Rabies virus infection in Aedes pseudoscutellaris cells: a study on receptorial structures     

L Seganti  F Superti  L Sinibaldi  M Marchetti  S Bianchi  N Orsi 《Comparative immunology, microbiology and infectious diseases》1991,14(3):265-275

Rabies virus is able to infect in vitro a wide range of homeothermic and poikilothermic cells but little is known about its multiplication in arthropod cells. In this research the infection of rabies virus in Aedes pseudoscutellaris cells, a mosquito cell line susceptible to mosquito-borne viruses, was studied. After 60 days of incubation at 26 degrees C up to 70% of infected cells showed the synthesis of both viral nucleocapsid and envelope antigens, although viral yield and cell damage could not be detected. Research performed in order to investigate the role of membrane carbohydrate moieties in rabies virus-mosquito cell interaction suggested the participation of galactose and N-acetylglucosamine whereas sialic acid, known to be a rabies binding site in many homeothermic cell lines, was not involved.  相似文献   

Prognostic value of echocardiographic indices of left atrial morphology and function in dogs with myxomatous mitral valve disease          下载免费PDF全文

Marco Baron Toaldo  Giovanni Romito  Carlo Guglielmini  Alessia Diana  Nazzareno G. Pelle  Barbara Contiero  Mario Cipone 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2018,32(3):914-921

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Terrestrial-marine continuum of sedimentary natural organic matter in a mid-latitude estuarine system     

Bravo  Carlo  Millo  Christian  Covelli  Stefano  Contin  Marco  De Nobili  Maria 《Journal of Soils and Sediments》2020,20(2):1074-1086

Journal of Soils and Sediments - Humic acids (HA) have several environmental roles, but are particularly important in aquatic environments, being recognized as redox active natural organic matter...  相似文献   

Variation in parasitoid attraction to herbivore-infested plants and alternative host plant cover mediate tritrophic interactions at the landscape scale     

Aartsma  Yavanna  Hao  Yueyi  Dicke  Marcel  van der Werf  Wopke  Poelman  Erik H.  Bianchi  Felix J. J. A. 《Landscape Ecology》2020,35(4):907-919

Landscape Ecology - Tritrophic interactions may be affected by local factors and the broader landscape context. At small spatial scales, carnivorous enemies of herbivorous insects use...  相似文献   

Cytologic features of hepatic fibrosis in dogs: a retrospective study on 22 cases          下载免费PDF全文

Carlo Masserdotti  Walter Bertazzolo 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2016,45(2):361-367

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Tolerance of benzalkonium chloride, formalin, malachite green, and potassium permanganate in goldfish and zebrafish     

Intorre L  Meucci V  Di Bello D  Monni G  Soldani G  Pretti C 《Journal of the American Veterinary Medical Association》2007,231(4):590-595

OBJECTIVE: To determine tolerance of goldfish and zebrafish to benzalkonium chloride, formalin, malachite green, and potassium permanganate. DESIGN: Tolerance study. ANIMALS: Adult goldfish (Carassius auratus) and zebrafish (Danio rerio). PROCEDURES: Groups of fish (n = 10/group) were exposed to each disinfectant at the therapeutic dosage; at 0.25, 0.5, 3, and 5 times the concentration used for the therapeutic dosage; and at the concentration used for the therapeutic dosage but for 3 or 5 times the recommended exposure time. RESULTS: In both species, exposure to malachite green at the therapeutic dosage resulted in toxic effects, including death. Exposure to formalin at the therapeutic dosage resulted in toxic effects in goldfish, but not zebrafish, and exposure to potassium permanganate resulted in toxic effects in zebrafish, but not goldfish. On the basis of the ratio of therapeutic dosage to median lethal dosage, in goldfish, formalin was more toxic than benzalkonium chloride, which was more toxic than malachite green, which was more toxic than potassium permanganate. In zebrafish, potassium permanganate was more toxic than formalin and benzalkonium chloride, which were approximately equally toxic and more toxic than malachite green. Extending treatment time increased the toxicity of potassium permanganate in zebrafish and the toxicity of formalin and malachite green in goldfish, but did not alter the toxicity of the other disinfectants. CONCLUSIONS AND CLINICAL RELEVANCE: Results indicated that there was no consistency between zebrafish and goldfish in their tolerance to disinfectants, and that therapeutic dosages reported in the literature for these disinfectants were not always safe.  相似文献   

