An 8bp indel in exon 1 of Ghrelin gene associated with chicken growth   总被引：7，自引：0，他引：7  

Fang M  Nie Q  Luo C  Zhang D  Zhang X 《Domestic animal endocrinology》2007,32(3):216-225

Ghrelin, acts as the endogenous ligand for growth hormone secretagogues receptor (GHS-R), is a novel growth hormone (GH) releasing peptide with reported effects on food intake in chickens. In this study, an 8 bp indel polymorphism in exon 1 of the chicken Ghrelin (cGHRL) gene was genotyped in a F(2) designed full-sib population to analyze its associations with chicken growth and carcass traits. Later, mRNA level in the proventriculus was determined by real-time PCR to reveal the expression feature of cGHRL gene. Result showed that this 8 bp indel was significantly associated with body weight at the age of 28 days (BW28) and 56 days (BW56), eviscerated weight (EW) and leg muscle weight (LMW) (P<0.05), highly significantly associated with hatch weight (HW), BW14, 21, 35, 42, 49, 90 and body length (BL), dressed weight (DW), eviscerated weight with giblet (EWG), wing weight (WW), breast muscle weight (BMW) and head and neck weight (HNW) (P<0.01). Meanwhile, A allele (with 'CTAACCTG') was positive for chicken growth as individuals with AA genotype had the highest value of all traits. Analysis on cGhrelin mRNA level revealed that it differed significantly among individuals with three genotypes (P<0.05). Individuals with AB genotype had the highest mRNA level, whereas that of AA had the lowest one. It was concluded that this 8 bp indel of cGHRL gene was significantly associated with most body weight and body composition traits, and negative effect of endogenous Ghrelin on chicken growth were indicated by this study.  相似文献   

Nutrient addition and disturbance effects on the community composition and assembly in a desert steppe北大核心CSCD     

Qiao J.  Zuo X.  Yue P.  Wang G.  Wang J.  Wang Z. 《干旱区研究》2023,(6):958-970

The effects of short-term nutrient additions (NPKμ, 10 g·m- 2) and disturbances on the species diversity, functional traits, soil properties, and community assembly mechanisms of the herbaceous community in the Urat desert steppe were examined. A mixed linear model, principal component analysis, and null model methods were utilized and both disturbance and interaction treatments were found to significantly reduce species richness and β diversity. Short-term nutrient additions significantly reduced soil pH but increased soil electrical conductivity. Six functional traits of the dominant species (i.e. Stipa glareosa, Peganum harmala, Salsola collina, Corispermum mongolicum) and six community-weighted mean significant changes under the disturbance and nutrient addition treatments were identified. The disturbance and nutrient additions promoted the transformation of the dominant species in the community from conservative strategies to acquisitive strategies. The results of the two null models revealed that community assembly under the control tended to be a stochastic process, while the disturbance and interaction treatments tended to be deterministic processes, and that of the nutrient addition treatments tended to be weaker deterministic processes. Plant communities in the desert steppe responded and adapted to the nutrient additions and disturbances through changes in the dominant species and their crucial functional traits. © 2023 Chinese Academy of Sciences. All rights reserved.  相似文献   

Metabolic responses of tea (Camellia sinensis L.) to the insecticide thiamethoxam     

Weiting Jiao  Ping Zhang  Chuanjian Cui  Min Yan  Qing X. Li  Yongfeng Tang  Nan Zhang  Xinyi Wang  Ruyan Hou  Rimao Hua 《Pest management science》2023,79(10):3570-3580

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Effects of Bacillus amylolyticus on Rumen Dry Matter Digestibility,Fermentation Parameters and Metabolites of Dairy Cows北大核心CSCD     

