RNAi-mediated knockdown of INHBB increases apoptosis and inhibits steroidogenesis in mouse granulosa cells     

Mohamed M’BAYE  Guohua HUA  Hamid Ali KHAN  Liguo YANG 《The Journal of reproduction and development》2015,61(5):391-397

Inhibins are members of the TGFβ superfamily and act as suppressors of follicle stimulating hormone (FSH) secretion from pituitary glands via a negative feedback mechanism to regulate folliculogenesis. In this study, the INHBB gene was knocked down by three RNAi-Ready pSIREN-RetroQ-ZsGreen vector- mediated recombinant plasmids to explore the effects of INHBB silencing on granulosa cell (GC) cell cycle, apoptosis and steroid production in vitro. Quantitative real-time polymerase chain reaction, Western blot, flow cytometry and ELISA were performed to evaluate the role of INHBB in the mouse GC cell cycle, apoptosis and steroid production in vitro. The results showed that the relative mRNA and protein expression of INHBB in mouse GCs can be significantly reduced by RNAi with pshRNA-B1, pshRNA-B2 and pshRNA-B3 plasmids, with pshRNA-B3 having the best knockdown efficiency. Downregulation of the expression of INHBB significantly arrests cells in the G1 phase of the cell cycle and increases the apoptosis rate in GCs. This was further confirmed by downregulation of the protein expressions of Cyclin D1, Cyclin E and Bcl2, while the protein expression of Bax was upregulated. In addition, specific downregulation of INHBB markedly decreased the concentration of estradiol and progesterone, which was further validated by the decrease in the mRNA levels of CYP19A1and CYP11A1. These findings suggest that inhibin βB is important in the regulation of apoptosis and cell cycle progression in granulosa cells. Furthermore, the inhibin βB subunit has a role in the regulation of steroid hormone biosynthesis. Evidence is accumulating to support the concept that inhibin βB is physiologically essential for early folliculogenesis in the mouse.  相似文献   

Effect of Mediterranean saltbush (Atriplex halimus) ensilaging with two developed enzyme cocktails on feed intake,nutrient digestibility and ruminal fermentation in sheep          下载免费PDF全文

Haidy Alsersy  Abdelfattah Z. M. Salem  Borhami E. Borhami  Jaime Olivares  Hany M. Gado  Maria D. Mariezcurrena  Mohamed H. Yacuot  Ahmed E. Kholif  Mounir El‐Adawy  Saul R. Hernandez 《Animal Science Journal》2015,86(1):51-58

The aim of this study was to assess the effects of feeding Atriplex halimus (AH) silage treated with two developed enzyme cocktails to sheep on feed intake, nutrient digestibility and ruminal fermentation. The AH silage was treated without or with 2 L of ZAD1^® or ZAD2^®/1000 kg with 5% molasses and ensiled for 30 days. Barley grain (300 g/head/day) was fed as an energy supplement once daily at 10.00 hours and AH silage with or without enzyme treatment was offered ad libitum to animals twice daily at 09.00 and 16.00 hours. Sheep were fed on four experimental forage diets comprised of AH silage and barley (D1), AH silage treated with ZAD1^® and barley (D2), AH silage treated with ZAD2^® and barley (D3) and AH silage treated with a combination of ZAD1^® and ZAD2^® (1:1) and barley (D4). Ensiling AH with enzymes reduced its contents of neutral detergent fiber and acid detergent fiber. The dry matter intake of AH of D2, D3 and D4 decreased (P < 0.001) as compared to D1. However, enzyme‐treated diets had greater total digestible nutrients intake (P < 0.001) as compared to D1. The nutrients digestibility for D2, D3 and D4 were higher than those for D1 (P < 0.001), and were higher for D3 as compared to both D2 and D4. Sheep fed on D3 had highest (P < 0.001) ruminal total volatile fatty acids concentration, ammonia nitrogen concentration and microbial protein yield. It could be concluded that AH silage treated with ZAD1^® or ZAD2^® improved digestibility and rumen fermentation in sheep.  相似文献   

Effect of gamma and e-beam radiation on the essential oils of Thymus vulgaris thymoliferum, Eucalyptus radiata, and Lavandula angustifolia     

Haddad M  Herent MF  Tilquin B  Quetin-Leclercq J 《Journal of agricultural and food chemistry》2007,55(15):6082-6086

