Identification of stable normalization genes for quantitative real-time PCP, in porcine articular cartilage     

Ryan S McCulloch  Melissa S Ashwell  Audrey T O’Nan  Peter L Mente 《畜牧与生物技术杂志(英文版)》2012,(4):181-187

Background: Expression levels for genes of interest must be normalized with an appropriate reference, or housekeeping gene, to make accurate comparisons of quantitative real-time PCR results. The purpose of this study was to identify the most stable housekeeping genes in porcine articular cartilage subjected to a mechanical injury from a panel of 10 candidate genes. Results: Ten candidate housekeeping genes were evaluated in three different treatment groups of mechanically impacted porcine articular cartilage. The genes evaluated were: beta actin, beta-2-microglobulin, glyceraldehyde-3-phosphate dehydrogenase, hydroxymethylbilane synthase, hypoxanthine phosphoribosyl transferase, peptidylprolyl isomerase A (cyclophilin A), ribosomal protein L4, succinate dehydrogenase flavoprotein subunit A, TATA box binding protein, and tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein-zeta polypeptide. The stability of the genes was measured using geNorm, BestKeeper, and NormFinder software. The four most stable genes measured via geNorm were (most to least stable) succinate dehydrogenase flavoprotein, subunit A, peptidylprolyl isomerase A, glyceraldehyde-3-phosphate dehydrogenase, beta actin; the four most stable genes measured via BestKeeper were glyceraldehyde-3-phosphate dehydrogenase, peptidylprolyl isomerase A, beta actin, succinate dehydrogenase flavoprotein, subunit A; and the four most stable genes measured via NormFinder were peptidylprolyl isomerase A, succinate dehydrogenase flavoprotein, subunit A, glyceraldehyde-3-phosphate dehydrogenase, beta actin. Conclusions: BestKeeper, geNorm, and NormFinder all generated similar results for the most stable genes in porcine articular cartilage. The use of these appropriate reference genes will facilitate accurate gene expression studies of porcine articular cartilage and suggest appropriate housekeeping genes for articular cartilage studies in other species.  相似文献   

Biodiversity of Arbuscular Mycorrhizal (AM) fungi in mangroves of Goa in West India     

James D’Souza  Bernard Felinov Rodrigues 《林业研究》2013,24(3):515-523

Seventeen mangrove species of eight families at seven riverine and fringe habitats in Goa West India were surveyed for Arbuscular Mycorrhizal (AM) fungal diversity. Sixteen species were found to be mycorrhizal and one species showed no AM fungal colonization. AM root colonization was recorded at all seven sites and ranged from 6%-77%. Maximum root colonization was recorded in Excoecaria agallocha (77%) and minimum colonization in Avicennia marina (6%). Paris-type colonization was predominant at all sites. Auxiliary cells were recorded in roots of Acanthus ilicifolius, Ceriops tagal and Sonneretia alba. AM fungal root colonization and spore density varied by plant species and site. Site average spore density ranged from 1.84 spores·g-1 to 0.54 spores·g-1 of soil. In total, 28 AM fungal species of five genera, viz. Glomus, Acaulospora, Scutellospora, Gigaspora and Entrophospora, were recovered. Glomus was the dominant genus, three species of which were sporocarpic forms. Maximum site species richness (SR) ranged from 16 to 5. Species richness was maximum in A. ilicifolius where seven species of three genera were recovered. Based on relative abundance (RA) and isolation frequency (IF), two common species, viz. G. intraradices and A. laevis, were recovered from all seven sites.  相似文献   

郁金散加味配合西药治疗野猪仔猪黄痢     

张加力’陈茹’于冰’温伟 《中国兽医杂志》2014,(4):50-51

<正>仔猪黄痢是新生仔猪的急性、高致死性传病。主要发生于1周龄内的新生仔猪,以1³日龄发病最多,随日龄的增长发病减少,1周龄以上很少发病。初产母猪所产仔猪发病最为严重,而经产母猪所产仔猪发病相对较轻。猪场卫生条件不好,新生仔猪初乳吃得不够或母猪乳汁不足以及产房温度  相似文献   

水提与醇提对竹子中愈创木酚含量的影响     

陈秋香’  蒋晓煌  李红 《勤云标准版测试》2011,31(9):46-48

〔摘要〕目的优选竹子中有效成分愈创术酚的最佳提取溶媒〔方法将新鲜竹子砍成小段,再粉碎成粗粉,加水 或}o%乙醇回流提取,提取液采用高效液相法测定愈创术酚含量、色谱条件为:流动相0.50IcH,PO、的水溶液一乙睛 (78:22);固定相为C,、柱;流速1.0 mLJmin;柱温20 0C ;检测波长220 nm〔结果醇提法愈创术酚含量略高于水提法, 但无明显差异、结论水提法可以代替醇提法、  相似文献   

毛叶丁香上两种食叶害虫的区别与防控     

吴红娟  牟灿  李文娟  张人月  汪桔  张晓娟 《南方农业》2018,(22)

