Using graph theory to analyse and assess changes in Mediterranean woodland connectivity     

Machado  Rui  Godinho  Sérgio  Guiomar  Nuno  Gil  Artur  Pirnat  Janez 《Landscape Ecology》2020,35(6):1291-1308

Landscape Ecology - The Portuguese montado is an agro-silvopastoral system, similar to the Spanish dehesa, known for its cultural, economic and ecological value. Despite its importance, contrasting...  相似文献   

Tolerance to nymphs and adults of Mahanarva spectabilis (Hemiptera: Cercopidae) by forage plants in fertilized soils     

Roberta Alvarenga  Alexander Machado Auad  Jair Campos Moraes  Sandra Elisa Barbosa da Silva  Brunno Santos Rodrigues 《Pest management science》2019,75(8):2242-2250

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Associations between morphometric variables and weight and yields carcass in Pirapitinga Piaractus brachypomus     

Fagner Machado Ribeiro  Mrio Lima  Pedro Aurlio Tataíra da Costa  Diego Machado Pereira  Thony Assis Carvalho  Tadeu Vilela de Souza  Hortência Aparecida Botelho  Fabyano Fonseca e Silva  Adriano Carvalho Costa 《Aquaculture Research》2019,50(7):2004-2011

The objective of this work was to evaluate the association between morphometric variables and carcass characteristics in Pirapitinga. We used a thousand specimens of Pirapitinga with an average weight of 1,200 g, which were stunned, slaughtered, weighed, measured, and processed for morphometric and processing yield analysis, to obtain weights, carcass and fillet yields. Initially, the linearity of the variables was verified. Pearson's simple and partial correlation tests were performed between all metrics. Track analysis was performed considering the weights and yields of carcass and filet as dependent variables and the others as independent variables. Ridge regression models were used to eliminate the effects of multicollinearity among the independent variables. Observations showed that the simple correlations between body weights and yields were superior to the partial ones in terms of magnitude. The length and circumference of the specimens were the measures most linearly associated with weight, carcass weight and fillet with rib weight. As for carcass yield and fillet yield, linear correlation coefficients were low and not significant when associated with body weights. With the path analysis, we could observe significant positive correlations between the morphometric measurements for weight, carcass weight and fillet with rib weight. The standard length, body circumference and body circumference/body height ratio are the most correlated. The latter are the most important variables in the evaluation of body weights in Pirapitinga fish. They can serve as criteria for indirect selection in searching for fish with better carcass characteristics. As for fillet and carcass yields, the morphometric variables have not shown to be efficient for indirect selection.  相似文献   

Morphological and morphometric characterization of domestic cat epididymal sperm     

Brenna de Sousa Barbosa  Herlon Victor Rodrigues Silva  Beatriz Evaristo de Almeida Tabosa  Thalles Gothardo Pereira Nunes  Francisco Felipe de Magalhes  Lúcia Daniel Machado da Silva 《Reproduction in domestic animals》2019,54(12):1630-1636

Sperm morphometry is the tool that confers objectivity to the morphological evaluation by accurately measuring the dimensions of the gamete and its structures. Thus, the aim of the study was to perform a morphometric characterization of the domestic cat sperm. Therefore, sperm samples were collected from twenty pairs of epididymis in a TRIS extender at 37ºC. An aliquot of the sample was used to make a smear with Rose Bengal solution, and afterwards, the morphology and morphometry were analysed. In the morphology, were quantified the percentage of normal sperm cells, morphological changes of head, midpiece and tail. In morphometry, each normal sperm cell was measured for length, width, area and perimeter of head and midpiece, tail length and total length. The parameters ellipticity, elongation, regularity and rugosity were also determined. The percentage of normal sperm was 67.21%. Of the abnormalities, the curled/folded tail, followed by the curved midpiece, abnormal shaped head and detached head were the most quantified. The sperm head presented 5.56 ± 0.01 μm and 3.10 ± 0.01 μm of length and width, respectively. The head area was 16.94 ± 0.05 μm², while the perimeter was 16.16 ± 0.03 μm. In the derived parameters, the values were as follows: ellipticity of 1.81 ± 0.00; elongation of 21.39 ± 0.12; regularity of 0.81 ± 0.00; and rugosity of 0.14 ± 0.00. The midpiece presented length and width of 7.96 ± 0.01 μm and 0.76 ± 0.01 μm, respectively. The mean length of the sperm tail was 45.12 ± 0.06 μm, and the total cell size was 58.67 ± 0.06 μm. Thus, it was concluded that the cat sperm is an elongated cell, with high rugosity and regularity. The spermatic tail represents more than ¾ of the total length of the cell and the midpiece exceeds the length of the head.  相似文献   

Intake,digestibility, microbial protein production,and nitrogen balance of lambs fed with sorghum silage partially replaced with dehydrated fruit by-products     

Almeida  Júlio César Silva  de Figueiredo  Darcilene Maria  de Azevedo  Katharine Kelly  Paixão  Mônica Lopes  Ribeiro  Enilson Geraldo  Dallago  Gabriel Machado 《Tropical animal health and production》2019,51(3):619-627

Tropical Animal Health and Production - The objective of this study was to evaluate intake, digestibility, microbial protein production, and nitrogen balance of lambs fed with different dehydrated...  相似文献   

Canine ovariectomy by hybrid or total natural orifice transluminal endoscopic surgery: technical feasibility study and pain assessment     

Marcella Teixeira Linhares  Joo Pedro Scussel Feranti  Gabriela Pesamosca Coradini  Letícia Reginato Martins  Arthur Rodrigues Martins  Vanessa Zanchi Sarturi  Felipe Baldissarella Gavioli  Marco Augusto Machado Silva  Michelli Westphal de Ataíde  Luciana Gonalves Teixeira  Maurício Veloso Brun 《Veterinary surgery : VS》2019,48(Z1):O74-O82