The microbiological contamination of raw plant materials is common and may be adequately reduced by radiation processing. This study evaluated the effects of gamma- and e-beam ionizing radiations (25 kGy) on three plants used as food or as medicinal products (Thymus vulgaris L., Eucalyptus radiata D.C., and Lavandula angustifolia Mill.) as well as their effects on extracted or commercial essential oils and pure standard samples. Comparison between irradiated and nonirradiated samples was performed by GC/FID and GC/MS. At the studied doses, gamma and e-beam ionizing radiation did not induce any detectable qualitative or quantitative significant changes in the contents and yields of essential oils immediately after ionizing radiation of plants or commercial essential oils and standards. As the maximum dose tested (25 kGy) is a sterilizing dose (much higher than doses used for decontamination of vegetable drugs), it is likely that even decontamination with lower doses will not modify yields or composition of essential oils of these three plants.  相似文献   

Integrated pest management of Tuta absoluta: practical implementations across different world regions     

Desneux  Nicolas  Han  Peng  Mansour  Ramzi  Arn&#;  Judit  Br&#;vault  Thierry  Campos  Mateus R.  Chailleux  Anais  Guedes  Raul N. C.  Karimi  Javad  Konan  Kouassi Arthur J.  Lavoir  Anne-violette  Luna  Mar&#;a G.  Perez-Hedo  Meritxell  Urbaneja  Alberto  Verheggen  Fran&#;ois J.  Zappal&#;  Lucia  Abbes  Khaled  Ali  Abid  Bayram  Yunus  Cantor  Fernando  Cuthbertson  Andrew G. S.  De Vis  Raf  Erler  Fedai  Firake  Dnyaneshwar M.  Haddi  Khalid  Hajjar  M. Jamal  Ismoilov  Khasan  Jaworski  Coline C.  Kenis  Marc  Liu  Hao-tian  Madadi  Hossein  Martin  Thibaud  Mazih  Ahmed  J. Messelink  Gerben  Mohamed  Samira A.  Nofemela  Robert S.  Oke  Abiola  Ramos  C&#;sar  Ricupero  Michele  Roditakis  Emmanouil  Shashank  Pathour R.  Wan  Fang-Hao  Wang  Ming-hui  Wang  Su  Zhang  Yi-Bo  Biondi  Antonio 《Journal of pest science》2022,95(1):17-39

Journal of Pest Science - The South American tomato pinworm, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae), has invaded most Afro-Eurasian countries and is threatening worldwide tomato...  相似文献   

The parasitoid Dolichogenidea gelechiidivoris eavesdrops on semiochemicals from its host Tuta absoluta and tomato     

Ayelo  Pascal M.  Mohamed  Samira A.  Chailleux  Ana&#;s  Yusuf  Abdullahi A.  Pirk  Christian W. W.  Deletre  Emilie 《Journal of pest science》2022,95(2):633-652

Journal of Pest Science - Semiochemicals such as herbivore-induced plant volatiles (HIPVs) and host chemicals serve as communication signals for parasitoids searching for oviposition sites. The...  相似文献   

Microscopic examination of the intestinal wall and selected organs of minipigs orally supplemented with humic acids     

Kirsten Büsing  Mohamed Elhensheri  Kristin Entzian  Udo Meyer  Annette Zeyner 《Research in veterinary science》2014

Humic acids are used to prophylactically treat intestinal diseases in a wide number of species, yet the mechanism of action remains unknown. The general assumption has been that humic acids act locally; however studies using young piglets show orally supplemented humic acids can penetrate the intestinal wall, and thus potentially act systemically. The objective of this study was to determine if humic acids could also cross the intestinal barrier in adult pigs and be detected in other organs. Adult minipigs (>18 months old) orally received either 1 g humic acids/kg body weight (verum, n = 3) or placebo (control, n = 3), for 2 weeks. At the end of the feeding period tissue samples were harvested from the intestine, various glands and organs. Unstained tissue samples were examined by light microscopy for the presence of humic acid particles. No humic acid particles were detected in any of the unstained tissues from verum or control pigs.  相似文献   

Marketing of meat sheep with intensive finishing in southern state of Mexico     

Jaime Mondragón-Ancelmo  Juvencio Hernández-Martínez  Samuel Rebollar-Rebollar  Abdelfattah Zeidan Mohamed Salem  Rolando Rojo-Rubio  Ignacio Arturo Domínguez-Vara  Anastacio García-Martínez 《Tropical animal health and production》2014,46(8):1427-1433

The objective of the present study was to quantify the marketing margins of cold sheep carcass and barbacoa meat in the southern state of Mexico. Data were obtained from the production chain links (production, industrialization, and marketing) where the marketing margins were calculated along with the benefit/cost (B/C) ratio. In the absolute marketing margin of the final consumer price per kilogram of carcass meat, the producer obtained US$2.7/kg (47 %) of the utilities, while the intermediaries obtained US$3.1/kg (53 %). Considering the final cooked product in barbacoa (typical dish), the margin was US$6.3/kg (29 %) for the producer and US$15.2/kg (71 %) for the intermediaries. The B/C ratio was 1.0, 1.1, 2, and 1.3 for the producer, stocker, butcher, and barbacoa seller, respectively. It is concluded that the best marketing channel for the producer was the producer-stocker-processor (butcher and barbacoa seller). The highest marketing margin was for the intermediaries followed by the producer. The order of importance of the B/C kilogram ratio of meat was for the butcher first, then barbacoa seller, and lastly stocker and producer.  相似文献   

