<Emphasis Type="Italic">In situ</Emphasis> conservation and genetic diversity of three populations of <Emphasis Type="Italic">Gossypium mustelinum</Emphasis> Miers ex Watt     

Paulo Augusto Vianna Barroso  Lúcia Vieira Hoffmann  Rodolfo Barbosa de Freitas  Carlos Eduardo de Araújo Batista  Milena Ferreira Alves  Uiara Cavalcante Silva  Francisco Pereira de Andrade 《Genetic Resources and Crop Evolution》2010,57(3):343-349

Gossypium mustelinum Miers ex Watt is a native from Northeastern Brazil and belongs to the primary gene pool of the cultivated cotton. The unique places where the species was known to occur were visited to plan ex situ and in situ preservation. Two populations at Caicó became extinct, and only eleven individuals were found. At Jaguarari, although one population became extinct, a new one was localized with approximately 500 adult plants, where a fence avoided cattle feeding. The population from Macururé consisted of 28 plants protected from animals by thorns of Bromelia laciniosa Mart. ex Schult. f. Thirteen SSR primer pairs amplified 40 alleles, among which 30% were exclusive of one of the populations. The genetic differences between populations represented 58.3% of the total genetic variation observed. The high genetic distances are likely to be caused by geographical isolation as well as by the small number of individuals which contribute to new generations, and consequential genetic drift.  相似文献   

Vaccination strategies with oral booster for surubim hybrid (Pseudoplatystoma corruscans × P. reticulatum) against haemorrhagic septicaemia          下载免费PDF全文

Gabriella do Vale Pereira  Bruno Corrêa da Silva  Felipe do Nascimento Vieira  Walter Quadros Seiffert  Thiago Tetsuo Ushizima  José Luiz Pedreira Mouriño  Maurício Laterça Martins 《Aquaculture Research》2015,46(8):1831-1841

This study evaluated the efficiency of differently prepared vaccines against Aeromonas hydrophila in the hybrid surubim (Pseudoplatystoma corruscans × P. reticulatum). Survival and haemato‐immunological parameters were compared between the treatments: non‐vaccinated fish (C); bacterin‐vaccinated fish (B); bacterin plus oral booster vaccinated fish (B+O); bacterin and toxoid‐vaccinated fish (B+T) and bacterin, toxoid and oral booster‐vaccinated fish (B+T+O). Fourteen‐days vaccinated fish from B+O and B+T+O were fed with an oral booster for 4 days. After 1 week, the fish were intraperitoneally challenged with 2 × 10⁸ CFU mL^?1 of A. hydrophila. Fish from the treatment B+T+O showed the lowest cumulative mortality (11.36%) 96 h after challenge, compared with other treatments (22.72–44.04%), and a relative survival of 74%. Serum immunoglobulin in B+T+O fish was higher than in other treatments. All vaccinated fish showed an increased agglutination titre when compared with non‐vaccinated fish, both before and after challenge. Fish fed with oral booster showed an increase in phagocytic percentage before and after challenge. It can be inferred that the oral booster vaccination was efficient in reducing mortality in hybrid surubim by enhancing the response against haemorrhagic septicaemia due to A. hydrophila infection.  相似文献   

Studies on the colonization of axenically grown tomato plants by a GFP-tagged strain of Clavibacter michiganensis subsp. michiganensis     

Flávia M. Vieira Lelis  Robert Czajkowski  Ricardo Magela de Souza  Daniel H. Ribeiro  Jan M. van der Wolf 《European journal of plant pathology / European Foundation for Plant Pathology》2014,139(1):53-66

In this study, colonization and disease development of axenically-grown tomato plants by Clavibacter michiganensis subsp. michiganensis (Cmm), the causative agent of bacterial wilt and canker, was investigated. For this, a spontaneous rifampicin resistant strain of Cmm was tagged with a marker that expressed a green fluorescent protein (GFP) in a stable way and which possessed a similar virulence to the parental strain. In vitro plants were drop-inoculated at the stem base and the population dynamics was determined by dilution pour-plating in a selective medium. At 3 h after inoculation, Cmm was already present in low densities in roots, stems and leaves. At 16 dpi, Cmm was found throughout the entire plant in high densities of ca. 10¹⁰ cfu g^?1. Symptoms developed in the in vitro plants typical for Cmm, such as canker, wilting and growth reduction. The presence of Cmm in vascular and parenchymatic tissue of in vitro tomato plants was confirmed by epifluorescence stereo- and confocal laser scanning microscopy. This study showed that in vitro tomato plants can be effectively used for detailed studies on interactions between Cmm and its host, in particular if a GFP-tagged strain of the pathogen is used.  相似文献   

