Comparison of the complement fixation test and competitive ELISA for serodiagnosis of Anaplasma marginale infection in experimentally infected steers   总被引：1，自引：0，他引：1  

Coetzee JF  Schmidt PL  Apley MD  Reinbold JB  Kocan KM 《American journal of veterinary research》2007,68(8):872-878

OBJECTIVE: To compare sensitivity of a complement fixation (CF) test and competitive ELISA (cELISA) for detection of Anaplasma marginale in experimentally infected steers. ANIMALS: 40 crossbred (Angus-Simmental) steers. PROCEDURES: Steers were inoculated with 2.6 x 10(9) A marginale-infected erythrocytes (day 0). Blood samples were collected on days 9, 13, 20, 28, 34, 41, 61, 96, 126, and 156 days after inoculation. The percentage of parasitized erythrocytes (PPE) was determined by microscopic examination of stained blood films, and sera were evaluated with the CF test and cELISA by use of USDA-approved methods. Sensitivity and agreement (kappa statistic) between the 2 methods were determined. Persistent infections were confirmed by inoculation of blood obtained from infected steers into susceptible, splenectomized calves. RESULTS: 9 days after inoculation, sensitivity of the cELISA was 47.5%, whereas the CF test failed to identify seropositive steers. After day 13, sensitivity of the cELISA and CF test was 100% and 20%, respectively. During peak parasitemia (day 20), sensitivity of the cELISA and CF test was 100%. Thereafter, sensitivity of the CF test fluctuated between 7.5% and 37.5%, whereas sensitivity of the cELISA remained at 100%. Overall sensitivity of the cELISA and CF test was 94.8% and 26.5%, respectively (kappa statistic, 0.039). CONCLUSIONS AND CLINICAL RELEVANCE: The cELISA had superior sensitivity for serologic detection of A marginale.The CF test and cELISA each had a high percentage of false-negative results during the prepatent period. These findings are relevant for export certification and anaplasmosis prevention or eradication programs.  相似文献   

Facet joint geometry and intervertebral disk degeneration in the L5-S1 region of the vertebral column in German Shepherd dogs     

Seiler GS  Häni H  Busato AR  Lang J 《American journal of veterinary research》2002,63(1):86-90

OBJECTIVE: To evaluate the possible association between facet joint geometry and intervertebral disk degeneration in German Shepherd Dogs. ANIMALS: 25 German Shepherd Dogs and 11 control dogs of similar body weight and condition. PROCEDURE: Facet joint angles in the caudal portion of the lumbar region of the vertebral column (L5-S1) were measured by use of computed tomography, and the intervertebral discs were evaluated microscopically. The relationship between facet joint geometry and disk degeneration was evaluated by use of statistical methods. RESULTS: German Shepherd Dogs had significantly more facet joint tropism than control dogs, but an association with disk degeneration was not found. However, German Shepherd Dogs had a different facet joint conformation, with more sagittally oriented facet joints at L5-L6 and L6-L7 and a larger angle difference between the lumbar and lumbosacral facet joints, compared with control dogs. CONCLUSIONS AND CLINICAL RELEVANCE: A large difference between facet joint angles at L6-L7 and L7-S1 in German Shepherd Dogs may be associated with the frequent occurrence of lumbosacral disk degeneration in this breed.  相似文献   

Vermicompost as a substitute for peat in potting media: Effects on germination,biomass allocation,yields and fruit quality of three tomato varieties   总被引：1，自引：0，他引：1  

Johann G. Zaller 《Scientia Horticulturae》2007

Commercial potting media often contain substantial amounts of peat that was mined from endangered bog and fen ecosystems. The main objectives of this study were to assess (1) whether the amendment of 0, 20, 40, 60, 80 and 100% (v/v) of vermicompost (VC) to a fertilized commercial peat potting substrate has effects on the emergence, growth and biomass allocation of tomato seedlings (Lycopersicon esculentum Mill.) under greenhouse conditions, (2) whether possible impacts on seedlings can affect tomato yields and fruit quality even when transplanted into equally fertilized field soil, and (3) whether effects are consistent among different tomato varieties. Amended VC was produced in a windrow system of food and cotton waste mainly by earthworms Eisenia fetida Sav. Vermicompost amendments significantly influenced, specifically for each tomato variety, emergence and elongation of seedlings. Biomass allocation (root:shoot ratio) was affected by VC amendments for two varieties in seedling stage and one field-grown tomato variety. Marketable and total yields of field tomatoes were not affected by VC amendments used for seedling husbandry. However, morphological (circumference, dry matter content, peel firmness) and chemical fruit parameters (contents of C, N, P, K, Ca, Mg, l-ascorbic acid, glucose, fructose) were significantly affected by VC amendments in seedling substrates; these effects again were specific for each tomato variety. Overall, vermicompost could be an environmentally friendly substitute for peat in potting media with similar or beneficial effects on seedling performance and fruit quality. However, at least for tomatoes, variety-specific responses should be considered when giving recommendations on the optimum proportion of vermicompost amendment to horticultural potting substrate.  相似文献   

ULTRASONOGRAPHIC DETECTION OF ADRENAL GLAND TUMOR AND URETEROLITHIASIS IN A GUINEA PIG   总被引：1，自引：0，他引：1  

Lorrie  Gaschen  DVM  Cornelia  Ketz  Dr. med. vet.  Johann  Lang  Dr. med. vet  Urs  Weber  Dr. med. vet.  L. Bacciarini  Dr. med. vet.  Ingrid  Kohler  med. vet. 《Veterinary radiology & ultrasound》1998,39(1):43-46

