The effect of time of artificial insemination on fertilization status and embryo quality in superovulated cows   总被引：2，自引：0，他引：2  

Dalton JC  Nadir S  Bame JH  Noftsinger M  Saacke RG 《Journal of animal science》2000,78(8):2081-2085

Thirty nonlactating Holstein cows were superovulated to determine the effect of artificial insemination time on fertilization status and embryo quality. During the luteal phase of the estrous cycle, cows were administered 38 mg FSH-P in a 4-d descending dose regimen. Luteolysis was induced with two injections of prostaglandin on the last day of FSH-P treatment. All cows were continuously monitored for behavioral estrus using the HeatWatch estrus detection system. All cows were inseminated once with one .5-mL straw (50 x 10(6) sperm) at either 0 (n = 10), 12 (n = 10), or 24 h (n = 10) after the first standing event. The elapsed time (mean +/- SD) from the first prostaglandin dose to the first standing event was 39.4 h +/- 7.7 h. The (mean +/- SD) duration of behavioral estrus was 13.2 h +/- 4.1 h. The (mean +/- SD) number of standing events was 27 +/- 17. Five hundred twenty-nine embryos and ova were recovered nonsurgically 6 d after insemination. Fertilization rates were 29 (0 h), 60 (12 h), and 81% (24 h) (P < .01). Percentages of excellent and good, fair and poor, and degenerate embryos were not different (P > .05). Percentages of embryos with accessory sperm were 5 (0 h), 8 (12 h), and 41 (24 h) and differed between the 0 and 24 h and the 12 and 24 h inseminations (P < .01). Artificial insemination of superovulated, nonlactating Holstein cattle 24 h after onset of estrus increased fertilization rate and percentage of embryos with accessory sperm compared with insemination at 0 or 12 h after onset of estrus. Embryo quality was not affected by time of insemination.  相似文献   

Inclusion of dimetridazole in gamebird feeds     

Dalton JR 《The Veterinary record》2000,146(18):535

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Otto Martin Radostits 1934 – 2006     

DC Blood  CC Gay  KW Hinchcliff  PD Constable 《Australian veterinary journal》2007,85(6):231-231

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Plasma atrial natriuretic peptide concentration in warmblood horses with heart valve regurgitations     

Heidrun Gehlen PD  Dr Med Vet  Dipl ECEIM  Till Sundermann Dr Med Vet  Karl Rohn Dr Med Vet  Peter Stadler Prof  Dr Med Vet 《Journal of Veterinary Cardiology》2007,9(2):99-101

ObjectivesThis study measured plasma atrial natriuretic peptide (ANP) concentration in horses with heart valve regurgitations (HVR) with and without atrial and ventricular dilatation.BackgroundIn humans and small animals, plasma ANP concentration is increased in heart disease and correlates with the severity of clinical signs and heart enlargement.Animals, materials and methodsTen healthy horses (control) and 36 horses with HVR were evaluated by auscultation, electrocardiography, echocardiography, and determination of plasma ANP.ResultsControl horses demonstrated mean plasma ANP concentration of 21 ± 5.4 pg/mL. Of the 36 horses with HVR, 17 horses possessed normal echocardiographic heart size (group 1), 10 horses had a left atrial dilatation (group 2) and 9 horses had both left atrial and ventricular dilatation (group 3). Mean plasma ANP concentration of groups 1, 2 and 3 was 20.1 ± 5.6 pg/mL, 22.9 ± 11.0 pg/mL and 27.6 ± 17.4 pg/mL, respectively. The plasma ANP concentrations of HVR and control horses were not significantly different. The highest ANP concentrations were observed in horses with atrial and ventricular dilatation. No correlation between left atrial or ventricular size, weight, or sex and the plasma ANP concentration was found.ConclusionsNo significant differences in plasma ANP concentration was observed between groups. Further study, especially in horses with clinical signs of heart failure is needed.  相似文献   

Serological survey for antibodies against bovine viral diarrhoea virus and Neospora caninum in a population of South Australian alpacas (Vicugna pacos)          下载免费PDF全文

PD Cockcroft  LE Dornin  RC Lambert  J Perry  MP Reichel 《Australian veterinary journal》2015,93(12):476-478

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The Control of Reactive Oxygen Species Influences Porcine Oocyte In Vitro Maturation          下载免费PDF全文

GM Alvarez  SA Morado  MP Soto  GC Dalvit  PD Cetica 《Reproduction in domestic animals》2015,50(2):200-205

