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71.
A sero-epidemiological survey of human and equine H3 influenza A virus infections in dogs and cats using the hemagglutination inhibition (HI) and neuraminidase inhibition (NI) tests was conducted. Serum samples were collected from 582 dogs and 237 cats in Japan during the periods 2002-2008 and 1997-2008, respectively. Although no HI antibodies against equine H3 virus were detected, 9 (3.8%) from cats and 12 (2.1%) from dogs were HI-positive against human H3 virus. Only one serum each from dogs and cats was NI-positive against N2 virus. These findings suggest that although equine H3 influenza virus infections have not been prevalent in companion animals, human H3N2 influenza A virus infections have occurred in dogs and cats in recent years in Japan.  相似文献   
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The innervation of acini and ducts of eccrine sweat glands by immunoreactive, vasoactive intestinal peptide-containing nerve fibers was sharply reduced in seven patients with cystic fibrosis compared to eight normal subjects. The decrease in innervation by this neuropeptide, which has been shown to promote blood flow and the movement of water and chloride across epithelial surfaces in other systems, may be a basic mechanism for the decreased water content and relative impermeability of the epithelium to chloride and other ions that characterize cystic fibrosis.  相似文献   
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A sand culture experiment was conducted to study the effect of saline water on the growth and fruit quality of processing tomato (Lycopersicon esculentum Mill.) Seedlings of five tomato cultivars were transplanted in quartz‐sand pots in a greenhouse at the Agricultural Experiment Station of Sultan Qaboos University. There were four saline nutrient solutions and a control consisting of half‐strength Hoagland solution. Salinity treatments were: 50 raM NaCl + 3 mM K2SO4 (EC 6.75), 50 mM NaCl + 1.5 mM orthophosphoric acid (EC = 7.18), 50 mM NaCl + 1.5 mM orthophosphoric acid + 3 mM R2SO4 (EC 7.29), and 50 mM NaCL (EC = 5.6). Treatments were applied daily commencing two weeks after transplanting. Data were collected on growth, and fruit yield and quality. Partitioning of mineral elements was determined in the vegetative tissue. The results obtained clearly show that concentrations of total soluble solids were increased in fruits treated with saline nutrients. Dry matter content of fruits exposed to salinity were higher than those from the control plants. Fruit acidity was increased with salinity, possibly due to a lower water content and increased organic acid accumulation. In the saline treatments, sodium (Na) content was decreased when potassium (K) was applied with NaCl but Na was higher in stems followed by root and leaf tissues. The partitioning of K followed a trend opposite to that for Na but with higher content in leaves. A similar situation was observed for calcium (Ca) and magnesium (Mg). Accumulation of phosphorus (P) was the lowest among all the ions. These results indicated that survival under saline conditions was accompanied by high ion accumulation. The study confirmed that saline nutrients are important for improving fruit quality of processing tomatoes.  相似文献   
76.
菜豆荚斑驳病毒的RT-PCR检测   总被引:1,自引:1,他引:1  
魏梅生  相宁  张春泉 《大豆科学》2005,24(4):317-319
菜豆荚斑驳病毒是对大豆经济具有重要影响的病毒.根据已报道的菜豆荚斑驳病毒外壳蛋白基因序列,设计出一对特异性引物,采用RT-PCR法,可有效地扩增出BPMV不同亚组的4个分离物,得到650 bp大小的PCR产物.这种方法能成功地检测出大豆病叶中的BPMV,结果和酶联检测是一致的,而对大豆种皮中BPMV的检测效果不佳,建议采用酶联的方法进行检测.  相似文献   
77.
Dairy proteins included sweet whey solids (SWS), whey protein concentrate (WPC), milk protein concentrate (MPC) or sodium caseinate (SC) at level of 10% by weight were extruded with corn, rice, oat flour and other ingredients in a twin screw extruder. The functional properties and textural of crispy balls snacks (CBS) have been studied. The results showed that different protein types were significantly affected on functional and chemical properties of extrudate. The bulk density of CBS-SC was the lowest due to the highest expansion, while CBS-WPC retained the most water in the product, whereas, the maximum value of water solubility index was obtained in the CBS-CWP. Results also showed that protein digestibility value of extrudates had higher than non-extruded products. Color analysis of CBS-SC and CBS-SWS products had the highest AE values (P ≤ 0.05), whereas CBS-SWS was statistically higher than CBS-SC in rank. Also, it was noticed that CBS-SWS had the highest value of browning index. The hardness of all extrudates products was desirable for expanded crispy where ranged from 17.3 N to 12.18 N. Generally, MPC and SC incorporated with cereal flour had acceptable quality characteristics and improved significantly the functional and texture properties of crispy balls products.  相似文献   
78.
Conservation tillage may improve yield of cotton in addition to improvement in soil quality if practiced for longer period. However, the practice may not be productive in short-term particularly when severe weeds are infesting the crops such as Cynodon dactylon, Conyza canadensis, Tribulus terrestris, and Cyperus rotundus, etc. Recent studies indicate that conventional tillage (CT) is more productive than zero tillage (ZT)/reduced tillage (RT). Performance of cotton under three tillage systems, viz., ZT, RT and CT; and five herbicides, i.e., haloxyfop-R-methyl 10.8 EC (108 g a.i. ha?1), lactofen 24 EC (168 g a.i. ha?1), haloxyfop 10.8 EC + lactofen 24 EC, hand weeding, and weedy check were evaluated during 2010-2011 at Gomal University, D.I. Khan, Pakistan, to explore the best management option for effective weed control, enhanced yield and quality of cotton grown after wheat. The results revealed that hand weeding and Haloxyfop as post emergence alone or in combination with Lactofen reduced weed density to the minimum irrespective of the tillage systems. Excessive rainfall and cooler temperature limited cotton growth and yield in 2010. The adverse weather conditions had more adverse effect on boll weight under ZT and RT than CT. Haloxyfop + lactofen produced higher seed cotton yield in RT than ZT, however, it could not exceed CT. Broad-spectrum herbicides × CT produced the highest number of bolls/plant, boll weight and seed cotton yield. Fiber quality and net returns were also the highest in broad-spectrum herbicides × CT. In conclusion, broad-spectrum herbicides under CT were more productive in wheat based cropping system on silty clay soil of D.I. Khan.  相似文献   
79.
The study was conducted to investigating the effect of α-tocopherol acetate on heat shock protein 70 (Hsp70), oxidative stress, and antioxidant status during periparturient period in medium body condition score crossbred cows. Twenty crossbred Karan Fries cows with confirmed pregnancy were selected 2 months before expected date of calving. The cows were randomly distributed in to two groups: 10 cows were kept as control and 10 were supplemented with α-tocopherol acetate during dry period for 2 months. Blood samples were collected at ?20, ?10, ?5, 0, 5, 10, and 20 days in relation to the expected date of calving. Superoxide dismutase, catalase, and total immunoglobulin were significantly higher (P?<?0.01) in treatment as compared to control cows. Heat shock protein 70 and thiobarbituric acid reactive substance levels were significantly lower (P?<?0.01) in the treatment cows than their counterpart. Treatment with α-tocopherol acetate during dry period resulted in reduced oxidative stress, heat shock protein Hsp70 levels, improved antioxidant, and improved immunity status indicating beneficial effect of α-tocopherol acetate treatment.  相似文献   
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