MicroRNA expression profiling of cat and dog kidneys     

Osamu Ichii  Saori Otsuka  Hiroshi Ohta  Akira Yabuki  Taro Horino  Yasuhiro Kon 《Research in veterinary science》2014

MicroRNAs (miRNAs) play a role in the pathogenesis of certain diseases and may serve as biomarkers. Here, we present the first analysis of miRNA expression in the kidneys of healthy cats and dogs. Kidneys were divided into renal cortex (CO) and medulla (MD), and RNA sequence analysis was performed using the mouse genome as a reference. A total of 277, 276, 295, and 297 miRNAs were detected in cat CO, cat MD, dog CO, and dog MD, respectively. By comparing the expression ratio of CO to MD, we identified highly expressed miRNAs in each tissue as follows: 41 miRNAs including miR-192-5p in cat CO; 45 miRNAs including miR-323-3p in dog CO; 78 miRNAs including miR-20a-5p in cat MD; and 11 miRNAs including miR-132-5p in dog MD. Further, the target mRNAs of these miRNAs were identified. These data provide veterinary medicine critical information regarding renal miRNA expression.  相似文献   

Rate of Sulfur Dioxide Removal Artificial Cloud Experiments Utilizing a Long Vertical Shaft     

Utiyama  M.  Fukuyama  T.  Yamagata  S.  Ohta  S.  Izumi  K.  Harimaya  T.  Fujiyoshi  Y.  Yamada  T.  Inage  M. 《Water, air, and soil pollution》2001,130(1-4):325-330

Water, Air, &; Soil Pollution - Removal of sulfur dioxide through the absorption into cloud droplets is the initial stage of acid rain formation. We studied this process by forming an artificial...  相似文献   

Allopatric distributions of tetraploid and hexaploid forms of Aegilops neglecta Req. ex Bertol.     

Shoji Ohta  Yoshiki Nosaka  Hidekazu Yamagata 《Genetic Resources and Crop Evolution》2016,63(2):193-197

The genus Aegilops L. comprises 22 annual wild species that are closely related to wheat (Hammer in Kulturpflanze 28: 33–180, 1980). Aegilops neglecta Req. ex Bertol. is a member of the Aegilops section of this genus and is distributed from Morocco and Spain in the west to Transcaucasia and western Iran in the east. This species includes tetraploid (2n = 28, genome UUMM) and hexaploid forms (2n = 42, UUMMNN). However, the geographical distributions of the two cytological forms remain unclear. Clarifying the distribution of the two cytological forms is essential for a better understanding of the diffusion of Ae. neglecta and its tetraploid and hexaploid forms. In the present study, chromosome numbers were determined for accessions of Ae. neglecta from a total of 137 populations, located in the western area of the species distribution from the Aegean Islands to Morocco. Taken together with previous studies, the present data reveal a difference in the geographical distribution of tetraploid and hexaploid forms: tetraploid form is distributed in the eastern part of the species area and hexaploid form predominantly occurs in the western part with their border on the western margin of the Aegean Sea. Near the border, tetraploid and mixed populations are sporadically found among hexaploid populations in the Balkan and Peloponnesus Peninsulas, while a few hexaploid and mixed populations are found among tetraploid populations in the East Aegean Islands and West Anatolia.  相似文献   

General Chemical Properties of Brown Forest Soils Developed from Different Parent Materials in the Submontane Zone of the Kanto and Chubu Districts, Japan   总被引：1，自引：0，他引：1  

Akihiro Imaya  Seiichi Ohta  Nagaharu Tanaka  Yoshiyuki Inagaki 《Soil Science and Plant Nutrition》2005,51(6):873-884

