苍梧县公益林经营管理现状与可持续经营探讨   总被引：2，自引：0，他引：2  

张伟  金大刚  周华生 《中南林业调查规划》2006,25(1):13-15,19

调查分析广西苍梧县生态公益林建设的现状和存在的问题，主要有树种结构欠合理、权益关系不清、经营者的意愿未得到尊重、相关法规不完善等，对该县生态公益林可持续发展对策进行探讨。  相似文献   

5个草地型草地牧草的生长速率和养分动态研究     

袁福锦吴文荣  金显栋奎嘉祥 《四川草原》2005,(3):24-27

在牧草生长季节(5~9月)对小哨牧场放牧演替形成的5个草地型草地的牧草进行生长速率、牧草产量及养分的逐月观测,生长速率测定显示,白三叶+东非狼尾草和东非狼尾草+非洲狗尾草两个草地型草地均出现两个生长峰值,分别在6月和9月,其余3个草地型草地只有一个生长高峰。干物质产量和牧草养分测定结果表明,白三叶+东非狼尾草和白三叶+非洲狗尾草+画眉草两种草地型草地表现最好,可以在气候环境条件类似的地区推广种植利用;白三叶+画眉草和白三叶+非洲狗尾草两种草地型草地表现优良,可以在草地改良中,根据当地实际情况选择性地加以种植;东非狼尾草+非洲狗尾草型草地表现较差,不提倡种植。  相似文献   

鸡传染性腔上囊病病毒VP2基因在昆虫细胞中的表达   总被引：4，自引：0，他引：4  

李迎晓秦爱建  刘红梅邵红霞金文杰  刘岳龙 《中国兽医科技》2005,35(12):933-936

为了深入研究鸡传染性腔上囊病病毒（IBDV）VP2基因的结构和功能,利用Bac-to-Bac系统研制出含有VP2基因的重组杆状病毒rBac-VP2,将rBac-VP2感染Sf9细胞,并用抗IBDV VP2特异性单克隆抗体经间接免疫荧光试验检测,证实感染重组病毒Sf9细胞能高效表达IBDV VP2基因产物;Western-blotting分析结果表明,VP2基因表达产物的分子质量约为40 ku.  相似文献   

番鸭在南京地区的适应性观察     

伍清林  金兰梅  王明海  范亦刚  殷久祥 《家畜生态》2005,(5)

番鸭在南京地区进行冬、夏两批饲养,均有较强的适应性。试验结果表明:冬、夏两 批饲养的番鸭只平均重量差异不显著(P>0．05)。夏季与冬季的料重比分别为1:2．62和1: 3．07,冬季饲养的鸭子饲料利用率低,料重比大于夏季。冬季在塑料大棚中饲养番鸭精饲料减 少1／3,添加青粗饲料能达到同样的增重效果。温度与增重无相关关系,相对湿度与番鸭的增 重呈负相关(P<0．01)。鸭舍内平均温度在11．05℃-30．93℃之间,相对湿度在76．28％以 下,番鸭能正常生长发育。环境温度在9．8℃以下,番鸭的生长发育受阻,饲料利用率降低。相 对湿度在77％以上,其增重受到影响。特别在低温高湿的环境中,严重地影响番鸭的生长。在 相同的饲养期内,周平均温度9．8℃,平均相对湿度91％,番鸭周平均增重22 g,周平均温度 27．42℃,平均相对湿度71．29％,番鸭周平均增重355 g,相差333 g(P<0．01)。  相似文献   

Effect of moxonidine hydrochloride on left ventricular hypertrophy in spontaneously hypertensive rats     

ZHANG Peng-bo  NIU Xiao-lin  SUN Yu-ming  CHANG Dan  JINYao-feng 《园艺学报》2003,19(8):1089-1091

AIM:To investate the effect of domestric moxonidine hydrochloride on myocardium fibrosis and coronary artery microvascular structure in left ventricular hypertrophy of spontaneously hypertensive rats(SHR).METHODS:30 male SHR, aged 20 weeks, were divided into group Mox+SHR, Cap+SHR and SHR randomly (10 in each group). 10 age and sex-mached sprauge-dawley rats were designed as normal control(NC). At the end of 13 weeks, left ventricular wight/body weight ratio(LVW/BW), collagen volume fraction(CVF) and standardized perivascular collagen area(PVCA) as well as intramyocardial arterial average medial thickness (AMT) were determined.RESULTS:LVW/BW, CVF, PVCA and AMT in group Mox+SHR were lower significantly than that in group SHR, respectively.CONCLUSION:Long-term antihypertensive treatment with moxonidine hydrochloride reduces myocardium fibrosis and improves impaired coronary artery microvascular structure in left ventricular hypertrophy.  相似文献   