A 5-year-old guinea pig was presented to the University of Berne Small Animal Radiology Department for an ultrasound examination of the abdomen to confirm a suspected diagnosis of Cushing's syndrome. The patient had bilateral alopecia, was apathic and obese. Ultrasonographically, a tumor of the left adrenal gland, obstruction of the left ureter by an ureterolith, as well as hydronephrosis of the left kidney were detected. During surgery to relieve the ureteral obstruction the adrenal gland tumor was removed. The guinea pig died post-operatively due to blood loss. The left adrenal gland tumor was found histopathologically to be an adenoma and the right adrenal gland also had multiple small adenomas, but grossly appeared normal. The ureterolith was analyzed and found by x-ray diffraction to consist of calcium carbonate.  相似文献   

Die Königlich Bayerische forstverwaltung und ihre geschichtliche Entwicklung im 19. Jahrhundert     

Johann Keiper 《European Journal of Forest Research》1907,29(11):575-598

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Estimation of genetic and crossbreeding parameters for daily milk yield of Ayrshire × Sahiwal × Ankole crossbred cows in Burundi     

Gilbert Hatungumukama  Johann Detilleux 《Livestock Science》2009,122(2-3):359-362

The pedigree of 317 cows of which 184 were controlled for milk production has been used to estimate crossbreeding parameters for daily milk yield of Ayrshire, Sahiwal and Ankole crosses in the Mahwa station. Lactating cows belonged to one of 6 different genetic groups defined on the basis of the mating system used to produce them. REML estimates of the genetic parameters were obtained with a repeated animal model using daily milk records. Estimated heritability (h²) and repeatability (r²) were 0.27 and 0.36, respectively. The genetic group effects were used to estimate crossbreeding parameters following Dickerson's genetic model. Estimates for the additive effects for daily milk yield of Ankole, Sahiwal and Ayrshire breeds were − 1.66l, − 0.48l and 5.22l, respectively. Estimates of direct heterosis for daily milk yield for Sahiwal × Ankole, Ayrshire × Ankole, and Ayrshire × Sahiwal crosses were 1.97l, 2.30l and − 2.33l, respectively.  相似文献   

Höhenmessung mittels Blume-Leiß-Höhenmesser bei variabler Entfernung zum Meßbaum     

K. Johann 《European Journal of Forest Research》1974,93(1):86-91

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The role of fire in forest dynamics in Daxinganling region     

Johann G. Goldammer  Di Xucying 《林业研究》1990,1(1):36-41

Recent fire statistics and preliminary fire history data suggest that fire has been historically responsible for maintaining the vegetative communities up to present in Daxinganling region. Forest types, and even tree species, arc dependent on the degree of fire intensity, fire size, depth of burn and fire frequency. Selected samples of larch, pine, birch and spruce forest were studied in terms of species composition as determined by fire frequency which mainly depends on topography and site conditions. Intervals between fires range between 6 and 170 years.  相似文献   

Describing the sticking phenomenon of aminoplastic resins: introduction of a new test method     

Alexandra Himsel  Johann Moser  Wolfgang Kantner  Roland Mitter  Jürgen Gießwein  Hendrikus W. G. van Herwijnen  Ulrich Müller 《Wood Science and Technology》2015,49(4):681-694

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DISPERSAL PATTERN OF INJECTATE FOLLOWING CT‐GUIDED PERINEURAL INFILTRATION IN THE CANINE THORACOLUMBAR SPINE: A CADAVER STUDY          下载免费PDF全文

Sibylle Kneissl  Sabine Breit  Florian Willmitzer  Johann Thalhammer  Sabine Dengg 《Veterinary radiology & ultrasound》2015,56(2):212-219

An increasing proportion of canine patients are presented with chronic thoracolumbar back pain and without compressive spinal lesions. In humans, spinal perineural infiltrations have been reported to have a favorable effect on pain control. The purpose of this prospective cadaver study was to describe the dispersal pattern of injectate following CT‐guided spinal perineural infiltration in the canine thoracolumbar region. Seven fresh canine cadavers were first scanned using multislice CT and then CT‐guided spinal perineural infiltration was performed at 42 sites from T13/L1 to L6/L7. The injectate for each site was a mixture of new methylene blue and iodinated contrast medium. Immediately following CT‐guided injection, cadavers were frozen, cut, and dissected macro‐ and mesoscopically (using a magnifying glass) to identify anatomic structures that were infiltrated. In the majority of sites (64.3%), complete epidural and hypaxial staining of spinal nerve components (including the spinal ganglion, trunk, and ventral branch) was successfully achieved. However, no (11.9%) or unpredictable staining (9.5%) of nervous tissue occurred in some sites despite careful CT guidance and the application of relatively large volumes of injectate. Optimal results were achieved when the needle tip was positioned periforaminally ventral to the cranial contour of the cranial articular process. Findings from this ex vivo study indicated that CT‐guided spinal perineural infiltration is feasible for testing in the canine thoracolumbar region and that successful nerve tissue infiltration would likely occur in the majority of sites. Future in vivo studies are needed to determine the safety and efficacy of this technique.  相似文献