红茶色素形成机理的研究   总被引：14，自引：2，他引：12  

夏涛 《茶叶科学》1999,19(2):139-144

在茶鲜叶匀浆悬浮发酵体系中, 加入铜酶抑制剂NaDDC可使茶黄素(TFs) 的总量下降,而其各组分的消长趋势则各异, 然而对茶红素(TRs) 的形成似乎并无明显的影响( 高浓度的NaDDC除外) , 表明TRs 的形成存在多条平行途径; 在发酵体系中加入不同比例蒸青叶( 酶已钝化) 的试验表明, 对TFs 和TRs 的形成量未产生影响, 说明酶浓度不是红茶发酵的限制因子; 降低发酵体系的pH (pH=4-0) , 虽然使多酚氧化酶(PPO) 和过氧化物酶(POD) 的稳定性明显下降, 且PPO活性降幅大于POD, 但由于减缓了TFs 的非酶性氧化消耗速率, 因而TFs 的含量显著增加。  相似文献   

云南茶树资源遗传多样性与亲缘关系的ISSR分析   总被引：8，自引：1，他引：8  

刘本英  李友勇  唐一春  王丽鸳  成浩  王平盛 《作物学报》2010,36(3):391-400

以8个种群134份云南茶树资源为材料,应用ISSR标记方法,进行了遗传多样性与亲缘关系分析。结果表明,18个多态性ISSR引物对全部试验材料进行PCR扩增,共获得475条稳定的谱带,其中多态性谱带470条(占98.9%),遗传多样性丰富;应用Nei-Li相似系数法估算了134个材料间的相似系数为0.445~0.819,平均为0.512,说明茶树资源间的遗传基础较宽;对134份茶树资源的分子系统聚类分析(UPGMA)将资源分为3大组,聚类结果与地理距离没有明显的相关性;主成分分析(PCA)表明主成分分析的结果与系统聚类基本一致,但是主成分分析更加直观清晰地显示各个材料间的亲缘关系;大厂茶等8个种群间的遗传相似系数于0.850~0.987间,平均为0.92,表明不同种群间的遗传差异较小。  相似文献   

利用ISSR标记分析福云（半）同胞系茶树品种（系）的遗传多样性和亲缘关系     

余继忠  杨亚军  黄海涛  陈亮  姚明哲 《广西农业生物科学》2009,(2):281-288

福鼎大白茶和云南大叶茶是茶树育种中的骨干亲本,利用这两个亲本选育了许多优良的品种（系）。本研究利用ISSR标记技术分析了40个福云（半）同胞系茶树品种（系）的遗传变异水平和亲缘关系。14个ISSR引物在供试品种（系）间共扩增出251条谱带,多态性条带的比率为96.4％。引物的PIC值平均为0.94,Rp值平均为29.35,表明引物扩增位点的高多态性和对品种的强辨别能力。40份供试品种（系）的基因多样性指数（H）为0.35,Shannon信息指数（I）为0.52。按母本来源和育种机构对供试品种（系）进行分组分析,结果表明不同品种（系）组间的遗传多样性水平比较接近,遗传变异主要存在于组内品种（系）的个体之间,不同组间基因交流明显。供试品种（系）间的相似系数介于0.52-0.75,根据相似系数矩阵按UPGMA法对40个供试品种（系）进行聚类分析,构建了不同品种（系）间的亲缘关系树状图。福鼎大白茶在树状图中形成单独的分支,在树状图的根基处,其它品种（系）根据遗传距离聚类成不同的类群。来源于同一育种单位的部分茶树品种（系）聚类在同一类群中,但未发现按母本来源区分的独立类群。总之,通过ISSR标记分析,可在基因组水平上进一步了解福云（半）同胞系茶树品种（系）间的遗传变异水平,并进一步明确其亲缘关系,为今后福云（半）同胞系在茶树育种上的有效利用提供依据。  相似文献   

The Effect of Intravalvular Liquid Loss on Changes in Escherichia coli Levels in Live,Refrigerated Mussels (Mytilus galloprovincialis)     

Slaven Jozić  Katarina Milanković  Tea Brajčić  Mladen Šolić  Danijela Šantić  Marin Ordulj 《Journal Of Aquatic Food Product Technology》2017,26(4):491-500

We investigated the effect of intravalvular liquid loss on changes in Escherichia coli (E. coli) levels in mussels (Mytilus galloprovincialis) harvested in different seasons and refrigerated (5°C) for 72 hours. A positive effect of seawater temperature at the time of harvesting on intravalvular liquid loss was found. No changes in E. coli levels in mussels (flesh and intravalvular liquid), as the result of intravalvular liquid loss, were observed. Levels of E. coli in the flesh decreased, probably as a result of cells dying off, causing the maintenance of E. coli levels in flesh and intravalvular liquid to be stable throughout storage.  相似文献   

Phenolic composition and antioxidant activity of aqueous infusions from Capparis spinosa L. and Crithmum maritimum L. before and after submission to a two-step in vitro digestion model     