Cloning of p15^INK4b related gene CCRG and expression analysis in the renal carcinoma     

JIN Gui-hua  YE Xiong-jun  CHANG Zhi-jie 《园艺学报》2003,19(10):1300-1304

AIM and METHODS:To analysis the factor that involved in renal carcinogenesis, we used the bait gene AK001518 to screen GenBank. To understand the relationship between cell cycle related gene(CCRG) and p15, we did RT-PCR and Northern Blot experiments. Then we examined CCRG expression level in renal carcinogenesis. RESULTS:Gained a function unknown gene CCRG that was 67% a mino acid identical with the gene AK001518 that was regulated by p15. It was shown that the CCRG mRNA was dramatically decreased when p15 gene was over-expressed. CCRG expression level was much higher in tumor tissues and cells than normal tissues and cells. CONCLUSION:The novel gene CCRG expressed highly in the renal carcinoma, which might play a significant role in the renal carcinogenesis.  相似文献   

玉米疯顶病种子传播研究   总被引：2，自引：0，他引：2  

杨建国  金晓华  谢爱婷  王晓鸣  朱振东 《中国植保导刊》2002,22(6):3-4

连续3a对不同来源的玉米种子,利用显微镜镜检和试种观察植株发病显症的方法,检测其携带玉米疯顶病病菌状况。结果表明,病株病穗籽粒、病株健穗籽粒、病田健株籽粒都有较高的带菌率。因此,病菌随种子调运远距离传播是造成各地病害突发的主要原因。  相似文献   

地高辛标记核酸探针的标记方法及其在食用菌研究领域的应用   总被引：2，自引：0，他引：2  

葛丹  陈明杰  曹文伟 《食用菌学报》2002,9(2):50-55

综述了非放射性地高辛（DIG）标记系统的原理和主要特点，介绍了地高辛标记核酸探针的主要标记方法，影响探针标记方法选择的因素，标记探针的显色检测方法及其在食用菌研究领域的应用。  相似文献   

Influences of chronic hypoxia on the gene expression of Kv1.3,Kv2.1 and Kv3.1 induced by acute hypoxia in PASMC of rats     

HONG Zhi-gang  JIN Si  KONG Wei  WANG Di-xun  CHEN Qi-ling  SUN Bing-yong 《园艺学报》2002,18(10):1192-1195

AIM and METHODS: Total RNA was extracted from 6th rat subcultured pulmonary artery smooth muscle cells(PASMC) exposed to continual chronic hypoxia or normoxia and the effects of chronic hypoxia on the changes of Kv1.3,Kv2.1,Kv3.1 mRNA in cultured PASMC induced by acute hypoxia were studied by semiquantitative RT-PCR in vitro. RESULTS:①Kv1.3,Kv2.1,Kv3.1 genes were found to be expressed in PASMC of rats exposed either to hypoxia or normxia.②The expression of Kv2.1 and Kv3.1 in 6th subcultured of PASMC in normaxia group could be upregulated by exposure to acute hypoxia,the levels of Kv2.1 and Kv3.1 mRNA were significantly increased from 0.646±0.092, 0.782±0.104 to 1.059±0.134, 0.985±0.116,respectively (P<0.01,n=5). ③PASMC cultured continuously in chronic hypoxia for 6 subcultures and then exposed to normoxia for 12 h,thereafter the expression of Kv2.1 and Kv3.1 were downregulated by acute hypoxia for 6 hours.The level of Kv2.1 mRNA was significantly decreased from 1.008±0.117 to 0.649±0.097 (P<0.01,n=5). CONCLUSION:Kv2.1,Kv3.1 genes might be oxygen sensitive genes.Chronic hypoxia might change the response of these Kv genes of PASMC to acute hypoxia and down-regulate its expression,which might probably decrease the role of Kv in HPV.  相似文献   

蛹虫草人工优质高产栽培技术研究   总被引：17，自引：1，他引：17  

陈艳秋  朴日子  金英善  严花淑 《中国食用菌》2002,21(5):20-22

本试验通过对四个人工分离蛹虫草品种的菌丝体生长情况、子实体产量进行对比分析，结果显示：沈阳人工分离种（S）为优质品种，武汉（W）、河南（H）、北京（B）种为淘汰种。以大米加4个蚕蛹为基质的培养基子实体生物学效率最高；液体菌种生长周期短，生物学效率较高。  相似文献