PEG处理对大豆幼苗活力及活性氧代谢的影响   总被引：20，自引：0，他引：20  

顾龚平  吴国荣  陆长梅 《中国油料作物学报》2000,22(2):26-30

采用ＰＥＧ－６０００（聚乙二醇）浸润大豆种子（黑脐９１－１）,能显著提高种子萌发率和幼苗活力,降低吸水萌发时的外渗电导率,其中以２０％ＰＥＧ（Ｗ／Ｗ）处理８ｈ,效果最为显著,在大豆萌发过程,ＰＥＧ处理组的呼吸速率初始低于对照,之后增加迅速,超氧阴离子（Ｏ＾－２）含量显著低于对照组、超氧化物歧化酶（ＳＯＤ）活性和过氧化物酶（ＰＯＤ）的活性均显著高于对照;脂质过氧化产物丙二醛（ＭＤＡ）的积累量明显低于  相似文献   

提高条斑紫菜壳孢子采苗量试验     

龚琪本  马善乐 《河北渔业》2006,(10):30-32,50

条斑紫菜养殖业是我省海洋渔业的支柱产业,是可以在我国北方沿海地区广泛推广的出口创汇产业,发展前景极为广阔.发展紫菜养殖业,苗种是关键.笔者将江苏南北紫菜育苗结果进行对比后认为,南通地区的条斑紫菜育苗的壳孢子采苗时间早(一般为9月18-30日)、壳孢子放散集中、网帘下海及时,但壳孢子集中放散的高峰期只有2～3d,正常采苗量1×667 m2/m2.  相似文献   

Cloning of the full-length coding sequence and expression analysis of Sox9b in guppy <Emphasis Type="Italic">(Poecilia reticulata)</Emphasis>     

Xue-Yan Shen  Jian-Zhou Cui  Qing-Li Gong  Yoshitaka Nagahama 《Fish physiology and biochemistry》2007,33(3):195-202

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Activities of digestive enzymes and histology of digestive system during larval development of devil stinger (Inimicus japonicus)          下载免费PDF全文

Songlin Li  Wen Wen  Xuxiong Huang  Xu Gong  Longfeng Feng  Naisong Chen 《Aquaculture Research》2017,48(10):5394-5401

Devil stinger is a valuable demersal scorpaenid fish while the rearing of stinger larvae still relies on live prey. This study was conducted to illustrate the development of the main digestive enzymes and digestive system during larval development of this species to provide evidence for the application of artificial feeds. Enzymatic and histological assays were conducted from 1 day post hatching (dph) to 36 dph in larvae. The result showed that the selected digestive enzyme activities increased significantly after 15 dph. Specifically, the total trypsin activities increased significantly from 18 dph to 33 dph. The total pepsin and amylase activities increased significantly first and thereafter decreased significantly. The lipase activities followed the similar pattern with trypsin. With regard to the histological study, the stinger larvae open their mouth to first feeding at 3 dph and turned into totally exogenous nutritional stage at 6 dph. In addition, mucous membrane, rich in goblet cells, was widely distributed in oesophagus epithelium at 18 dph. The height and amounts of gastric gland in cardia and main body of the stomach increased gradually with the development of stinger larvae after 15 dph. The intestine length of stinger larvae was short, and goblet cell was abundant in anterior intestine after 12 dph, not the posterior intestine. The ontogeny of liver and pancreas started from newly hatched stage, and the differentiation of liver was prior to pancreas. The above findings would provide evidence for the use of artificial feeds from the larval stage of stinger larvae (at least from 21 dph).  相似文献   

Dietary supplementation of sodium butyrate may benefit growth performance and intestinal function in juvenile grass carp (Ctenopharyngodon idellus)          下载免费PDF全文

Mengmei Liu  Wei Guo  Fan Wu  Qicai Qu  Qingsong Tan  Wangbao Gong 《Aquaculture Research》2017,48(8):4102-4111

A 49‐days feeding trial was conducted to study the effects of sodium butyrate on growth performance, gut morphology of juvenile grass carp. Five isoenergetic and isonitrogenous experimental diets were compounded by the supplementation in the basal diet with gradient sodium butyrate at 0, 500, 1000, 2000, 3000 mg kg^?1 respectively. A total of 375 juvenile grass carp (with initial body weight of 3.8 g) were randomly allocated into five diet treatments, and each treatment has three replicates. The results showed that the specific growth rate (SGR) of SB1000 and SB2000 group was significantly higher (P < 0.05) than that of the other groups. Moreover, the lowest SGR was observed in SB3000 group. Feeding rate and the whole‐body proximate composition including moisture, crude lipid, crude protein and crude ash were not affected by sodium butyrate (P > 0.05). Total superoxide dismutase activities in hepatopancreas in the experimental groups were significantly higher than those in the control group (P < 0.05). Glutathione peroxidase activity in hepatopancreas was significantly upregulated by dietary sodium butyrate level (P < 0.05). However, the activity of total antioxidant capacity and the contents of malondialdehyde were not significantly different among groups. The expression levels of mRNA encoding PepT1 and LAT2 in the foregut both showed a first increasing and then decreasing tendency as dietary sodium butyrate level increased (P < 0.05), and peaked in SB1000 and SB2000 groups respectively. The results indicated that appropriate dietary supplementation of sodium butyrate at 2000 mg kg^?1 could improve the growth, antioxidant ability and intestinal absorption capacity of the juvenile grass carp.  相似文献   

