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A laboratory study was conducted in order to explain successive extractions of reserved potassium by using concentrated HCI under reflux for 13 surface and sub-surface soil samples representing three chosen pedons of soil series （472 ECC Bashika, 463 EKC Rabeaa and 461 ECC Talafar） locations of Nineveh Province in Northern of Iraq. The aim is to investigate role of clay minerals on release of reserved potassium from soils. Results showed that dominated clay minerals were （smectite 〉 illite 〉 kaolinite 〉 chlorite） for both 472 ECC and 461 ECC soil series and were （illite 〉 smectite 〉 kaolinite 〉 chlorite） for 463 EKC soil series. Also results appeared that exchangeable K＋ released values were 2,483-4,575 mg/kg at 461 ECC and 463 EKC soil series, respectively; non-exchangeable phase ranged from 752-1,390 mg/kg at 461 ECC and 472 ECC soil series, respectively. Soil fertility was evaluated according to its K＋ mica release referring for high K＋ release content with range between 3,324-5,516 mg/kg at 461 ECC and 463 EKC soil series, but rate ofK~ released was very law according to parabolic diffusion me,del with range from 195-359 mg/kg at latter soil series respectively. Results reflect effect of clay minerals on potassium release as amount and rates in arid and semi-arid regions. It can be concluded the study soils have a high amount of potassium content with low rate release of it, in turn these soil need particular management. 相似文献
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A. Siah C. Deweer F. Duyme J. Sanssené R. Durand P. Halama P. Reignault 《Plant pathology》2010,59(4):661-670
The association of the cell wall degrading enzyme endo‐beta‐1,4‐xylanase (EC 3.2.1.8) with pathogenicity of Mycosphaerella graminicola was examined in planta. The enzyme production of two M. graminicola isolates (T0372 and T0491), as well as their ability to infect seedlings of susceptible wheat cv. Scorpion, was first compared. No significant difference was found between the two isolates regarding spore germination rates, mycelial growth on the leaf surface or direct and stomatal penetrations. However, restricted hyphal growth was observed inside leaves inoculated with T0372, whereas successful mesophyll colonization with a strong intercellular fungal growth was found in leaves infected with T0491. Likewise, T0372 was unable to induce lesions bearing pycnidia and to produce endo‐beta‐1,4‐xylanase activity until 22 days post‐inoculation (d.p.i.). On the other hand, significant high increases of both diseased leaf area bearing pycnidia and endo‐beta‐1,4‐xylanase activity were observed between 16 and 22 d.p.i. for T0491 (r = 0·98). The investigation of 24 additional isolates, including the IPO323 and IPO94269 reference isolates, highlighted a strong correlation between endo‐beta‐1,4‐xylanase activity and disease development levels (r = 0·94). This study demonstrates that differences in pathogenicity in M. graminicola are not linked to events on the leaf surface or to frequency of leaf penetration, but to the ability of the fungus to colonize the mesophyll and to produce the cell wall degrading enzyme endo‐1,4‐beta‐xylanase during the necrotrophic phase. 相似文献
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We tested here five distinct in vitro screening methods assessing Mycosphaerella graminicola resistance to strobilurin fungicides: (i) spotting on agar medium, (ii) spore germination on agar medium, (iii) growth in microplates without and (iv) with the addition of Alamar blue and (v) screening of the cytochrome b substitution conferring resistance G143A. The assays were performed by assessing resistance to azoxystrobin of 32 French M. graminicola strains and the two reference strains IPO323 and IPO94269 from the Netherlands. The microplate Alamar blue assay displayed high standard deviations from all growth averages, hence reporting the strong lack of reliability of this method. As expected, the two reference strains were found sensitive with all other methods. In agreement with the disruptive resistance of M. graminicola to single-site fungicides, the half maximal inhibitory concentration (IC50) values of sensitive and resistant strains displayed a bimodal distribution pattern with each type of assay. All strains carrying G143 or A143 alleles were found sensitive or resistant respectively with each bioassay. Although the absolute IC50 value for each strain varied among assays, the ranking of strains according to their IC50 values was overall conserved with all assays. Finally, the frequency of strobilurin resistant haplotypes within France was investigated by screening G143A in 82 strains isolated in 2005 year from 12 localities. Results showed a marked gradual decrease in resistance distribution from 70% in northern to 30% and 0% in central and southern France, respectively. The accuracy of each method as well as the widespread and incidence of strobilurin resistance within the French population of M. graminicola have been discussed. 相似文献
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Samir Mohammad Ali Alredaisy 《干旱区科学》2011,3(3):174-183
