Babesia odocoilei infection in elk     

Gallatin LL  Irizarry-Rovira AR  Renninger ML  Holman PJ  Wagner GG  Sojka JE  Christian JA 《Journal of the American Veterinary Medical Association》2003,223(7):1027-32, 986

Two male North American elk from a commercial herd were evaluated because of a sudden onset of lethargy, anorexia, and voiding of red urine. These 2 elk were kept in the same pen as 4 other male elk that had died during the preceding 2 months. Laboratory analyses revealed anemia and intraerythrocytic parasites, later confirmed as Babesia odocoilei (a protozoal hemoparasite of cervids). Of the 240 elk remaining in the herd, 59 were screened for B odocoilei by microscopic evaluation of blood smears, protozoal culture of blood, and immunofluorescent antibody testing of serum. Of those 59 elk, 34 (58%) were infected with B odocoilei. Babesia odocoilei infection in elk can be fatal and should be considered in cases of sudden death or acute hemolytic anemia. Familiarity with the disease in elk is essential for practitioners because of the increasing popularity of commercial elk farming.  相似文献   

Intravascular lymphoma involving the central and peripheral nervous systems in a dog     

Bush WW  Throop JL  McManus PM  Kapatkin AS  Vite CH  Van Winkle TJ 《Journal of the American Animal Hospital Association》2003,39(1):90-96

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Jojoba seed meal proteins associated with proteolytic and protease inhibitory activities     

Shrestha MK  Peri I  Smirnoff P  Birk Y  Golan-Goldhirsh A 《Journal of agricultural and food chemistry》2002,50(20):5670-5675

The jojoba, Simmondsia chinensis, is a characteristic desert plant native to the Sonoran desert. The jojoba meal after oil extraction is rich in protein. The major jojoba proteins were albumins (79%) and globulins (21%), which have similar amino acid compositions and also showed a labile thrombin-inhibitory activity. SDS-PAGE showed two major proteins at 50 kDa and 25 kDa both in the albumins and in the globulins. The 25 kDa protein has trypsin- and chymotrypsin-inhibitory activities. In vitro digestibility of the globulins and albumins resembled that of casein and soybean protein concentrates and was increased after heat treatment. The increased digestibility achieved by boiling may be attributed to inactivation of the protease inhibitors and denaturation of proteins.  相似文献   

Elemental composition of teeth with and without odontoclastic resorption lesions in cats     

Colley PA  Verstraete FJ  Kass PH  Schiffman P 《American journal of veterinary research》2002,63(4):546-550

OBJECTIVE: To determine elemental composition of teeth with and without odontoclastic resorption lesions (ORL) in cats. SAMPLE POPULATION: Normal teeth from 22 cadaver cats and ORLaffected teeth from 21 cats admitted to the veterinary hospital for dental treatment. PROCEDURE: An electron microprobe was used to analyze weight percentages of calcium, phosphorus, fluorine, sodium, magnesium, sulfur, potassium, and iron in enamel, dentin, and cementum. RESULTS: Calcium and phosphorus were the most abundant elements. Fluorine, sodium, and magnesium combined were < 5% and sulfur, potassium, and iron combined were < 0.1% of total elemental composition. In enamel of normal teeth, a significant sex-by-jaw location interaction was seen in mean (+/- SD) phosphorus content, which was higher in mandibular teeth of females (1764+/-0.41%) but lower in mandibular teeth of males (16.71+/-0.83%). Mean iron content in dentin of normal teeth was significantly lower in mandibular teeth than maxillary teeth (0.014+/-0.005% vs 0.023+/-0.019%). Mean enamel sodium content was significantly higher (0.77+/-0.046% vs 0.74+/-0.025) and mean enamel iron content was significantly lower (0.017+/-0.008% vs 0.021+/-0.005%) in ORLaffected teeth, compared with normal teeth. In cementum, mean fluorine content was significantly lower (2.98%+/-0.27 vs 2.99+/-0.20%) and mean magnesium content was significantly lower (0.54+/-0.13% vs 0.60+/-0.13%) in ORL affected teeth, compared with normal teeth. CONCLUSIONS AND CLINICAL RELEVANCE: Results of our study establish baseline mineral content of enamel, dentin, and cementum for normal teeth in cats. Minimal differences in mineral content of enamel and cementum of normal and ORL-affected teeth were detected.  相似文献   

The modern working dog--a call for interdisciplinary collaboration     

Olson PN 《Journal of the American Veterinary Medical Association》2002,221(3):352-355

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Seroprevalence of antibodies specific for gram-negative core antigens in chickens on the basis of an Escherichia coli J5 enzyme-linked immunosorbent assay     

Ruble RP  Wakenell PS  Cullor JS 《Avian diseases》2002,46(2):453-460

Antibodies directed toward gram-negative core antigens (GNCAs) have been demonstrated in many mammalian species but to date are unexamined in any avian species. An enzyme-linked immunosorbent assay with phenol-killed whole cell Escherichia coli J5 was used to assess the presence of serum antibodies directed toward GNCAs in chickens. The first experiment consisted of collecting blood samples from randomly selected hens at egg laying ranches in northern California. The ages ranged from several days of age to 77 wk of age. Birds were classified into age groups (hatchling [1 day-4 wk], pullet [4-18 wk], pullet cycle [18-60 wk], and postmolt [>60 wk]) and husbandry style for titer comparison. The geometric mean titer (GMT) for all adult hens regardless of age was 2147. The geometric mean titers were 220, 5691, 2304, and 1776 for hatchlings, pullets, pullet cycle hens, and postmolt hens, respectively. The age group titer trends were similar to those of humans rather than those of farm animals in that the highest titers occurred during "adolescence" (pullets) and titers decreased slightly with maturity. The GMTs were 2870 for hens housed intensively and 1872 for those housed extensively. The second experiment looked at the progression of GNCA titers within individual birds over a 1-yr period. Individual titers increased slightly throughout the study time of the second experiment.  相似文献   

