首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   171篇
  免费   2篇
林业   10篇
农学   17篇
基础科学   1篇
  39篇
农作物   55篇
水产渔业   14篇
畜牧兽医   21篇
园艺   3篇
植物保护   13篇
  2023年   2篇
  2022年   6篇
  2021年   14篇
  2020年   14篇
  2019年   14篇
  2018年   10篇
  2017年   14篇
  2016年   12篇
  2015年   10篇
  2014年   12篇
  2013年   17篇
  2012年   9篇
  2011年   11篇
  2010年   8篇
  2009年   5篇
  2008年   8篇
  2007年   2篇
  2006年   1篇
  2005年   1篇
  2004年   1篇
  2003年   2篇
排序方式: 共有173条查询结果,搜索用时 15 毫秒
61.
Net primary production (NPP) is an indicator of rangeland ecosystem function. This research assessed the potential of the Carnegie Ames Stanford Approach (CASA) model for estimating NPP and its spatial and temporal changes in semi-arid rangelands of Semirom County, Iran. Using CASA model, we estimated the NPP values based on monthly climate data and the normalized difference vegetation index (NDVI) obtained from the MODIS sensor. Regression analysis was then applied to compare the estimated production data with observed production data. The spatial and temporal changes in NPP and light utilization efficiency (LUE) were investigated in different rangeland vegetation types. The standardized precipitation index (SPI) was also calculated at different time scales and the correlation of SPI with NPP changes was determined. The results indicated that the estimated NPP values varied from 0.00 to 74.48 g C/(m2?a). The observed and estimated NPP values had different correlations, depending on rangeland conditions and vegetation types. The highest and lowest correlations were respectively observed in Astragalus spp.-Agropyronspp. rangeland (R2=0.75) with good condition and Gundeliaspp.-Cousiniaspp. rangeland (R2=0.36) with poor and very poor conditions. The maximum and minimum LUE values were found in Astragalus spp.-Agropyronspp. rangeland (0.117 g C/MJ) with good condition and annual grasses-annual forbs rangeland (0.010 g C/MJ), respectively. According to the correlations between SPI and NPP changes, the effects of drought periods on NPP depended on vegetation types and rangeland conditions. Annual plants had the highest drought sensitivity while shrubs exhibited the lowest drought sensitivity. The positive effects of wet periods on NPP were less evident in degraded areas where the destructive effects of drought were more prominent. Therefore, determining vegetation types and rangeland conditions is essential in NPP estimation. The findings of this study confirmed the potential of the CASA for estimating rangeland production. Therefore, the model output maps can be used to evaluate, monitor and optimize rangeland management in semi-arid rangelands of Iran where MODIS NPP products are not available.  相似文献   
62.
An in vitro regeneration system was developed using organogenic callus derived from in vitro grown cotyledonary explants of Gleditsia caspica Desf., an important leguminous tree. Murashige and Skoog (MS) basal medium augmented with 0.2 g L?1 myo-inositol and various concentrations of either 2,4-dichlorophenoxyacetic acid (2,4-D), naphthaleneacetic acid, or indole-3-butyric acid (IBA) alone as well as combined with cytokinins was used for callus induction. The highest frequency of organogenic yellowish-white and nodular callus (93 %) was obtained from explants grown on medium supplemented with 13.5 μM 2,4-D and 4.4 μM benzyladenine (BA). The yellowish-white and nodular callus when transferred to MS medium supplemented with BA (2.2–17.7 μM) or kinetin (KT; 2.3–18.8 μM) solely or in combination with 2.3 μM 2,4-D produced several microshoots after 5 weeks culture. The calli cultured on MS medium with 4.4 μM BA singly showed superior growth response and produced both maximum shoot regeneration (94 %) and the highest mean number (4.3) of microshoots per callus. Transfer of regenerated microshoots onto modified MS basal medium fortified with 5.8 μM gibberellic acid and 4.4 μM BA resulted in the maximum number of internodes per shoot and the highest shoot elongation after a period of 6 weeks. Optimum rooting of 90 %, an average 6.1 roots per shoot, and a mean root length of 3.6 cm was observed when half-strength MS medium was supplemented with 9.8 μM IBA and 0.92 μM KT. The regenerated healthy plants with well-developed shoots and roots showed a survival rate of 77 % after acclimatization and transplanting to garden soil for a 10-week hardening period under ex vitro conditions.  相似文献   
63.
