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At the Institute of Animal Nutrition of the Federal Agricultural Research Centre (FAL) in Braunschweig a feeding trial with 30 first lactating cows (German Holstein) was realised in 2004 for a complete lactation. All animals received a diet based on maize- and grass-silage ad libitum at the rate of 60:40 (DM-base) and concentrate depending on their milk yield.The animals were fed with energy and protein as recommended (GfE 2001) and were kept in loose housing. After calving they were split randomly into two feeding groups with 15 animals each. Group 1 was fed a concentrate according to the recommendations of the GfE (2001), whereas group 2 was offered the same concentrate with roughly the double amount of calcium, magnesium, phosphorus, copper, zinc, beta-carotene and vitamins A and E. To study the influence of the different concentrates on the serum concentrations of minerals and vitamins, blood, feed and milk samples were regularly taken and analysed in the course of lactation. Significantly higher blood concentrations were detected for phosphorus, zinc and vitamin E in group 2, but therefore a direct influence of the feeding on the blood concentrations was detected only for zinc and vitamin E. Over the whole lactation the average milk yield of group 1 was 2.6 kg/day higher and the average milk fat content was reduced about 0.6 percent points compared to group 2.  相似文献   
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The objective of this study was to examine the efficacy of a caudal epidural anaesthesia using lidocaine or xylazine in a high volume for analgesia of the flank, navel and hamstring tendon. Fourteen calves weighing 57.7 +/- 5.1 kg and 37.9 +/- 9.3 (mean +/- SEM) days old were randomly divided into two groups of seven calves each. Calves belonging to the lidocaine group were given a 2% lidocaine solution in the sacrococcygeal vertebral space epidurally at a volume of 0.4 ml/kg (8 mg/kg) body weight (BW). Animals of the xylazine group were administered an epidural anaesthesia with xylazine at a dose of 0.1 mg/kg BW, diluted with a 0.9% saline solution to a corresponding final volume of 0.4 ml/kg BW. Heart rate and respiratory rate were measured and the degree and duration of analgesia was determined by the response to a skin prick with a hypodermic needle over a period of 350 min after epidural injection. After epidural anaesthesia with lidocaine the mean heart rate increased during dorsal recumbency, whereas after xylazine both heart rate and respiratory rate decreased significantly (P < 0.05). The epidural injection of xylazine compared with lidocaine caused longer (P < 0.05) analgesia at the hamstring tendon (mean +/- SEM, 120.7 +/- 29.7 min versus 93.6 +/- 3.5 min) and at the flank (100.7 +/- 24.4 min versus 78.3 +/- 11.1 min). There were no differences in the intensity of analgesia between groups. After xylazine application analgesia at the navel was achieved for 95.0 +/- 14.1 min whereas after lidocaine injection sufficient analgesia at the navel was found in just two of seven calves for 55 and 95 min respectively. Based on above experiences, a second study was performed, in which a combination of xylazine and local anaesthetics was used and the injection volume was increased to prove the efficacy of caudal epidural anaesthesia in 15 calves (26.3 +/- 26.7 days; 57.1 +/- 19.5 kg) submitted to the clinic for regular umbilical surgery. In these cases the xylazine (0.1 mg/kg BW) was diluted with 2% lidocaine (n = 7) or 2% procaine (n = 8) to a corresponding final volume of 0.5-0.6 ml/kg BW. In all cases complete anaesthesia of the surgical area was achieved and no adverse effects were observed. Overall the high volume caudal epidural anaesthesia represents an effective, safe, cheap and easy to perform alternative for anaesthesia of the navel, flank and hamstring tendon in calves without major side effects.  相似文献   
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A 4-month old, 200 kg, grey warmblood colt presented for a firm, non painful mass on the distal medial aspect of the left third metatarsus. Excisional biopsy revealed a diagnosis of haemangiosarcoma. Equine haemangiosarcoma is uncommon and only limited reports of successful treatment are available. The prognosis for survival is therefore considered to be poor. After two separate incidences of recurrence with incomplete excision of the tumour, intralesional treatment with cisplatin without excision or debulking was performed on three separate occasions. Intralesional cisplatin injection was performed at monthly intervals for three treatments. Four years post treatment with cisplatin, the horse remained in remission. This case report describes the diagnostic and treatment challenges for successful treatment of a primary haemangiosarcoma on the distal limb of a warmblood foal using intralesional cisplatin chemotherapy.  相似文献   
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Summary This paper reviews research carried out at the Griffith Laboratory in Australia over the last decade on techniques for, and results of, observations of roots in irrigated clay soils. Our results emphasise the adaptability of root systems to rootzone conditions. Experiences with techniques for observing roots non-destructively in the field and both non-destructively and destructively in lysimeters are described. We concluded that the minirhizotron technique, applied in the field, was unreliable under our conditions. Horizontal root observation tubes were used in lysimeters to measure root length density (RLD) and to assess whether roots were clumped together or randomly distributed. Destructive sampling and measurement of RLD was used to establish a theoretical relationship between root intercept counts along the tubes and RLD. The application of image analysis to both destructive and non-destructive sampling in the lysimeters is outlined. The non-destructive lysimeter studies showed that roots were significantly clumped. Analysis of root intercept and root hole counts on the faces of sample cubes taken from the lysimeters showed root distribution