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Kampen AH Tollersrud T Larsen S Roth JA Frank DE Lund A 《Veterinary immunology and immunopathology》2004,97(1-2):105-114
Five methods for measurement of phagocytosis and respiratory burst activity of bovine blood polymorphonuclear leukocytes (PMNs) were evaluated. Eight cows were repeatedly sampled over a two week period and parallel samples tested in all five assays to assess the repeatability and stability of the methods. In the flow cytometric phagocytosis assay, ingestion of fluorescein labeled bacteria was measured, and in the flow cytometric assay for respiratory burst, oxidation of a dye by reactive oxygen species was recorded. In the classical assays, bactericidal effect on opsonized, live bacteria was quantified by the conversion of an indicator substance, superoxide anion production was assayed by the reduction of cytochrome c, whereas myeloperoxidase activity was determined with a radioactive iodination assay. The results showed that the Phagotest, Bursttest, cytochrome c and iodination assays gave repeatable results when samples were run in the same setup on the same day. Although day-to-day variability was significant in all assays, the described methods comprise a panel of useful tests for the evaluation of phagocytosis and respiratory burst activity in bovine PMNs. The flow cytometric methods represent a convenient alternative to the classical methods for measurement of phagocytosis and respiratory burst in bovine blood PMNs. 相似文献
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S. J. Kleinschuster H. J. Rapp J. Bier K. R. van Kampen R. S. Spendlove A. V. Johnston Y. Elsner 《Veterinary research communications》1979,3(1):65-71
A method is reported for the continuous in vitro culture of epithelial cells derived from bovine ocular squamous cell carcinoma. 相似文献
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Ricardo A．Sooncini MVD 《国外畜牧学(猪与禽)》2012,32(7)
3预防沙门氏菌传播的措施
3.1卫生和消毒
在引进新一批鸡群前,所有鸡舍和器具必须进行清洁消毒,特别是在前一批鸡群为沙门氏菌阳性的情况下,清洁消毒就更有必要.卫生措施的有效性可通过严格的细菌学定量检测验证,要求肠道细菌计数小于103个/25 m2. 相似文献
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Experimental validation of geosmin uptake in rainbow trout,Oncorhynchus mykiss (Waldbaum) suggests biotransformation 
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下载免费PDF全文 Edward Schram Johan W Schrama Tobias van Kooten Christiaan J A F Kwadijk Harm Kampen Jan W van de Heul Johan A J Verreth Albertinka J Murk 《Aquaculture Research》2018,49(2):668-675
The bioconcentration of waterborne geosmin in rainbow trout, Oncorhynchus mykiss (Waldbaum) was assessed. Fifty rainbow trout with a mean (SD) weight of 226.6 (29.0) g and lipid content of 6.2 (0.6) % (w/w) were exposed to geosmin in static water for 0, 2, 4, 6, 8, 12, 24, 36, 48 and 120 hr, with one tank containing five fish for each exposure period. Geosmin concentrations were measured in fish tissue and water samples collected over time. With time the geosmin concentration in the fish increased and decreased in the water. However, the total absolute amount of geosmin in the system declined over time which could be explained by induction of biotransformation. This is in accordance with the decreasing lipid normalized geosmin levels in the liver compared with the liver‐free carcass. Geosmin distribution within rainbow trout clearly is not exclusively governed by the lipid content of tissues. In vivo geosmin bioconcentration in rainbow trout is slower and the body burden reached is lower than the generally accepted theoretical model predicts. 相似文献
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Effect of prednisolone treatment on selected respiratory parameters and cardiac output in prematurely delivered neonatal lambs. 下载免费PDF全文
下载免费PDF全文 Lambs were delivered by hysterotomy on days 142 and 143 postcoitum and were assigned to either a control group or a prednisolone-treated group (10 mg/kg daily for four days). On days 1 through 3 of life erythrocyte 2,3-diphosphoglycerate values were significantly increased in the treated group. Prednisolone treatment did not result in significantly increased P50 values. Mean plasma corticoid values in the control group decreased gradually postpartum. The prednisolone-treated lambs compared to the control lambs had depressed corticoid values, differing significantly on day 4. Body weight gains were significantly depressed by treatment and this effect lasted beyond the treatment period. On day 1 of life (24 hours postinitial treatment) oxygen consumption and cardiac output were significantly elevated in the treated group. 相似文献
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1. The energetic efficiency of growing chickens given daily injections of corticosterone was investigated by measuring metabolic activity and energy balance. 2. Water consumption increased significantly within the first day of treatment ; food consumption increased by the third day of treatment. 3. Growth rate, which was suppressed during the first two days, returned to normal by the third day of treatment. 4. Excreted water, dry matter and uric acid increased within the first day of treatment. 5. Heat production was not changed by treatment; however, the CO2:O2 respiratory ratio (RQ) was increased by the third day and exceeded 1·0 on days 4 and 5 of treatment. 6. Leg muscle weight was decreased and abdominal fat and liver weight were increased by corticosterone treatment. 7. Balance data indicated that energy absorption efficiency was decreased and energy retention efficiency was increased by corticosterone treatment, confirming the RQ, and carcase findings that greater amounts of energy were stored as fat. 相似文献