The influence of dietary fish oil (FO) substitution with blends of vegetal oils (VO: canola, linseed, olive, sunflower, corn and coconut oils) in plant protein‐rich diets on reproductive performance and fatty acid dynamics of embryos was evaluated in female rainbow trout (Oncorhynchus mykiss) brooders (1.8 ± 0.1 kg). Four diets were formulated in which 20% (FO80/VO20), 50% (FO50/VO50), 75% (FO25/VO75) and 100% (VO100) of FO were replaced by mixture of VO. The above‐mentioned diets were administered for a short period prior to spawning (3 months). Fish fed the VO100 had the lowest fertilization rate (81.3 ± 2.3), whereas brooders fed the FO80/VO20 diet had the lowest survival rates at eyed embryo stage (83.7 ± 1.6%) and hatching rate (79.9 ± 3.1%). The fatty acid dynamics of embryos were not only affected by embryonic developmental stages, but also they were influenced by the dietary fatty acid profile. Our study confirmed that using blends of different VO sources and FM residual fat in the FO25/VO75 for the short‐term period prior to spawning seemed to be a good strategy in terms of successful reproduction for sparing high levels of FM and FO in diet of O. mykiss brooders.  相似文献   
64.
A 90‐day feeding trial was carried out to examine the influence of fish oil (FO) substitution with blends of vegetable oils (VOs) on reproductive efficiency of female brooders and fluctuation in fatty acid (FA) profile of embryos in Oncorhynchus mykiss. A basal diet was formulated in which 20% (80FO/20VO), 50% (50FO/50VO), 75% (25FO/75VO) and 100% (100VO) of FO were replaced by mixture of VO. Reproductive performance of brooders was not affected by drastic alternations in FA profile of diets. The level of saturated and monounsaturated FAs (MUFAs) significantly increased, whereas the levels of long‐chain polyunsaturated FAs (LC‐PUFAs), mainly docosahexaenoic acid, profoundly decreased during embryogenesis. The concentrations of MUFA and n?6 PUFA increased in the eggs with increasing the incorporation of VO mixture in diets; however, the concentration of LC‐PUFA and n?3/n?6 PUFA ratio decreased. Haematological parameters and humoral immune responses including total immunoglobulin content, lysozyme and alternative complement pathway activities in brooders fed with the experimental diets did not statically different. In summary, incorporating mixture of various VO sources especially linseed and sunflower oils as good sources of α‐linolenic and linoleic acids, respectively, along with low levels of residual fat from fish meal in diet suggested a good strategy for providing the appropriate essential FA requirements of O. mykiss brooders for their successful reproduction.  相似文献   
65.
Pacific white shrimp (Litopenaeus vannamei) is an important species in aquaculture worldwide. Post-mortem changes during cold storage reduce the quality of shrimp and shorten its shelf life. Cold plasma (CP) was applied for 45, 90, and 150s as T1, T2, and T3, respectively, to fresh harvested shrimp. The biochemical parameters and melanosis were analyzed in comparison to metabisulphite treated (TM) and control during 12 days of refrigerated storage. The results highlighted lower rate of increase in pH, total volatile base nitrogen (TVB-N) content, thiobarbituric acid reactive substances (TBARS), free fatty acid (FFA), peroxide value (PV), and fluorescent compounds (FC) in shrimp exposed to CP and metabisulphite compared to control (P < 0.05). Although CP lowered biochemical changes in T1 and T3 shrimp, T2 was the most effective treatment in reducing undesirable changes and improving quality of shrimp. In all storage periods, melanosis was significantly lower in T2, T3, and TM samples. Results demonstrate that CP exposure effectively decreases relative activity of polyphenol oxidase (PPO) enzyme, and prolonging the exposure to CP for 150 s resulted in 50% reduction in enzyme activity. We conclude that application of CP for 90 s was the most efficient circumstance to extend shelf life of white shrimp during cold storage.  相似文献   
66.
The presence and vasoactive effects of native calcitonin gene-related peptide (CGRP), substance P (SP), and neurokinin A (NKA) were studied on isolated small branches of the coeliac artery from Atlantic cod, Gadus morhua, using immunohistochemistry and myograph recordings, respectively. Immunohistochemistry revealed nerve fibers containing CGRP- and SP/NKA-like material running along the wall of the arteries. CGRP induced vasorelaxation of precontracted arteries with a pD2 value of 8.54 ± 0.17. Relaxation to CGRP (10−8 M) was unaffected by l-NAME (3 × 10−4 M) and indomethacin (10−6 M) suggesting no involvement of nitric oxide or prostaglandins in the CGRP-induced relaxation. SP and NKA (from 10−10 to 3 × 10−7 M) contracted the unstimulated arteries at concentrations from 10−8 M and above in 42% and 33%, respectively, of the vessels. It is concluded that the innervation of the cod celiac artery includes nerves expressing CGRP-like and tachykinin-like material, and that a vasodilatory response to CGRP is highly conserved amongst vertebrates while the response to tachykinins is more variable.  相似文献   
67.

