Cryopreservation diluents for spermatozoa of Sakhalin taimen <Emphasis Type="Italic">Hucho perryi</Emphasis> |
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| Authors: | Email author" target="_blank">Satoshi?KusudaEmail author Nobuhisa?Koide Hiloshi?Kawamula Tetsuo?Teranishi Jun?-Ichiro?Nakajima Etsuro?Yamaha Katsutoshi?Arai Hiromi?Ohta |
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| Institution: | (1) Hokkaido Fish Hatchery, 061-1433 Eniwa, Hokkaido, Japan;(2) Graduate School of Fisheries Sciences, Hokkaido University, 041-8611 Hakodate, Hokkaido, Japan;(3) Nanae Fresh-Water Laboratory, Field Science Center for Northern Biosphere, Hokkaido University, 041-1105 Nanae, Hokkaido, Japan;(4) Laboratory of Aquaculture, Department of Fisheries, Faculty of Agriculture, Kinki University, 631-8505 Nara, Japan |
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| Abstract: | To develop a suitable cryopreservation diluent for spermatozoa of the endangered Sakhalin taimen Hucho perryi, all possible combinations of cryoprotectants (glycerol, dimethyl sulfoxide DMSO], methanol) and extenders (fetal bovine
serum FBS], 300 mM glucose solution GS], artificial seminal plasma for masu salmon) were examined by observing sperm motility
10 s after thawing. Spermatozoa cryopreserved with diluents such as mixtures of 10% glycerol plus 90% FBS, 10% DMSO plus 90%
FBS, and 10% methanol plus 90% GS showed the highest motility. The maximal post-thaw motility was observed at 10% among all
concentrations (0, 5, 10, 15 and 20%) of these three cryoprotectants. No significant difference among three diluents was observed
in motility at 10 s. Mixtures of 10% glycerol plus 90% FBS, 10% DMSO plus 90% FBS, and 10% methanol plus 90% GS are suitable
cryopreservation diluents for Sakhalin taimen spermatozoa. |
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| Keywords: | cryopreservation cryoprotectant extender Hucho perryi Sakhalin taimen spermatozoa |
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