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61.

Context

Freshwater ecosystems depend on surrounding terrestrial landscape for resources. Most important are terrestrial leaf litter subsidies, which differ depending on land use. We lack a good understanding of the variation of these inputs across spatial scales.

Objectives

We sought to determine: (1) the relative importance of local versus catchment-level forestation for benthic leaf litter biomass in streams, (2) how landscape configuration alters these relationships, and (3) how land use affects the quality and diversity of leaf litter subsidies.

Methods

We measured biomass and identity of benthic leaf litter in 121 reaches in 10 independent catchments seasonally over the course of a year. We assessed direct and indirect effects of forestation, reach position, and seasonality on leaf litter biomass using structural equation models, and assessed how leaf litter diversity varied with land use.

Results

In catchments with forested headwaters, the degree of forestation and reach position in the catchment influenced benthic leaf litter biomass indirectly through local reach-scale forestation. In catchments where forest was only located downstream, or with minimal forest, none of these factors influenced reach-level benthic leaf litter. Leaf litter diversity peaked in fall in all land use types, but was generally lowest in forested reaches.

Conclusions

Not only habitat amount, but its location relative to other habitats is important for ecosystem function in the context of cross-ecosystem material flows. Here, lack of upstream forest altered spatial patterns of leaf litter storage. Studies with high spatiotemporal resolution may further reveal effects of landscape configuration on other ecosystems.
  相似文献   
62.
Reconciling food security, economic development and biodiversity conservation is a key challenge, especially in the face of the demographic transition characterizing many countries in the world. Fisheries and marine ecosystems constitute a difficult application of this bio‐economic challenge. Many experts and scientists advocate an ecosystem approach to manage marine socio‐ecosystems for their sustainability and resilience. However, the ways by which to operationalize ecosystem‐based fisheries management (EBFM) remain poorly specified. We propose a specific methodological framework—viability modelling—to do so. We show how viability modelling can be applied using four contrasted case‐studies: two small‐scale fisheries in South America and Pacific and two larger‐scale fisheries in Europe and Australia. The four fisheries are analysed using the same modelling framework, structured around a set of common methods, indicators and scenarios. The calibrated models are dynamic, multispecies and multifleet and account for various sources of uncertainty. A multicriteria evaluation is used to assess the scenarios’ outcomes over a long time horizon with different constraints based on ecological, social and economic reference points. Results show to what extent the bio‐economic and ecosystem risks associated with the adoption of status quo strategies are relatively high and challenge the implementation of EBFM. In contrast, strategies called ecoviability or co‐viability strategies, that aim at satisfying the viability constraints, reduce significantly these ecological and economic risks and promote EBFM. The gains associated with those ecoviability strategies, however, decrease with the intensity of regulations imposed on these fisheries.  相似文献   
63.
Background: The range of American canine hepatozoonosis (ACH) is expanding from the southern USA northward. Transmission of Hepatozoon americanum occurs by ingestion of infected Gulf Coast ticks, Amblyomma maculatum. The source of the protozoan for the tick remains undetermined; infected dogs are unusual hosts for the tick. Objective: Compare possible sources of infection by field investigations of 2 multiple‐dog outbreaks of ACH. Animals: Twenty‐eight privately owned dogs (Canis familiaris), 1 coyote (Canis latrans), 31 wild‐trapped cotton rats (Sigmodon hispidus), 24 wild‐trapped field mice (Peromyscus leucopus), and 9 wild‐caught rabbits (Sylvilagus spp.) from sites in eastern Oklahoma were monitored for hepatozoonosis. Six laboratory‐raised cotton rats (S. hispidus), 6 Sprague‐Dawley rats (Rattus norvegicus), 6 C57BL/6J‐Lystbg‐J/J mice (Mus musculus), 6 outbred white mice (M. musculus), 6 New Zealand white rabbits (Oryctolagus cuniculus), and 2 dogs were acquired through commercial vendors for experimental transmission trials of H. americanum. Methods: Four of 15 dogs in a rural neighborhood and 5/12 hunting Beagles were confirmed to be infected by blood smear examination, muscle biopsy, and polymerase chain reaction assay of the 18S rRNA gene of Hepatozoon species. Histories and tick host preferences led to field collections of common prey of canids and experimental transmission trials of H. americanum to selected prey (M. musculus, S. hispidus, R. norvegicus, and O. cuniculus). Results: Dogs with ready access to prey (4/15 dogs) or that were fed prey retrieved from hunts (5/12 hunting Beagles) became infected, providing evidence that predation is an important epidemiologic component of ACH infection. Experimental transmission studies identified a quiescent, infectious stage (cystozoite) of the parasite that provides an alternate mode of transmission to canids through predation, demonstrating that cotton rats, mice, and rabbits but not brown rats may act as paratenic hosts of H. americanum. Conclusions and Clinical Importance: Predation of prey harboring infected A. maculatum or containing cystozoites of H. americanum in their tissues provide 2 modes of transmission of ACH to dogs, putting unconfined dogs at increased risk of infection in endemic areas.  相似文献   
64.