工厂化养鱼中氧气锥的增氧规律   总被引：2，自引：0，他引：2  

陈有光  段登选  陈秀丽  巩俊霞  王妹 《渔业现代化》2009,36(3):26-30

控制养殖水体溶氧是工厂化养鱼水处理主要技术之一.介绍一种工厂化养鱼氧气锥的结构、原理及增氧效果.为了掌握氧气锥增氧规律,对氧气锥进行了氧气流量单因素等差梯度试验.结果表明,在循环水流量为65 m3/h,水温24.5℃,氧气流量为0,1,2,……,10 L/min的条件下,溶氧与氧气流量回归方程为y=0.100 9x2+0.059x+5.958 8(r=0.992 7);根据物料平衡原理,推算出氧气锥的最大氧气利用率为84.56%;在最大氧气利用率的条件下,依照尼罗罗非鱼的耗氧率计算模式,推算出工厂化养鱼系统需配置氧气锥的台数.  相似文献   

人工养殖湖泊花(鱼骨)卵巢周年变化与性成熟年龄的研究     

龚世园 《淡水渔业》1992,(4):20-23

对花(鱼骨)作组织学研究表明,人工养殖湖泊中花(鱼骨)的卵巢发育可划分为六期,花(鱼骨)的性成熟年龄为一冬龄。  相似文献   

基于被动声学技术的海洋生物测量研究进展   总被引：1，自引：0，他引：1  

陈功  王平波  庞韬  于海平  杜玉华  李耀波 《渔业现代化》2014,(6)

综述了开展基于被动声学技术实现海洋生物测量的重要性;介绍了国内外被动声学技术用于海洋鱼类测量的研究背景、发展现状、存在的问题和发展趋势。列举了常用的被动测量设备以及被动声学数字信号处理方法,包括数据采集、预处理、端点检测、特征提取和识别技术;分析了采用上述测量技术可以研究海洋鱼类的栖息地、生活习性以及人类活动对鱼类的影响。通过列举石首鱼和鳕鱼的发声研究,提出今后被动声学技术研究的重点应集中于各种被动鱼目标特征库的建立和完善,鱼类、海豚等海洋生物的被动声辐射机理的分析,具有代表性的声特征参数以及鱼类发声和特殊行为之间的关联的研究。  相似文献   

圆斑星鲽染色体与多种显带的形态特征分析     

王妍妍  柳学周  刘新富  徐永江  孙中之  兰功刚 《水产学报》2011,35(11):1607-1616

采用体内注射植物血细胞凝集素(PHA)和秋水仙素法制备圆斑星鲽染色体,研究其染色体C-带、Ag-NORs带及G-带的显带特征.结果发现,圆斑星鲽具有46条染色体,核型为2n =46t,染色体臂数为NF =46,所检个体全部为二倍体,未发现存在异型性染色体和随体染色体的现象.带型研究表明,C-带特征为46条染色体均有大小不一的C-带,其中第22对染色体整体呈阳性深染,第19对染色体具有端部C-带,其余均为着丝粒C-带;计算其异染色质含量为30％;Ag-NORs带型特征为具有1对Ag-NORs,位于2号染色体的长臂末端,为端部Ag-NORs; G-带特征为具有38条深染带,21条浅染带.研究结果为圆斑星鲽染色体组学和种质资源保护提供了基础资料.  相似文献   

东海银鲳卵巢周年变化的组织学观察   总被引：16，自引：5，他引：16  

龚启祥  倪海儿  李伦平  郑春静 《水产学报》1989,13(4):316-325

本文对东海银鲳的卵巢周年变化、卵母细胞发育等作了研究。结果表明,东海银鲳的繁殖期在5—6月。繁殖期过后,其卵巢发育长期处在重复发育的Ⅱ′期,并在此期过冬,至翌年3月下旬卵巢再次发育。在卵母细胞发育早期,胞质内便出现油球结构,胞质有分层现象。卵母细胞发育至大生长期晚期后,其放射带减薄且渐消失。待卵母细胞充分发育时,仅存单层的胶质膜结构。银鲳为短期分批产卵类型。  相似文献