夏秋蚕新品种浒花、秋星的育成及其一代杂交种的选配     

何毅  司马杨虎 《蚕业科学》1991,17(4):200-207

根据长江流域夏秋季的气候特点,采用杂交、回交、系统分离、活蛹缫丝和多代高温多湿环境定向培育等育种方法,分别育成了中系蚕品种“浒花”和日系蚕品种“秋星”。两者都具有抗性强、丝量高、茧丝质优以及繁育容易等特点。其一代杂交种经多次实验室鉴定、农村生产鉴定、试养以及全国蚕品种审定结果表明:该新品种体质强健、孵化、眠起、老熟齐一,茧丝量高,茧层率22％;鲜毛茧出丝率15—16％,茧丝品质优良、解舒率74％,净度93分,茧丝纤度2.53/D,茧丝长超1000m。  相似文献   

家蚕EST数据库的应用现状及展望     

蔡明文房丽秀  司马杨虎 《江苏蚕业》2007,29(3):9-12

家蚕是重要的经济昆虫,也是鳞翅目昆虫的主要模式生物。表达序列标签(EST)在家蚕研究中的应用越来越受到关注。本文主要介绍了EST数据库的建立及其在家蚕研究中的应用现状,展望了EST在家蚕中的研究趋势。  相似文献   

盐酸处理蚕卵酯酶A4的活性变化及其与滞育性的关系   总被引：10，自引：6，他引：4  

徐世清  甲斐英则 《蚕业科学》1998,24(2):95-99

从家蚕C108品种蚕卵精制酯酶A4,首次测定了产卵后24～96h浸酸蚕卵酯酶A4的ATPase活性。蚕卵经盐酸处理后在体外5℃1～2d或体外25℃1～3h出现酶活性峰。与低温活化蚕卵比较,产卵后24～96h的浸酸蚕卵,在体外5℃酶活性峰出现时间提早了8．5～22d,在体外25℃提早了3～9h。浸酸后蚕卵迅速解除滞育,滞育发育时间显著缩短。将不同卵龄未浸酸蚕卵与盐酸处理蚕卵酯酶A4的ATPase活性峰出现时间差制成曲线,在体外5℃或25℃的结果几乎都完全与5℃冷藏时蚕卵的滞育发育时间曲线重叠。盐酸处理蚕卵酯酶A4的活性变化与蚕卵活化有高度相关性。  相似文献   

Clinicopathological studies on facial eczema outbreak in sheep in Southwest Turkey     

Ozmen O  Sahinduran S  Haligur M  Albay MK 《Tropical animal health and production》2008,40(7):545-551

After very hot summer, 22 sheep from 5 different flocks consisting of approximately 150-200 animals each were diagnosed with facial eczema in September 2005, in southwest Turkey. Photophobia, corneal opacity, severe ulcers of the facial skin, especially localized around the eyes and mouth, and 3% mortality were the most prominent clinical symptoms. GGT levels of the animals were very high and varying between 261- 328 U/l. While the activities of ALT and total bilirubin were elevated and AST was normal in affected sheep. Total bilirubin level was higher than normal. Seven of the 22 sheep were euthanatized and necropsy was performed on all of these animals. Severe icterus, hepatomegaly, enlarged gallbladder, congestion of mesenteric vessels were the common necropsy findings. Histopathological changes of the liver included necrosis of the hepatocytes, cholangiohepatitis characterized by mononuclear inflammatory cell infiltrate in the portal area and mild to severe fibrosis around bile ducts. A diagnosis of sporidesmin toxicosis was made based on the histopathology of the livers, the elevation in liver enzymes, and the development of cutaneous lesions consistent with photosensitization and high spore counts in the ruminal contents. Surviving sheep were treated with procaine penicillin + dihidrostreptomycin sulfate, multivitamin complexes and flunixin meglumine. Additionally, zinc sulphate was also given at a dose of 6 gr per 100 lt drinking water for 28 days. All treated sheep recovered. Pasture spore counts were between 96,300- 267,500 spores/g grass.  相似文献   

Impact of silicon-based quantum dots on the antioxidative system in white muscle of Carassius auratus gibelio     

Stanca L  Petrache SN  Radu M  Serban AI  Munteanu MC  Teodorescu D  Staicu AC  Sima C  Costache M  Grigoriu C  Zarnescu O  Dinischiotu A 《Fish physiology and biochemistry》2012,38(4):963-975

Silicon-based quantum dots were intraperitoneally injected in individuals of Carassius auratus gibelio. Their effects on white muscle were investigated by following their distribution and impact on the antioxidative system. The GSH level significantly increased after 1 and 3?days of exposure by, respectively, 85.3 and 25.4%. Seven days later, GSH levels were similar to control concentrations. MDA concentration rose after three days by 46.9% and remained at the same level after 7?days. Protein thiol levels significantly decreased by 6.7 and 8.1% after 3 and 7?days, whereas advanced oxidation protein products increased by 12.7, respectively, 28.1% in the same time intervals. The protein reactive carbonyl groups were raised only after the first day of exposure and returned to the control level later on. SOD specific activity increased up to 48% after 7?days, while CAT activity increased by 328, 176, and 26% after 1, 3, and 7?days of treatment. GST specific activity was up-regulated by 87, 18, and 9%, while GR activity increased by 68, 34, and 9%. G6PD activity was up-regulated by 12, 22, and 50%, whereas GPx activity raised by 75 and 109% compared to control after, respectively, 1, 3, and 7?days. Our results suggest that oxidative stress induced by silicon-based quantum dots was not strong enough to cause permanent damage in the white muscle of crucian carp.  相似文献   

