Regulatory role of l-proline in fetal pig growth and intestinal epithelial cell proliferation     

Jing Wang  Bi'e Tan  Jianjun Li  Xiangfeng Kong  Minjie Tan  Guoyao Wu 《动物营养（英文）》2020,6(4):438

l-proline (Pro) is a precursor of ornithine, which is converted into polyamines via ornithine decarboxylase (ODC). Polyamines plays a key role in the proliferation of intestinal epithelial cells. The study investigated the effect of Pro on polyamine metabolism and cell proliferation on porcine enterocytes in vivo and in vitro. Twenty-four Huanjiang mini-pigs were randomly assigned into 1 of 3 groups and fed a basal diet that contained 0.77% alanine (Ala, iso-nitrogenous control), 1% Pro or 1% Pro + 0.0167% α-difluoromethylornithine (DFMO) from d 15 to 70 of gestation. The fetal body weight and number of fetuses per litter were determined, and the small and large intestines were obtained on d 70 ± 1.78 of gestation. The in vitro study was performed in intestinal porcine epithelial (IPEC-J2) cells cultured in Dulbecco''s modified Eagle medium-high glucose (DMEM-H) containing 0 μmol/L Pro, 400 μmol/L Pro, or 400 μmol/L Pro + 10 mmol/L DFMO for 4 d. The results showed that maternal dietary supplementation with 1% Pro increased fetal weight; the protein and DNA concentrations of the fetal small intestine; and mRNA levels for potassium voltage-gated channel, shaker-related subfamily, member 1 (Kv1.1) in the fetal small and large intestines (P < 0.05). Supplementing Pro to either gilts or IPEC-J2 cells increased ODC protein abundances and polyamine concentrations in the fetal intestines and IPEC-J2 cells (P < 0.05). In comparison with the Pro group, the combined administration of Pro and DFMO reduced the expression of ODC protein and spermine concentration in the fetal intestine, as well as the concentrations of putrescine, spermidine and spermine in IPEC-J2 cells (P < 0.05). Meanwhile, the percentage of cells in the S-phase and the mRNA levels of proto-oncogenes c-fos and c-myc were increased in response to Pro supplementation, whereas depletion of cellular polyamines with DFMO increased tumor protein p53 (p53) mRNA levels (P < 0.05). Taken together, dietary supplementation with Pro improved fetal pig growth and intestinal epithelial cell proliferation via enhancing polyamine synthesis.  相似文献   

云南菜豌豆栽培技术要点     

刘玥  秦艳梅  杨萍 《现代农业科技》2021,15(15)

菜豌豆是云南省的重要经济作物。云南地理优势独特,气候优势突出,独有的土壤,使得菜豌豆肉质绵滑,鲜嫩爽口,营养丰富,深得人们的喜爱。随着云南省农业栽培技术的不断提高,菜豌豆种植面积和规模不断扩大,种植人数也不断增加。菜豌豆远销国内各地,促进农民增收和当地农民就业。根据资料调查与分析,总结出适合云南省菜豌豆栽培的技术要点,为提高菜豌豆品质与农民增产增收提高重要保障。  相似文献   

工业级棉短绒清理回收工艺的改进     

王如南  张军  霍永峰  袁新英  韩文杰 《中国棉花》2021,48(8):26-28

为降低棉短绒中的杂质含量，提高棉短绒的质量及收率，对棉短绒清理回收的生产工艺进行了改进，创新连续化多级除杂装备和短绒精细化分离工艺。棉籽清理除杂工艺中，增加了圆筛、棒条机，并且调整了风力清籽机组配置；短绒分离工艺中，增加了五联筛和新型棒条机。改进后的工艺能够提升对毛棉籽的除杂效果，彻底清理剥绒得到的棉短绒中的碎棉仁、棉壳及棉秆等杂质。此外，该工艺还能回收棉短绒下脚料，满足大型生产线的生产要求。  相似文献   

EPS364, a Novel Deep-Sea Bacterial Exopolysaccharide,Inhibits Liver Cancer Cell Growth and Adhesion     

Yun Wang  Ge Liu  Rui Liu  Maosheng Wei  Jinxiang Zhang  Chaomin Sun 《Marine drugs》2021,19(3)

