Ko Minderhout steps down as chairman. Interview by Miel Bingen     

Minderhout K 《Tijdschrift voor diergeneeskunde》2008,133(19):824

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A comparison of two combinations of xylazine-ketamine administered intramuscularly to alpacas and of reversal with tolazoline     

Prado TM  DuBois WR  Ko JC  Mandsager RE  Morgan GL 《Veterinary anaesthesia and analgesia》2008,35(3):201-207

ObjectiveTo evaluate the anesthetic and cardiorespiratory effects of two doses of intramuscular (IM) xylazine/ketamine in alpacas, and to determine if tolazoline would reduce the anesthetic recovery time.Study designProspective randomized crossover study.AnimalsSix castrated male alpacas.MethodsEach alpaca received a low dose (LD) (0.8 mg kg⁻¹ xylazine and 8 mg kg⁻¹ ketamine IM) and high dose (HD) (1.2 mg kg⁻¹ xylazine and 12 mg kg⁻¹ ketamine IM) with a minimum of one week between trials. Time to sedation, duration of lateral recumbency and analgesia, pulse rate, respiratory rate, hemoglobin oxygen saturation, arterial blood pressure, blood-gases, and the electrocardiogram were monitored and recorded during anesthesia. With each treatment three alpacas were randomly selected to receive tolazoline (2 mg kg⁻¹ IM) after 30 minutes of lateral recumbency.ResultsOnset of sedation, lateral recumbency and analgesia was rapid with both treatments. The HD was able to provide ≥30 minutes of anesthesia in five of six alpacas. The LD provided ≥30 minutes of anesthesia in three of six alpacas. Respiratory depression and hypoxemia occurred with the HD treatment during the first 10 minutes of lateral recumbency: two animals were severely hypoxemic and received nasal oxygen for 5 minutes. Heart rate decreased, but there were no significant changes in arterial blood pressure. Tolazoline significantly shortened the duration of recumbency with the HD.ConclusionsThe HD provided more consistent clinical effects in alpacas than the LD. Intramuscular tolazoline shortened the duration of lateral recumbency in alpacas anesthetized with the HD combination.Clinical relevanceBoth doses of the combination were effective in providing restraint in alpacas and the duration of restraint was dose dependent. Supplemental oxygen should be available if using the HD and IM administration of tolazoline will shorten the recovery time.  相似文献   

Effect of dietary nutmeg oil on heat‐stress tolerance‐related parameters in Korean native chicken reared under hot temperature     

Slamet Hartanto  Han Seo Ko  Seung Hwan Jee  Ji Ung Kang  Jee Soo Seo  Yu Hyun Kang  Hee Na Kim  Sang Jip Ohh 《Journal of animal physiology and animal nutrition》2019,103(4):1160-1167

This study investigated the effect of dietary nutmeg oil (NO) on growth performance, blood parameters, lipid peroxidation and heat shock protein (HSP) 70 expression in Korean native chicken (KNC) reared under hot temperature. We allocated 273 meat‐type KNCs (Hanhyup‐3, 4‐week‐old, body weight [BW] = 539.93 ± 1.75 g) to the following three treatments with seven replicate pens (13 birds/pen) per treatment. Three treatment diets were as follows: (a) Control, basal diet without NO supplementation; (b) NO 250; and (c) NO 500, basal diet supplemented with 250 and 500 ppm NO respectively. Diets and water were provided ad libitum throughout the 6‐week feeding trial. During overall period (0–6 weeks), no differences (p > 0.05) were observed in BW gain (BWG), feed intake (FI) and feed conversion rate (FCR) among treatments. However, the FI at 0–3 weeks decreased (p < 0.05) quadratically with increasing NO levels. Most blood parameters did not differ (p > 0.05) among treatments, although the monocyte level of the NO 500 group was considerably lower (p > 0.05) than that of the other groups. Furthermore, dietary NO did not affect serum triglyceride, cholesterol, total protein, albumin, calcium, phosphorus and alanine aminotransferase (ALT) levels (p > 0.05); however, it linearly decreased serum aspartate aminotransferase (AST) level (p < 0.05). Additionally, serum malondialdehyde (MDA) concentration decreased (p < 0.05) and heart MDA concentration was lower (p = 0.08) with increasing dietary NO supplementation. After a 3‐hr heat (35°C) challenge, the rectal temperature (RT) reduced (p < 0.05) linearly with increasing NO levels. Dietary NO did not affect liver HSP70 (p > 0.05) gene expression. In conclusion, NO potentially enhanced the ability of chickens to alleviate heat stress. Furthermore, our findings suggest that lipid oxidation inhibition by dietary NO likely mediated the enhanced heat‐stress tolerance of the chickens.  相似文献   

Impact of bacterial translocation in calves with atresia coli          下载免费PDF全文

Semih Altan DVM  PhD  Yılmaz Koç DVM  PhD  Fahrettin Alkan DVM  PhD  Zafer Sayın DVM  PhD  Muharrem Erol DVM  PhD 《Journal of Veterinary Emergency and Critical Care》2018,28(3):261-268

