Effects of onion on serum uric acid levels and hepatic xanthine dehydrogenase/xanthine oxidase activities in hyperuricemic rats     

Haidari F  Rashidi MR  Keshavarz SA  Mahboob SA  Eshraghian MR  Shahi MM 《Pakistan journal of biological sciences: PJBS》2008,11(14):1779-1784

The aim of this study was to investigate the effects of onion on serum uric acid levels and hepatic Xanthine Dehydrogenase/Xanthine Oxidase activities in normal and hyperuricemic rats. Hyperuricemia was induced by intraperitoneal injection of 250 mg kg(-1) potassium oxonate in rats. Oral administration of onion at 3.5 and 7.0 mg kg(-1) day(-1) for 7 days was able to reduce serum uric acid levels in hyperuricemic rats with no significant effects on the level of this compound in the normal animals. In addition, onion when tested in vivo on rat liver homogeneities elicited significant inhibitory actions on the Xanthine Dehydrogenase (XDH) and Xanthine Oxidase (XO) activities. This effect resulted less potent than that of allopurinol. However, the hypouricemic effect observed in the experimental animal did not seem to parallel the change in XDH and XO activities, implying that the onion might be acting via other mechanisms apart from simple inhibition of enzyme activities. Such hypouricemic action and enzyme inhibitory activity of onion makes it a possible alternative for allopurinol, or at least in combination therapy to minimize the side-effects of allopurinol, in particular in long-term application.  相似文献   

Effect of hydrothermaled bran on physicochemical, rheological and microstructural characteristics of Sangak bread   总被引：1，自引：0，他引：1  

Laleh Mosharraf  Mahdi Kadivar  Mohammad Shahedi 《Journal of Cereal Science》2009,49(3):398-404

Increased consumption of whole grain products has been associated with decreased risk of health problems such as cardiovascular disease, diabetes and obesity. Phytate however, has always been a matter of concern, since it chelates minerals such as iron. Sangak is very popular Iranian flat bread invented five hundred years ago, made from 95% extraction flour. The bread is fermented but usually fermentation is not able to remove all phytate from the dough. In this study flours were first debranned; resulting bran was subjected to a hydrothermal process. The hydrothermaled (HT) brans were then incorporated in the Sangak flour. In the next step, physicochemical, rheological and microstructural characteristics of Sangak flour and dough prepared from two Iranian wheat varieties, Tajan and Back Cross of Roshan were investigated. Results indicated a reduction in phytate up to 55% in the samples. The resulting dough containing HT bran showed a higher development time and valorimetric value and was more stable than doughs made with normal bran. Dough made with HT bran showed a kind of protein matrix in which proteins and starch granules are oriented in a more non-ordered structure.  相似文献   

Silver nitrate and adenine sulphate induced high regeneration frequency in the recalcitrant plant Cosmos bipinnatus using cotyledon explants     

Mahdi Jaberi  Behzad Gharehyazi  Mahmood Khosrowchahli  Ali Sharafi  Nafiseh Aboofazeli 《The Journal of Horticultural Science and Biotechnology》2018,93(2):204-208

Plant regeneration ability was studied in the medicinal-ornamental plant, Cosmos bipinnatus ‘Sonata white’, which is a dicotyledonous recalcitrant plant to shoot induction. Cotyledons were used as sources of explants to investigate plant regeneration. High frequency of direct shoot induction was obtained when BA (5 mg/l) and AgNO₃ (5 mg/l) were used in combination with 20 mg/l adenine sulphate (73.8%) in Murashige and Skoog (MS) medium. The highest shoot number per explant (5.7) was induced on MS medium supplemented with 5 mg/l BA, 5 mg/l AgNO₃, and 40 mg/l adenine. Eight week-old shoots were transferred to root induction media containing MS and half-strength MS medium with different concentration of IBA. The highest rate of root induction (70.8%) was obtained on half-strength MS medium with 1.5 mg/l IBA within four weeks. The plantlets were transferred to pot and kept in the greenhouse condition. Seventy percent of the plantlets successfully acclimatised.

