Effect of concentrated decoction of Chinese herbal compound Buyanghuanwutang on cAMP-PKA-CREB signaling pathway in hippocampus of rats with vascular dementia     

ZHANG Hong-bo  GAO Wei-juan  QIAN Tao  TANG Jing-long  LI Jun 《园艺学报》2011,27(4):718-721

AIM: To evaluate the role of concentrated decoction of Chinese herbal compound Buyanghuanwutang (BYHWT) in cyclic adenosine monophosphate(cAMP)-protein kinase A(PKA)-cAMP response element-binding protein(CREB) signaling pathway in hippocampus of rats with vascular dementia (VD). METHODS: The rats were randomly divided into sham operation group (sham-operated rats treated with normal saline), VD model group (VD rats treated with normal saline), BYHWT treatment group (VD rats treated with BYHWT) and nimodipment treating group (VD rats treated with nimodipine). The rat model of VD was build by the method of four-vessel occlusion. The rats in all 4 groups were administered with the corresponding reagents for successive 30 days. The content of cAMP was measured by radioimmunoassay. The expression of PKA catalytic subunit (PKAc) was observed by Western blotting. The changes of DNA-binding activity of CREB in rat hippocampus were detected by electrophoretic mobility shift assay. RESULTS: The content of cAMP, the expression of PKAc and the DNA-bingding activity of CREB in the hippocampus of VD rats were lower than those in the hippocampus of sham-operated rats (P<0.01). The above indexes in both nimodipine treatment group and BYHWT treatment group were definitely higher than those in VD model group (P<0.01). CONCLUSION: BYHWT increases the content of cAMP, the expression of PKAc and the DNA-binding activity of CREB in VD rat hippocampus, thus strengthening the cAMP-PKA-CREB signaling pathway.  相似文献   

花椰菜—黑芥体细胞杂交获得抗黑腐病异附加系新材料   总被引：1，自引：0，他引：1  

王桂香  严红  曾兴莹  盛小光  唐宇  韩硕  宗梅  陆坤  刘凡 《园艺学报》2011,38(10):1901-1910

 以花椰菜—黑芥体细胞杂种的自交及回交后代为材料,建立了结合抗病性人工接种鉴定,流式细胞仪DNA含量分析,B基因组特异分子标记和染色体原位杂交的大规模群体分析技术,在50份高代自交及回交材料中筛选获得了20份形态学特征偏向花椰菜,30份呈花椰菜—黑芥中间类型的材料。细胞DNA含量检测（FLC）及染色体计数结果表明,偏花椰菜的类型,DNA含量基本在2.24 pg · cell^-1以下,染色体数在28条以下。经连续3年黑腐病病菌人工接种鉴定,获得高抗株系12个,抗性株系17个,占全部受试材料的14%。对其中3个（PFCN29 BC3-3-5、PFCN16-1 S2BC3-107和PFCN14-1 S1BC4-123）形态偏花椰菜且高抗黑腐病的植株进行基因组原位杂交,结果显示其分别为花椰菜染色体组附加了2、7和8条黑芥染色体的异附加系材料。这些植株可以用于后续的研究和作为育种桥梁材料,进一步获得黑腐病抗性的单体附加系或渐渗系。本研究表明通过体细胞杂交转育野生优异抗性资源,创新甘蓝类蔬菜育种种质的可行性。  相似文献   

Endogenous hydrogen sulfide regulates oxidant/antioxidant balance in rat with chronic obstructive pulmonary disease     

HE Yan-jing  CHEN Ya-hong  QI Yong-fen  YAO Wan-zhen  BI Xiao-rui  GUAN Yu-hong  ZHU Rui-xia  TANG Chao-shu 《园艺学报》2011,27(6):1169-1175

