香椿组织培养与快速繁殖的研究   总被引：6，自引：2，他引：6  

康明  张树婷 《经济林研究》1993,11(2):69-72,78

香椿试管苗在不同培养条件下增殖速度明显不同.太行山香椿试管苗的最佳增殖条件为改良MS十SBA1.0十GA_32.0+IAA0.0;燕山香椿试管苗则为WPM+6BA1.0+GA_32.0+IAA0.0。试管苗的生根能力在IBA0.2～1.0的范围内逐渐提高.增殖倍数不同试管苗其K的摄入量、碳水化合物含量以及POD和EST同工酶图谱亦有明显差别。  相似文献   

Genetic Relationships among Prunus mume var. pendula Using AFLP Markers     

MING Jun  Zhang Qixiang  Ru Guangxin  Mao Qingshan  Yan Xiaolan  Lan Yanping 《中国林学(英文版)》2003,5(4)

Genetic relationships among Prunus mume var. pendula were studied by using AFLP markers. 18 accessions representing 14 cultivars ofPrunus murne var. pendula were selected from the germplasm collection at the Research Center of China Mci Flower. Seven Mse I-EcoR I AFLP primer combinations revealed 450 legible bands, and 269 of which were polymorphic markers. A similarity matrix was prepared using the simple matching coefficient of similarity and Nei‘s (72) distance coefficient. A UPGMA dendrogram demonstrated the genetic relationships of the cultivars. The information given by AFLP markers was basically consistent with the morphological classification and the evolutionary history of the morphotypes, and roughly supported the new revised classification system for Chinese Mci Cultivars. But there were still several exceptions: 1) the ‘Guhong Chuizhi‘ inserted between the ‘Tiaoxue Chuizhi‘ and the ‘Danfen Chuizhi‘; 2) the ‘Wufu Chuizhi‘ kept off the Pink Pendant Form, and the ‘Moshan Chuizhi‘ was removed from Viridiflora Pendant Form; 3) the ‘Danbi Chuizhi‘ and the ‘Shuangbi Chuizhi‘ of Viridiflora Pendant Form got together well but fell within the Pink Pendant Form.  相似文献   

森林经营类型的划分及应用   总被引：2，自引：0，他引：2  

明海军  蒋慧  刘春革 《内蒙古林业调查设计》2006,29(1):61-62,65

森林分类经营实施后,森林经营类型已成为森林经营过程中合理经营和科学管理的重要依据,文章就林区森林经营类型划分及其在森林经营方案、森林监测中的应用进行了论述。  相似文献   

河南桧柏嫩枝“全光雾”扦插育苗试验初报     

丁琦  明照军 《内蒙古林业科技》2002,(2):8-10

河南桧柏采用嫩枝“全光雾”扦插育苗，试验表明：对难生根珍贵树种用此法育苗可大大缩短育苗周期，提高苗木产量和质量，应大力推广此种育苗方法。  相似文献   

空肠弯曲菌致GBS动物模型的建立     

薛峰  曾德新  徐飞  杨跃飞  王小波  张小荣  李震中  蒋原 《中国动物检疫》2015,32(2):19-23

[目的]利用巴马小香猪构建空肠弯曲菌致GBS的动物模型。[方法]选取致人GBS源空肠弯曲菌株,采用3×1012 cfu/m L、3×1011 cfu/m L、3×1010 cfu/m L、3×109 cfu/m L等浓度对4组巴马小型猪灌胃攻毒,每头猪攻毒10m L,同时设立对照组。从第二周开始定期采集组织样及血液样本,通过免疫学检测及组织病理切片判定病变情况。[结果]免疫学检测发现,在16份攻毒组样品中有12份血清中GM1-Ig G抗体检测阳性,阳性检出率为81.25%。但是,并没有显著的攻毒浓度梯度差异。病理组织切片H/E染色显示,抗体阳性试验猪的大脑出现了噬神经元、小血管周围间隙增宽、神经中央染色溶解等现象;切片LFB染色显示,在发病模型动物的小脑白质和腰膨大出现了轻度脱髓鞘现象。[结论]结果表明空肠弯曲菌诱导GBS的浓度在3×109 cfu/m L~3×1012 cfu/m L均可,组织样本及血液采集在攻毒后4~5周较理想。  相似文献   

气候与种植结构变化对温室气体排放及灌溉需水的影响     

栾晓波  高子涵  薛静  孙世坤  唐一荷  高飞 《农业机械学报》2023,54(12):327-337

针对气候变化和种植结构调整对黄淮海地区农业生产作用不明晰的问题,基于反硝化分解模型(Denitrification-decomposition, DNDC),采用情景分析法评估了气候与种植结构变化对黄淮海地区农业温室气体(CO₂、CH₄和N₂O)排放和灌溉需水量的影响。结果表明：从1995年到2015年,研究区气候向暖湿化方向发展,其中年均最高温度无显著变化,年均最低温度上升0.7℃,年降水量增长46.5 mm; 1995年研究区玉米、小麦和水稻种植面积分别约为7.9×10⁶、1.4×10⁷、2.9×10⁶ hm²;2015年3种作物种植面积均增大,而水稻、小麦种植比例减小。气候变化影响下,黄淮海地区农业温室气体排放增加,灌溉需水量小幅减小。与1995年相比,2015年CO₂、CH₄、N₂O排放强度分别增长至3 730.5、443.2、5.9 kg/hm²  相似文献   

