategies are applied, which could lead to a decrease of the decline incidence.  相似文献   

Structural Characterization of New Peptide Variants Produced by Cyanobacteria from the Brazilian Atlantic Coastal Forest Using Liquid Chromatography Coupled to Quadrupole Time-of-Flight Tandem Mass Spectrometry     

Miriam Sanz  Ana Paula Dini Andreote  Marli Fatima Fiore  Felipe Augusto D?rr  Ernani Pinto 《Marine drugs》2015,13(6):3892-3919

Cyanobacteria from underexplored and extreme habitats are attracting increasing attention in the search for new bioactive substances. However, cyanobacterial communities from tropical and subtropical regions are still largely unknown, especially with respect to metabolite production. Among the structurally diverse secondary metabolites produced by these organisms, peptides are by far the most frequently described structures. In this work, liquid chromatography/electrospray ionization coupled to high resolution quadrupole time-of-flight tandem mass spectrometry with positive ion detection was applied to study the peptide profile of a group of cyanobacteria isolated from the Southeastern Brazilian coastal forest. A total of 38 peptides belonging to three different families (anabaenopeptins, aeruginosins, and cyanopeptolins) were detected in the extracts. Of the 38 peptides, 37 were detected here for the first time. New structural features were proposed based on mass accuracy data and isotopic patterns derived from full scan and MS/MS spectra. Interestingly, of the 40 surveyed strains only nine were confirmed to be peptide producers; all of these strains belonged to the order Nostocales (three Nostoc sp., two Desmonostoc sp. and four Brasilonema sp.).  相似文献   

Immunohistochemical Localization of RANK, RANKL and OPG in Healthy and Arthritic Canine Elbow Joints     

ANDREA I. SPAHNI  Dr. med. vet.    PETER SCHAWALDER  Prof. Dr. med. vet.    BARBARA ROTHEN  PD Dr. sc. nat. ETH    DIETER D. BOSSHARDT  PD Dr. sc. nat.  PhD    NIKLAUS LANG  Prof. Dr. med. dent.    MICHAEL H. STOFFEL  Prof. Dr. med. vet. 《Veterinary surgery : VS》2009,38(6):780-786

Objective— To determine if the receptor activator of nuclear factor-κB–receptor activator of nuclear factor-κB ligand–osteoprotegerin (RANK–RANKL–OPG) system is active in bone remodeling in dogs and, if so, whether differences in expression of these mediators occur in healthy and arthritic joints.
Study Design— Experimental study.
Sample Population— Fragmented processus coronoidei (n=20) were surgically removed from dogs with elbow arthritis and 5 corresponding healthy samples from dogs euthanatized for reasons other than elbow joint disease.
Methods— Bright-field immunohistochemistry and high-resolution fluorescence microscopy were used to investigate the distribution of RANK, RANKL, and OPG in healthy and arthritic joints.
Results— All 3 molecules were identified by immunostaining of canine bone tissue. In elbow dysplasia, the number of RANK-positive osteoclasts was increased. In their vicinity, cells expressing RANKL, a mediator of osteoclast activation, were abundant whereas the number of osteoblasts having the potential to limit osteoclastogenesis and bone resorption via OPG was few.
Conclusions— The RANK–RANKL–OPG system is active in bone remodeling in dogs. In elbow dysplasia, a surplus of molecules promoting osteoclastogenesis was evident and is indicative of an imbalance between the mediators regulating bone resorption and bone formation. Both OPG and neutralizing antibodies against RANKL have the potential to counterbalance bone resorption.
Clinical Relevance— Therapeutic use of neutralizing antibodies against RANKL to inhibit osteoclast activation warrants further investigation.  相似文献   

Comparative chemistry and insect antifeedant action of traditional (Clevenger and Soxhlet) and supercritical extracts (CO₂) of two cultivated wormwood (Artemisia absinthium L.) populations     

Luis MartínLuis F. Julio  Jesus BurilloJesus Sanz  Ana M. MainarAzucena González-Coloma 《Industrial Crops and Products》2011,34(3):1615-1621