was anisotropic over the whole soil profile for both safflower and wheat. There were many more roots and root holes present in the sampled soil cubes than was indicated by independent sampling for washed out RLD. Safflower appeared to have a faster turnover of roots than did wheat or maize. Lysimeters, equipped with horizontal root observation tubes, enabled studies to be made of many factors affecting root growth. Soils affect where and how fast roots grow, although there is also a strong species interaction. For example, soybean roots proliferated above a fresh water table in one soil but not in another; wheat had little tendency to proliferate above the water table in either soil. In wet soils, roots cease to grow once soil oxygen levels decrease below 10 mg O2 l soil -1 . This level should form the basis for soil drainage criteria. In drying soils, roots will grow successively into soil regions containing soil water: the level of adaptation being determined by soil conditions, crop growth stage and level of evaporative demand. The methods of root observation used in our studies have given quantitative assessment of root distribution. However, further research is needed to link horizontal and vertical root distribution and root adaptation more strongly to crop development and soil conditions.  相似文献   
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Summary Different soils are known to affect the amount and distribution of both available water and roots. Optimising irrigation water use, especially when shallow water-tables are present requires accurate knowledge of the root zone dynamics. This study was conducted to determine the effect of two soil types on root growth, soil water extraction patterns, and contributions of a water-table to crop evaporation (E). Two weighing lysimeters (L1 and L2) with undisturbed blocks of soil were used. The soil in L1 had higher hydraulic conductivity and lower bulk density than that in L2. Well watered conditions were maintained by irrigation for the first 110 days from sowing (DFS). Root length density (RLD) was calculated from observations made in clear acrylic tubes installed into the sides of the lysimeters. Volumetric soil water contents were measured with a neutron probe. A water-table (EC = 0.01 S m-1) was established 1 m below the soil surface 18 DFS. RLD values were greater in L1 than L2 at any depth. In L1, maximum RLD values (3 × 104 m m-3) were measured immediately above the water-table at physiological maturity (133 DFS). In L2, maximum RLD values (1.5 × 104 m m-3) were measured at 0.42 m on 120 DFS and few roots were present above the water-table. From 71 to 74 DFS, 55 and 64% of E was extracted from above 0.2 m for L1 and L2, respectively. In L2, extraction was essentially limited to the upper 0.4 m, while L1 extraction was to 0.8 m depth. Around 100 DFS the water-table contributed 29% (L1) and 7% (L2) of the water evaporated. This proportion increased rapidly as the upper soil layers dried following the last substantial irrigation 106 DFS. Over the whole season the water-table contributed 24% in L1 and 6.5% in L2 of total E.  相似文献   
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Dwarf shrub litter is thought to be of poor quality and palatability for macro-decomposers. In laboratory feeding experiments, however, it was found that this litter type represents a potential food source for earthworms and millipedes. Here we tested whether this is true under natural conditions by conducting a 1 year field experiment on an abandoned alpine pasture in Tyrol (Austria). As the natural isotopic signatures of plant litter do not allow discriminating between different plant species, dwarf shrub litter, artificially enriched in 15N, was offered in experimental plots to the macro-decomposer community. Both the earthworm Lumbricus rubellus and the millipede Cylindrojulus fulviceps fed on dwarf shrub litter as indicated by their increased δ15N signatures, but IsoError analysis indicated a clear preference for grass litter for all macro-decomposer species investigated.Soil δ15N signatures were only marginally affected by the experimental provision of 15N-enriched dwarf shrub litter, whereas litter from other, unlabelled, plants became enriched by the isotopic tracer to some extent. Except for one grass species, plants harvested at the end of the experiment were not enriched in 15N, suggesting that the N-uptake from decomposing litter material by plants on abandoned alpine pastureland is slow.  相似文献   
20.
A disorder of unknown aetiology appearing as silvering on squash (Cucurbita pepo) leaves has become prevalent in south Florida. Typical symptoms, observed after 2 weeks of exposure to adults of Bemisia tabaci , the sweet potato whitefly (SPWF) include vein clearing followed by silvering of whole leaves. Fruits from affected plants are blanched or streaked longitudinally. The silverleaf syndrome was not mechanically transmissible when crude extracts of symptomatic leaves were used as inoculum. Two sizes of double-stranded RNA (dsRNA) of c. 4.6 and 4.2 kilobase pairs (kbp) were consistently observed in leaf extracts of caged, whitefly-infested, symptomatic plants. In contrast, caged plants not exposed to whiteflies showed no symptoms and contained no detectable dsRN A. In addition, squash plants exposed to SPWF colonies from California neither developed silverleaf symptoms nor contained any dsRNA. Double-stranded RNA could also be readily detected in whitefly-infested yellow summer squash and zucchini from the field. However, asymptomatic eggplant and watermelon infested with SPWF from nearby fields did not contain detectable dsRNA. Dot-spot hybridization assays using an RNA-specific probe detected homologous sequences in both adults and nymphs of SPWF that induced silverleaf symptoms but not in adults and nymphs of SPWF that could not induce silverleaf symptoms. These results, along with the non-endogenous nature of the detected dsRNA suggest that the dsRNA is or is associated with the causal agent of whitefly-mediated leaf silvering in squash.  相似文献   
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