远程监测技术在港口气体污染物监测中的应用研究     

陈秋燕  李晓波  李珂  赵淑媛 《绿色科技》2021,(6):72-74

对青岛前湾港进行实地考察,确定了港口监测设备布点位置,通过试验验证,论证了监测设备距船舶的距离、风向与船舶航行的测试夹角、湿度、环境温度等参数对港口船舶运行过程中烟气扩散测试结果的影响,同时,确定了影响船舶气态污染物监测的关键因素,为在我国港口搭建船舶排放监测系统提供一些参考。  相似文献   

Dynamics of maize grain drying in the high latitude region of Northeast China          下载免费PDF全文

CHU Zhen-dong  MING Bo LI Lu-lu  XUE Jun  ZHANG Wan-xu  HOU Liang-yu  XIE Rui-zhi  HOU Peng  WANG Ke-ru  LI Shao-kun 《农业科学学报》2022,21(2):365-374

A high grain moisture content at harvest has been an important problem in the high latitude region of Northeast China, and it is closely related to the genotypes of varieties, local meteorological factors and planting management. However, delayed harvest at a low temperature could not effectively reduce the grain moisture content. In this study, we continuously observed the grain drying during the late stage of different maturing types of maize varieties in Daqing, Heilongjiang Province, China in 2016 and 2017. A two-segment linear model was used to analyze the different stages of the drying processes: 1) Two-segment linear model fitting can divide the grain drying process of all varieties into two separate linear drying processes with different slopes. 2) During the rapid drying stage, the drying was faster at a higher temperature. The rate of slow drying was influenced by air vapor pressure. 3) The moisture content and meteorological factors when the drying rate turns from one stage into the other were not consistent between varieties and years. After entering the frost period, temperatures below 0°C will significantly reduce the rate of grain drying. 4) Due to the short growth period of early-maturing varieties, the drying time was prolonged, and the grain moisture content was lower than that of the mid-late maturing varieties. Local meteorological conditions do not allow the drying of mid-late maturing varieties to achieve a lower moisture content. When the temperature falls below 0°C, the drying rate of grain decreases markedly. Therefore, one feasible way to solve the problem of high moisture content is to replace the early-maturing varieties and implement the corresponding cultivation techniques.  相似文献   

茉莉酸对马铃薯苗期低温胁迫耐性的影响     

刘计涛  王梦诗  索海翠  罗焕明  王丽  李成晨  单建伟  廖永珊  李小波 《广东农业科学》2021,48(12):129-137

[目的]研究不同茉莉酸(JA)施用浓度对马铃薯叶片活性氧(ROS)含量及其清除酶活性以及抗寒关键基因CBF表达的影响,初步确定提升马铃薯抗寒性的最适施用浓度,为马铃薯产业的发展提供一定的理论基础.[方法]以马铃薯品种费乌瑞它为研究对象,采用叶面喷施JA的方法,探究正常和低温胁迫条件下,植株叶片的活性氧含量,活性氧清除酶过氧化氢酶(CAT)、过氧化物酶(POD)和超氧化物歧化酶SOD活性,以及抗寒关键基因CBF的表达.[结果]随着JA浓度的增加,马铃薯苗期叶片的活性氧含量逐渐降低,低温胁迫后活性氧含量能维持在较低水平,适宜浓度范围为100～400μmol/L,以200μmol/L JA处理效果最好,ROS含量降低47.6％.JA能够提升活性氧清除酶CAT和POD活性,且随着JA浓度增加效果越显著,低温胁迫后200μmol/L JA处理分别显著提升1.30、2.53倍.正常条件下,JA处理抑制SOD活性最低达70.7％;低温胁迫条件下,JA又可能通过其他途径提升SOD活性,最高达2.11倍.外源JA能诱导CBF1-3基因的表达,而低温胁迫能促进内源JA的合成,诱导CBF1-3基因的表达,提升马铃薯的抗寒性.[结论]马铃薯苗期的抗寒性与外源施加JA的浓度具有明显的相关性,适宜的JA浓度能够促进抗寒关键基因CBF1-3的表达,显著提升CAT、POD、SOD的活性,抑制低温胁胁迫诱导的活性氧生成,提高马铃薯抗寒性.适宜施用JA浓度为100～400μmol/L,以200μmol/L效果最佳.  相似文献   

高通量测序分析蚕豆种子内生细菌的多样性     

刘璐  名晓东  张晓艳  郝俊杰  付丽平  王乾坤  吕鑫  陈旺  刘全兰 《中国农业科技导报》2021,23(2):73-80

为了分析蚕豆种子内生细菌的多样性,采用Illumina MiSeq高通量测序技术,从日本大白皮（S18P23.1、 S18P23.2）和启豆2号（S18P24.1、S18P24.2）的种子获得16S RNA V3～V4区有效序列133 855条。将高于97％相似度的序列划分为一个操作分类单元（operational taxonomic unit, OTU）,优化后得到1 598个OTUs。内生细菌种群分析结果表明,拟杆菌门（Bacteroidetes,丰度为30%～33%）、变形菌门（Proteobacteria, 23%～25%）、厚壁菌门（Firmicutes, 23%～25%）和放线菌门（Actinobacteria, 5%～7%）为两个蚕豆品种共有的优势菌门,但属水平的优势菌群在供试蚕豆种子中均有差异。这些结果表明,蚕豆种子具有丰富的内生细菌资源,含有多种具有益功能性状的细菌类群,值得进一步跟踪研究这些益生菌在蚕豆种植到土壤后的变化规律;启豆2号种子中益生菌的种类和丰度高于日本大白皮,值得进一步分离和研究这些益生菌的益生或促生特性,筛选获得在食品和生物肥料等领域有应用潜力的菌株。  相似文献