High?throughput phenotyping of two plant?size traits of Eucalyptus species using neural networks     

Marcus Vinicius Vieira Borges  Janielle de Oliveira Garcia  Tays Silva Batista  Alexsandra Nogueira Martins Silva  Fabio Henrique Rojo Baio  Carlos Ant?nio da Silva Junior  Gileno Brito de Azevedo  Glauce Taís de Oliveira Sousa Azevedo  Larissa Pereira Ribeiro Teodoro  Paulo Eduardo Teodoro 《林业研究》2022,(2):591-599

In forest modeling to estimate the volume of wood, artificial intelligence has been shown to be quite effi-cient, especially using artificial neural networks (A...  相似文献   

Molecular detection of Anaplasma platys and Ehrlichia canis in dogs from the North of Portugal     

Luís Cardoso  Joana Tuna  Lisete Vieira  Yael Yisaschar-Mekuzas  Gad Baneth 《Veterinary journal (London, England : 1997)》2010,183(2):232-233

Tick-transmitted rickettsial pathogens belonging to the Ehrlichia and Anaplasma genera can infect dogs and humans. In this study, four dogs from the North of Portugal, in which an ehrlichial disease was suspected clinically, were tested by molecular methods. After DNA extraction from blood on filter paper, a 345 bp fragment of the Ehrlichia/Anaplasma 16S rRNA gene was amplified by the polymerase chain reaction (PCR). Sequence analysis of PCR products revealed one dog infected with Ehrlichia canis and three with Anaplasma platys. One of these latter animals was co-infected with Babesia canis subspecies vogeli. This is the first report of the genetic characterisation of both A. platys and E. canis in naturally infected dogs from the North of Portugal.  相似文献   

Cutaneous mycoflora and CD4:CD8 ratio of cats infected with feline immunodeficiency virus     

Archivaldo Reche  Alexandre G.T. Daniel  Telma C.P. Lazaro Strauss  Carlos P. Taborda  Shirlei A. Vieira Marques  Kátia Haipek  Lilian J. Oliveira  Janaína M. Monteiro  José R. Kfoury 《Journal of Feline Medicine and Surgery》2010,12(4):355-358

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Water submersion of seeds from three bean cultivars     

Geraldo Henrique Martins Vieira  Joice Yuri Minamiguchi  Edna Antonia Torquato de Agostini  Charlotte E. Seal  Angélica Augusta Grigoli  Nelson Barbosa Machado Neto 《Plant Production Science》2016,19(1):51-60

Tropical areas exhibit torrential rains, and when the rate of soil drainage is slower than the rate of precipitation, rapid flooding can result which can lead to germination failure. The objective of this study was to evaluate the tolerance of three bean cultivars (IPR-139, Pérola and IPR-Tiziu) to submersion in water for different time periods (0, 1, 2, 4, 8 and 16 h). Seeds were evaluated for moisture content, electrical conductivity, leached ethanol, germination percentage and vigour classification, length and dry weight of roots and shoots, proline content and superoxide dismutase (SOD) activity (an antioxidant enzyme). Increasing the submersion time resulted in decreased germination, vigour and growth, with increased electrical conductivity and leaching of ethanol. Just 1 h of submersion was enough for early differences to be evident among cultivars. The SOD activity was near constant during submersion in all cultivars. Overall, IPR-139 was the most susceptible to submersion, and IPR-Tiziu was the most tolerant cultivar. The tolerance of IRP-Tiziu was related to the high proline content in dry seeds and proline consumption during the submersion period.  相似文献   

Evaluation of Fusarium graminearum inoculation methods in maize ears and hybrid reaction to Gibberella ear rot under southern Brazilian environmental conditions     