Putative human and avian risk factors for avian influenza virus infections in backyard poultry in Egypt     

Basma M. Sheta  Trevon L. Fuller  Brenda Larison  Kevin Y. Njabo  Ahmed Samy Ahmed  Ryan Harrigan  Anthony Chasar  Soad Abdel Aziz  Abdel-Aziz A. Khidr  Mohamed M. Elbokl  Lotfy Z. Habbak  Thomas B. Smith 《Veterinary microbiology》2014

Highly pathogenic influenza A virus subtype H5N1 causes significant poultry mortality in the six countries where it is endemic and can also infect humans. Egypt has reported the third highest number of poultry outbreaks (n = 1084) globally. The objective of this cross-sectional study was to identify putative risk factors for H5N1 infections in backyard poultry in 16 villages in Damietta, El Gharbia, Fayoum, and Menofia governorates from 2010–2012. Cloacal and tracheal swabs and serum samples from domestic (n = 1242) and wild birds (n = 807) were tested for H5N1 via RT-PCR and hemagglutination inhibition, respectively. We measured poultry rearing practices with questionnaires (n = 306 households) and contact rates among domestic and wild bird species with scan sampling. Domestic birds (chickens, ducks, and geese, n = 51) in three governorates tested positive for H5N1 by PCR or serology. A regression model identified a significant correlation between H5N1 in poultry and the practice of disposing of dead poultry and poultry feces in the garbage (F = 15.7, p < 0.0001). In addition, contact between domestic and wild birds was more frequent in villages where we detected H5N1 in backyard flocks (F = 29.5, p < 0.0001).  相似文献   

Clodronate treatment significantly depletes macrophages in chickens     

Amber M. Kameka  Siamak Haddadi  Fathima Jesreen Jamaldeen  Prima Moinul  Xiao T. He  Fathima Hafsa P. Nawazdeen  Stephan Bonfield  Shayan Sharif  Nico van Rooijen  Mohamed Faizal Abdul-Careem 《Canadian journal of veterinary research》2014,78(4):274-282

Macrophages function as phagocytes and antigen-presenting cells in the body. As has been demonstrated in mammals, administration of clodronate [dichloromethylene bisphosphonate (Cl₂MBP)] encapsulated liposomes results in depletion of macrophages. Although this compound has been used in chickens, its effectiveness in depleting macrophages has yet to be fully determined. Here, we show that a single administration of clodronate liposomes to chickens results in a significant depletion of macrophages within the spleen and lungs of chickens up to 4 d post-treatment. This finding suggests that, in order to obtain depletion of macrophages in chickens for greater than 5 d, it is necessary to administer clodronate liposomes 4 d apart. The study also showed that 2 treatments of clodronate liposomes at 4-day intervals resulted in the depletion of macrophages for up to 10 d. The findings of the present study will encourage more precise studies to be done on the potential roles of macrophages in immune responses and in the pathogenesis of microbial infections in chickens.  相似文献   

Fungal inoculation induces agarwood in young Aquilaria malaccensis trees in the nursery   总被引：2，自引：0，他引：2  

Rozi Mohamed  Phai Lee Jong  Abd Kudus Kamziah 《林业研究》2014,25(1):201-204

Fungi are often used to induce agarwood inAquilariatrees. This study was conducted to evaluate the effect of several fungi on agarwood formation over time in youngAquilaria malaccensis （Lam.） trees. Typical changes in the length and light intensity of the resulting discoloration were observed after three and six month periods following inoculation. Wood samples were observed microscopically and classified into several light intensity groups. The discoloration length was meas-ured longitudinally. The duration after inoculation affected the mean of discoloration length： the 6-month old sample （1.70 cm） had a wider discoloration zone when compared to the 3-month old sample （1.17 cm）. When measuring the discoloration intensity, a positive relationship with time was perceived. Digital images, captured using a camera-equipped microscope, revealed that wood samples collected after six months appeared to be 1.8-times darker than after three months. We concluded that time, not the species of any of the tested fungi, had significant effect on discoloration length and intensity. Gas chromatography/mass spec-trometry （GCMS） analysis of the 6-month old sample yielded some important agarwood compounds such as benzylacetone, anisylacetone, guaiene and palustrol. This demonstrates that the tested fungi have the ability to induce agarwood formation in nurseryA. malaccensis trees.  相似文献