客户关系管理与维护的思考     

单红艳 《湖南农机》2017,44(2)

现代企业已逐步由传统的以产品和规模为中心的粗放式经营管理模式向以客户为中心、服务至上、实现客户价值和达到企业利润最大化的集约化经营管理模式转变,良好的客户关系是企业求得生存和发展的重要资源.在企业里如何运用好客户关系管理与维护的理论与技术,对提高企业竞争力,获得更高的商业利润,改善客户关系,是从事客户关系维护相关人员应该思考的问题.  相似文献   

北方牧区种草养畜综合配套技术及其推广效益   总被引：2，自引：0，他引：2  

山薇  杨晓东  刘琳  员旭疆  李荣 《中国草地学报》2000,(2):75-78

通过对草地进行科学管理、合理利用及培育改良，建立优质高产的饲料基地，开展饲草料加工、调制、青(微)贮，采用以肉羊为主的模式化育肥技术，提高了种草养畜的整体生产水平。1996～1997年新增总产值7555.15万元，新增纯收益5037.67万元，实现了草地生态、牧业生产的良性循环，为发展草地畜牧业现代化走出了一条新路。  相似文献   

Effects of cytokines on renovation of acute renal failure in mouse with bone marrow derived mesenchymal stem cell transplantation     

WAN Jian-xin  GUO Qi  WANG Jing-han  PAN Yang-bin  CUI Jiong  FU Bin-bin  XU Yan-fang 《园艺学报》2009,25(8):1628-1634

AIM: To observe the effects of cytokines on renovation of acute renal failure (ARF) in mouse with bone marrow derived mesenchymal stem cell (MSC) transplantation. METHODS: ARF animal model was induced in mouse by subcutaneous injection of cisplatin. Mice were randomly assigned into 3 groups: normal control group, ARF group and MSC group. After 24 h cisplatin injection, animals were injected intravenously with MSC in MSC group. Animals were sacrificed at 1 d, 4 d, 7 d, 14 d and 28 d after cisplatin injection. The blood urea nitrogen (BUN) and serum creatinine (Scr) were measured. The renal morphologic changes were scored with Paller’s criterion on hematoxylin and eosin (HE) stained sections. The mRNA and protein expressions of HGF, BMP-7, TNF-α and IL-10 were detected by RT-PCR and immunohistochemistry method. RESULTS: After 4 d of cisplatin injection, the BUN and Scr values in MSC group were significantly lower than those in ARF group (P<0.01). After 7 d and 14 d, the values of BUN and Scr in MSC group were still lower than those in ARF group (P<0.01, P<0.05). The renal morphologic scores of MSC group were also lower than those of ARF group. After 7 d, the expressions of HGF, BMP-7 and IL-10 were higher in MSC group than those in ARF group, the expression of TNF-α in MSC group was lower than that in ARF group. CONCLUSION: MSC promotes the recovery of acute renal failure induced by cisplatin. The mechanism may partly depend on paracrine of growth factor and amelioration of inflammatory.  相似文献   

Research Progress Towards the Liquid Chip Technology in Detection of Animal Infectious Diseases     

DU Wenqi  XIA Liye  LI Guimei  SHAN Hu 《中国畜牧兽医》2007,47(12):4138-4147

Liquid chip technology is a novel biomolecular detection technology which integrates laser technology,flow cytometry,digital signal processing and traditional chemical technology.It is widely used in various immunological analysis and nucleic acid detection.Single and mutiplex analysis are supported,with protein and nucleic acid targets detected in a variety of detection methods in high throughput manner.It has advantages of high throughput,easy operation,wide range of application,good repeatability,high specificity,less sample needed,high sensitivity and stablility,and low cost.Therefore,they are gradually replacing the traditional detection and quantitative pathogen methods,such as Real-time fluorescent quantitative nucleic acid amplification detection system (qPCR),enzyme-linked immunosorbent assay (ELISA) and other detection methods.Animal infectious diseases seriously endanger the development of the breeding industry,futhermore,some zoonoses such as highly pathogenic avian influenza also pose a serious threat to human health.An efficient and sensitive diagnostic system will help to screen a large number of samples during the outbreak of infectious diseases and prevent the spread of infection.The development of liquid chip technology provides a new platform for high-throughput detection and disease prevention.In this review,the principle and advantages of liquid chip technology are briefly described.The research progress of liquid chip technology in the detection of animal infectious diseases,including pigs diseases,poultry diseases,rabbit diseases,dog diseases,rodent diseases and other animal infectious diseases are summarized.We believe that in the future,this technology will become an important analytical and testing tool in clinical diagnosis,basic research,new drug development,judicial identification,food health supervision,biological weapons prevention and other fields.The development of this technology will greatly promote the research and development of life science.  相似文献   