Vecuronium bromide,phenylephrine and atropine combinations as mydriatics in juvenile double-crested cormorants (Phalacrocorax auritus)     

Loerzel SM  Smith PJ  Howe A  Samuelson DA 《Veterinary ophthalmology》2002,5(3):149-154

Topical vecuronium bromide (Norcuron) and combinations with atropine and phenylephrine, were evaluated as mydriatics in juvenile double-crested cormorants (Phalacrocorax auritus). Nine cormorants were treated with each of four protocols: 1% atropine; 4 mg/mL vecuronium bromide (total 0.16 mg/eye); atropine with vecuronium; and atropine, 2.5% phenylephrine, followed by vecuronium. Drugs were applied topically at 15-min intervals (0.01 mL/drop). Pupil diameter was measured manually every 15 min with a pupil gauge calibrated to the nearest 0.5 mm. No effect was observed with atropine alone. Average +/- SD peak pupil diameter for vecuronium, atropine/vecuronium, and atropine/phenylephrine/vecuronium were 5.4 +/- 1.1 mm, 5.7 +/- 0.8 mm and 6.2 +/- 0.4 mm, respectively; and duration of peak diameters were 38 +/- 28 min, 79 +/- 71 min and 103 +/- 58 min, respectively. The combined atropine, phenylephrine and vecuronium provided the most consistent dilation with larger average pupil size and longer average duration. No side-effects from vecuronium were observed in these birds.  相似文献   

Glycoprotein M of bovine herpesvirus 1 (BHV-1) is nonessential for replication in cell culture and is involved in inhibition of bovine respiratory syncytial virus F protein induced syncytium formation in recombinant BHV-1 infected cells     

König P  Giesow K  Keil GM 《Veterinary microbiology》2002,86(1-2):37-49

Cell cultures infected with BHV-1/F(syn), a recombinant bovine herpesvirus 1 (BHV-1) which expresses a synthetic open reading frame encoding the fusion (F) protein of the bovine respiratory syncytial virus (BRSV), showed a cytopathic effect (CPE) indistinguishable from that induced by wildtype BHV-1 although transient transfection experiments demonstrated that expression of the F protein leads to formation of large syncytia. Since it has been shown that glycoprotein M (gM) of pseudorabies virus inhibits BRSV F-induced syncytium formation in transient plasmid transfection experiments [Pseudorbies virus glycoprotein M inhibits membrane fusion. J. Virol. 74 (2000) 6760], the gM ORF of wtBHV-1 and BHV-1/F(syn) was interrupted. Infection of cell cultures with the resulting gM(-) mutant of BHV-1/F(syn) led to formation of syncytia, whereas the CPE in gM(-)BHV-1 infected cells was comparable to the CPE in wtBHV-1 infected cultures. Our results demonstrate that gM is not essential for BHV-1 replication in cell culture and that gM is involved in inhibition of the cell fusion activity of the BHV-1 expressed BRSV F protein.  相似文献   

Application and field validation of a PCR assay for the detection of Mycoplasma hyopneumoniae from swine lung tissue samples.   总被引：1，自引：0，他引：1  

Hugh Y Cai  Tony van Dreumel  Beverly McEwen  Geoff Hornby  Patricia Bell-Rogers  Pat McRaild  Gaylan Josephson  Grant Maxie 《Journal of veterinary diagnostic investigation》2007,19(1):91-95

A PCR assay was validated for the detection of Mycoplasma hyopneumoniae in porcine lung tissue. The detection limit of the assay was 0.18 colony-forming units/g of lung sample spiked with M. hyopneumoniae. In field validation, 426 pigs from 220 cases were examined for M. hyopneumoniae infection by M. hyopneumoniae PCR and a fluorescent antibody (FA) test. In total, 103 pig lungs (24.2%) were positive in the PCR test, and 69 pig lungs (16.2%) were positive in the FA test, among which, 62 pigs were positive for both PCR and FA test. Most of the PCR-positive but FA test-negative cases had lesions compatible with M. hyopneumoniae infection. With Bayesian modeling, the diagnostic sensitivity and specificity of the PCR were determined to be 97.3% and 93.0%, respectively.  相似文献   

Oral eosinophilic granulomas in tigers (Panthera tigris)--a collection of 16 cases.     

John M Sykes  Michael M Garner  Leah L Greer  Nancy P Lung  Rob L Coke  Frank Ridgley  Mitch Bush  Richard J Montali  Ben Okimoto  Robert Schmidt  Jack L Allen  Bruce A Rideout  Patricia A Pesavento  Edward C Ramsay 《Journal of zoo and wildlife medicine》2007,38(2):300-308

Oral eosinophilic granulomas were diagnosed in 16 tigers (Panthera tigris). All lesions were located on the hard or soft palate and typically consisted of flat or slightly raised circular ulcers. Histologic features of these lesions were essentially identical to those seen in oral eosinophilic granulomas of domestic cats and dogs. No clinical signs were noted in eight cases, though various degrees of inappetence, excessive salivation, and dysphagia were noted in the other eight tigers. Six cases were not treated. Treatment for the remaining 10 cases centered on corticosteroids and additional treatments included surgical removal, cryotherapy, antibiotics, and chlorpheniramine. Treatment with corticosteroids did appear to be effective in some cases, though lesions would worsen after cessation of therapy and no cases were cured. In addition, three cases developed complications possibly related to this corticosteroid therapy. The etiology of these lesions remains unknown, though an underlying allergic condition is likely.  相似文献