It is essential to analyze chemical properties including the amount of various materials and the soil colloid characteristics in forest soils to forecast wood production and the distribution of and variations in the environmental functions of forest soils, such as conservation of stream water and carbon sequestration. Approximately 70% of the forest soils in Japan consists of Brown Forest Soils (BFS), which are considered to be typical zonal soils under the humid-temperate and warm-temperate regime of Japan. BFS were subclassifled into several groups according to the soil moisture environment along the slope and morphological properties. However, even the same type of soil may display different properties depending on the climatic conditions, parent materials and vegetation types. In the present study, the variations in the carbon content, nitrogen content, cation exchange capacity and some properties depending on the parent materials, were clarified by using 34 sola of BFS, and 3 sola of black soils (BLS) for comparison, which were distributed in the submontane zone of the Kanto and Chubu districts in central Japan under the same climatic conditions. We observed differences in the pH, cation exchange capacity, base saturation and clay content among BFS samples derived from various parent materials. The BFS derived from volcanic ash contained obviously larger amounts of carbon and nitrogen than the BFS derived from other parent materials. However, the BFS derived from volcanic ash differed from the BLS derived from volcanic ash in the vertical distribution pattern of carbon and nitrogen. Thus, even in the submontane zone of the Kanto and Chubu districts, the chemical properties of BFS varied considerably with the parent materials. It was concluded that the classification of BFS by the parent materials was useful for evaluating the ability of the BFS, that cover 70% of the forests in Japan, to store various materials.  相似文献   

Behavior of nitrogen in the media supplied with the aqueous phase produced by methane fermentation as liquid fertilizer     

Hisatoshi MIYATA  Hideo IKEDA 《Soil Science and Plant Nutrition》2006,52(1):135-135

pp. 859–864
Behavior of nitrogen in the media was investigated when the aqueous phase produced by methane fermentation was supplied as liquid fertilizer.
When the aqueous phase was supplied to masa-soil,      included in masa-soil was nitrified to      . Additionally, in masa-soil,       absorption was observed. On the other hand, in the case of coconut fiber and rockwool,     has been hardly nitrified. From above results, it was determined that       toxic effect from the aqueous phase on tomato plants growth was inhibited by means of the progress of nitrification and       absorption by masa-soil.
  However, nitrification was restrained so that       was fixed in masa-soil. On the other hand, in the sampled field soil,       was not fixed, and was nitrified immediately. In the field soil, there was a great deal of generated      . In comparison with masa-soil.  相似文献   

Metabolism of polychlorinated dibenzo-p-dioxins (PCDDs) by human cytochrome P450-dependent monooxygenase systems     

Inouye K  Shinkyo R  Takita T  Ohta M  Sakaki T 《Journal of agricultural and food chemistry》2002,50(19):5496-5502

Metabolism of polychlorinated dibenzo-p-dioxins (PCDDs) by monooxygenase systems dependent on 12 forms of human cytochrome P450 (CYP) was examined with the recombinant yeast microsomes containing each of the human CYP. The metabolites of PCDDs were analyzed by HPLC and GC-MS. Remarkable metabolism by the multiple CYP forms was observed toward dibenzo-p-dioxin (DD) and mono-, di-, and trichloroDDs. The metabolism contained multiple reactions such as hydroxylation at an unsubstituted position, hydroxylation with migration of a chloride substituent, and hydroxylation with elimination of a chloride substituent. Although major CYPs in human liver such as CYP2C8, CYP2C9, and CYP3A4 showed no significant metabolism toward the PCDDs, CYP1A1 and CYP1A2 showed high catalytic activity toward DD and mono-, di-, and trichloroDDs. The kinetic parameters K(m)(app) and V(max) of the CYP1A1-dependent 8-hydroxylation activity of 2,3,7-trichloro-DD (2,3,7-triCDD) were estimated to be 0.30 microM and 51 (mol/min/mol of P450), respectively, suggesting that 2,3,7-triCDD was a good substrate for CYP1A1. However, none of the CYPs showed any detectable activity [<0.01 mol/min/mol of P450)] toward 2,3,7,8-tetraCDD. Substrate-induced absorption spectrum and inhibition studies indicated that CYP1A1 could bind 2,3,7,8-tetraCDD with considerably high affinity. It was strongly suggested that the long half-life (7.1 years) of 2,3,7,8-tetraCDD in humans was due to an extremely low activity of CYPs toward 2,3,7,8-tetraCDD in addition to its chemical stability.  相似文献   

Clinical characteristics of dogs with food-responsive protein-losing enteropathy     

Noriyuki Nagata  Hiroshi Ohta  Nozomu Yokoyama  Yong Bin Teoh  Khoirun Nisa  Noboru Sasaki  Tatsuyuki Osuga  Keitaro Morishita  Mitsuyoshi Takiguchi 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2020,34(2):659-668

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Cryopreservation diluents for spermatozoa of Sakhalin taimen <Emphasis Type="Italic">Hucho perryi</Emphasis>     

