Drug Residues after Intravenous Anesthesia and Intrathecal Lidocaine Hydrochloride Euthanasia in Horses     

M. Aleman  E. Davis  H. Knych  A. Guedes  F. Smith  J.E. Madigan 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2016,30(4):1322-1326

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Characterization of a plasmid-encoded adhesin of an avian pathogenic Escherichia coli (APEC) strain isolated from a case of swollen head syndrome (SHS)     

Stehling EG  Yano T  Brocchi M  da Silveira WD 《Veterinary microbiology》2003,95(1-2):111-120

An avian pathogenic Escherichia coli (APEC) strain designated SHS4, isolated from a chicken with clinical signs of swollen head syndrome (SHS), adhered to but did not invade Hep-2 and tracheal epithelial cells. The PCR amplified fimA, csgA and tsh gene sequences. It produced Ia, Ib, E1, E3, K, and B colicins, but not colicin V and aerobactin. It harboured two plasmids of 60 and 98MDa and was resistant to streptomycin and tetracycline. Conjugation with a nalidixic acid (Na) resistant K-12 recipient strain (MS101) showed that the 98MDa plasmid did not transfer, whereas transfer of the 60MDa plasmid resulted in concomitant transfer of adhesion to Hep-2 and tracheal epithelial cells, production of the colicins Ia, E1, E3, and K, and the tsh-related DNA sequence. Transposon (TnphoA) mutagenesis of strain TR4 gave rise to strain Mut23, which lost its adhesive capacities, but was still able to express the same colicins as did strain TR4. PCR was able to amplify the tsh-related DNA sequence in this strain and a molecular probe based on transposon TnphoA indicated that the transposon was inserted in the 60MDa plasmid. Based on these results, we suggest that the 60MDa plasmid have adhesion genes, which may be responsible for the initial colonization of the upper respiratory tract of chickens.  相似文献   

Milk production,intake, digestion,blood parameters,and ingestive behavior of cows supplemented with by-products from the biodiesel industry     

Neto  Severino Gonzaga  Oliveira  Ronaldo Lopes  de Lima  Francisco Helton Sá  de Medeiros  Ariosvaldo Nunes  Bezerra  Leilson Rocha  Viégas  Júlio  do Nascimento  Nilton Guedes  de Freitas Neto  Marcondes Dias 《Tropical animal health and production》2015,47(1):191-200

This study aimed to evaluate the intake, digestion, blood parameters, and feeding behavior of crossbred dairy cows (Holstein?×?Gir) managed on Panicum maximum Jacq. cv. Tanzania-1 and provided supplementation with groundnut cake, sunflower cake, or palm kernel cake (to replace soybean meal). Sixteen cows were randomly assigned in a Latin square design with four treatments and four experimental periods. The consumption of nutrients from the pasture did not vary between experimental treatments. Cows receiving the palm kernel cake supplement had a reduced crude protein, non-fibrous carbohydrate, and total digestible nutrient intake and an increase in the average consumption of ether extract. There was also a reduction in the digestibility of dry matter. The inclusion of supplements in the diet did not influence the average time spent eating, ruminating, or resting. The mean values of respiratory and heart rates showed thermal comfort during the trial period. There was a reduction in blood urea nitrogen with palm kernel cake supplementation, and the values of total protein, albumin, and glucose were also significantly different with this supplement. It is recommended that cakes of groundnut cake and sunflower cake seed be used for a total replacement of soybean meal supplements for lactating cows, but the use of palm kernel cake is not recommended.

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Phosphine resistance, respiration rate and fitness consequences in stored-product insects   总被引：5，自引：0，他引：5  

Pimentel MA  Faroni LR  Tótola MR  Guedes RN 《Pest management science》2007,63(9):876-881

