血清中不同甘露(聚)糖结合凝集素浓度的绵羊感染绵羊肺炎支原体后免疫因子表达的变化     

古丽汗·阿不都热木  赵宗胜  李洪涛  王小义  杨恒 《中国预防兽医学报》2015,37(1)

为研究中国美利奴羊不同甘露(聚)糖结合凝集素(MBL)浓度感染绵羊肺炎支原体(MO)的免疫因子水平变化,本研究选择血清中MBL高、低浓度的绵羊各6只(感染组和对照组各3只),感染组人工感染MO,分别在人工感染前和感染后不同时间,采用荧光定量PCR法检测血液中血清因子及补体表达水平。结果显示,不同MBL浓度的绵羊人工感染后MBL m RNA水平呈下降趋势,MBL高浓度促炎因子IL-2和IFN-γ的m RNA表达水平较高,抗炎因子IL-4的m RNA表达水平在感染后1 d升高,此后开始下降,而IL-4的m RNA水平在14 d后有所升高;MBL低浓度羊感染后其TNF-α的m RNA水平显著升高,随炎症的缓解,逐渐降低;补体C1和C3的m RNA在感染后表现出不同的变化,MO感染可以激活补体途径。本研究结果表明,低血清MBL浓度与绵羊支原体肺炎具有一定的相关性,MBL不同浓度组之间其IL-2、IL-4、TNF-α、IFN-γ、补体C1、C3水平存在差异,低浓度MBL的绵羊更易发生比较严重的炎症反应。  相似文献   

Expression Pattern of Apoptotic Genes in Vitrified‐Thawed Bovine Oocytes     

VM Anchamparuthy  RE Pearson  FC Gwazdauskas 《Reproduction in domestic animals》2010,45(5):e83-e90

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Detection of calves persistently infected with bovine pestivirus in a sample of dairy calves in south-eastern Queensland     

RE BOCK  BJ RODWELL  M. McGOWAN 《Australian veterinary journal》1997,75(9):656-659

Objective To determine the proportion and incidence of calves persistently infected with bovine pestivirus in calves (n = 1521) supplied to the Tick Fever Research Centre and to assess the test regime to detect calves persistently infected with bovine pestivirus.
Design Calves, 1 to 6 weeks old, selected for use in the production of the tick fever vaccine were collected from 21 properties in 56 separate groups between October 1990 and December 1996. Each group was examined for the presence of calves persistently infected with bovine pestivirus.
Procedure All calves were routinely tested for antibody to bovine pestivirus and bovine pestivirus antigen using a serum neutralisation test and an antigen-capture ELISA, respectively. Pooled lymphocyte samples from calves were also monitored for bovine pestivirus by inoculation of sheep. Whole herd testing was carried out in eight herds, using a serum neutralisation test as a screen test followed by an antigen-capture ELISA of cattle with a serum neutralisation test titre of less than 32.
Results Fourteen of the 1521 calves tested (0.9%), were detected as persistently infected and the incidence ranged from 0.0 to 3.0 % per year over 6 years. Persistently infected calves were found in 13 of the 59 groups and originated from 7 of the 21 herds used. In whole herd testing on the properties of origin, cattle persistently infected with bovine pestivirus were detected in four of the eight herds tested
Conclusions The proportion of calves persistently infected with bovine pestivirus is similar to that in other countries and indicates that bovine pestivirus could be a significant cause of economic loss in Australian cattle herds. In detecting calves persistently infected with bovine pestivirus, the combination of sheep inoculation, paired antigen-capture ELISA and serum neutralisation tests appeared to be highly sensitive and specific.  相似文献   

兰州市生态农业旅游开发   总被引：4，自引：1，他引：4  

热娜 《安徽农业科学》2006,34(23):6380-6380,6382

系统分析了兰州市发展生态农业旅游的有利条件、开发现状和存在问题,提出了发展兰州市生态农业旅游的建议。  相似文献   

Presentation and first applications of a dynamic population model for brown trout, Salmo trutta L.: aid to river management     

C. SABATON  L. SIEGLER  V. GOURAUD  J.L. BAGLINIÈRE  & S. MANNÉ 《Fisheries Management and Ecology》1997,4(6):425-438

A mathematical model representing the long-term change in a trout population under different river management scenarios is presented. It describes the structure of a population broken down into age classes based on the Leslie matrix; if the population structure for any given month is known, the model should be able to estimate that of the following month. The passage from one month to the next takes into account various relevant factors: survival rate of individuals in the different age classes; fertility rate of females; linear and weighted growth rates; displacement linked to habitat fluctuations using weighted usable area (WUA) values. The model was applied to two French rivers. Regular monitoring of trout populations on the River Kernec enabled comparison of the response of the model with no displacement, with actual variations in fish stocks on the first river. In addition, the knowledge of WUA chronologies on the River Echez made it possible to carry out initial simulations of the response of a fish population to different river management scenarios at the second site.  相似文献   