Francine R. Nerbass  Ricardo T. Casa  Paulo Roberto Kuhnem  Amauri Bogo  Luis Sangoi  Maiquiel D. Fingstag  José A. L. Vieira Junior  Juan C. Stoltz 《European journal of plant pathology / European Foundation for Plant Pathology》2016,144(1):45-53

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Effects of different dietary lipid levels and fatty acids profile in the culture of white shrimp Litopenaeus vannamei (Boone) in biofloc technology: water quality,biofloc composition,growth and health          下载免费PDF全文

Tárik Massucci Toledo  Bruno Corrêa Silva  Felipe do Nascimento Vieira  José Luiz Pedreira Mouriño  Walter Quadros Seiffert 《Aquaculture Research》2016,47(6):1841-1851

The objective of this study was to evaluate the influence of different dietary lipid and fatty acids on the nutritional value of bioflocs used as a feed, as well as shrimp performance and health. A total of 1800 Litopenaeus vannamei juveniles (2.87 ± 0.01 g) were cultured in biofloc technology, with a density of 200 shrimp m^?2, and fed with three isoproteic experimental diets at different lipid levels (85 g kg^?1, 95 g kg^?1 and 105 g kg^?1); each treatment was performed in triplicate. After 61 days, no significant difference was observed (P > 0.05) among the water quality parameters. For the shrimp performance, significant difference was observed (P = 0.011) among the values of survival, where treatments with lower lipid levels had higher survival (92.5 ± 3.5% and 91.0 ± 2.5%). Although there are significant differences in survival, no significant differences in the total haemocytes count (THC) were observed. For other growth performance, no differences were observed (P > 0.05). A positive correlation (r = 0.75) has been observed between the dietary oleic acid and bioflocs. The bioflocs showed ‘long‐chain’ polyunsaturated fat acids (lcPUFA), especially arachidonic acid. The shrimp showed similar growth and stayed healthy at the end of the experimental period.  相似文献   

Spatial Variability of Soil Organic Matter and Cation Exchange Capacity in an Oxisol under Different Land Uses     

Jorge Paz Ferreiro  Vicente Pereira De Almeida  Marlene Cristina Alves  Cleide Aparecida De Abreu  Sidney R. Vieira 《Communications in Soil Science and Plant Analysis》2016,47(22):75-89

ABSTRACT

Soil properties may exhibit large spatial variability. Frequently this variability is auto-correlated at a certain scale. In addition to soil-forming factors, soil management, land cover, and agricultural system may affect the spatial variability of agricultural soils. Soil organic matter (OM) is an important soil property contributing toward soil fertility and a key attribute in assessing soil quality. Increasing soil OM increases cation exchange capacity (CEC) and enhances soil fertility. We analyzed the impact of land use on the spatial variability of OM and CEC in a tropical soil, an Oxisol, within São Paulo state, Brazil. Land uses were prairie, maize, and mango. Soil samples were taken at 0–10 and 10–20 cm depths at 84 points within 1-ha plots, i.e., 100 m × 100 m. Statistical variability was higher for soil OM than for CEC. The mango plot contained the highest soil OM, whereas prairie the lowest. Also, soil OM and CEC were significantly related at all land use treatments and depths studied. All soil OM data sets and most of the CEC data sets (with two exceptions) exhibited spatial dependence. When spatial variability was present, the semivariograms showed a nugget effect plus a spherical or an exponential structure. Patterns of soil OM and CEC spatial variability (i.e., model type, ranges of spatial dependence, and nugget effects) were different between land uses and soil depths. In general, CEC exhibited a lower spatial autocorrelation and a weaker spatial structure than soil OM. Moreover, soil OM displayed a higher autocorrelation and was more strongly structured at the 0–10 cm depth than at the 10–20 cm depth. Interpolation by kriging or inverse distance weighting (IDW) allowed to illustrate how the spatial variability of soil OM and CEC differed due to land cover and sampling depth. Modeling and mapping the spatial distribution of soil OM and CEC provided a framework for spatially implicit comparisons of these soil properties, which may be useful for practical applications.  相似文献