2017年在对园林植物病虫害发生情况调查时发现,重庆市巴南区毛叶丁香上存在两种主要食叶害虫,即女贞潜叶跳甲(Argopistes tsekooni Chen)和黄环绢须野螟(Palpita annulata Fabricius)。通过室外调查、室内饲养及观察,并结合文献资料,从为害虫态、为害寄主范围、为害状、害虫形态及发生规律几个方面对二者进行了区别和描述,并提出针对性的防控措施。  相似文献   

胎膜早破1120例临床分析     

吴红娟 《湛江医学院学报》2009,27(6):672-673

目的分析胎膜早破的临床处理及母婴结局。方法回顾性分析近3年因胎膜早破而在本院住院分娩的1120孕妇及其围产儿的临床资料。结果本组早产率为10.0%,感染率、胎儿宫内窘迫发生率分别为1.8%和12.9%。结论对胎膜早破者应积极控制感染,预防并发症的发生。  相似文献   

小儿手足口病辨证论治方案专家问卷调查分析     

詹雯’.土孟清 《勤云标准版测试》2011,31(3):66-68

〔摘要〕目的明确小儿手足ii病辨证论治治法、方药、内服、外用、中药注射液,为进一步制定科学、客观的小儿 辨证论治方案提供参考、方法根据文献研究结果,通过德尔菲法制定问卷,利用SPSS软件对数据进行积极度、集中 程度、协调程度、权威程度的统计与评价〔结果专家对7个证候对应的治法、方药协调程度一致,对2个中药注射 液、4个}}服中成药、3个外用中成药协调程度一致、结论专家问卷调查是分析小儿辨证论治方案的有效方法、  相似文献   

盐酸青藤碱加离子导入法 对透皮吸收的影响     

邱赛红’  李琳’  朱传湘’  吴红娟’  滕健  谢昭明 《勤云标准版测试》2011,31(3):36-38

〔摘要〕目的研究盐酸青藤碱的透皮吸收及离子导入方法对透皮吸收的影响、方法采用家兔离体皮肤及 Franz扩散池进行体外透皮试验,并以电子治疗仪作为外加电场进行离子导入,观测盐酸青藤碱注射液的透皮渗透 率,并比较外加电场对其透皮渗透量的影响、结果盐酸青藤碱的透皮量随着时间的延长而增加,符合零级动力学 方程( R==0.952 1)、外加电场可增加其透皮吸收量,其中以电场强度20 mA、通电时间15 min的透皮量较为I}想、 结论盐酸青藤碱注射液可透皮吸收,电场导入对其透皮吸收有一定的促进作用、木研究为临床中以盐酸青藤碱注 射液进行离子导入的治疗方法提供了实验依据、  相似文献   

滋阴活血解毒方对局灶性脑缺血模型大鼠 血清中IL-1(3,IL-8,TNF-。含量的影响     

段春艳’.陈元.李定祥.邓奕辉 《勤云标准版测试》2011,31(1):36-39

〔摘要〕目的观察滋阴活血解毒饮片及超微方对大鼠局灶性脑缺血后白细胞介素一1日(IL-1日)、白细胞介素- 8(IL-8)、肿瘤坏死因子一。(TNF-a)含量的影响、方法将25只大鼠随机分为假手术组、模型组、滋阴活血解毒方饮 片组(以卜简称饮片组)、滋阴活血解毒方超微组(以卜简称超微组)、阿加曲班组,每组5只、大脑中动脉线栓法复 制局灶性脑缺血模型,进行神经功能评分〔造模成功后7d,用TTC染色法测定脑梗死范围,用Motic Med6.0数码 图像分析系统分析脑梗死面积〔采用ELISA法测定大鼠血清IL-1日,IL-8 ,TNF-。的含量〔结果饮片组、超微组及 阿加曲班组大鼠脑梗死体积比均明显低于模型组,差异具有统计学意义(P<0.01) ;模型组血清IL-1日,IL-8,TNF-a 含量明显高于假手术组(P<0.01),饮片组、超微组、阿加曲班组均能明显降低大鼠血清IL-1日、IL-8 , TNF-a含量, 与模型组比较,差异均有统计学意义(P<0.01)、结论滋阴活血解毒方能减小脑梗死面积,抑制炎性因子的表达,降 低脑缺血所致的脑损伤、  相似文献   

昆明地区高血压人群中医体质分布特点调查分析     

金艳蓉’  江建锋’  李军’  杨海燕’  周志娴’  土庆淑’  马黎 《勤云标准版测试》2011,31(7):77-79

〔摘要〕目的初步了解昆明市部分社区高血压及其合并心衰人群的中医体质类型分布状况,探讨中医体质与 高血压合并心衰发病的相关性〔方法采用标准化的9种中医体质量表对高血压合并心衰人群进行横断面现状调 查〔结果所有调查414例有效问卷中,高血压人群376例,正常对照组38例〔其中高血压人群平和质117例,单纯 偏颇体质86例,兼夹偏颇体质173例〔所有人群气虚质频次最多,且正常血压、高血压及高血压合并心衰人群中医 体质分布比较,差异均具有统计学意义(P<0.05 )、结论中医体质具有其复杂性,气虚体质在木地区老年人高血压患 者存在普遍性.阳虚质在致高血压心衰中具有决定性.疲血质及痰湿质也是致高血压合并心衰发病的重要体质、  相似文献