Resistance to fumigants has been frequently reported in insect pests of stored products and is one of the obstacles in controlling these pests. The authors studied phosphine resistance and its physiological basis in adult insects of 12 populations of Tribolium castaneum (Herbst) (Tenebrionidae), ten populations of Rhyzopertha dominica (F.) (Bostrichidae) and eight populations of Oryzaephilus surinamensis L. (Silvanidae) from Brazil, and the possible existence of fitness costs associated with phosphine resistance in the absence of this fumigant. The bioassays for the detection of phosphine resistance followed the FAO standard method. The production of carbon dioxide and the instantaneous rate of population increase (r(i)) of each population of each species were correlated with their resistance ratios at the LC(50). The resistance ratio at LC(50) in T. castaneum ranged from 1.0- to 186.2-fold, in R. dominica from 2.0- to 71.0-fold and in O. surinamensis from 1.9- to 32.2-fold. Ten populations of T. castaneum, nine populations of R. dominica and seven populations of O. surinamensis were resistant to phosphine. In all three species there was significant association (P < 0.05) between respiration rate and phosphine resistance. The populations with lower carbon dioxide production showed a higher resistance ratio, suggesting that the lower respiration rate is the physiological basis of phosphine resistance by reducing the fumigant uptake in the resistant insects. Conversely, populations with higher r(i) showed lower resistance ratios, which could indicate a lower rate of reproduction of the resistant populations compared with susceptible populations. Thus, management strategies based on the interruption of phosphine fumigation may result in reestablishment of susceptibility, and shows good potential for more effective management of phosphine-resistant populations.  相似文献   

Integrated pest management of Tuta absoluta: practical implementations across different world regions     

Desneux  Nicolas  Han  Peng  Mansour  Ramzi  Arn&#;  Judit  Br&#;vault  Thierry  Campos  Mateus R.  Chailleux  Anais  Guedes  Raul N. C.  Karimi  Javad  Konan  Kouassi Arthur J.  Lavoir  Anne-violette  Luna  Mar&#;a G.  Perez-Hedo  Meritxell  Urbaneja  Alberto  Verheggen  Fran&#;ois J.  Zappal&#;  Lucia  Abbes  Khaled  Ali  Abid  Bayram  Yunus  Cantor  Fernando  Cuthbertson  Andrew G. S.  De Vis  Raf  Erler  Fedai  Firake  Dnyaneshwar M.  Haddi  Khalid  Hajjar  M. Jamal  Ismoilov  Khasan  Jaworski  Coline C.  Kenis  Marc  Liu  Hao-tian  Madadi  Hossein  Martin  Thibaud  Mazih  Ahmed  J. Messelink  Gerben  Mohamed  Samira A.  Nofemela  Robert S.  Oke  Abiola  Ramos  C&#;sar  Ricupero  Michele  Roditakis  Emmanouil  Shashank  Pathour R.  Wan  Fang-Hao  Wang  Ming-hui  Wang  Su  Zhang  Yi-Bo  Biondi  Antonio 《Journal of pest science》2022,95(1):17-39

Journal of Pest Science - The South American tomato pinworm, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae), has invaded most Afro-Eurasian countries and is threatening worldwide tomato...  相似文献   

Pharmacokinetics and physiological effects of repeated oral administrations of tramadol in horses     

A. G. P. Guedes  H. K. Knych  J. H. N. Soares  R. J. Brosnan 《Journal of veterinary pharmacology and therapeutics》2014,37(3):269-278

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Herd diagnosis of low pathogen diarrhoea in growing pigs – a pilot study     

Ken Steen Pedersen  Markku Johansen  ?ystein Angen  Sven Erik Jorsal  Jens Peter Nielsen  Tim K Jensen  Roberto Guedes  Marie St?hl  Poul B?kbo 《Irish veterinary journal》2014,67(1)

Background

The major indication for antibiotic use in Danish pigs is treatment of intestinal diseases post weaning. Clinical decisions on antibiotic batch medication are often based on inspection of diarrhoeic pools on the pen floor. In some of these treated diarrhoea outbreaks, intestinal pathogens can only be demonstrated in a small number of pigs within the treated group (low pathogen diarrhoea). Termination of antibiotic batch medication in herds suffering from such diarrhoea could potentially reduce the consumption of antibiotics in the pig industry. The objective of the present pilot study was to suggest criteria for herd diagnosis of low pathogen diarrhoea in growing pigs.Data previously collected from 20 Danish herds were used to create a case series of clinical diarrhoea outbreaks normally subjected to antibiotic treatment. In the present study, these diarrhoea outbreaks were classified as low pathogen (<15% of the pigs having bacterial intestinal disease) (n =5 outbreaks) or high pathogen (≥15% of the pigs having bacterial intestinal disease) (n =15 outbreaks). Based on the case series, different diagnostic procedures were explored, and criteria for herd diagnosis of low pathogen diarrhoea were suggested. The effect of sampling variation was explored by simulation.