The aim of this study was to examine the effect of varying intracellular reactive oxygen species (ROS) levels during oocyte in vitro maturation with enzymatic ROS production systems (xanthine + xanthine oxidase or xanthine + xanthine oxidase + catalase), scavenger systems (catalase or superoxide dismutase + catalase) or cysteine on porcine oocyte maturation. Oocyte ROS levels showed an increase when H₂O₂ or O₂?^‐ production systems were added to the culture medium (p < 0.05). On the other hand, the presence of ROS scavengers in the maturation medium did not modify oocyte ROS levels compared with the control after 48 h of maturation, but the addition of cysteine induced a decrease in oocyte ROS levels (p < 0.05). The ROS production systems used in this work did not modified the percentage of oocyte nuclear maturation, but increased the decondensation of sperm head (p < 0.05) and decreased the pronuclear formation (p < 0.05). In turn, the addition of O₂?^‐ and H₂O₂ scavenging systems during in vitro maturation did not modify the percentage of oocytes reaching metaphase II nor the oocytes with decondensed sperm head or pronuclei after fertilization. However, both parameters increased in the presence of cysteine (p < 0.05). The exogenous generation of O₂?^‐ and H₂O₂ during oocyte in vitro maturation would not affect nuclear maturation or later sperm penetration, but most of the spermatozoa cannot progress to form the pronuclei after fusion with the oocyte. The decrease in endogenous ROS levels by the addition of cysteine would improve pronuclear formation after sperm penetration.  相似文献   

The Advanced Locking Plate System (ALPS): A Retrospective Evaluation in 71 Small Animal Patients     

Tomás G. Guerrero PD Dr. med vet  Diplomate ECVS  Karin Kalchofner Dr. med vet  Diplomate ECVAA  Nicole Scherrer Dr. med vet  Patrick Kircher Dr. med vet PhD  Diplomate ECVD 《Veterinary surgery : VS》2014,43(2):127-135

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Phosphofructokinase and Malate Dehydrogenase Participate in the In Vitro Maturation of Porcine Oocytes     

E Breininger  BE Vecchi Galenda  GM Alvarez  C Gutnisky  PD Cetica 《Reproduction in domestic animals》2014,49(6):1068-1073

Oocyte maturation depends on the metabolic activity of cumulus–oocyte complex (COC) that performs nutritive and regulatory functions during this process. In this work, the enzymes [phosphofructokinase (PFK) and malate dehydrogenase (MDH)] were tested to elucidate the metabolic profile of porcine COCs during the in vitro maturation (IVM). Enzymatic activity was expressed in U/COC and U/mg protein (specific activity) as mean ± SEM. In vitro maturation was performed with 2‐oxoglutarate (5, 10 and 20 mm ) or hydroxymalonate (30, 60 and 100 mm ) inhibitors of PFK and MDH, respectively. The PFK and MDH activities (U) remained constant during maturation. For PFK, the U were (2.48 ± 0.23) 10^?5 and (2.54 ± 0.32) 10^?5, and for MDH, the U were (4.72 ± 0.42) 10^?5 and (4.38 ± 0.25) 10^?5 for immature and in vitro matured COCs, respectively. The specific activities were significantly lower after IVM, for PFK (4.29 ± 0.48) 10^?3 and (0.94 ± 0.12) 10^?3, and for MDH (9.08 ± 0.93) 10^?3 and (1.89 ± 0.10) 10^?3 for immature and in vitro matured COCs, respectively. In vitro maturation percentages and enzymatic activity diminished with 20 mm 2‐oxoglutarate or 60 mm hydroxymalonate (p < 0.05). Viability was not affected by any concentration of the inhibitors evaluated. The U remained unchanged during IVM; however, the increase in the total protein content per COC provoked a decrease in the specific activity of both enzymes. Phosphofructokinase and MDH necessary for oocyte IVM would be already present in the immature oocyte. The presence of inhibitors of these enzymes impairs the meiotic maturation. Therefore, the participation of these enzymes in the energy metabolism of the porcine oocyte during IVM is confirmed in this study.  相似文献   

Effect of heating bovine colostrum at 60°C for 90′ on colostrum quality and,the health and growth characteristics of Holstein dairy calves     

A Zakian  A Rasooli  M Nouri  M Ghorbanpour  M Khosravi  PD Constable  M Moazeni 《Australian veterinary journal》2023,101(5):175-186

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IMAGING DIAGNOSIS: THE ABDOMINAL AIR-VASCULOGRAM IN A DOG WITH SPLENIC TORSION AND CLOSTRIDIAL INFECTION     

Lorrie  Gaschen  DVM  PHD  Patrick  Kircher  DVM  Claudio  Venzin  DVM  Karin  Hurter  DVM  Johann  Lang  PD  DVM 《Veterinary radiology & ultrasound》2003,44(5):553-555

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