李玉琴黄新高鹏翔赵小博王慧蒋林树 《动物营养学报》2023,(1):311-322

This experiment was conducted to study the effects of Bacillus amylolyticus on rumen dry matter digestibility, fermentation parameters and metabolites of dairy cows. Experiment 1: six dairy cows with permanent fistula and had the similar parity (2 to 3 fetuses), body weight [(662 ±57) kg], lactation days [(160 ± 22) d] and milk yield [(36.1 ±3.8) kg / d], were randomly divided into 3 groups with 2 cows per group. Using 3 ×3 Latin square design, each group was extra fed 0 (control group), 5 ×109 and 5 ×1010 CFU / d Bacillus amylolyticus, the trial period was 3 periods, 13 days for each period, including 10 days for the pre-trial period and 3 days for the trial period. At the beginning of the trial period, the rumen dry matter digestibility of cows was measured by nylon bag method. Experiment 2: thirty dairy cows with similar parity [(2.5 ±0.3) fetuses], initial body weight [(559.2 ± 7. 4) kg], milk yield [(35. 2 ± 1. 5) kg / d] and lactation days [(99 ± 22) d] were randomly divided into three groups with 10 cows in each group. Cows in the 3 groups were extra fed with 0 (control group), 5 ×109 and 5 ×1010 CFU / d Bacillus amylolyticus, respectively. The pre-trial period was 10 days and the trial period was 42 days. The rumen fluid was collected on the last day of the trial period, and the rumen fermentation parameters and metabolites were determined. The results showed as follows: compared with the control group, 1) supplementary feeding of 5 × 1010 CFU / d Bacillus amylolyticus could significantly improve the rumen dry matter digestibility of dairy cows at 12, 24, 36, 48 and 72 h (P <0.05), supplementary feeding of 5 ×109 CFU / d Bacillus amylolyticus could significantly improve the rumen dry matter digestibility of dairy cows at 36 and 48 h (P <0.05); 2) supplementary feeding of 5 ×109 CFU / d Bacillus amylolyticus could significantly increase the contents of isobutyric acid, butyric acid, isovaleric acid, valeric acid, total volatile fatty acid and microbial protein in the rumen of dairy cows (P < 0. 05), supplementary feeding of 5 × 1010 CFU / d Bacillus amylolyticus could significantly increase the contents of isobutyric acid and isovaleric acid in the rumen of dairy cows and significantly reduce the rumen acetic acid / propionic acid (P < 0. 05), supplementary feeding of 5 ×109 and 5 ×1010 Bacillus amylolyticus had no effects on rumen pH and ammonia nitrogen content (P >0.05); 3) eleven metabolic pathways related to differential metabolites were detected, and ascorbic acid and alginate metabolism were significantly enriched (P < 0. 05) . In conclusion, Bacillus amylolyticus can increase the rumen dry matter digestibility, and improve the rumen fermentation function of dairy cows by affecting the content of rumen metabolites. By comprehensive analysis, supplementary feeding of 5 × 109 CFU / d Bacillus amylolyticus can achieve better effect. © 2023 Authors. All rights reserved.  相似文献   

Effects of Compound Plant Nutrients on Rumen Fermentation Parameters,Rumen Microbiota and Fatty Acid Composition of Longissimus dorsi Muscle in Finishing Small Tail Han Sheep北大核心CSCD     

王园  王文文  杨毅  高畅  郭焘  段婷  刘娜  安晓萍  齐景伟 《动物营养学报》2023,(1):380-390

This study was conducted to investigate the effects of compound p1ant nutrients (CPN) on rumen fermentation parameters, rumen microbiota and fatty acid composition of longissimus dorsi musc1e in finishing sma11 tai1 Han sheep. Sixteen 4-month-o1d finishing sma11 tai1 Han sheep with an initia1 body weight (BW) of (24.18±0.31) kg were random1y divided into two groups, name1y, with 8 rep1icates per group and 1 sheep per rep1icate. The sheep in the contro1 group were fed a basa1 diet, whereas the sheep of the contro1 group (CPN group) was fed the basa1 diet supp1ementation with 3‰ CPN. The experiment 1asted for 97 days after 7 days adaption. The resu1ts showed as fo11ows: compared with the contro1 group, 1) adding CPN decreased the concentration of ammonia nitrogen (NH3-N) (P<0.05); 2) CPN supp1ementation affected beta diversity of rumen microbiota; 3) the re1ative abundance of Acidobacteriota, Erysipe1otrichaceae_UCG-002, Lactobacillus, and Megasphaera were enhanced by adding CPN (P<0.05), whereas, the re1ative abundance of Bacteroidetes and Prevotella was 1ower (P<0.05); 4) the supp1ementation of CPN had no significant effect on fatty acid composition of longissimus dorsi musc1e of fattening sma11-tai1ed Han sheep (P>0.05); 5) the contents of tota1 MUFA, C18:1n9c, C14:1, C16:1, C18:2n6c, C18:3n3 and n-3PUFA in the longissimus dorsi musc1e were corre1ated with the re1ative abundance of Megasphaera, Erysipe1otrichaceae_UCG-002, Succiniclasticum and Ruminococcus (P<0.05). In conc1usion, CPN can regu1ate the rumen microbiota structure and reduce the rumen NH3-N concentration of fattening sma11-tai1ed Han sheep. In production practice, CPN can be used as a rumen eco1ogica1 regu1ator. © 2023 Authors. All rights reserved.  相似文献   