AIM: To investigate the role of hydrogen sulfide (H₂S) in the protection against oxidative stress in rats with chronic obstructive pulmonary disease (COPD).METHODS: The rat model of COPD was established by cigarette smoking (CS) combined with lipopolysaccharide (LPS) instillation. Thirty-two healthy male Sprague-Dawley rats were randomly divided into 4 groups: control group, CS+LPS group, CS+LPS+NaHS (H₂S donor) group and CS+LPS+PPG (DL-propargylglycine, an inhibitor of cystathionine-γ-lyase) group. After 30 days, the lung functions of the rats were measured, the histological changes of lungs were observed under light microscope and the pathological scores were calculated. The H₂S level in plasma and the protein expression of cystathionine-γ-lyase (CSE) in the lung tissues were measured. The content of malondialdehyde (MDA), the activity of superoxide dismutase (SOD) and catalase (CAT) were detected to reflect oxidative stress.RESULTS: Compared with control group, the peak expiratory flow (PEF) decreased by 24% and intra-pressure (IP) increased by 66% in CS+LPS group. The pathological scores of the lung tissues also increased. Compared with CS+LPS group, no change in the lung function was observed after given NaHS or PPG, but the pathological scores decreased in CS+ LPS+ NaHS group. Compared with control group, the content of H₂S in plasma was increased by 26% on day 16. Compared with CS+LPS group, the content of H₂S in plasma of CS+LPS+PPG group was decreased by 22% after 30 days. Compared with control group, the protein expression of CSE increased, and no statistical difference among CS+LPS group, CS+ LPS+ NaHS group and CS+LPS+PPG group was observed. Compared with control group, MDA content in the lung tissues was increased by 24% in CS+LPS group, the activity of SOD was increased by 47% and the activity of CAT was increased by 52%. Compared with CS+LPS group, the MDA content in CS+LPS+NaHS group was decreased by 21%, and no statistical difference in the activity of SOD and CAT was observed. The activity of SOD decreased by 33% after given PPG.CONCLUSION: H₂S plays a role as anti-oxidant in the rats with COPD. The CSE/ H₂S pathway may be involved in the development of COPD.  相似文献   

环境友好农业生产的生态补偿机制探索与实践   总被引：1，自引：0，他引：1  

唐铁朝  边艳辉  刘峰  武海燕 《农业环境与发展》2011,28(4)

围绕中德技术合作"中国华北地区集约化农业的环境战略"项目实施过程中,为鼓励农民采用节水、节肥、节药环境友好方式生产安全优质设施蔬菜,减轻农业面源污染,保护农业生态资源与环境而开展的生态补偿的实践进行论述,结合项目的实践从补偿原则、补偿对象、补偿标准、补偿形式、补偿环节、补偿监管等方面进行了探讨。笔者认为,建立健全农业生态环境补偿制度符合世界贸易组织农业协议绿箱政策,有利于推动环境友好技术的应用。环境友好农业生产的生态补偿应以项目(政府)补贴为主导,以农民自愿为前提,以骨干农户、农民专业合作社为主要补偿对象,以技物结合为主要补偿形式,以兼顾生态、经济和社会效益为目标。  相似文献   

创建高职教育高等数学动态化教学体系探讨     

唐建玉 《计算机与农业》2011,(6):139-140

通过对高等数学教学现状的分析,阐述了高等数学教学改革的必然性,论述了构建高等数学动态教学体系的要素及条件。  相似文献   

施肥模式对双季稻产量、养分吸收及经济效益的影响   总被引：4，自引：2，他引：2  

汤雷雷  万开元  李祖章  陈防 《植物营养与肥料学报》2011,17(2):259-268

水旱轮作田间定位试验条件下研究了连续4年（20052008）不同施肥模式对双季稻产量、养分吸收及经济效益的影响。结果表明,氮磷钾肥配施能显著提高水稻产量,长期有机无机肥配施增产效果明显。定位4年后,有机无机肥配施处理早稻和晚稻平均产量分别比未施肥处理增产135%和130%。随着产量的增加,稻草与稻谷中氮、磷和钾养分吸收量呈增大的趋势,表明水稻植株养分吸收量与稻谷产量呈显著正相关。不同施肥模式显著影响水稻稻草、稻谷中的氮、磷、钾养分含量。氮磷钾肥配施处理,特别是有机无机肥配施处理稻谷的养分吸收量最高,在土壤中的氮、磷盈余也最少。肥料配施虽增加了支出,但提高了经济产出,其纯收益相对较高,以有机无机肥配施处理的纯收益最高。双季稻生产实践中,有机无机肥配施模式值得推荐且需合理增施磷、钾肥。  相似文献   