Abbreviations: BA, 6-benzylaminopurine; IBA, Indole-3-butyric acid; MS, Murashige and Skoog; PGRs, plant growth regulators.  相似文献   

Effects of blend of canola oil and palm oil on nutrient intake and digestibility,growth performance,rumen fermentation and fatty acids in goats          下载免费PDF全文

Kazeem Dauda Adeyemi  Awis Qurni Sazili  Mahdi Ebrahimi  Anjas Asmara Samsudin  Abd Rasak Alimon  Roselina Karim  Saiful Anuar Karsani  Azad Behnan Sabow 《Animal Science Journal》2016,87(9):1137-1147

The study examined the effects of blend of 80% canola oil and 20% palm oil (BCPO) on nutrient intake and digestibility, growth performance, rumen fermentation and fatty acids (FA) in goats. Twenty‐four Boer bucks were randomly assigned to diets containing 0, 4 and 8% BCPO on a dry matter basis, fed for 100 days and slaughtered. Diet did not affect feed efficiency, growth performance, intake and digestibility of all nutrients except ether extract. Intakes and digestibilities of ether extract, unsaturated fatty acids (FA) and total FA were higher (P < 0.05) while digestibility of C18:0 was lower (P < 0.05) in oil‐fed goats than the control goats. Total volatile FA, acetate, butyrate, acetate/propionate ratio and methane decreased (P < 0.05) with increasing BCPO but propionate, NH₃‐N and rumen pH did not differ between diets. Ruminal concentration of C18:0, n‐3 FA and total FA increased (P < 0.05) while C12:0, C14:0, C15:0 and n‐6 FA decreased with increasing BCPO. Analysis of the FA composition of Triceps brachii muscle showed that concentrations of C16:0, C14:0 and C18:2n‐6 were lower (P < 0.05) while C18:1n‐9, C18:3n‐3 and C20:5n‐3 were higher in oil‐fed goats compared with control goats. Dietary BCPO altered muscle lipids without having detrimental effects on nutrient intake and digestibility and growth performance in goats.  相似文献   

Cloning and expression of coxsakievirus B3 viral protein-1 in E. coli     

Maghsoudi N  Khalilpour A  Kamali M  Zeinoddini M 《Iranian Biomedical Journal》2007,11(3):147-152

BACKGROUND: Viral protein-1 (VP1) is a major capsid protein of Coxsakievirus B3 (CVB3) that plays an important role in directing viruses towards permissive cells and acts as a main antigenic site of the virus in eliciting of host immune response, hence it seems VP1 can be considered as a vaccine candidate against CVB3 infection. In this study, cDNA of VP1 was prepared, cloned into pET expression vector and the recombinant protein (VP1) was over expressed in E. coli. METHODS: The viruses were grown in suspension cultures of Vero cells with an input virus multiplicity of 10-50 plaque-forming units/cell. After observing complete cytopathic effect, the total RNA (cells and virus) was prepared for RT-PCR and by using specific primers, VP1 cDNA was amplified and ligated into pET vectors (32 a and 28 a). The recombinant vector was transferred into competent E. coli (BL-21) and after selection of proper colony, which carried correct cDNA within the vector; cells were cultured and induced with isopropyl B-D-thiogalactopyranoside, in order to express protein (VP1). The cultures were tested for presence of VP1 by SDS-PAGE and Western-Blotting analysis. RESULTS: Molecular techniques such as PCR which showed exact defined size of the VP1 (819 bp), restriction digestion and finally immunoblot analysis of over expressed protein; all confirmed the correct cloning and expression of VP1 in this research. CONCLUSION: In this research, full length of VP1 as major capsid protein of CVB3 was over expressed in E. coli which, can be used for further studies, including neutralizing antibody production against CVB3.  相似文献   

Application of bio-desalinization for reclamation of salt-affected soil under composted cow manure and deficit irrigation with saline water     

Kalanaki  Mahdi  Ritzema  Henk  Bamshad  Roghayeh  Jones  Edward  Fazilatnia  Mojtaba 《Paddy and Water Environment》2020,18(2):469-479

Paddy and Water Environment - Application of common leaching and flushing methods is often impractical due to freshwater scarcity, groundwater pollution and a lack of suitable drainage systems. To...  相似文献   

Response of selected indigenous dryland agroforestry tree species to salinity and implications for soil fertility management     

Hadia Seid  Emiru Birhane  Fassil Kebede  Kiros Meles Hadgu  Aklilu Negussie  Lindsey Norgrove 《Agroforestry Systems》2016,90(6):1133-1142

  相似文献   

Effect of vermicompost on some morphological,physiological and biochemical traits of bean (Phaseolus vulgaris L.) under salinity stress     

Abdollah Beykkhormizi  Parvaneh Abrishamchi  Ali Ganjeali  Mahdi Parsa 《Journal of plant nutrition》2016,39(6):883-893