The climatic, geomorphic, hydrologic and aquifer characteristics of the torrential Gash River across mountainous areas, in far eastern Sudan, were analyzed in order to mitigate its recurring catastrophic impacts. Hydrologic and climatic data and interpretation of Gash River satellite images were from relevant research works carried out in Gash Basin from 1985 to 2008. The results indicated that the total catchment area of Gash River is about 21,000 km2, and the basin area is 31,000 km2. The total length of Gash River is 450 km and the average slope is 200 cm/km. The width of the catchment varies from 30 m to 90 m and that of the basin is from 100 m to 800 m, and the course of the river is varied. During the period of 1980?2008, the mean annual rainfall was 250 mm and the mean annual discharge was 6.8 ×10~8 m~3, and the flooding is approximately one time per 5 years. Torrential floods measured at Kassala town since 1907 showed that an absolute maximum discharge was 876 m3/s and a mean maximum discharge was 365 m3/s. These characteristics differ widely between catchment and basin areas. The author proposes that, by analyzing the climatic, geomorphic, hydrologic and aquifer characteristics of the Gash River and considering the role of community, a concise database could be provided to formulate the aquifer, geomorphic, hydrologic and climatic (AQUIGEOHYCLIM) regional approach to mitigate Gash River recurring catastrophic impacts. 相似文献
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Cultivar‐dependent partial resistance and associated defence mechanisms in wheat against Zymoseptoria tritici 
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下载免费PDF全文 M. E. Ors B. Randoux S. Selim A. Siah G. Couleaud C. Maumené K. Sahmer P. Halama P. Reignault 《Plant pathology》2018,67(3):561-572
Septoria tritici blotch caused by the fungus Zymoseptoria tritici is one of the most devastating foliar diseases of wheat. Knowledge regarding mechanisms involved in resistance against this disease is required to breed durable resistances. This study compared the expression of defence and pathogenicity determinants in three cultivars in semicontrolled culture conditions. The most susceptible cultivar, Alixan, presented higher necrosis and pycnidia density levels than Altigo, the most resistant one. In Premio, a moderately resistant cultivar, necrosis developed as in Alixan, while pycnidia developed as in Altigo. In noninfectious conditions, genes coding for PR1 (pr1), glucanase (gluc) and allene oxide synthase (aos) were constitutively expressed at a higher level in both Altigo and Premio than in Alixan, while chitinase2 (chit2), phenylalanine ammonia‐lyase (pal), peroxidase (pox2) and oxalate oxidase (oxo) were expressed at a higher level in Premio only. Except for aos, all genes were induced in Alixan during the first steps of the symptomless infection phase. Only pox2, oxo, gluc and pal genes in Altigo and pal, chs and lox genes in Premio were up‐regulated at some time points. Basal cultivar‐dependent resistance against Z. tritici could therefore be explained by various gene expression patterns rather than high expression levels of given genes. During the necrotrophic phase, Z. tritici cell wall‐degrading enzyme activity levels were lower in Altigo and Premio than in Alixan, and were associated more with pycnidia than with necrosis. Similar tissue colonization occurred in the three cultivars, suggesting an inhibition of the switch to the necrotrophic lifestyle in Altigo. 相似文献
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Beibei Jia Marina K. V. C. Delphino Babafela Awosile Tim Hewison Patrick Whittaker Diane Morrison Mykolas Kamaitis Ahmed Siah Barry Milligan Stewart C. Johnson Ian A. Gardner 《Journal of fish diseases》2020,43(2):153-175
Wild Pacific salmonids (WPS) are economically and culturally important to the Pacific North region. Most recently, some populations of WPS have been in decline. Of hypothesized factors contributing to the decline, infectious agents have been postulated to increase the risk of mortality in Pacific salmon. We present a literature review of both published journal and unpublished data to describe the distribution of infectious agents reported in wild Pacific salmonid populations in British Columbia (BC), Canada. We targeted 10 infectious agents, considered to potentially cause severe economic losses in Atlantic salmon or be of conservation concern for wild salmon in BC. The findings indicated a low frequency of infectious hematopoietic necrosis virus, piscine orthoreovirus, viral haemorrhagic septicaemia virus, Aeromonas salmonicida, Renibacterium salmoninarum, Piscirickettsia salmonis and other Rickettsia-like organisms, Yersinia ruckeri, Tenacibaculum maritimum and Moritella viscosa. No positive results were reported for infestations with Paramoeba perurans in peer-reviewed papers and the DFO Fish Pathology Program database. This review synthesizes existing information, as well as gaps therein, that can support the design and implementation of a long-term surveillance programme of infectious agents in wild salmonids in BC. 相似文献
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Detection of eastern equine encephalomyelitis virus antigen in equine brain tissue by enzyme-linked immunosorbent assay 总被引：1，自引：0，他引：1
T W Scott J G Olson B P All E P Gibbs 《American journal of veterinary research》1988,49(10):1716-1718
Sensitivity and specificity of an antigen-capture ELISA vs virus isolation in cell culture were evaluated for the detection of eastern equine encephalomyelitis (EEE) virus in the brain tissue of naturally infected equids. Brain specimens from 16 equids with neurologic disease were examined by ELISA and by inoculation onto baby hamster kidney cell cultures. Of 10 brain samples from which virus was isolated in the cell culture bioassay, all were correctly identified as containing EEE virus antigen by ELISA. None of the remaining 6 specimens, without detectable infectious EEE virus, contained detectable antigen. Sensitivity and specificity of the ELISA were 100% with no false-positive or false-negative results. The antigen-capture ELISA was a rapid, sensitive, specific, and simple alternative to a traditional bioassay for the detection of EEE virus. 相似文献