Satoshi?KusudaEmail author  Nobuhisa?Koide  Hiloshi?Kawamula  Tetsuo?Teranishi  Jun?-Ichiro?Nakajima  Etsuro?Yamaha  Katsutoshi?Arai  Hiromi?Ohta 《Fisheries Science》2005,71(2):293-298

To develop a suitable cryopreservation diluent for spermatozoa of the endangered Sakhalin taimen Hucho perryi, all possible combinations of cryoprotectants (glycerol, dimethyl sulfoxide [DMSO], methanol) and extenders (fetal bovine serum [FBS], 300 mM glucose solution [GS], artificial seminal plasma for masu salmon) were examined by observing sperm motility 10 s after thawing. Spermatozoa cryopreserved with diluents such as mixtures of 10% glycerol plus 90% FBS, 10% DMSO plus 90% FBS, and 10% methanol plus 90% GS showed the highest motility. The maximal post-thaw motility was observed at 10% among all concentrations (0, 5, 10, 15 and 20%) of these three cryoprotectants. No significant difference among three diluents was observed in motility at 10 s. Mixtures of 10% glycerol plus 90% FBS, 10% DMSO plus 90% FBS, and 10% methanol plus 90% GS are suitable cryopreservation diluents for Sakhalin taimen spermatozoa.  相似文献   

The first production of glass eel in captivity: fish reproductive physiology facilitates great progress in aquaculture     

H. Tanaka  H. Kagawa  H. Ohta  T. Unuma  K. Nomura 《Fish physiology and biochemistry》2003,28(1-4):493-497

Weekly injections of salmon pituitary extracts (SPE) were administered to female Japanese eel, Anguilla japonica at a dose of 20 mg/fish. This induced vitellogenesis and caused oocytes to reach the migratory nucleus stage. Later, a majority of the females that received an injection of SPE at a priming dose, followed 24 h later by 17,20β-dihydroxy-4-pregnen-3-one (DHP), ovulated 15 to 18 h after the final injection. In cultivated males, repeated injections of human chorionic gonadotropin (hCG) at a dose of 1 IU/g BW/week induced spermatogenesis and spermiation. Since potassium ions were revealed to be an essential constituent for the maintenance of motility in the eel spermatozoa, artificial seminal plasma containing KCl was designed as a diluent of milt, and enabled the preservation of milt for several weeks in refrigeration. As a result, artificial fertilization performed immediately after ovulation with pre-diluted and stocked milt consistently resulted in the production of high-quality gametes. Recently, a slurry-type diet made from shark egg yolk has been found to be a suitable feed for captive-bred eel larvae. Although preleptocephalus larvae can be reared with this diet beyond the depletion of their yolk and oil droplet stores, it remains inadequate because larvae reared under this way cannot be raised to the following stage. Therefore, the diet was improved by supplements of krill hydrolysate, soybean peptide, vitamins and minerals. Larvae fed on this new diet have grown to 50 to 60 mm in total length (TL), and have begun to metamorphose into glass eels approximately 250 days after hatching.  相似文献   

Evaluation of end-check propagation based on mode I fracture toughness of sugi (<Emphasis Type="Italic">Cryptomeria japonica</Emphasis>)     

Ken Watanabe  Satoshi Shida  Masamitsu Ohta 《Journal of Wood Science》2011,57(5):371-376

The relation between crack propagation based on fracture mechanics and end-check propagation during drying was evaluated in this study. Corresponding to the direction of end-check propagation, the mode I fracture toughness of air-dried sugi specimens in TR, TL, and intermediate systems was examined by single-edge-notched bending tests. The occurrence and propagation of end checks on sugi (Cryptomeria japonica D. Don) blocks during drying were observed at the scale of the annual rings. It was found that the critical stress intensity factor (K _IC) decreased as the crack propagation changed from TL to TR. The value of K _IC in the TR system was significantly lower than that in the TL system. As a measure of fracture energy, the area under the load-crack opening displacement curve in the TR system was more than twice that in the TL and intermediate systems. These results indicate that cracks perpendicular to the tangential direction initiate radially with ease, and then crack arrest occurs to prevent growing. This finding provides a consistent interpretation of the end-check propagation observed during drying as follows: tiny end checks, as an analog of TR cracks, occur easily and selectively in latewood or transition wood and propagate toward the pith during drying. When there is no corresponding secondary check in the forward latewood, the checks are arrested and do not propagate further.  相似文献