The effect of high temperatures (above 25°C) on starch concentration and the morphology of starch granules in the grains of wheat (Triticum aestivum L.) were studied. Wheat plants of cultivars Yangmai 9 (weak-gluten) and Yangmai 12 (medium-gluten) were treated with high temperatures for 3 days at different times after anthesis. The results showed that the starch concentration of grains given a heat-shock treatment above 30°C were lower than those developing at normal temperature in both cultivars. High temperature lowered starch concentration due to the decrease of amylopectin. Under the same temperature, the effect of heat shock from 6 to 8 days after anthesis (DAA) was the greatest, whereas from 36 to 38 DAA the effect was the least. The effects of high temperatures after anthesis on starch-pasting properties were similar to those on starch concentration, especially after 35-40°C treatments. The size, shape and structure of starch granules in wheat grains (determined by electron microscopy) after heat shock were visibly different from the control. When given heat shock during development, the starch granules in mature wheat grains were ellipsoid in shape and bound loosely with a protein sheath in Yangmai 9, while they were damaged and compressed with fissures in Yangmai 12, indicating the differences in resistance to high temperature between cultivars. Ratios of large (type-A) and small (type-B) starch granules significantly decreased under heat shock, which limited the potential sink size for dry matter deposition in the grain.  相似文献   
65.
66.
The species composition of a plant community can affect the distribution and abundance of other organisms including plant pathogens. The goal of this study was to understand the role of host diversity in the transmission of two Barley yellow dwarf virus (BYDV) species that share insect vectors and hosts. Greenhouse experiments measured the transmission rate of BYDV species PAV and PAS from infected oat plants to healthy agricultural and wild grasses and from these species back to healthy oat seedlings. In the field component of the study, the rate of spread of PAV and PAS was measured in monoculture plots planted with agricultural grasses. In greenhouse experiments, the aphid vector more readily transmitted PAV from agricultural grasses and more readily inoculated PAS to the wild grass species assayed. In the field experiment, disease prevalence was greater in wheat, but there was no difference in the rate of spread of PAV and PAS. These results indicate an interaction between vector and host genotype that selects for greater PAV transmission in grain crops, contributes to differences in disease prevalence between grass types, and maintains pathogen diversity within the larger plant community (i.e. agricultural and non‐agricultural hosts).  相似文献   
67.
Vermicomposts are produced through interactions between earthworms and microorganisms in the breakdown of organic wastes. Aqueous extracts were prepared in commercial brewing equipment (Growing Solutions Inc.) from vermicomposts processed from pre-consumer food waste. The ratio of vermicompost to water was 1 to 5 v:v to produce a 20% aqueous solution. The effects of soil drenches at dilutions of 20%, 10%, and 5% vermicompost extracts, were compared with those of deionized water, in the suppression of green peach aphids, mealybugs, and two spotted spider mites attacking tomatoes and cucumbers, in greenhouse cage experiments.  相似文献   
68.
Fusarium spp. is among the largest and most important pathogen groups that attack major grain crops including sorghum. The objectives of this study were to compare the virulence of Fusarium spp. associated with sorghum and examine the mode of resistance of genotypes to the disease. Eight Fusarium species – F. verticillioides, F. thapsinum, F. andiyazi, F. proliferatum, F. nyagamai, F. pseudoanthophilum, F. brevicatenulatum, and F. pseudonygamai – were studied using three sorghum genotypes under greenhouse condition. Three of these pathogens (F. verticillioides, F. thapsinum, and F. andiyazi) were selected for genetic analysis of resistance under field conditions. Sorghum genotypes with contrasting stalk rot reactions were selected for use in both the greenhouse and field experiments. Two weeks after flowering, plants were inoculated with liquid inoculum culture (5 × 104 conidia ml−1) of the different pathogens. Plants were harvested 4 weeks after inoculation and rated for disease severity on the basis of lesion length and number of nodes crossed by the lesion. Among the pathogens, F. thapsinum resulted in consistently higher disease scores in all genotypes under all environments. Likewise, genotype SC599 showed the greatest and most stable resistance as inbred as well as in hybrid combinations as shown by consistently high GCA for resistance to all pathogen species. We recommend that future screening exercises for Fusarium stalk rot resistance utilize F. thapsinum as the causal organism and include the resistant genotype SC599 as a control.  相似文献   
69.
70.
Objective – To compare the placement feasibility and amount of bone trauma induced by 3 intraosseous (IO) access techniques in cats: an automatic impact penetration device (A), an automatic rotary insertion device (B), and a manual IO needle (C). Design – Prospective ex vivo study. Setting – University. Animals – Eighteen adult mixed breed feline cadavers. Interventions – Cadavers provided 72 total IO insertion locations divided equally between the right and left humerus and tibia. The 3 IO techniques were randomly allocated to these locations. Time to successful insertion, ease of insertion, and success rate were recorded. Each insertion site was analyzed for the number of bone fragments and defect diameter by computed tomography. Measurements and Main Results – Device B had lower time of insertion (P=0.01) compared with devices A and C. Device B had better ease of insertion scores (P<0.01) compared with devices A and C. No differences were detected between insertion sites (tibia versus humerus). No differences in the number of bone fragments, defect diameter, or success rate were detected among devices (P=0.06, 0.31, and 0.14, respectively). Conclusions – All 3 IO access methods evaluated yield acceptable results. Device B is significantly faster and easier to place in cat cadavers when compared with other methods.  相似文献   
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