Direct measurement of the current-phase relation of a superfluid 3He-B weak link     

S Backhaus  SV Pereverzev  A Loshak  JC Davis  RE Packard 《Science (New York, N.Y.)》1997,278(5342):1435-1438

Direct measurements of the current-phase relation, I versus Deltaphi, for a weak link coupling two reservoirs of B-phase superfluid helium-3 (3He-B) were made over a wide range of temperatures. The weak link consists of a square array of 100-nanometer-diameter apertures. For temperatures T such that T/Tc >/= 0.6 (where Tc is the superfluid transition temperature), I approximately sin(Deltaphi). At lower temperatures, I(Deltaphi) approaches a straight line. Several remarkable phenomena heretofore inaccessible to superconducting Josephson junctions, including direct observation of quantum oscillations and continuous knowledge of Deltaphi, were also observed.  相似文献   

新疆甜菜高产高效益优化农艺措施     

聂文魁  王燕飞  杨洪泽  刘华君  刘军  高卫时  张立明  热西提 《中国糖料》2002,(4):18-20

采用3因素5水平二次通用旋转回归试验设计方法,选取密度、种肥量、追肥量3项对甜菜高产、高效益起关键作用的农艺措施进行量化分析,提出直播方式下的高产、高效益模型,获得了60t/hm2以上和净收益7000元/hm2以上的优化农艺措施组合方案为:密度8.42～8.34万株/hm2,种肥222～248.4kg/hm2,追肥383～408.6kg/hm2。  相似文献   

The use of ultrasonography in the reproductive evaluation of boars          下载免费PDF全文

RO Pinho  BS Camilo  DMA Lima  FAC Villadiego  JCM Vergara  HH Shiomi  RE Cardoso  PS Lopes  JD Guimarães 《Reproduction in domestic animals》2018,53(2):393-400

The objective was to study the use of ultrasound as a complementary test in the breeding soundness evaluation in male pigs and study the pattern of echogenicity of the testicular parenchyma in boars of different racial groups. Twenty‐six adult boars from four different racial groups were used, 10 from the Piau breed (group 1), four from the commercial and finishing group (group 2), six Pietrain breed (group 3) and six from the Duroc breed (group 4). All animals were evaluated for breeding soundness evaluation and the ultrasound examination of the testicles. The groups of animals that were evaluated showed no difference in the main semen parameters that were evaluated, except for the sperm volume, concentration of the ejaculated sperm and the supravital staining; the lowest figures were for the animals from the Piau breed (group 1). In relation to the testicular biometrics, Duroc animals (group 4) had a greater scrotal width compared to the other groups. But when we assessed the intensity of pixels of the testicles, there was a difference between groups. The groups 2 (finishing animals), 3 (Pietrain) and 4 had no difference between themselves. Group 3 had greater pixel intensity in relation to group 1. Of the 26 animals studied, five showed an abnormality during ultrasound evaluation, like hydrocele, hyperechoic mass in the testicular parenchyma, cyst in the head of the epididymis and the presence of fluid in the head and tail of the epididymis. The various animal groups studied did not differ in the principal reproductive parameters evaluated, showing that despite the great variability of reproductive traits between breeds and within the same breed, the breeding soundness evaluation, the more complete it is, is essential for the selection of breeders and the ultrasonography of the reproductive system becomes an important addition in this examination.  相似文献   

Analgesic efficacy of an oral transmucosal spray formulation of meloxicam alone or in combination with tramadol in cats with naturally occurring osteoarthritis          下载免费PDF全文

Beatriz P Monteiro  Mary P Klinck  Maxim Moreau  Martin Guillot  Paulo VM Steagall  Daniel K Edge  Jean‐Pierre Pelletier  Johanne Martel‐Pelletier  Dominique Gauvin  Jérôme RE del Castillo  Eric Troncy 《Veterinary anaesthesia and analgesia》2016,43(6):643-651

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Assessment of tetracycline and Babesia culture supernatant as prophylactics for moderating reactions in cattle to live Babesia and Anaplasma vaccines     

WK JORGENSEN  RE BOCK  TG KINGSTON  AJ DE VOS  SJ WALDRON 《Australian veterinary journal》1993,70(1):35-36

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