牦牛发情周期中卵巢卵泡发育状况的组织学观察     

蒙学莲 崔燕余四九  刘学荣 《中国兽医科技》2006,36(1):57-61

运用组织学技术对36头处于发情周期的健康成年母牦牛卵巢卵泡的结构与发育状况进行了观察。结果表明，牦牛发情周期中卵巢卵泡发育的组织结构与其他牛基本相似。每对卵巢中原始卵泡数和生长卵泡数在发情前期、发情期、发情后期和发情间期之间差异不显著（P〉0．05）；发情前期的囊状卵泡数与发情期、发情后期和发情间期之间差异不显著（P〉0．05），发情期和发情后期均与发情间期差异显著（P〈0．05）。闭锁生长卵泡数在4个时期之间差异不显著（P〉0．05）；发情期的闭锁囊状卵泡数与其他3期之间差异极显著（P〈0．01），发情前期和发情间期均与发情后期差异显著（P〈0．05）。各级卵泡的闭锁形式各有特点。在生长卵泡、囊状卵泡及相应闭锁卵泡的卵泡膜中均见到了胶原纤维和网状纤维。  相似文献   

雏鸭胸腺生长指标的动态观测及组织学观察     

方静  崔恒敏彭西 何敏 《中国兽医科技》2006,36(5):401-405

通过对1～49日龄雏鸭胸腺发育的组织学观察及胸腺重量、胸腺指数和外周血T淋巴细胞α-醋酸萘酯酶阳性率（ANAE^＋）的测定，研究了雏鸭胸腺的生长及组织发育规律。结果显示，胸腺重量随日龄增长逐渐增加；胸腺指数在28日龄时达最高；1～28日龄时外周血T淋巴细胞ANAE^＋上升幅度较大；21～28日龄时胸腺小叶数量增多，并显著增大；28日龄时皮质部主要为小淋巴细胞；胸腺小体有3种形态。表明，雏鸭在1～14日龄时胸腺生长较缓慢，21～28日龄时生长十分迅速，28日龄时胸腺中大部分T淋巴细胞已发育成熟，35～49日龄时胸腺发育已趋于稳定；胸腺小体是具有高度活性的结构，与清除退变上皮有关。  相似文献   

高铜对雏鸡器官组织病理损害的研究     

彭西朱奎成  徐之勇邓俊良 崔恒敏 《中国兽医科技》2006,36(8):659-664

将420只1日龄艾维菌健雏，随机分为7组，分别饲以含铜量11mg／kg的基础日粮和含铜量分别为100、200、300、400、500和600mg／kg的6种高铜日粮6周，研究高铜对雏鸡的病理损害。结果显示，日粮含铜100和200mg／kg对雏鸡有促生长作用。日粮铜水平达300mg／kg以上时，对雏鸡器官组织造成不同程度的病理损伤，表现为肌胃角质层增厚呈黄褐色；肝呈浅黄色或深褐色，肝细胞脂肪变性；消化道上皮细胞变性坏死；免疫器官和肾、心脏、胰腺、大脑等器官的实质细胞肿胀；淋巴免疫器官体积缩小，淋巴细胞数量减少。超微结构观察发现，肝细胞胞浆和胞核内见有数量不等、大小不一的高电子密度的沉积物；凋亡的淋巴细胞染色质呈花瓣样、半月形或环形，聚集在核膜下。表明，肝、消化道和免疫器官是高铜损害的主要靶器官。  相似文献   

鸡新城疫病毒的分离与鉴定     

刘栋  廉中华  崔言顺  李建亮  朱玉成 《家畜生态》2006,(6)

从山东某鸡场的产蛋下降而无其他典型新城疫(Newcastle Disease)症状的高抗体蛋鸡群中分离到一株病毒。经过试验鉴定,该分离株具有血凝性,且可被ND标准阳性血清所抑制和中和,不能被AI(H5亚型与H9亚型)标准阳性血清抑制;该病毒的最小致死量病毒致死鸡胚的平均时间(h)为50.4 h,1日龄SPF鸡脑内接种分离病毒致病指数(ICPI)为1.85,6周龄SPF鸡静脉接种致病指数(IVPI)为2.42,回归试验鸡出现了新城疫症状和病变,经外源病毒检测及回归试验该病毒株被确定为新城疫病毒,并属强毒型,命名为ShD-5-04。  相似文献   

一例猪附红细胞体与弓形体混合感染的诊治     

崔双保  和彩红 《中国兽药杂志》2006,40(3):58-59

附红细胞体病是由立克次氏体引起的一种散在的热性、溶血性入畜共患传染病，病猪以急性黄疸性贫血和发热为特征。弓形体病是由刚地弓形虫在多种动物和入的有核细胞内寄生引起的一种入畜共患的原虫病。本文通过对某猪场发生猪附红细胞体与弓形体混合感染的诊治，从发病情况、临床症状、剖检变化、实验室诊断、治疗等方面对该病进行了阐述。  相似文献   

6种多年生牧草对幼龄果园的影响     

张志刚  崔同华  谷艳蓉 《草原与草坪》2007,(5):57-59

通过在幼龄果园间作6种多年生牧草,结果表明,幼龄果园土壤含水量增加,杂草生长得到抑制,6种多年生牧草品种在果园间作的适宜度大小顺序为:白三叶(Haifa)>紫花苜蓿(Empress 2000)>杂三叶(Aurora)>沙打旺(普通)>鸭茅(Amba)>多年生黑麦草(Toya)。  相似文献   

