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Plant breeding has developed perennial ryegrass varieties with increased concentrations of water‐soluble carbohydrates (WSCs) compared with conventional varieties. Water‐soluble carbohydrates are major metabolic and storage components in ryegrass. Therefore, if perennial ryegrass herbage is allowed to grow to greater heights it should contain higher water‐soluble carbohydrates concentrations, for example as under rotational grazing rather than continuous grazing by livestock. This study investigated this hypothesis and measured the performance of lambs grazed rotationally and continuously. Replicated plots of the variety AberDart (bred to express high WSC concentrations) or the variety Fennema were grazed by a core group of ten male Cheviot lambs for 10 weeks. Lambs were weighed and replicate forage samples were taken every 7 d. Concentrations of WSC in AberDart herbage were significantly (P < 0·05), but not substantially, higher than those in Fennema herbage. Rotational grazing did not increase the differential in WSC concentration between the AberDart and Fennema varieties. However, there was a tendency (P = 0·07) for lambs rotationally grazing the AberDart swards to have a higher final live weight than lambs grazing the Fennema swards. Overall, lamb performance was increased when either perennial ryegrass variety was rotationally rather than continuously grazed (P < 0·001).  相似文献   
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Perennial ryegrass starts growing when soil temperatures reach 5.5°C for five consecutive days; applying N fertilizer before this risks environmental losses. To test whether daffodil flowering signified when to apply N fertilizer, farmers volunteered to take part in a citizen science study. The PROSOIL project used a “citizen science”, participatory approach to create farmer‐informed science, aiming to increase awareness of the importance of soil health. In 2014, over 300 farmers completed a “How do you manage your soil” survey. The survey included a question on the use of daffodils (Narcissus spp.) to indicate the best time to apply the first nitrogen fertilizer of the season, based on anecdotal feedback from farmers involved in the PROSOIL project. The survey recorded 7% of farmers based their first fertilizer application on when daffodils flowered. To increase farmer awareness of soil temperatures, we provided them with soil thermometers, held workshops and hosted interactive stands at agricultural events in 2014. In autumn 2014, farmers planted daffodil bulbs of the same variety, across Wales, and monitored soil temperatures. Farmers returned postcards once their daffodils were in flower, noting the soil temperature. An assessment of whether daffodil flowering date could indicate when to apply N fertilizer was made. Overall, in spring 2015, daffodils flowered when soil temperature was 6.4(±0.35)°C, suggesting daffodil flowering date is a more reliable indicator for fertilizer application, than first hypothesized. Findings show a scientific validation of local knowledge, regarding the use of daffodils to indicate the “not‐before” date for the first N fertilizer application.  相似文献   
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The mammalian sperm membrane undergoes cholesterol efflux during maturation and fertilization. Although ATP‐binding cassette (ABC) transporters are known to transport cholesterol through cell membranes in other organs, their presence in canine testis, epididymis and sperm has not been proven to date. Hence, the aim of the present study was to localize the ABC transporters ABCA1 and ABCG1 in canine testicular and epididymidal tissue as well as in spermatozoa membranes. To this end, semen samples from 12 dogs as well as testicles and epididymides of four young and healthy dogs were prepared for immunohistochemistry, respectively. Capacitation and acrosome reaction (AR) were induced in aliquots of the semen samples before immunostaining to assess changes in the expression of ABCA1 and ABCG1. Evaluation by confocal microscopy revealed the presence of both ABCA1 and ABCG1 in canine testicles and of ABCA1 in the epididymides. In spermatozoa, only ABCA1 immunoreactivity was detected, mainly in the region of the acrosome and midpiece. After induction of capacitation, ABCA1 signal persisted in the acrosome but disappeared after AR, indicating a loss of ABCA1 with the loss of the acrosome. We conclude that ABCA1 and ABCG1 are expressed in canine testis, whereas only ABCA1 is expressed in epididymis and spermatozoa membrane, both transporters probably contributing to the regulation of membrane cholesterol content.  相似文献   
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The Australian ruminant livestock industries are faced with the need to control parasitic infectious diseases that can seriously impact the health of animals. However, increasing levels of resistance to insecticides, anthelmintics and acaricides are substantially reducing the ability to control some of these parasites. Here we review the current situation with regard to chemical resistances in parasites across the various sectors of the Australian ruminant livestock industries and assess the level of threat that these resistances pose to the sustainability of these sectors in the short to long terms. We also look at the extent to which testing for resistance occurs across the various industry sectors, and hence how well-informed these sectors are of the extent of chemical resistance. We examine on-farm management practices, breeding of parasite-resistant animals, and non-chemical therapeutics that may act as short to long term means to reduce the current reliance on chemicals for parasite control. Finally, we look at the balance between the prevalence and magnitude of current resistances and the availability and adoption rates of management, breeding and therapeutic alternatives in order to assess the parasite control outlook for the various industry sectors.  相似文献   
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The yield and chemical composition of thirteen Lotus corniculatus varieties and one Lotus uliginosus variety, when grown and ensiled in the UK, were investigated. Replicate plots of each variety were established in a randomized block design. Dry‐matter (DM) yield was measured over two harvest years. At cuts 1 and 2 of the first harvest year, 1 kg of each variety was ensiled and sub‐sampled for chemical analysis. At cut 2 of the second harvest year, sub‐samples of forage were analysed for condensed tannins. Two L. corniculatus varieties, Oberhaunstaedter and Lotar, had higher DM yields (with Oberhaunstaedter having the highest DM yield at cut 3) in both harvest years compared with other varieties (P < 0·001). Chemical analyses showed differences among silages of varieties of L. corniculatus (P < 0·001) and that the ammonia‐N concentration of L. uliginosus silage was higher than that of L. corniculatus (P < 0·001), despite its lactic acid concentrations being within the range observed for L. corniculatus (17 g kg?1 DM vs. 13–19 g kg?1 DM). Differences (P < 0·001) in HCl/Butanol test absorbance units were found among varieties of L. corniculatus, indicating possible differences in concentrations of condensed tannins. Overall, the variety Oberhaunstaedter was found to be the most suitable variety for silage production. Based on its agronomic performance, L. corniculatus does not compare well with other legumes such as red clover.  相似文献   
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AIM: To investigate the nature of a neurological disease in Wiltshire sheep.

METHODS: Three affected lambs were examined, humanely killed and necropsied. Selected neurological tissues were examined by light and electron microscopy.

RESULTS: Primary neurological lesions were confined to the cerebellum and were characterised by loss of Purkinje cells and the presence of large hypertrophied dendrites of surviving Purkinje cells. These contained stacks of smooth endoplasmic reticulum. There was hyperplasia and cell swelling of Bergmann glia. Mild Wallerian-type degeneration affected white matter in the cerebellum and spinal cord.

CONCLUSION: The cerebellar lesions were of a degenerative and reactive rather than hypoplastic nature. These, and the history, suggest a genetic cause with putative inheritance as an autosomal recessive trait. Accordingly, the disorder is described as a cerebellar abiotrophy.  相似文献   
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