An in silico approach for characterization of encoded protein from Rdr1, a black spot resistance gene in Rosa multiflora     

Taheri  Sima  Abdullah  Thohirah Lee  Sahebi  Mahbod  Azizi  Parisa 《植物病害和植物保护杂志》2017,124(4):319-330

Journal of Plant Diseases and Protection - Black spot disease is the most common diseases of landscape roses and is caused by Diplocarpon rosae Wolf. In Rosa multiflora, the screening of black...  相似文献   

Leishmania Parasite: the Impact of New Serum-Free Medium as an Alternative for Fetal Bovine Serum     

Sima Habibzadeh  Delaram Doroud  Tahereh Taheri  Negar Seyed  Sima Rafati 《Iranian Biomedical Journal》2021,25(5):349

Background:Flagellated protozoan of the genus Leishmania is the causative agent of vector-borne parasitic diseases of leishmaniasis. Since the production of recombinant pharmaceutical proteins requires the cultivation of host cells in a serum-free medium, the elimination of FBS can improve the possibility of large-scale culture of Leishmania parasite. In the current study, we aimed at evaluating a new serum-free medium in Leishmania parasite culture for future live Leishmania vaccine purposes. Methods:Recombinant L. tarentolae secreting PpSP15-EGFP and wild type L. major were cultured in serum-free (CSFM) and serum-supplemented medium. The growth rate, protein expression, and infectivity of cultured parasites in both conditions was then evaluated and compared. Results:Diff-Quick staining and epi-fluorescence microscopy examination displayed the typical morphology of L. major and L. tarentolae-PpSP15-EGFP promastigote grown in CSFM medium. The amount of EGFP expression was similar in CSMF medium compared to M199 supplemented with 5% FBS in flow cytometry analysis of L. tarentolae-PpSP15-EGFP parasite. Also, a similar profile of PpSP15-EGFP proteins was recognized in Western blot analysis of L. tarentolae-PpSP15-EGFP cultured in CSMF and the serum-supplemented medium. Footpad swelling and parasite load measurements showed the ability of CSFM medium to support the L. major infectivity in BALB/C mice. Conclusion:This study demonstrated that CSFM can be a promising substitute for FBS supplemented medium in parasite culture for live vaccination purposes. Key Words: Growth rate, L. major, L. tarentolae, Serum-free medium, PpSP15-EGFP protein  相似文献   

Humoral and cellular immune responses against Type I cysteine proteinase of Leishmania infantum are higher in asymptomatic than symptomatic dogs selected from a naturally infected population     

Nakhaee A  Taheri T  Taghikhani M  Mohebali M  Salmanian AH  Fasel N  Rafati S 《Veterinary parasitology》2004,119(2-3):107-123

Canids are natural reservoirs of Leishmania infantum and have been promoted as experimental hosts to decipher the pathogenesis of human visceral leishmaniasis (VL). In this study, the presence of IgG antibodies as well as the presence of mononuclear leukocytes reactive to different cysteine proteinases (CPs) were examined in 13 L. infantum-infected dogs (six with symptoms, seven asymptomatic). Cysteine proteinases which belong to papain-like enzymes known as clan CA are the most studied CPs of parasite protozoa. These molecules are expressed by the intracellular stages of the parasite and could be immunogenic. We studied Type II CP (CPA) and Type I CP (CPB) with its long C-terminal extension (CTE) which could be highly immunogenic. We showed that the level of antibodies reactive to rCPA is low in both symptomatic and asymptomatic dogs. In contrast, when CPB and CTE were used as antigens, the level of total IgG (with IgG2 superior to IgG1) reached higher values in asymptomatic dogs than in dogs with VL. While the peripheral blood mononuclear cell (PBMC) reactivity was significant when cultured in the presence of freezed/thawed (F/T) lysate, it remained low in presence of CP although always higher for PBMC recovered from asymptomatic dogs. We showed the importance of CPB and CTE in particular as a target of immune response and their potential use for serodiagnosis in asymptomatic dogs.  相似文献   

A survey on hydatidosis in livestock in Northern Iran based on data collected from slaughterhouses from 2004 to 2008     

Mansoorlakooraj H  Saadati D  Javadi R  Heydari S  Torki E  Gholami H  Fard RM 《Veterinary parasitology》2011,182(2-4):364-367

Data have been collected from slaughterhouses in three provinces across the Northern Iran (Gilan, Mazandaran and Golestan) from March 2004 to March 2008. These data were collected to evaluate the prevalence of hydatidosis in slaughtered cattle, sheep and goats. During the study, 3,347,797 animals were slaughtered. These included 621,686 cattle, 1,719,725 sheep and 1,006,386 goats. The prevalence of infection in cattle, sheep and goats was 12%, 14.6% and 10.1%, respectively. The association of condemnation rates with seasons was not proven statistically.  相似文献   

温度对AFLP银染检测的影响   总被引：11，自引：0，他引：11  

陈大霞  司马杨虎  赵爱春  鲁成 《蚕学通讯》2003,23(1):1-4

在家蚕AFLP分子标记多样性的研究中发现温度对AFLP—银染的结果有一定的影响。本实验以湘晖与872杂交的91个F2个体为材料，对AFLP银染过程中温度的影响进行了分析。温度试验结果显示：在25℃左右进行胶板的前期处理和凝胶的制备为最佳温度。  相似文献