Over the last two decades, the highly pathogenic avian influenza H5N1 virus has gained a lot of attention due to its zoonotic and mutative nature. Iran is among the countries significantly affected by the virus as it hosts migratory birds during seasonal migration. In this study, the molecular characterizations of hemagglutinin (HA) and neuraminidase (NA) genes and proteins of H5N1 strain A/chicken/Iran/8/2015 detected in backyard poultry, Mazandaran province, were investigated. Phylogenetic analysis classified this virus as a member of subclade 2.3.2.1c, with the cleavage site motif of “PQRERRRK-R/GLF”. HA carried a few mutations altering affinity to mammalian cells; however, the virus was categorized as avian. NA protein had the 20-amino acid deletion at aa position 49–69 similar to those isolated since 2000. Mutations of H253Y and H274Y contributing to antiviral resistance were present in NA. From this analysis, it can be concluded that the wild migratory birds flying from Western Asia to Eastern Africa are probably the main carriers of seasonal H5N1 in the country.

  相似文献   
68.
The main objective of this study was to evaluate the potential protective effect of red Grape Seed Extract (GSE) on lead induced hypertension (HTN) and Heart Rate (HR) in male Wistar rats. The rats were randomly assigned to one of 4 groups: Each group received lead acetate (100 ppm in drinking water), GSE (100 mg kg(-1), orally) or Lead + GSE for 45 days. Another group assigned as control group provided with tap water and regular pellet food. The Systolic Blood Pressure (SBP) and heart rate were determined by tail plethysmography coupled to a computer system. There was a sustained elevation of SBP in lead exposed rats that significantly increased at day 18 (lead treated, 112.7 +/- 2.7 mmHg, vs. control, 105.6 +/- 2.6 mmHg, n = 10, p < 0.05) and reached a maximum level at day 36 (lead treated, 124.9 +/- 2.3 mmHg, vs. control, 103.6 +/- 3.1 mmHg, n = 10, p < 0.001). However, the other three groups; showed no significant changes in SBP. Furthermore, the heart rate was increased sustainly in lead exposed animals that was statistically significant at days 36 and 45 (lead treated group, 404.5 +/- 9.4 vs. control group, 381.7 +/- 6.7, n = 10, p < 0.05). The blood lead level in both lead and lead + GSE treated groups was increased significantly compared with control and GSE treated groups (p < 0.001). However, GSE administration had no effect on the blood lead level in lead treated group. According to the result of this study, it may be concluded that GSE could have beneficial effect in protecting the cardiovascular system through its antioxidant activity against oxidative stress.  相似文献   
69.
In two identical pot trials, we investigated the effect of different biochars on the growth and water use efficiency (WUE) of fenugreek (Trigonella foenum-graecum L.) grown with and without water stress. Plants were grown in pots with and without biochar. Rice husk (RH), standard rice husk (SRH), standard wheat straw (SWS) or standard oilseed rape straw (SOS) biochars were added at a rate of 2% by weight. Irrigation was reduced from 80% of field capacity to 40% in half of the pots to create water stress when 50% of the plants flowered. No significant interaction was observed between irrigation and biochar treatments. Water stress resulted in smaller plants (11.5%), fewer pods (16.1%) and reduced 100-seed weight (12.6%), seed yield (20.1%) and plant dry weight (55.0%). All standard biochars increased plant dry weight in a range of 18%−25% compared with the controls. There was no significant effect of biochar on the number of pods and seed weight. Only RH, SWS and SOS biochar increased the number of seeds pod−1 by 9.0%−14.5% compared with the controls regardless of water stress. RH and SOS biochars increased seed yield by 16.7 and 21.6%, respectively. RH, SWS and SOS biochar increased the WUE by 17.3%, 10.1% and 16.2% regardless of water stress, respectively. Based on the experiment, we recommend farmers to add biochar whatever type they have available. However, RH and SOS seem to be better than the others are, as they seem to be able to increase seed yield and WUE considerably.  相似文献   
70.
Viruses are obligatory intracellular parasites that use cell proteins to take the control of the cell functions in order to accomplish their life cycle. Studying the viral-host interactions would increase our knowledge of the viral biology and mechanisms of pathogenesis. Studies on pathogenesis mechanisms of lyssaviruses, which are the causative agents of rabies, have revealed some important host protein partners for viral proteins, especially for most studied species, i.e. RABV. In this review article, the key physical lyssavirus-host protein interactions, their contributions to rabies infection, and their exploitation are discussed to improve the knowledge about rabies pathogenesis. Key Words: Host-Pathogen Interactions, Lyssavirus, Rabies  相似文献   
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号