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Effects of the Administration of Lactobacilli,Maltodextrins and Fructooligosaccharides upon the Adhesion of <Emphasis Type="Italic">E. coli</Emphasis> O8:K88 to the Intestinal Mucosa and Organic Acid Levels in the Gut Contents of Piglets     

Nemcová R  Bomba A  Gancarcíková S  Reiffová K  Guba P  Koscová J  Jonecová Z  Sciranková L  Bugarský A 《Veterinary research communications》2007,31(7):791-800

The influence of the administration of Lactobacillus plantarum, maltodextrin Maldex 150 and Raftifeed IPX fructooligosaccharides on the inhibition of adhesion of E. coli O8:K88 to the mucosa of the jejunum, ileum and colon as well as on the organic acid levels was investigated in 33 conventional piglets. The counts of E. coli K88 adhering to the jejunal mucosa were significantly decreased (p < 0.05) in Lact. plantarum + Maldex 150 and Lact. plantarum + Maldex 150 + Raftifeed IPX groups. The counts of E. coli K88 adhering to the colonic mucosa of Lact. plantarum + Maldex 150 + Raftifeed IPX and Lact. plantarum + Raftifeed IPX groups were significantly lower (p < 0.05) than in Lact. plantarum and Lact. plantarum + Maldex 150 animals. The acetic acid levels in the ileum and colon of the Lact. plantarum + Maldex 150 + Raftifeed IPX group and Lact. plantarum + Raftifeed IPX group were significantly higher (p < 0.05) than in the Lact. plantarum and Lact. plantarum + Maldex 150 group. The combination of Lact. plantarum, maltodextrin Maldex 150 and Raftifeed IPX proved to be the most effective one to inhibit the counts of E. coli O8:K88 adhering to the intestinal mucosa of the jejunum and colon of conventional piglets.  相似文献   

New sampler for dry deposition in forest canopy     

Chisato Takenaka  Win Ko Ko 《Journal of Forest Research》1998,3(1):19-24

A new type of sampler for dry deposition in a forest canopy was designed and tested to confirm its usefulness. The new sampler is made of Teflon chips packed in a nylon net, and tied on a rod like a branch in the canopy. The validity and characteristics of the new sampler were checked by comparing it with the conventional bucket sampler and the foliar extraction method using hinoki (Chamaecyparis obtusa) trees. The comparison between the deposited amounts on the dummy branch and those on the bucket indicates that the dummy could trap gaseous matter more effective than the bucket. From a comparison with the foliar extracting method, it is concluded that the trapping of particulate matter by the new dummy branch is comparable to that of natural needle leaves. However, its efficiency for gaseous matter is relatively small. This disadvantage should be improved by applying other materials to the dummy. Moreover, it is found that the uptake of gas especially SO₂ through the stomata is an important point in the discussion about dry deposition. To evaluate the amounts of dry deposition in the forest canopy more precisely, combination with the additional method should be useful. This study was supported in part by a grant-in-aid for Scientific Research from the Ministry of Education, Science, Sports, and Culture, Japan.  相似文献   

Herbicidal and biological characteristics of a new benzenesulfonylurea compound K-11451 under submerged paddy conditions     

In Taek Hwang  Kyung Sik Hong  Jung Sup Choi  Jin-Seog Kim  Ju Hyun Yu  Young Kwan Ko  Jae Wook Ryu  Hae Sung Chang  Dae-Whang Kim  Kwang Yun Cho 《Pest management science》1999,55(5):578-580

The new benzenesulfonylurea K-11451, an α-hydroxy-β-fluoropropyl- compound, applied at 3–9 g ha ⁻¹, controlled annual and perennial weeds grown in submerged paddy soil under greenhouse conditions. It effectively controlled barnyardgrass at growth stages varying from pre-emergence to the five-leaf stage. The compound inhibited acetolactate synthase, I₅₀ values for the enzyme isolated from barnyardgrass and rice being 56 and 67 nM , respectively. K-11451 inhibited the growth of rice when it was transplanted at a shallow depth (0–1 cm) and water leaching from the paddy soil was high (3–5 cm per day). With a water depth of 3 cm, the compound appeared to move readily down into the paddy soil and had the relatively short half-life of 15.2 days under submerged paddy conditions. A mixture of K-11451 + mefenecet + daimuron (9 + 250 + 250 g ha⁻¹) controlled almost all weeds in the paddy field without injury to rice, so that the combination could be used as a ‘one-shot’ herbicide in rice culture.  相似文献   

Influence of protein characteristics and the proportion of gluten on end-use quality in Korean wheat cultivars     

Seong-Woo Cho  Chon-Sik Kang  Hyeon Seok Ko  Byung-Kee Baik  Kwang-Min Cho  Chul Soo Park 《农业科学学报》2018,17(8):1706-1719