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Interactive effects of dietary fishmeal level and plant essential oils supplementation on European sea bass,Dicentrarchus labrax: Growth performance,nutrient utilization,and immunological response     

Rui A. Gonalves  Renata Serradeiro  Marina Machado  Benjamin Costas  Christine Hunger  Jorge Dias 《Journal of the World Aquaculture Society》2019,50(6):1078-1092

The inclusion of phytogenics in fish feed is a promising strategy to compensate for the negative performance effects of replacing fishmeal (FM) with vegetable sources. The present work assessed the interactive effects of different dietary FM levels (22.5 and 10% of formulation) and the supplementation of a commercial blend of anise, citrus, and oregano essential oils (Digestarom PEP M.G.E 150) on European sea bass, Dicentrarchus labrax growth performance, nutrient utilization, gut morphology, antioxidant status, and immunological response over a 60‐day growth trial. Results showed decreased growth and protein efficiency ratio and increased feed conversion ratio with a low dietary FM level. In contrast, supplementation of the phytogenic product demonstrated improved performance and nutrient utilization together with increased protein and energy retention. Supplementation with the plant essential oils fully compensated for the negative intestinal changes observed in sea bass fed a low‐FM diet but showed little improvement in fish immunological response, except for the 30% increase in lysozyme activity observed in fish fed the low FM‐supplemented diet compared to those fed the standard high‐FM diet. Overall, this study supports the use of this phytogenic product in low‐FM diets as a possible tool to decrease feed costs associated with FM without compromising fish performance, nutrient utilization, and health.  相似文献   

Practical implications of using non-relational databases to store large genomic data files and novel phenotypes     

Andr Moreira Souza  Rodrigo de Andrade Santos Weigert  Elaine Parros Machado de Sousa  Lucas Tassoni Andrietta  Ricardo Vieira Ventura 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2022,139(1):100-112

The objective of our study was to provide practical directions on the storage of genomic information and novel phenotypes (treated here as unstructured data) using a non-relational database. The MongoDB technology was assessed for this purpose, enabling frequent data transactions involving numerous individuals under genetic evaluation. Our study investigated different genomic (Illumina Final Report, PLINK, 0125, FASTQ, and VCF formats) and phenotypic (including media files) information, using both real and simulated datasets. Advantages of our centralized database concept include the sublinear running time for queries after increasing the number of samples/markers exponentially, in addition to the comprehensive management of distinct data formats while searching for specific genomic regions. A comparison of our non-relational and generic solution, with an existing relational approach (developed for tabular data types using 2 bits to store genotypes), showed reduced importing time to handle 50M SNPs (PLINK format) achieved by the relational schema. Our experimental results also reinforce that data conversion is a costly step required to manage genomic data into both relational and non-relational database systems, and therefore, must be carefully treated for large applications.  相似文献   

Dark respiration rate increases with plant size in saplings of three temperate tree species despite decreasing tissue nitrogen and nonstructural carbohydrates     

Machado JL  Reich PB 《Tree physiology》2006,26(7):915-923

In shaded environments, minimizing dark respiration during growth could be an important aspect of maintaining a positive whole-plant net carbon balance. Changes with plant size in both biomass distribution to different tissue types and mass-specific respiration rates (R(d)) of those tissues would have an impact on whole-plant respiration. In this paper, we evaluated size-related variation in R(d), biomass distribution, and nitrogen (N) and total nonstructural carbohydrate (TNC) concentrations of leaves, stems and roots of three cold-temperate tree species (Abies balsamea (L.) Mill, Acer rubrum L. and Pinus strobus L.) in a forest understory. We sampled individuals varying in age (6 to 24 years old) and in size (from 2 to 500 g dry mass), and growing across a range of irradiances (from 1 to 13% of full sun) in northern Minnesota, USA. Within each species, we found small changes in R(d), N and TNC when comparing plants growing across this range of light availability. Consistent with our hypotheses, as plants grew larger, whole-plant N and TNC concentrations in all species declined as a result of a combination of changes in tissue N and shifts in biomass distribution patterns. However, contrary to our hypotheses, whole-plant and tissue R(d) increased with plant size in the three species.  相似文献   

Molecular and serological detection of Theileria equi and Babesia caballi in donkeys (Equus asinus) in Brazil     

Machado RZ  Toledo CZ  Teixeira MC  André MR  Freschi CR  Sampaio PH 《Veterinary parasitology》2012,186(3-4):461-465

Piroplasmosis in donkeys has been recognized as a serious problem of major economic importance, since the affected animals manifest loss of appetite and decreased working capacity. The present work is aimed at detecting infection or exposure of donkeys in S?o Paulo, Brazil to Theileria (T.) equi and Babesia (B.) caballi using molecular and serological approaches. EDTA-blood and serum samples were collected from 88 donkeys (Equus asinus). From 88 sampled donkeys, 65 (73.86%; 95% confidence interval, PI=63.41, 82.65) and 82 (93.2%; 95% confidence interval, PI=85.75, 97.46) animals showed IgG antibodies to T. equi (by ELISA) and B. caballi (by IFAT), respectively. Twenty-eight (31.81%; 95% confidence interval, PI=22.3, 42.61) and 18 (20.45%; 95% confidence interval, PI=12.6, 30.39) donkeys were positive to T. equi and B. caballi nested PCR assays, respectively. The results indicated that T. equi and B. caballi are prevalent among donkeys in Brazil.  相似文献