Study on Synergistic Effect of VA5 Immunopotentiator on Pigeon NDV Inactivated Vaccine     

XIONG Xiaoyan  QIN Zhenbin  MO Hongfang  HE Dongxian  YU Lintian  XU Jiarong 《中国畜牧兽医》2007,47(12):4032-4040

This study was designed to evaluate the synergistic effect of VA5 immunopotentiator on pigeon ND4416 inactivated vaccine.160 healthy pigeons were randomly divided into four groups,including ND4416 strain inactivated vaccine group (NDV group),ND4416 strain inactivated vaccine and immunopotentiator (VA5) mixed group (NDV+VA5 group),La Sota inactivated vaccine group (La Sota group) and normal saline as the control group (C group),to assess their vaccine efficacy against virulent pigeon NDV by serological analysis and animal testing.Pigeons sera were collected at different time points after immunization and measured the HI antibody titer of each group.The results showed that VA5 immunopotentiator significantly improved the serum antibody level of pigeons immunized with pigeon Newcastle disease inactivated vaccine (P<0.05).In addition,comparative test of spleen lymphocyte transformation was conducted at various time points after immunization.The results indicated that VA5 effectively stimulated the lymphocyte transformation of immune pigeon.Pigeons in each groups were challenged with ND4416 strain at the 30th,90th and 180th d after immunization.The results presented that the NDV+VA5 group had 100% protection rate and higher than La Sota group.The duration of immunization test showed that the antibody titer of NDV+VA5 group reached peak 11.20log₂ at the 21st d,remained 7.50log₂ at 180th d,and the protection rate remained 100% at 180th d.It indicated that VA5 immunopotentiator sustained the immune duration of pigeon NDV vaccine up to 180 d.Moreover,the in vitro detoxification test results suggested that VA5 immunopotentiator reduced the in vitro detoxification cycle of pigeons after challenge.Overall,this study suggested VA5 immunopotentiator could significantly improve the immune efficacy of pigeon Newcastle disease inactivated vaccine,which provided a basis for further enhancing the efficacy of Newcastle disease vaccine,as well increased experimental data for the application of immunopotentiators.  相似文献   

猪肺炎支原体 MY-99株的致病性   总被引：1，自引：0，他引：1  

熊焰  石谦  邓均华  苟琳 《中国兽医学报》2006,26(3):259-261

将猪肺炎支原体MY-99株人工培养F100株经鼻腔接种于健康成华×大约克杂交猪,2周后60%的猪出现气喘,个别猪伴有轻微咳嗽;病理切片可见到支气管周围多量淋巴样细胞浸润、肺泡壁增厚等典型的间质性肺炎病变.试验猪平均日增重比对照猪显著降低,接种后0～14 d期间降低了31.05%(P<0.01),15～28 d期间降低了42.01%(P<0.01),29～42 d期间降低了0.1%(P>0.05).试验猪血清中的猪肺炎支原体抗体水平在2周后最高(1∶24.2),4周后降至1∶23.9,6周后最低(1∶22.5),显示猪肺炎支原体MY-99株诱发了急性猪地方流行性肺炎,同时从试验猪肺脏中分离出了猪肺炎支原体.超微切片显示,其菌体大小为0.33～0.48 μm,并缺乏细胞壁;革兰氏染色为阴性类球形菌体,在压力下可以通过0.45 μm的细菌滤膜,菌落呈现煎蛋状.  相似文献   

钾对猕猴桃果实品质与贮藏的影响   总被引：13，自引：1，他引：13  

王仁才  夏利红  熊兴耀  李大志 《果树学报》2006,23(2):200-204

为提高猕猴桃果实品质与耐贮性,以7年生美味猕猴桃品种米良1号(Actinidiadeliciosacv.Miliang-1)为试材,进行果实生长发育期施钾处理对果实品质与贮藏性能影响的研究。结果表明:适量施钾能提高果实硬度及可溶性固形物与维生素C含量,降低果实含酸量,提高果实耐贮性。在0-4℃贮藏条件下,以株施纯K2O80g+叶面喷施0.3%硫酸钾处理果实贮藏效果最佳,贮藏73d时,其软果率最低(15.56%),果肉硬度最高(0.27MPa)。同时果实维生素C含量的下降趋势较为平缓,果实贮藏寿命延长。贮藏6个月时,株施纯K2O80g+叶面喷施0.3%硫酸钾处理的果实好果率(68.89%)明显高于对照果实(0)。  相似文献   