氮肥对保水剂吸水保肥性能的影响   总被引：7，自引：0，他引：7  

苟春林  杜建军  曲东  崔英德  毋永龙 《干旱地区农业研究》2006,24(6):78-84

测定保水剂在不同氮肥(尿素、碳酸氢铵、硝酸铵、硫酸铵、氯化铵)溶液中的吸水倍率和对肥料养分的吸持量,分析不同氮肥对保水剂吸水、保肥性能的影响。结果表明:保水剂在各种氮肥溶液中吸水倍率显著下降,并随肥料浓度的增加下降幅度增加;受影响程度聚丙烯酸钠盐型保水剂大于聚丙烯酰胺-丙烯酸盐型保水剂;不同氮肥对保水剂吸水倍率的影响按尿素、碳酸氢铵、硝酸铵、硫酸铵、氯化铵顺序递增。保水剂在大量吸水的同时,也对溶液中的肥料分子或者离子有吸持作用,吸持量均随肥料浓度的增加而增加;保水剂和肥料品种不同,吸持量也不同;除尿素外,保水剂对肥料的吸持率随肥料浓度的增加而降低,最高浓度下,吸持率按硝酸铵、碳酸氢铵、氯化铵、硫酸铵、尿素次序依次增大。  相似文献   

西宁市朝阳地区斜面草坪的建植与品质评定     

宋桂玲  崔凯 《草业与畜牧》2007,(10):23-27

：通过对青海西宁朝阳区高速公路网斜面护坡不同品种草坪的建植，总结斜面草坪在西宁地区的建植技术，并根据不同类型草坪评价指标的权重，对草坪的密度、质地、叶色、均一性、覆盖度、越冬力、草坪强度等7项指标进行了测定，结果表明：在西宁地区用紫羊茅＋高羊茅＋多年生黑麦草直播和草地早熟禾直播建植斜面草坪，在观赏和水土保持性能方面表现突出，可在西宁地区斜坡绿化地带推广。  相似文献   

Prokaryotic Expression and Polyclonal Antibody Preparation of σC Protein of Novel Duck Reovirus DH13 Strain     

LI Wenjun  HUANG Huilan  YANG Huihu  XIE Zimin  CUI Huiyi  HUANG Shujian  ZHANG Xuelian 《中国畜牧兽医》2007,47(9):2953-2959

The aims of the experiment was to optimize the prokaryotic expression system of σC protein,prepare polyclonal antibody against σC protein of novel duck reovirus (NDRV),and evaluate the titer of the antibody.The σC gene of NDRV-DH13 strain was amplified by RT-PCR,ligated into pET-30a(+) and pET-32a(+) expression vector,constructed prokaryotic expression plasmid,which were transformed into E.coli BL21(DE3) and the expression of the σC protein were induced by IPTG.The proteins expression were analyzed by SDS-PAGE.The recombinant protein without His tag was purified by digestion,and the recombinant protein with His-tagged was purified by Ni-NTA column.Then the polyclonal antibody was obtained from rabbits which had been immunized by the purified protein without His tag.Anti-His-labeled mAb and NDRV-σC positive serum were used as primary antibodies to evaluate antibodies specificity,the antibodies titer was detected by indirect ELISA (iELISA).SDS-PAGE results showed that the molecular weight of the expression on recombinant proteins were 34 and 37 ku respectively,the proteins were highly expression.Western blotting showed that they had the specific reaction and the prepared antibodies had higher affinity with σC protein,the titer were about 1:25 600 by iELISA detection.This study successfully constructed and optimized the prokaryotic expression system of the polyclonal antibody against σC protein,laid a foundation for the further study of σC protein and the research of genetically engineered vaccine.  相似文献   

Research Progress of Canine Coronavirus and Feline Coronavirus     

LI Shaohan  ZHANG Guangzhi  CUI Shangjin  QIN Tong 《中国畜牧兽医》2007,47(12):4059-4068

Canine coronavirus (CCoV) and feline coronavirus (FCoV) belong to α-genus coronavirus of coronavirus family,porcine transmissible gastroenteritis virus (TGEV),porcine epidemic diarrhea virus (PEDV) also belong to the same genus.Genetic evolution analysis showed that different genotype of the virus could produce new variant strains through gene recombination,which caused great obstacles to the diagnosis and control of the disease.β-genus coronaviruses include bovine coronavirus (BCoV),canine respiratory coronavirus (CRCoV) and severe acute respiratory syndrome coronavirus (SARS-CoV).Among them,CRCoV has the highest homology with BCoV,but there are great differences in genomic structure,pathogenic mechanism and infection symptoms between this kind of coronavirus and α-coronavirus.CCoV and FCoV are widely spreading around the world,characterized by high morbidity and low mortality.Due to the characteristics of RNA virus and the influence of environmental selection pressure,the viruses continue to mutate and evolve,and new virulent strains appear one after another.The virulence of feline infectious peritonitis virus (FIPV) is greatly enhanced,some specific point mutations in the virus genome change the cellular tropism against the host.The pathogenesis of the virus mainly depends on the antibody-dependent enhancement (ADE) induced by virus infection.The epidemiological investigation and prevention and control of CCoV and FCoV should not only rely on the single factor of vaccine immunity,but also comprehensively consider the virulence of the virus,environmental conditions,pet self-immune resistance, and so on.The identification of CCoV and FCoV should be based on clinical symptoms,combined with routine hematological examination,serum biochemical examination and laboratory diagnosis techniques to prevent false positive and false negative results.  相似文献