The nutritional value of low lathyrogenic Lathyrus (Lathyrus sativus) for growing chicks     

R. G. Rotter  R. R. Marquardt  C. G. Campbell 《British poultry science》1991,32(5):1055-1067

1. The feeding value of new low β‐N‐oxalyl‐amino‐L‐alanine (BOAA) lines of Lathyrus sativus (lathyrus) and the benefits of dehulling the seed or of pre‐adapting chicks to lathyrus‐based diets were examined in several experiments.

2. Chicks fed on diets containing 400 g/kg of the low (1.3 g BOAA/kg seed) and medium (2.2 g BOAA/kg seed) BOAA lines did not differ (P>0.05) in weight gain (WG) or in apparent fat and protein digestibilities compared to birds fed on a wheat‐based diet.

3. Consumption of 600 g low BOAA lathyrus/kg diet caused a slight, but significant (P< 0.05), decrease in WG. Food efficiency decreased as the amount of dietary lathyrus was increased, suggesting that lathyrus may contain an antinutritive factor(s) other than BOAA.

4. Removal of the hull (70 g hull/kg seed) from the seed did not affect chick performance (P> 0.05).

5. Pre‐adapting chicks for 7 d to diets containing up to 600 g medium line lathyrus/kg diet did not reduce the detrimental effects of the lathyrus.

6. Although dehulling and pre‐adaptation of chicks to lathyrus were not beneficial, the low and medium lines of lathyrus tested show potential for use in chick diets up to at least 400 g lathyrus/kg diet.  相似文献   

Male pseudohermaphroditism of the testicular feminization type in a heifer   总被引：1，自引：1，他引：0       下载免费PDF全文

Peter AT  Scheidt AB  Campbell JW  Hahn KA 《The Canadian veterinary journal. La revue veterinaire canadienne》1993,34(5):304-305

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A survey of the selenium status of beef cows in Alberta.   总被引：1，自引：1，他引：0  

J R Campbell  G K Jim  C W Booker    P T Guichon 《The Canadian veterinary journal. La revue veterinaire canadienne》1995,36(11):698-702

An epidemiological survey was conducted in Alberta to measure the selenium status in blood of beef cows during the fall and to determine the risk of selenium deficiency among specific geographic regions of Alberta. Three census divisions of Alberta based on the Statistics Canada Census of Agriculture were chosen as the study areas for the project. Soils and plants in area A (Edmonton area) and area B (Calgary area) were known to be deficient in selenium, while soils and plants in area C (southeast corner of Alberta) were known to have adequate levels of selenium. Blood samples were collected from 335 cows on 29 farms from the 3 study areas. These samples were collected from cows that had recently been removed from pasture in October and November 1992. Answers to a short questionnaire pertaining to various herd characteristics and management practices were also obtained for each herd. The average value of selenium for all cows sampled was 2.20 mumol/L. The average value of selenium of cows in areas A and B was 1.93 mumol/L. The average value of selenium of cows in area C was significantly (P < 0.05) higher at 2.70 mumol/L. Nine percent of the cows in the study were considered marginal or deficient in selenium (< 1.27 mumol/L selenium). Herds located in area C, herds that were provided with supplemental feed on pasture, and herds that were pregnancy checked had higher average herd selenium values than did other herds. Cow-calf producers located in areas with selenium-deficient soils should pay particular attention to selenium supplementation for their cows. Some of the negative "geographic" effects on selenium values can be overcome by more progressive management practices.  相似文献   

Study on adherent precursors from human cord blood     

AN Xiao-hui  CAI Guo-ping 《园艺学报》2003,19(11):1449-1452

AIM: To confirm the existence of the endothelial progenitor cells in human cord blood and to study its differentiation and development process. METHODS: The mononuclear cells in human cord blood were isolated using lymphocyte separation solution. Then the mononuclear cells were cltured in MCDB131 containing 20% fetal bovine serum. The effects of 5 μmol/L dexamethasone, the extract from bovine brain, insulin and hypoxanine on the proliferation and differentiation of the adherent cells were observed. The morphology of the adherent cells were examined twice daily by inverted phase contrast microscope. CD34 and CD14 expression were determined by FACS. Immunohistochemistry was used to confirm the expression of factor Ⅷ. RESULTS: The proliferative endothelial progenitor cells existed within the CD34^-adherent mononuclear cells of human cord blood. Dexamethasone and hypoxanine decreased the number of spindle-shaped cells and caudated cells. Bovine brain extract, insulin and FCS enhanced the number of spindle-shaped cells and caudated cells. CONCLUSION: The existence of endothelial progenitor cells within the CD34 - adherent monouclear cells of the human cord blood was observed and these cells were able to differentiate into endothelial-like cells in vitro.  相似文献   