山东省沂南县动物强制免疫“先打后补”现状分析     

高凤华  马荣正  赵万兵  屈金涛  聂伟  李安宁  黄新强  谢有莲  王学新  宋善友  张世强 《中国动物检疫》2023,40(1):68-71

2021年,沂南县推进动物疫病强制免疫“先打后补”工作,依托“鲁牧云”信息平台,通过强化组织领导,加强政策宣贯,强化人员培训,放开强免疫苗经营渠道,提供抗体检测无偿服务等措施,提升了养殖场户“先打后补”的积极性、自主性、灵活性,强化了养殖场户动物防疫主体责任,满足了养殖场户疫苗多样化需求。但由于 “先打后补”补助金额少、社会化服务不规范、基层动物防疫体系弱化、缺少配套工作经费等原因,部分养殖场户实施“先打后补”的积极性不高。建议通过规范社会化服务组织,强化培训水平,提高补助标准,强化三级（县乡村）基础设施建设,升级“鲁牧云”等措施,确保“先打后补”政策惠及更多养殖场户。  相似文献   

盐胁迫下5-磺基水杨酸、水杨酸对小黑麦种子生理特性的影响   总被引：6，自引：0，他引：6  

李焰焰  聂传朋  董召荣 《种子》2005,24(1):8-10,72

采用种子萌发试验,研究了5-磺基水杨酸、水杨酸对盐胁迫下小黑麦种子萌发及幼苗生长情况的影响.结果表明:在盐胁迫下,适当浓度的两种酚酸均能提高小黑麦种子萌发的发芽率、发芽指数、活力指数,浓度增高则抑制种子萌发.适当浓度的两种酚酸还能降低幼苗叶片电导率和丙二醛(MDA)含量、提高叶绿素含量.总的看来,5-磺基水杨酸的效果比水杨酸好,且低浓度的效果比高浓度的好.  相似文献   

Design method and homogeneity control of bridge fair-faced concrete     

Zhou Xiaojun  Mou Tingmin  Ding Qingjun  Nie Chuanzhen 《保鲜与加工》2015,(1):117-122

According to the structural characteristics of bridge engineering, the mix optimization design method of bridge fair-faced concrete was presented, and C30~C50 concrete with high cohesion and package, outstanding fluidity and excellent mechanical properties were prepared. Through the test of air content of fresh concrete, micro hardness and micro structure in different parts of hardened concrete specimens, effects of viscosity-modifying admixtures on homogeneity of concrete were discussed. The results showed that the composition of bridge fair-faced concrete were relatively homogeneous, and the microstructure were relatively dense. When C30 concrete mixed with 5 × 10 -5 cellulose ether or 6% silica fume (mass fraction of cementitious material), air content of fresh concrete were less than 2%, and slurry viscosity values were between 1 900 and 2 000 MPa·s. Also, the micro hardness values in different parts of hardened concrete specimens were closed. The results were applied in practical bridge engineering, which generally meets the apparent quality requirements of bridge fair faced concrete.  相似文献   

A Dynamic Model for Generation Expansion Planning and Algorithm     

Yang Xiutai Nie Duan 《保鲜与加工》1993,(4):6-10

This paper discusses the problem of long-range generation expansion planning. A dynamic model for long-range expansion planning is set up and an algorithm-a two-step programming is presented, which is used here in a case.  相似文献   

城市垃圾填埋及沼气收集利用   总被引：17，自引：1，他引：17  

聂永丰  张秀蓉 《中国沼气》1997,15(2):17-20

本文针对目前我国日益严重的城市固体废物污染环境的问题，在对此填埋，焚烧和高温堆肥等处理处置方法的基础上，分析了填埋产沼技术的可行性，指出其兼有的废物处理与处置的双重性，具有处理量大、处理费用低、可还原利用上地、能回收能源等多种优点，在我国有广阔应用前景。  相似文献