后备母猪的营养与繁殖     

单妹 《广东饲料》2007,16(6):15-16

繁殖效率的高低,除受品种和管理因素影响外,营养因素的作用不可忽视,通过改善营养水平可提高高产母猪的繁殖能力。近年来,随着集约化生产的发展,后备母猪的营养需要和饲养管理已成为动物营养研究的新热点,其中采食量及理想的营养水平作为影响其繁殖性能的重要因素,引起了广泛的关注。现就营养对后备母猪各个繁殖阶段繁殖性能的影响作一简单论述,希望对养殖生产者有一定帮助。  相似文献   

枣在泰安的主要物候期观察   总被引：1，自引：0，他引：1  

单公华  王斌  周广芳  高丽 《落叶果树》2000,32(4):10-11

调查记载了山东省果树研究所枣品种圃(泰安)近80个品种的主要物候期。观察到不同品种发芽期相差13天，展叶期、花期相差5～6天，成熟期则相差50～60天。果实成熟期早晚取决于品种遗传特性，与发芽、展叶、开花期早晚无关。此观察结果可实为引种依据。  相似文献   

不同小麦日粮对肉仔鸡肉质、脂肪酸合成酶mRNA与脂蛋白脂肪酶mRNA表达的影响   总被引：1，自引：0，他引：1  

宋凯  单安山 《动物营养学报》2008,20(1):69-74

本文旨在研究不同小麦日粮对肉仔鸡生长、肉质及脂肪酸合成酶(FAS)mRNA与脂蛋白脂肪酶(LPL)mRNA表达的影响。选用300只艾维因肉仔鸡公雏,随机分为5组,每组4个重复,每个重复15只鸡,5组分别饲喂玉米日粮和4种水溶性阿拉伯木聚糖(WSAX)含量不同的小麦日粮,试验期为6周。结果表明:①随着小麦日粮中WSAX含量的增加,肉仔鸡的生长明显降低;②小麦日粮显著增加了肌肉持水力,并随小麦WSAX含量增加而升高的趋势显著(P<0.01),剪切力也随之增加(P<0.05)。饲喂小麦日粮的肉仔鸡其肉色显著低于玉米日粮组(P<0.01),各小麦日粮组间差异不显著。当小麦WSAX含量较高时,小麦日粮可以显著降低肉仔鸡肌肉脂肪和胆固醇含量(P<0.05)。各处理间胸肌、腿肌含水量无差异(P>0.05);③小麦日粮显著降低FASmRNA在肉仔鸡肝中的表达量(P<0.01),并且随日粮WSAX含量增加而表达水平降低的趋势明显。各处理间LPLmRNA表达量的差异不显著(P>0.05)。本研究表明:①当小麦中WSAX含量较低时,对肉仔鸡增重和肉质的影响较小,是玉米理想的替代品;②当小麦中WASX含量较高,则会严重影响肉仔鸡的增重、肉质及FASmRNA在肝脏中的表达,不宜在肉仔鸡日粮中大量添加。  相似文献   

The distribution and releasing rate of methane in sediment of culture area in Rushan Bay     

WANG Juan  QU Ke-ming  XU Yong  SHAN Bao-tian 《海洋水产研究》2008,29(6)

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2株鲤鱼源乳酸菌体外抗逆性研究          下载免费PDF全文

单晓枫  孙烨林  张洪波  张喜宏  钱爱东 《水生态学杂志》2008,29(2):124-127

对2株鲤鱼源乳酸菌作为益生菌候选菌株进行体外抗逆性试验。通过耐受胆盐、耐受pH 值、耐受蛋白酶和耐高温等试验, 对这2株乳酸菌进行研究。其中在胆盐耐受性试验中, YL- 1和YL- 7在胆盐浓度0. 4%、培养4 h时仍有30%以上的存活率; 在pH 值耐受性试验时, 2株乳酸菌均可在一定的酸性和碱性条件下存活; 胰蛋白酶对2株菌的存活率没有影响; 在70e 的高温中, 2株乳酸菌均有一定的耐受性。  相似文献   

鹰形人字拱斜拉桥的设计与分析     

苏彬彬 《湖南农机》2012,(9):257-258

提出一种新型的组合桥型-鹰型人字拱斜拉桥。文章强调桥梁设计应综合考虑使用功能、力学特性。建筑艺术以及与环境的协调性。文章介绍了它的空间构成,分析了它的受力特征,论证了该结构的科学性、合理性与先进性。并从整体上阐述该结构的适用范围、时代特征及现实意义。  相似文献