Results

The diagnostic procedure with the highest combined herd-level sensitivity and specificity was qPCR testing of a pooled sample containing 20 randomly selected faecal samples. The criteria for a positive test result (high pathogen diarrhoea outbreak) were an average of 1.5 diarrhoeic faecal pools on the floor of each pen in the room under investigation and a pathogenic bacterial load ≥35,000 per gram in the faecal pool tested by qPCR. The bacterial load was the sum of Lawsonia intracellularis, Brachyspira pilosicoli and Escherichia coli F4 and F18 bacteria per gram faeces. The herd-diagnostic performance was (herd-level) diagnostic sensitivity =0.99, diagnostic specificity =0.80, positive predictive value =0.94 and negative predictive value =0.96.

Conclusions

The pilot study suggests criteria for herd diagnosis of low pathogen diarrhoea in growing pigs. The suggested criteria should now be evaluated, and the effect of terminating antibiotic batch medication in herds identified as suffering from low pathogen diarrhoea should be explored.

Electronic supplementary material

The online version of this article (doi:10.1186/2046-0481-67-24) contains supplementary material, which is available to authorized users.  相似文献   

Evaluation and clinical use of an intraoral inferior alveolar nerve block in the horse     

T. Henry  N. Pusterla  A. G. P. Guedes  F. J. M. Verstraete 《Equine veterinary journal》2014,46(6):706-710

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Sensory Evoked Potentials of the Trigeminal Nerve for the Diagnosis of Idiopathic Headshaking in a Horse     

M. Aleman  D. Rhodes  D.C. Williams  A. Guedes  J.E. Madigan 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2014,28(1):250-253

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Genomic characterization of multidrug-resistant extraintestinal pathogenic Escherichia coli isolated from grain culture soils     

Jo&#;o Pedro Rueda FURLAN  Inara Fernanda Lage GALLO  Eliana Guedes STEHLING 《土壤圈》2022,32(3):495-502

Multidrug-resistant (MDR) Escherichia coli, mainly extraintestinal pathogenic E. coli (ExPEC), has been widely reported in infections worldwide. In agricultural soils, manure is a hotspot for the dissemination of antimicrobial resistance genes (ARGs) and pathogenic bacteria; however, MDR bacteria have also been reported in soils with no history of manure use. In addition, cross-resistance and co-resistance have been described as responsible for the metal-driven selection of bacteria resistant to antimicrobials. Therefore, the aim of this study was to analyze three MDR E. coli isolates obtained from Brazilian grain culture soil samples with no history of manure use by whole-genome sequencing. The MDR E. coli isolates were recovered from soils from corn and coffee fields, and presented resistance to β-lactams, quinolones, aminoglycosides, tetracyclines, sulphonamides, and dihydrofolate reductase inhibitor. Resistome analysis showed ARGs to several antimicrobials (i.e., β-lactams, tetracyclines, aminoglycosides, sulphonamides, trimethoprim, phenicols, fosfomycin, and macrolides) as well as several metal resistance genes and antibacterial biocide resistance genes. In addition, known mutations in quinolone-resistance-determining regions of GyrA (Ser83Leu and Asp87Asn), ParE (Ser458Thr), and ParC (Ser80Ile) were also detected. Virulome analysis showed the presence of virulence genes (lpfA, mcmA, gad, mchF, iroN, cma, and iss) associated with ExPEC. Multidrug-resistant ExPEC isolates were assigned to phylogenetic group B1. The presence of MDR B1-ExPEC in soil samples shows the ability of these isolates to survive in soils. This study reports for the first time some sequence types (i.e., ST345, ST448, and ST1146) of MDR E. coli in Brazilian soils. Therefore, these findings contribute to the monitoring of antimicrobial resistance and surveillance studies based on whole-genome sequencing worldwide.  相似文献