氟对茶树生长、叶片生理生化指标与茶叶品质的影响   总被引：1，自引：0，他引：1  

唐茜  赵先明  杜晓  吴永胜  梁琪惠  王春梅 《植物营养与肥料学报》2011,17(1):186-194

以2个茶树品种为试验材料,通过土壤盆栽试验,研究了土壤添加氟浓度为0～200 mg/kg对茶树生长、叶片生理生化指标与茶叶品质的影响以及茶树的氟累积特性。结果表明,随氟处理浓度增加,茶树的生长、叶绿素合成和光合作用均受到明显抑制; 福鼎大白茶的SOD、CAT和POD活性均随氟处理浓度的增加呈先升高后降低趋势,名山白毫的3种酶活性则受到明显抑制。2个品种的新梢中游离氨基酸、儿茶素组分和咖啡碱含量也随施氟浓度的增加总体呈下降趋势,茶叶品质降低,表明氟可能抑制茶树儿茶素的合成代谢和氮素代谢。茶树的氟累积量也随着土壤氟水平的增加而增加,且各器官的氟含量(y)与土壤中添加的氟(x)呈显著或极显著线性正相关; 茶树的聚氟能力依次为叶片＞新梢＞枝条＞茎＞根,叶片是氟累积的主要器官。福鼎大白茶耐氟、抗氟的能力明显强于名山白毫,但氟积累能力则低于名山白毫。  相似文献   

洱海北部地区不同轮作农田氮、磷流失特性研究   总被引：7，自引：2，他引：5  

汤秋香  任天志  雷宝坤  翟丽梅  胡万里  张继宗  林涛  刘宏斌 《植物营养与肥料学报》2011,17(3):608-615

为明确洱海北部地区不同轮作模式下农田氮、磷流失特性,客观评价环境污染风险。试验在洱海北部地区沿弥苴河和罗时江流域的7个乡镇进行。采用定点取样与实地调研相结合的方法进行多点重复监测,调查不同轮作模式、轮作周期内田面水和沟渠水的氮、磷含量变化情况,研究不同轮作模式下氮、磷污染的负荷及其影响因素。结果表明,田面水中,不同轮作模式下氮素的流失差异显著且以水溶态为主,可溶性氮浓度以大蒜水稻模式最高,油菜水稻模式最低,其它轮作模式居中分布;降雨径流氮流失量以大蒜水稻模式最高。磷素流失总量偏低,且以泥沙结合态为主,轮作模式间无显著差异。田面水质劣于沟渠水,田面水对沟渠水存在不同程度增荷作用;农田水对沟渠水总氮和总磷的增荷率分别为73%和82%,其中追肥是导致农田水对沟渠水增荷的关键因素。本研究中,大蒜水稻是洱海流域农田氮、磷流失风险最高的种植模式;蚕豆水稻比大蒜水稻模式减少氮素流失风险38%。应综合考虑环境效益和经济效益,合理安排种植结构,为洱海流域种植结构调整、控制农田面源污染提供参考。  相似文献   

寓建筑美于自然美之中——浅析退思园的造园艺术特色     

唐珣  高翅 《广东园林》2011,33(1):32-35

退思园为苏州同里的一座光绪年间的私家文人园林,占地五亩,通过因地制宜的处理使其空间布局紧凑而不失疏朗.全园以水为中心,建筑全部临水而建,被著名造园学家陈从周先生誉为"贴水园".退思园全园建筑精巧,风格简朴淡雅,尤其表现在建筑与其他园林景素关系的处理上.  相似文献   

不同产地毛头鬼伞子实体多糖特征及其刺激巨噬细胞产生NO的活性   总被引：2，自引：1，他引：1  

赵源  刘艳芳  蒋俊  杨焱  唐庆九  周帅  吴迪  贾薇  冯娜  张劲松 《食用菌学报》2011,18(3):57-60

用水提醇沉法从6个不同产地毛头鬼伞(Coprinus comatus)子实体中获得6种粗多糖,6种粗多糖得率及多糖含量差异较大,以湖北(HB)的最高,其次为云南(YN)的;各粗多糖分子量分布相似,单糖组成均以葡萄糖为主,并含有少量半乳糖;除福建(FJ)外,其余5种粗多糖均能增强小鼠巨噬细胞RAW264.7释放NO的产量,以YN和HN的活性较好,总体来说,YN的多糖产量及活性较好.  相似文献