Leaf, floret and seed infection of wheat by Pyrenophora semeniperda     

M. A. Campbell  R. W. Medd †‡ 《Plant pathology》2003,52(4):437-447

Infection processes of Pyrenophora semeniperda on seedling and adult wheat leaves and wheat ears were investigated. Almost 100% germination of conidia occurred on seedling leaves, compared with 20–30% on adult leaves. Appressoria formed over the anticlinal epidermal cell walls and haloes always accompanied infection. Sometimes papillae formed within the leaves as a resistance mechanism. Infection hyphae ramified through the intercellular spaces of the mesophyll resulting in cellular disruption. The infection processes on floral tissues were similar to those observed on leaves; however, no infection occurred on anther, stigmatic or stylar tissues. Infection of ovarian tissue occurred both with and without appressoria formation. Hyphae grew mainly in the epidermal layers and appeared unable to breach the integumental layer as no growth was observed in endosperm or embryo tissues. The optimum dew period temperature for conidial germination was 23·6°C, compared with 19·9°C for lesion development, 20·4°C for the production of infection structures on seedling leaves and 23·7°C for floret infection. Leaf disease development occurred in a logistic manner in response to dew period, with maximum infection observed after 21 h compared with > 48 h in seeds. An initial dark phase during the dew period was necessary for infection and temperature after the dew period had an effect, with significantly more numerous and larger lesions being formed at 15°C compared with 30°C. Seedling leaves were found to be more susceptible than older leaves, under both field and controlled environment conditions. Infection of wheat seeds following inoculation of ears, or after harvest burial of inoculated disease-free seeds, was demonstrated. In the latter, 3-week-old seedlings were slightly stunted, whereas older plants were unaffected. The apparent unimportance of this plant pathogen as a cause of leaf disease in relation to its poor adaptation to dew periods and dew period temperature is discussed, along with the importance of its seed borne characteristics.  相似文献   

动物组织中四环素类抗生素残留的ELISA检测研究——土霉素抗体的制备   总被引：3，自引：0，他引：3  

安清聪  张曦  陈克嶙 《畜牧与兽医》2004,36(9):8-10

四环素类抗生素属小分子半抗原物质 ,必须与大分子物质结合才能产生免疫原性。本试验采用重氮化法合成土霉素 牛血清白蛋白 (BSA OTC)及土霉素 鼠血清白蛋白 (RSA OTC)人工抗原 ,用紫外扫描对其进行鉴定。以BSA OTC免疫家兔制备抗血清 ,以RSA OTC为包被抗原 ,采用双向免疫扩散法和ELISA方法对抗血清进行鉴定。结果表明用重氮化法合成的人工抗原对四环素类药物有特异的抗原抗体反应 ,抗血清效价达 1 2 8× 1 0 5,完全能够满足四环素类药物残留的ELISA检测要求  相似文献   

土壤易氧化有机碳与溶解性有机碳对荒漠草地沙漠化过程中土壤碳库变异的表征     

阎欣  刘任涛  安慧 《草业学报》2018,27(11):15-25

以干旱、半干旱地区荒漠草地不同沙漠化阶段土壤为研究对象,研究不同沙漠化阶段土壤易氧化有机碳与溶解性有机碳含量和分配比例变异规律,分析不同沙漠化阶段土壤碳库指数变化,探讨荒漠草地沙漠化过程中土壤易氧化有机碳与溶解性有机碳对土壤碳库的表征作用。结果表明,随着荒漠草地沙漠化程度的加剧,易氧化有机碳含量表现出一定的波动性,但整体呈下降趋势,溶解性有机碳含量呈线性下降。易氧化有机碳和溶解性有机碳分配比例均在荒漠草地达到最大值,在半固定沙地达到最小值。利用易氧化有机碳含量计算的土壤碳库管理指数随荒漠草地沙漠化程度增加表现出先下降后上升趋势,利用溶解性有机碳含量计算的土壤碳库管理指数呈线性下降趋势。易氧化有机碳、溶解性有机碳含量与土壤有机碳均显著正相关,但溶解性有机碳的相关系数略高于易氧化有机碳。通过比较活度敏感指数和碳库管理指数敏感指数发现,荒漠草地逆向演替至固定沙地过程中,易氧化有机碳的各项敏感指数均高于溶解性有机碳。固定沙地演替至流动沙地过程中,溶解性有机碳的各项敏感指数均高于易氧化有机碳,表明易氧化有机碳能够较好地表征荒漠草地沙漠化前期土壤碳库变化,而荒漠草地沙漠化中期和后期利用溶解性有机碳表征效果更好。  相似文献   

