Mortality outbreak by perch rhabdovirus in European perch (Perca fluviatilis) farmed in Italy: Clinical presentation and phylogenetic analysis     

Claudio Caruso  Andrea Gustinelli  Paolo Pastorino  Pier Luigi Acutis  Riccardo Prato  Loretta Masoero  Simone Peletto  Maria Letizia Fioravanti  Marino Prearo 《Journal of fish diseases》2019,42(5):773-776

This work reports a mortality outbreak, occurred in 2015 and affecting juveniles of European perch (Perca fluviatilis L.) farmed in Italy. Perch rhabdovirus (PRV) was detected by viral isolation and biomolecular investigations. Phylogenetic analysis clustered our isolate into genogroup B, which also includes PRV isolates from Perca fluviatilis identified in France (2004–2009); diagnostic investigations also revealed opportunistic bacteria (Aeromonas hydrophila) and parasites (Chilodonella piscicola). Since, occasionally, PRV has been reported in the natural environment, which is often a source of eggs and broodstock for farms, it could be possible that both similar France and Italian isolate were imported from a same place elsewhere and have a common origin. Improving biosecurity measures (batch control) and disinfection of egg strings with an iodine‐based solution helps prevent apparent vertical transmission of PRV.  相似文献   

STAR: An integrated and standardized procedure to evaluate the ecological status of coralligenous reefs     

Luigi Piazzi  Paola Gennaro  Monica Montefalcone  Carlo Nike Bianchi  Enrico Cecchi  Carla Morri  Fabrizio Serena 《水产资源保护:海洋与淡水生态系统》2019,29(2):189-201

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Assessment of the Antimicrobial Activity of Algae Extracts on Bacteria Responsible of External Otitis     

Gianluca Pane  Gabriele Cacciola  Elisabetta Giacco  Gian Luigi Mariottini  Erika Coppo 《Marine drugs》2015,13(10):6440-6452

External otitis is a diffuse inflammation around the external auditory canal and auricle, which is often occurred by microbial infection. This disease is generally treated using antibiotics, but the frequent occurrence of antibiotic resistance requires the development of new antibiotic agents. In this context, unexplored bioactive natural candidates could be a chance for the production of targeted drugs provided with antimicrobial activity. In this paper, microbial pathogens were isolated from patients with external otitis using ear swabs for over one year, and the antimicrobial activity of the two methanol extracts from selected marine (Dunaliella salina) and freshwater (Pseudokirchneriella subcapitata) microalgae was tested on the isolated pathogens. Totally, 114 bacterial and 11 fungal strains were isolated, of which Staphylococcus spp. (28.8%) and Pseudomonas aeruginosa (P. aeruginosa) (24.8%) were the major pathogens. Only three Staphylococcus aureus (S. aureus) strains and 11 coagulase-negative Staphylococci showed resistance to methicillin. The two algal extracts showed interesting antimicrobial properties, which mostly inhibited the growth of isolated S. aureus, P. aeruginosa, Escherichia coli, and Klebsiella spp. with MICs range of 1.4 × 10⁹ to 2.2 × 10¹⁰ cells/mL. These results suggest that the two algae have potential as resources for the development of antimicrobial agents.  相似文献   

Residues of a Triazole Fungicide in Soil After 4 Years of Application to Sugar Beet     

Luigi Lucini  Paloma Magistrati  Gian Pietro Molinari 《Water, air, and soil pollution》2009,202(1-4):13-18

An experimental study to investigate the potential soil accumulation of the triazole fungicide difenoconazole in soil was carried out in northwestern Italy. The fungicide was applied to sugar beet for 4 years with three applications per year at a rate of 75 g ha^?1 each, according to formulated product recommended use. Soil cores were collected each year before the first application, after each application and at harvest of the crop. The soil samples were then split into 0–10 and 10–40 cm depth layers, extracted and quantitatively analysed by gas chromatography for difenoconazole residues. The study evidenced that difenoconazole residues could be detected in the upper soil layer only, in quantities detectable after several applications (0.14 to 0.32 mg kg^?1 after the third application) which then become undetectable the following year. It can be concluded, therefore, that difenoconazole does not accumulate in soil.  相似文献   

Soil chemical and biochemical properties of a salt-marsh alluvial Spanish area after long-term reclamation   总被引：2，自引：0，他引：2  

Vito Armando Laudicina  Maria Dolores Hurtado  Luigi Badalucco  Antonio Delgado  Eristanna Palazzolo  Michele Panno 《Biology and Fertility of Soils》2009,45(7):691-700

Marisma, one of the largest salt-marsh alluvial areas in SW Spain, has been reclaimed since 1970 by artificial drainage and amendment with phosphogypsum (PG) so as to reduce Na⁺ saturation. Within the reclaimed area, two 250- × 20-m plots were treated as follows: (1) amendment with 25 Mg/ha of PG every 2 to 3 years between 1979 and 2003 (plot PY); (2) like PY but PG treatment stopped after 1997 (plot DR). A contiguous virgin Marisma salt-marsh plot (MV), neither drained nor amended, was the control. In MV, soil microbial biomass C, most enzyme activities and total organic C content were much greater than in PY and DR soils, despite the salinity stress. The decrease in soil organic matter content in PY and DR soils was likely due to cotton-cropping practices, which favoured the organic C mineralisation and nutrients removal by crops. Microbial activity of MV soil, probably stimulated by the rhizodepositions of the natural vegetation, did not suffer from the osmotic effect due to the raising of soil solution ionic strength. Microbial quotient could be ranked as MV > PY > DR, whereas the metabolic quotient had an opposite trend. Thus, both quotients suggested that the interruption of PG amendment was not favouring microbial activity. Principal component analysis clearly identified the chemical and biochemical soil properties mostly affected by the reclamation and also indicated the longer PG amendment in PY plot. Stepwise discriminant analysis identified two physiologically different types of soil microflora, one less active present in the MV virgin soil and another more active present in the reclaimed PY and DR soils.  相似文献   