The effects of protein characteristics and the proportion of gluten on end-use quality in 13 Korean wheat cultivars for three years were verified in this study. Year, cultivar, and the interaction between the year and the cultivar influenced protein characteristics, the proportion of gluten except for γ-and ω-gliadin using RP-HPLC(reversed-phase high-performance liquid chromatography), and end-use quality. Protein characteristics and the proportion of gluten in Korean wheat cultivars were between those of Australian standard white(ASW) and hard wheat(AH). Korean wheat cultivars exhibited a higher average α+β gliadin proportion than imported wheat, a γ-gliadin proportion similar to that of dark northern spring wheat, and the same ω-gliadin proportion as AH. They showed a bread-loaf volume intermediate between those of ASW and AH and a texture of cooked noodles similar to that of soft white wheat, but less springiness than imported wheat. The cookie diameter of Korean wheat cultivars was similar to that of hard red winter wheat. There was a correlation between bread-loaf volume and protein characteristics, except for the protein content in Korean wheat cultivars. Springiness and cohesiveness of cooked noodles were not correlated with protein characteristics, while hardness was correlated with the protein content and water absorption of a mixograph. Cookie diameter was negatively correlated with the sodium dodecyl sulfate(SDS) sedimentation volume and water absorption of a mixograph. The end-use quality was not correlated with any proportion of gluten composition. Principal component analysis(PCA) showed that the proportion of gluten was not related to the quality of the bread(both PCs, 81.3%), noodle(77.7%), and cookie(82.4%). PCA explained that Keumkang is suitable for superior bread, while Uri is good for cooked noodles and cookies.  相似文献   

Metagenomic analysis using 16S ribosomal RNA genes of a bacterial community in an urban stream,the Tama River,Tokyo     

Md. Shaheed Reza  Nanami Mizusawa  Ayaka Kumano  Chiharu Oikawa  Daisuke Ouchi  Atsushi Kobiyama  Yuichiro Yamada  Yuri Ikeda  Daisuke Ikeda  Kazuho Ikeo  Shigeru Sato  Takehiko Ogata  Toshiaki Kudo  Mitsuru Jimbo  Ko Yasumoto  Kazutoshi Yoshitake  Shugo Watabe 《Fisheries Science》2018,84(3):563-577

In an effort to determine genus- or species-level taxonomic profiles and diversity of bacterial consortia in the Tama River around urban Tokyo, next-generation sequencing technology targeting a 16S ribosomal RNA (rRNA) gene amplicon was employed. Metagenomic analysis performed by an Ion Personal Genome Machine after sequentially filtering samples through 5-, 0.8- and 0.2-μm filters yielded 1.48 Gb of 16S sequences (average 2.38 M reads/sample). The results indicated that half of the bacterial sequences belonged to Proteobacteria, followed by Bacteroidetes, Actinobacteria and Cyanobacteria. Flavobacterium (Bacteroidetes), possibly including a potential fish pathogen, was the most numerous genera in the Tama River metagenome, and accounted for?~?16% of assigned 16S reads, followed by Mycobacterium. Other dominant bacterial genera including Zoogloea, Sediminibacterium, Hyphomicrobium, Sphingopyxis, Thiothrix and Lysobacter, were thought to be associated with waste water and sludge. MiSeq metagenomic analysis revealed that environmental factors, particularly water temperature, influenced the bacterial composition throughout the year, with a strong negative correlation observed for Proteobacteria and a positive correlation for Bacteroidetes. In terms of bacterial genera, Flavobacterium was positively correlated with temperature, while Polaromonas, Pseudomonas and Bradyrhizobium were negatively correlated with this, suggesting dynamic change in the free-living bacterial population throughout the year and versatile adaptation strategies in relation to environmental factors.  相似文献   

Habitat use and movement patterns of small (age‐0) juvenile Pacific bluefin tuna (Thunnus orientalis) relative to the Kuroshio          下载免费PDF全文

Ko Fujioka  Hiromu Fukuda  Seishiro Furukawa  Yaoki Tei  Suguru Okamoto  Seiji Ohshimo 《Fisheries Oceanography》2018,27(3):185-198

The habitat use of Pacific bluefin tuna (Thunnus orientalis; PBF) in nursery waters off the southern coast of Japan was investigated using archival tags over a 3 year study period (2012–2015), and the data were used to examine the free‐ranging habitat preferences of PBF and the relationship between their horizontal movements and the path of the Kuroshio off the Pacific coast of Japan. The path of the Kuroshio fluctuated seasonally, leading to changes in water temperature that strongly influenced the habitat use of small PBF (2–3 months after hatching). Most PBF were present in coastal waters inshore of the path of the current, and their habitat use changed in response to the distance of the current from the coast. The Kuroshio typically flowed along the coast from summer to autumn, and PBF remained in the coastal waters off Kochi Prefecture during this period. In contrast, PBF quickly moved eastward in winter when the current moved away from the coast. Throughout the winter and spring, the area of habitat use extended widely from the eastern end of the southern coast of Japan (the Boso Peninsula) to the offshore Kuroshio‐Oyashio transition region. These findings suggest that the seasonal habitat use and movement behavior of juvenile PBF are influenced by the distance of the Kuroshio axis from the coast, and the ultimate drivers are likely variations in oceanographic conditions and prey availability along the southern coast of Japan.  相似文献