Vermicompost can play an effective role in plant growth and development and also in reducing harmful effects of various environmental stresses on plants due to its porous structure, high water storage capacity, having hormone-like substances and plant growth regulators and also high levels of macro and micro nutrients. The aim of this study was to investigate the effects of vermicompost and salinity interactions on some morphological and physiological features and concentration of mineral elements of bean (Phaseolus vulgaris L. cv. Light Red Kidney) cultivar. A factorial experimental with five different volumetric ratios of vermicompost and sand, including to: 0:100; 10:90; 25:75; 50:50 and 75:25 and four levels of salinity [20, 40, 60 and 80 mmol l^?1 sodium chloride (NaCl)], and control was conducted base on Randomized Complete Block Design with three replications. Bean seeds were sowed in plastic pots, the seedlings being sampled 42 days old (flowering stage).The results showed that vermicompost had significant effect on all studied traits under stress and non-stress (p ≤ 0.05).In this experiment, the vermicompost significantly increased the photosynthetic rate and concentrations of potassium (K⁺) and calcium (Ca²)⁺in leaf and root tissues. In salinity levels of 20, 40 and 60 mmol l^?1NaCl, all subjected ratios of vermicompost and in 80 mmol l^?1NaCl the ratios of 10% and 75% vermicompost, significantly ameliorated negative effects of salinity. In both stress and non-stress conditions, using 10% volume of vermicompost is recommended to improve the growth of bean plants.  相似文献   

Soil and water conservation in ethiopia     

Mahdi Osman  Petra Sauerborn 《Journal of Soils and Sediments》2001,1(2):117-123

The article shows results of an international and interdisciplinary project with the title ‘Rainfall and its Erosivity in Ethiopia’. The article contains information about the traditional soil conservation practices, the reasons for intervention with traditional soil conservation technologies, the historical background of instutionalized soil conservation activities, the institutional frame work for soil conservation activities and their achievements, and the different departments and organisations for soil and water conservation as far as soil erosion in Ethiopia is concerned. As a conclusion and recommendation, the major constraints of the past activities and the current situation including future trends were discussed.  相似文献   

Potassium isotherm partitioning based on modified quantity‐intensity relation and potassium buffering characterization of soils of North India     

Shabir A. Bangroo  Nayar A. Kirmani  Mohammad A. Bhat  Javaid A. Wani  Asif M. Iqbal  Zahoor A. Dar  Syed Sheraz Mahdi  Ajaz A. Malik 《植物养料与土壤学杂志》2021,184(1):112-122

Background : Potassium (K) availability in soil and plant uptake is restrained by the dynamic interactions among the different pools of K. Aims : To understand these interactions, a study was undertaken to assess the quantity–intensity (Q/I) and buffering characteristics of rainfed maize (Zea mays L.) growing soils. Ten contrasting soils were evaluated for K partitioning changes in exchangeable K (ΔEK) and non‐exchangeable K (ΔNEK) pools in the soil‐solution phase and buffering characteristics using a modified version of Q/I approach. Results : The partitioned Q/I isotherms showed strong adsorption with the increase in K concentration ratio (CR^K) and the changes due to ΔEK were higher than changes due to ΔNEK. Total buffering capacity (PBC^K) significantly correlated (r = 0.92, p <0.01) with clay content with a major share contributed by buffering capacity owing to non‐exchangeable K ( ${\mathrm{PBC}}_{\Delta \mathrm{NEK}}^K$) rather than exchangeable K ( ${\mathrm{PBC}}_{\Delta \mathrm{EK}}^K$). The fixation capacity (β) factor, the magnitude of added K converted into a non‐exchangeable pool, ranged from 41 to 63%, whereas release (α) factor, the magnitude of added K converted to the exchangeable pool, ranged from 19 to 36%. Both threshold solution K (CK_r) and threshold exchangeable K (EK_r) values were found to be high in Satran clay loam (S2) and lower in Doon silty clay loam (S3) soils. The equilibrium exchangeable K (EK_o) was found close to minimum exchangeable K (E_min) in Doon silty clay loam (S3) and Babaweyl sandy clay loam (S1) soils and overall E_min constituted about 8.94 to 0.57% of the EK_o. Conclusion : It may be concluded that K Q/I isotherm partitioning provides a valuable insight to assess the dynamic relations. The ratio of α/β (K recharge index) could be used to evaluate the K enrichment capacity of soil to K additions while EK_r and E_min can be potentially useful in the elucidation of exchangeable K as K fertility index especially in soils with poor K fertilizer management.  相似文献