Outflow tract segmental arrhythmogenic right ventricular cardiomyopathy in an English Bulldog     

Roberto A. Santilli Med Vet  Luigi V. Bontempi Med Vet  Manuela Perego Med Vet  Lara Fornai Med Vet  Cristina Basso MD  PhD 《Journal of Veterinary Cardiology》2009,11(1):47-51

A 5-year-old English Bulldog was presented for acute onset of syncope and fatigue caused by sustained ventricular tachycardia with left bundle block morphology and inferior axis. This arrhythmia had the electrocardiographic features of a ventricular tachycardia arising from the right ventricular outflow tract (RVOT), as described in an experimental canine model and in people. Since a RVOT aneurysm was identified by echocardiography, a segmental form of arrhythmogenic right ventricular cardiomyopathy (ARVC) was suspected. Gross examination of the heart confirmed the bulging of the RVOT and histological examination of the ventricular myocardium revealed segmental involvement of the RVOT with transmural fibro-fatty degeneration. To the authors' knowledge, this is the first reported case of AVRC in an English Bulldog and the first example of segmental AVRC described in the dog.  相似文献   

Seven new aminoacyl sugars in Ipomoea batatas     

Dini I  Tenore GC  Trimarco E  Dini A 《Journal of agricultural and food chemistry》2006,54(16):6089-6093

An analysis of the polar extracts from sweet potato Ipomoea batatas (Convolvulaceae) led to the isolation of seven unknown aminoacyl sugars. On the basis of 1D, 2D NMR, and mass spectrometry data, the structures of the compounds were elucidated as: beta-D-fructofuranosyl-(2 --> 1)-alpha-D-[2-O-valyl]-glucopyranoside (1), beta-D-fructofuranosyl-(2 --> 1)-alpha-D-[2-O-tyrosyl]-glucopyranoside (2), beta-D-fructofuranosyl-(2 --> 1)-alpha-D-[2-O-threonyl]-glucopyranoside (3), beta-D-fructofuranosyl-(2 --> 1)-alpha-D-[2-O-hystidyl]-glucopyranoside (4), 2-beta-D-fructofuranosyl-(2 --> 1)-alpha-D-[2-O-alanyl]-glucopyranoside (5), beta-D-fructofuranosyl-(2 --> 1)-alpha-D-[2-O-tryptophanyl]-glucopyranoside (6), and beta-D-fructofuranosyl-(2 --> 1)-alpha-D-[2-O-glycyl]-glucopyranoside (7).  相似文献   

Genetic stability at nuclear and plastid DNA level in regenerated plants of <Emphasis Type="Italic">Solanum</Emphasis> species and hybrids     

Riccardo Aversano  Salvatore Savarese  Jose Maria De Nova  Luigi Frusciante  Maria Punzo  Domenico Carputo 《Euphytica》2009,165(2):353-361

In this work we detected the extent of variability at nuclear and cytoplasmic DNA level of regenerated plants belonging to Solanum genotypes with a different genetic background and somatic chromosome number. As for the nuclear characterization, a total of 66 (18.5%) polymorphic bands were scored using 13 ISSR primers on 45 randomly selected regenerants. Our results show that the regenerants obtained from clone cmm 1T and, at lower level, those from cph 1C are unstable under in vitro conditions or rather more prone to in vitro-induced stress leading to somaclonal variation than the other genotypes used. Two types of changes were observed: disappearance of parental ISSR fragments, termed “loss”; appearance of novel ISSR fragments, termed “gain”. The most frequent event occurring in the regenerants was the loss of fragments (41 bands). Regenerated plants were analyzed with seven plastid universal primers to determine the cytoplasmic composition at chloroplast level. All cpDNA primer pairs tested produced amplicons of the same size in all genotypes analyzed and no polymorphic fragments were observed with any universal primers used. Our results show that under in vitro culture conditions genotype affects the integrity of the genome. In addition, the absence of polymorphism at plastid level confirms the greater genetic stability of cytoplasmic DNA.  相似文献   

Positive PCR alone should not be considered sufficient to establish Dirofilaria repens as the cause of subcutaneous nodular lesions in the absence of a clear cytologic picture          下载免费PDF全文

Simone Manzocchi  Luigi Venco  Eleonora Piseddu  Nikola Pantchev  Carlo Masserdotti  Ugo Bonfanti  Francesco Albanese  Laura Helen Kramer  Walter Bertazzolo 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2017,46(3):389-390

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Climate change and oak growth decline: Dendroecology and stand productivity of a Turkey oak (<Emphasis Type="Italic">Quercus cerris</Emphasis> L.) old stored coppice in Central Italy     

Alfredo Di Filippo  Alfredo Alessandrini  Franco Biondi  Silvia Blasi  Luigi Portoghesi  Gianluca Piovesan 《Annals of Forest Science》2010,67(7):706-706

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• We combined stem volume increment analysis with dendroecological tools to address two unresolved issues concerning oak dieback in Mediterranean areas: early detection of changes in stand growth, and identification of mechanisms for observed growth declines.  相似文献    [首页] « 上一页 [1] [2] [3] [4] 5 [6] [7] [8] [9] [10] [11] 下一页 » 末  页»