A comparison between traditional extraction techniques (hydrodistillation and organic solvent extraction) and supercritical fluid extraction was made for two different populations and crops of Artemisia absinthium L., cultivated in the field and aeroponically. The composition of the extracts, volatile and non volatile oils, was analyzed by GC-MS and HPLC-DAD, respectively. The antifeedant and phytotoxic activity of the extracts was tested on insect pests (Spodoptera littoralis, Myzus persicae and Rhopalosiphum padi) and plants (Lactuca sativa and Lolium perenne). The supercritical extracts exhibited stronger antifeedant effects than the traditional ones (up to 8 times more active) with moderate selective phytotoxic effects on L. perenne root growth (<50% inhibition).  相似文献   

Vineyard zone delineation by cluster classification based on annual grape and vine characteristics     

Ana?Belén?González-FernándezEmail author  José?Ramón?Rodríguez-Pérez  Enoc?Sanz?Ablanedo  Celestino?Ordo?ez 《Precision Agriculture》2017,18(4):525-573

This study describes a method for vineyard zone delineation based on spatial interpolation of data on annual monitoring of grape and vine growth from 2007 to 2012 for four commercial vines (Cabernet Sauvignon, Mencía, Merlot and Tempranillo) located in the Bierzo Denomination of Origen (NW Spain). A sampled grid of 20 × 29 m (14 vines/ha) was defined for each vineyard and data were collected for ten soil, six grape composition, three grape production and five vine vigour variables. Continuous maps of each variable were created by spatial interpolation from the sampled points. Several zone delineations were obtained by clustering—using the iterative self-organizing data analysis (ISODATA) algorithm—according to different combinations of the studied variables. The resulting zone delineations were analysed (ANOVA) in order to determine whether the variables in the two cluster classifications for two or three zones were statistically different from each other. The selected delineation was the cluster that included total soluble solids, titratable acidity, total phenolic content, pH, mean cluster weight and length of the internode in two zones. The results point to the feasibility of this approach to vineyard zone delineation. Further research is necessary to confirm the effectiveness of this approach for other locations and evaluate the usefulness of introducing new grape and vine variables.  相似文献   

Participation of phosphofructokinase,malate dehydrogenase and isocitrate dehydrogenase in capacitation and acrosome reaction of boar spermatozoa          下载免费PDF全文

E Breininger  D Dubois  VE Pereyra  PC Rodriguez  MM Satorre  PD Cetica 《Reproduction in domestic animals》2017,52(5):731-740

The aim of this work was to determine the enzymatic activity of phosphofructokinase (PFK), malate dehydrogenase (MDH) and isocitrate dehydrogenase (IDH) in boar spermatozoa and study their participation in bicarbonate‐induced capacitation and follicular fluid‐induced acrosome reaction. Enzymatic activity of these enzymes was determined spectrophotometrically in extracts of boar spermatozoa. Sperm suspensions were incubated in the presence of bicarbonate (40 mM), a well‐known capacitation inducer, or follicular fluid (30%), as an acrosome reaction inducer, and different concentrations of oxoglutarate, oxalomalate and hydroxymalonate, inhibitors of PFK, IDH and MDH, respectively. Capacitation percentages were determined by the fluorescence technique of chlortetracycline (CTC), and true acrosome reaction was determined by trypan blue and differential–interferential contrast, optical microscopy. The activity of PFK in boar spermatozoa enzymatic extracts was 1.70 ± 0.19 U/10¹⁰ spermatozoa, the activity of NAD‐ and NADP‐dependent IDH was 0.111 ± 0.005 U/10¹⁰ and 2.22 ± 0.14 U/10¹⁰ spermatozoa, respectively, and the activity of MDH was 4.24 ± 0.38 U/10¹⁰ spermatozoa. The addition of the specific inhibitors of these enzymes prevented sperm capacitation and decreased sperm motility during capacitation and inhibited the acrosome reaction (AR), without affecting the sperm motility during this process. Our results demonstrate the participation of PFK, IDH and MDH in bicarbonate‐induced capacitation and follicular fluid‐induced acrosome reaction in boar spermatozoa, contributing to elucidate the mechanisms that produce energy necessary for these processes in porcine spermatozoa.  相似文献