Metastatic pericardial tumors in a dog with equivocal pericardial cytological findings     

Guglielmini C  Civitella C  Malatesta D  Palmieri C 《Journal of the American Animal Hospital Association》2007,43(5):284-287

A metastatic tumor associated with pericardial effusion was diagnosed in a 6-year-old, female, mixed-breed dog. Echocardiography identified multiple echogenic masses adherent to both visceral and parietal pericardium, while results of pericardial fluid cytology were non-diagnostic. The distribution pattern of the masses is remarkable in that they protruded from both pericardial surfaces, rather than one, and demonstrated an oscillatory motion during the cardiac cycle. Pathological examination confirmed the diagnosis of multiple metastatic tumors of the pericardium, with the primary tumor being an anaplastic gastric adenocarcinoma.  相似文献   

Current knowledge about the processes of luteolysis and maternal recognition of pregnancy in camelids     

Carolina Paula Bianchi  Maria F. Gallelli  Juan Manuel Herrera  Micaela A. Benavente  Liliana Rossetto  Marcelo Alfredo Aba 《Reproduction in domestic animals》2023,58(1):3-9

Camelids have many unique reproductive features that considerably differ from those of other domestic species. Females are induced ovulators with subsequent development of a corpus luteum (CL) with a short lifespan. Plasma progesterone concentration starts to increase on day 4, peaks on day 8–9 and, in non-pregnant animals, basal concentration is reached around day 10–11 post-induction of ovulation. Luteolytic pulses of prostaglandin F_2α (PGF_2α) are firstly detected on day 7 or 8 (approximately on day 5–6 after ovulation), with maximal luteolytic peaks observed between days 9 and 11 post-mating, in coincidence with a high endometrial expression of cyclooxygenase 2, a limiting enzyme in prostaglandins synthesis. Unlike other species, oxytocin seems not to be involved in the luteolytic process in these species. The CL is the main source of progesterone secretion, and its function is required to support pregnancy. Despite constant research efforts, aspects of reproduction and maternal recognition of pregnancy in camelids remain not fully understood. A transient decrease and subsequent recovery in plasma progesterone concentration are observed after day 9 post-mating in pregnant animals in association with a pulsatile release of PGF_2α and a transitory decrease in CL vascularization. Thus, embryo recognition should occur between days 8 and 12 post-mating. In camels, conceptus tissues exhibit aromatizing activity with the capacity to synthesize large amounts of oestradiol. Similarly, llama blastocysts secrete oestradiol-17β during the preimplantation stage, with a higher production during the elongation period. An increase in the endometrial expression of oestrogen receptor α is also observed on day 12 post-mating. All these evidences suggest that oestrogen could be the signal released by the embryo at the time of its recognition in camelids. Besides, nearly 98% of pregnancies are carried out in the left horn. A decrease in the endometrial expression of mucin 1 and 16 genes has been reported, suggesting that these changes are crucial for successful embryo implantation; however, no differences have been observed between horns. Thus, maternal recognition of pregnancy in camelids is a particularly complex process that must occur in a concise time to allow the rescue of the CL and embryo survival.  相似文献   

Prevalence and prognostic role of L wave and selected clinical and echocardiographic variables in dogs with atrial fibrillation     

Giovanni Romito  Stefana Darida  Carlotta Valente  Helen Poser  Barbara Contiero  Mario Cipone  Carlo Guglielmini 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2023,37(1):47-57

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Simultaneous determination of clofentezine,fenoxycarb and hexythiazox by HPLC on apples,pears and their pulps     

Carlo Bicchi  Angela D'Amato  Ivano Tonutti  Liliana Cantamessa 《Pest management science》1990,30(1):13-19

The simultaneous HPLC determination of clofentezine, fenoxycarb and hexythiazox in apples, pears and their pulps is described. The method is based on a clean-up procedure carried out on a Seppak C18 cartridge followed by HPLC analysis on an RP-18 column using acetonitrile + water as mobile phase. Average recoveries were 85.0% for clofentezine, 84.8% for fenoxycarb and 75.6% for hexythiazox on apple matrix; recoveries from pear matrix were in the same range. UV detection limits at 240 nm were 0.07 mg kg^?1 for clofentezine, 0.064 mg kg^?1 for fenoxycarb and 0.08 mg kg^?1 for hexythiazox.  相似文献