The prognosis of liver cancer was inferior among tumors. New medicine treatments are urgently needed. In this study, a novel exopolysaccharide EPS364 was purified from Vibrio alginolyticus 364, which was isolated from a deep-sea cold seep of the South China Sea. Further research showed that EPS364 consisted of mannose, glucosamine, gluconic acid, galactosamine and arabinose with a molar ratio of 5:9:3.4:0.5:0.8. The relative molecular weight of EPS364 was 14.8 kDa. Our results further revealed that EPS364 was a β-linked and phosphorylated polysaccharide. Notably, EPS364 exhibited a significant antitumor activity, with inducing apoptosis, dissipation of the mitochondrial membrane potential (MMP) and generation of reactive oxygen species (ROS) in Huh7.5 liver cancer cells. Proteomic and quantitative real-time PCR analyses indicated that EPS364 inhibited cancer cell growth and adhesion via targeting the FGF19-FGFR4 signaling pathway. These findings suggest that EPS364 is a promising antitumor agent for pharmacotherapy.  相似文献   

工程设施技术在稻渔共作模式中的发展          下载免费PDF全文

袁虹  刘萍 《农业工程》2020,10(6):41-45

稻渔共作模式是一种继承传统农业中遵循自然协调、绿色生态的农业发展理念并以现代农业技术为指导的生态循环农业模式，该模式具有稳粮、促渔、增收、提质、环境友好和可持续发展等多种生态系统功能，但缺乏稻田生态理论研究及新的生态管理技术，且基础设施不足，与种养技术匹配设施缺乏。工程设施技术属于稻渔共作模式配套技术，决定稻渔共作模式的发展潜力。目前工程设备设施在稻渔共作模式中的应用普及程度低，尚未开发出与稻渔共作模式特点相匹配的专用设备。结合稻渔共作模式的发展历史和水平，对配套工程设施技术的发展进行分析。   相似文献   

高产、抗病转基因抗虫棉品种——中棉所94A915     

范术丽  王龙  庞朝友  魏恒玲  王寒涛  王晖  喻树迅 《中国棉花》2017,44(1):35-35

概述了中棉所94A915的选育经过、特征特性，并总结了其关键栽培技术。  相似文献   

Alessandro Bonanno and Lawrence Busch (eds): Handbook of the international political economy of agriculture and food     

Marie Louise Ryan 《Agriculture and Human Values》2018,35(3):731-732

  相似文献   

新媒体时代社会主义核心价值观群众认同的路径探析     

张苹 《吉林农业科技学院学报》2017,26(2)

随着网络信息技术的发展,手机、平板电脑、数字电视等新媒体产品在人民群众中得到了广泛的应用,从而使得信息传播的互动性增强,出现了去中心化的趋势,为社会主义核心价值观的群众认同路径提供了机遇和挑战.如何利用新媒体时代创新社会主义核心价值观的传播载体,丰富传播内容、净化传播环境对于增强社会主义核心价值观的群众认同具有重要意义.  相似文献   

体细胞克隆荷斯坦奶牛出生后死亡犊牛内脏器官的组织病理学观察     

孙伟  包向男  乌云高娃  王建国  李永胜  李善铎  张铁柱  王飞飞  李喜和 《中国农学通报》2020,36(20):114-118

为探究体细胞克隆荷斯坦奶牛出生后死亡的原因,对新生后死亡的克隆荷斯坦公牛和自然繁殖的荷斯坦公犊的主要组织器官进行比较。通过解剖和石蜡切片-HE(hematoxylin-eosin staining)染色技术,对主要的组织器官结构进行观察和分析。结果表明,新生后死亡的克隆荷斯坦公牛肺部结构清晰;肝脏的肝细胞明显肿大,出现脂肪轻度变性;肾小管上皮细胞出现变性;心肌的肌纤维间空隙增大;骨骼肌纤维间隙明显,空泡变性,这可能导致该克隆牛犊肌肉无力并功能不全;淋巴结皮髓质界限不分明,淋巴小结细胞较稀疏,生发中心不明显,淋巴窦细胞较少;脾脏内红细胞较少,这说明该克隆牛的造血功能可能不完善;胸腺的皮质部分和髓质部分界限不明显,嗜酸性胸腺小体不易辨认,可能发育不全。该克隆公牛免疫器官出现不同程度的发育不全现象较严重,这有可能是其出生后死亡率高的主要原因。  相似文献   

进出境动物及动物产品风险分析     

王照修 《中国动物检疫》2003,20(7):11-11

加入WTO ,为我国经济发展提供了有利的发展机遇 ,但我国在对外贸易活动中却遭遇到技术性贸易壁垒 ,影响我国对外经济活动 ,使不少企业蒙受损失。为提高自身素质和了解国内外进出境动物及动物产品方面的知识 ,本人搜集了一些关于进出境动物及动物产品风险分析知识 ,现整理出来与同仁们共同学习。国际间动物和动物产品的贸易有很大的特殊性和复杂性。一方面 ,引进国外动物优良品种可以提高本国动物生产性能和质量 ,促进畜牧业生产发展和人民生活水平的提高 ;另一方面 ,动物的交换及动物产品的流通则有将国外动物传染病和寄生虫病传入的危险 …  相似文献