Siracusa L  Kulisic-Bilusic T  Politeo O  Krause I  Dejanovic B  Ruberto G 《Journal of agricultural and food chemistry》2011,59(23):12453-12459

This study investigated the phenolic composition and antioxidant activities of aqueous infusions from wild-grown caper (Capparis spinosa L.) and sea fennel (Crithmum maritimum L.) from the Dalmatia region (Croatia) before and after their submission to an in vitro digestion process. HPLC/UV-vis-DAD/ESI-MS analysis of the caper infusion identified rutin, kaempferol 3-O-rutinoside, and isorhamnetin 3-O-rutinoside as dominant flavonoids in the matrix together with a series of cinnamoylquinic acid derivatives, whereas in the sea fennel aqueous infusion chlorogenic acid (5-caffeoylquinic acid), its isomers, and higher derivatives were identified as almost the sole class of phenolics. Both infusions exhibited good and dose-dependent antioxidant activity before in vitro digestion by the DPPH method, the β-carotene bleaching method, and copper-induced oxidation of human LDL. The amount of total phenolics (Folin-Ciocalteu assay) strongly decreased in digested samples (from 3.0 and 2.2% in caper and sea fennel infusions, respectively, to <1.0%), as did their antioxidant activity as measured by the three aforesaid methods. The results showed that the majority of phenolic compounds detected in both infusions are not stable under applied simulated gastrointestinal conditions and that the stability of these secondary metabolites strongly depends on the nature of the corresponding matrix.  相似文献   

2009年度浙江茶业发展报告     

浙江省茶叶产业协会 《中国茶叶》2010,32(4)

2009年,浙江茶产业经受住了国际金融危机和经济衰退的冲击.继续保持较快发展的势头,取得了茶叶增产、茶农增收、市场购销两旺、出口量值稳定的可喜成绩.  相似文献   

不同品种茶园害虫及天敌群落多样性研究   总被引：1，自引：1，他引：1  

彭萍  王晓庆  李品武  雷蕾  王之劲  林强  黄尚俊 《西南大学学报(自然科学版)》2011,33(6)

通过对不同品种茶园(巴渝特早、福鼎大白茶、川茶群体种)害虫群落结构及天敌种类进行一年的系统调查,结果表明:茶园中害虫及天敌共有2纲,20目,41科;其优势种群基本相似,主要是同翅目和蜘蛛目;川茶群体种茶园的物种丰富度指数、多样性指数、均匀度指数均高于其他两个品种茶园,且3个品种茶园的害虫和天敌种类具有高度相似性.  相似文献   

Functional Components of Tea （Camellia Sinensis） Germplasms Collected in Korea     

Min-Seuk Lee  Joo Yoo  Jin-Ho Lee  Kwan Jeong Song 《茶叶》2013,(4):199-200

This study was carried out to obtain useful information and to search quality related components for breeding of tea by analysis of Korean local tea leaves at Sulloc-Cha R＆D center in Jeju Island,Korea.It was also conducted to measure the major agronomic characteristics of 300 local germplasms.These results allowed us to select some superior lines as genetic resources for green-tea breeding and research genotypes.  相似文献   

The Beauty of Elegance ‘Pungnyu＇ in the Tea Culture of Korea     

YS Joung 《茶叶》2013,(4):524-527

‘Tea culture＇ broadly includes ‘providing tea＇,‘ the tea ceremony＇,‘ tea ritual＇,‘ tea artistry＇ and ‘ tea party＇.The concept of ‘Pungnyu＇ in Korea was proposed by Choi Chi-won （857 ～ 894？）,who pointed out that ＂there is a profound truth ‘ Tao＇ in Korea,which was considered to be ‘ Pungnyu＇ or the beauty of elegance.The origin of ‘Pungnyu＇ can be traced back to the history of hermits.‘Pungnyu＇ includes the teachings of Buddhism,Confucianism and Taoism.  相似文献   

茶树黄酮醇合成酶基因的克隆与原核表达     

马春雷  陈亮 《广西农业生物科学》2009,(3):433-438

本研究采用EST测序技术和RT—PCR技术,获得了一个茶树茶多酚代谢中的重要基因——黄酮醇合成酶（FLS）基因,在GenBank登录（GenBank accession No．EF205150）,其序列全长1317bp,其中开放阅读框长996bp,编码331个氨基酸,3]端有一个明显的多聚腺苷酸加尾信号,推测的蛋白分子量约为37．5kD,理论等电点为5．80。序列分析表明它与葡萄FLS基因序列的亲缘关系比较近。将该基因重组到表达载体pET-32a（＋）中进行原核表达,经IPTG诱导、SDS-PAGE检测,结果表明茶树黄酮醇合成酶基因能在大肠杆菌BL21中表达,电泳检测到一条大约61kD的外源蛋白,与预测的融合蛋白分子量相符。用Ni-NTA亲和层析柱对融合蛋白进行纯化,得到了纯度在90%以上的纯化蛋白,为进一步研究PET-FLS融合蛋白的活性及功能奠定了基础。  相似文献