Effect of Human Menopausal Gonadotropin,17β-estradiol and Epidermal Growth Factor on the Quality of in vitro Maturation Bovine Oocytes     

TANG Shuang  AN Wen-zhong  JIANG Xin-long  LI Xiang-chen 《中国畜牧兽医》2016,43(6):1585-1590

For optimizing in vitro maturation system of bovine oocytes,we firstly examined the influence of four different hormonal regimes(FSH+LH,HMG,FSH+LH+E₂ and HMG+E₂) on oocyte maturation rates.Then we studied the effects of epidermal growth factor (EGF) in the above defined medium on bovine oocyte maturation,in vitro development and quality of parthenogenetic embryos.The cell apoptotic index of parthenogenetic blastocysts was detected by TUNEL.No significant difference was observed in maturation rates in four groups supplemented with different hormones.However,human menopausal gonadotropin (HMG) provided steady maturation results in replicates.Maturation of oocytes was promoted by supplementation with 17β-estradiol (E₂).Combination of HMG and E₂ gave rise to steady and efficient mature results.The presence of EGF at 30 ng/mL concentration significantly increased maturation rate and blastocyst rate and reduced apoptotic cells in parthenogenetic blastocysts.Therefore,the optimal oocyte maturation solution could be supplemented with 0.075 IU/mL HMG,1 μg/mL E₂ and 30 ng/mL EGF.  相似文献   

UCP1基因变异与绵羊生长及胴体性状的关联分析     

安清明  周辉通  王星  吴震洋  罗玉柱  Jon G. Hickford 《中国畜牧兽医》2019,46(11):3289-3298

试验旨在探讨解耦联蛋白1（UCP1）基因核苷酸变异对绵羊生长性状的性别差异和胴体性状的影响，以期能够筛选出可以提升绵羊生长及胴体性状的核苷酸变异，为提高绵羊相关重要经济性状的分子遗传标记提供材料。以9个绵羊品种为研究对象，用PCR-SSCP方法检测不同性别绵羊中UCP1基因内含子5区和外显子6区变异。利用Minitab 16.0软件中一般线性模型分析内含子5区等位基因与不同性别绵羊生长性状、公羔胴体性状的关联性。结果显示，绵羊UCP1基因内含子5区和外显子6区共检测到8个核苷酸变异，其中位点c.910 G/A突变导致p.Ala304Thr氨基酸变异。生长性状关联分析结果表明，内含子5区等位基因对绵羊生长性状的影响存在性别特异性，母羔中携带等位基因A₁的群体较缺失群体具有较低的初生重（P<0.05），公羔中携带等位基因C₁的群体较缺失群体具有较高的断尾重（P<0.05），未发现其他等位基因与羔羊的生长性状存在性别特异性。胴体性状关联分析结果表明，携带等位基因A₁的群体具有较低后腿瘦肉量、腰部瘦肉量和较高的肩部瘦肉比例（P<0.05），携带等位基因C₁的群体具有较低的后腿瘦肉比例（P<0.05），其他胴体性状均没有发现与等位基因存在显著相关，基因型分析结果与等位基因分析结果一致。结果提示，UCP1基因对绵羊的生长性状影响具有性别特异性，且携带等位基因A₁的公羔群体具有较低的胴体生产性状，为提高公羔胴体生产性能提供了理论依据。  相似文献   

江苏盐城首次记录到彩鹮     

刘彬  薛丹丹  安玉亭  王立波  孙大明  杨禹治  鲁长虎 《野生动物学报》2021,(2):594-595

2020年5月4日在江苏盐城大丰麋鹿国家级自然保护区(120°47′—120°53′E,32°59′—33°03′N)记录到3只彩鹮。栖息地为一处浅滩水塘,3只彩鹮于水中觅食。以往曾宣布彩鹮在国内绝迹,本次记录填补了彩鹮在本地区的分布空白,证实了彩鹮在江苏省仍有分布。这一纪录或许说明本地区栖息地得到了有效的改善。  相似文献