美系獭兔的胴体性状和肉品质特性研究   总被引：4，自引：0，他引：4  

熊国远  朱秀柏 《中国畜牧兽医》2007,34(5):69-74

通过对20只美系獭兔屠宰性能和肉品质的测定，研究獭兔的生产性能和肉质特性及它们之间的关系，结果表明：美系獭兔5月龄的平均体重为2.533 kg,商用皮重0.410 kg,屠宰率为60.70%，右后腿重0.182 kg，体长和腰周长分别为30.69和18.04 cm；美系獭兔背最长肌和后腿肉的pH1(宰后1 h的pH)分别为6.42和6.43，pH24(宰后24 h的pH)分别为6.22和6.35,肉色L*值分别为54.66和52.67，a*值分别为12.37和9.89，b*值分别为9.06和4.08；兔肉的滴水损失、熟肉率、蒸煮损失、剪切力值、水分含量和肌内脂肪含量分别为2.06%、62.12%、30.44%、2.453 kg.f、73.60%和1.67%。商用皮重与热胴体重、体长存在极显著的正相关（P<0.01），与商用胴体重存在显著的正相关（P<0.05）。兔的体重（热胴体、商用胴〖JP2〗体、右后腿）、体型（体长和腰周长）与滴水损失、蒸煮损失呈负相关，与熟肉率、剪切力值呈正相关，但相关性都不显著（P>0.05）。  相似文献   

Real-time PCR方法和PCR方法检测虾白斑综合征病毒     

熊炜  邱璐  李健  蒋静  王巧全  李树清  杨锡佺  胡永强 《中国预防兽医学报》2007,29(2):138-141

由白斑综合征病毒引起的虾白斑综合征是国际兽医局（OIE）规定的必须上报的水生动物二类疫病。本研究首先通过设计新的PCR引物，提高了PCR检测白斑综合征病毒的阳性检出率。为进一步提高白斑综合征病毒检测的灵敏度，本研究采用TaqMan探针技术建立了快速检测白斑综合征病毒的real-time PCR方法，通过与常规PCR方法比较，证实其检测灵敏度显著提高。  相似文献   

快速鉴别诊断山羊痘病毒和羊口疮病毒二联PCR方法的建立   总被引：2，自引：1，他引：2  

朱伟  熊毅  刘棋  郭建刚  李华明 《中国预防兽医学报》2007,29(5):394-396

参照GenBmk已发表的山羊痘病毒(GPV)和羊口疮病毒(ORF)的核苷酸序列设计了两对引物,建立了一种可以快速鉴别山羊痘病毒和羊口疮病毒二重PCR方法,检测结果显示,对山羊痘病毒和羊口疮病毒可以分别扩增出413 bp和595 bp的特异性片段,经敏感性测定,最低能检测到4 pg的DNA。对广西的送检的山羊病料20份进行检测,其中7份可扩增出413 bp的山羊痘特异片段、1份可扩增出595 bp的羊口疮特异片段。该方法能在4 h内完成整个检测过程,不仅快速,而且特异强、敏感性高,很适合于快速诊断。  相似文献   

Neuropeptide Y induced redistribution of intracellular free calcium in rat cardiomyocytes     

XIONG Long-gen  ZHANG Yi  SU Tao  LIU Shi-ming  DONG Qi  CHEN Min-sheng 《园艺学报》2007,23(7):1254-1257

AIM: To investigate the effect of neuropeptide Y (NPY) on intracellular free calcium(［Ca²⁺］_i) and Ca²⁺ sarcoplasmic reticulum of cardiomyocytes in rats.METHODS: Cardiomyocytes of neonatal Sprague-Dawley rats were incubated with NPY at concentration of 100 nmol/L for 24 h. Fluorescent indicator Fluo-4 AM was used to detect ［Ca²⁺］_i and Fluo-5N AM was used to detect Ca²⁺ in sarcoplasmic reticulum (SR). Calcium image was recorded by laser scanning confocal microscope. The SR Ca²⁺ load was estimated by caffeine-induced Ca2+ transient (CCT). RESULTS: 24 h after incubation with NPY, compared with control group, the concentration of ［Ca²⁺］_i was significantly elevated (P<0.05), and the concentration of free Ca2+ in SR (［Ca²⁺］_SR) was significantly decreased (P<0.05), and the peak of CCT was attenuated.CONCLUSION: Stimulation with NPY for 24 h causes redistribution of free calcium in rat cardiomyocytes, namely the elevation in ［Ca²⁺］_i and decline in ［Ca²⁺］_SR.  相似文献   

生长调节剂对黄绿蜜环菌菌丝生长的影响   总被引：1，自引：0，他引：1  

周劲松  熊辉岩  盛海彦  焦迎春  杨春江 《食用菌学报》2007,14(3):44-46

选用三十烷醇(triacontanol)和肌醇(inositol)两种生长调节剂对黄绿蜜环菌(Armillaria luteo-virens)菌丝生长的影响进行研究。结果表明:三十烷醇和肌醇对菌丝生长都有较明显的影响,但肌醇效果不如三十烷醇。在培养基中添加1.5ⅹ10-7mg.mL-1三十烷醇,可明显提高菌丝生长速度,缩短培养周期。  相似文献   

混合絮凝剂处理中纤板厂木片水洗废水的研究     

熊燕萍  顾心慧  高克文  梅雅茹  徐咏兰 《江西林业科技》2012,(1):56-58

对中密度纤维板厂木片水洗废水处理方案进行研究:混合絮凝剂的成分和最佳投放量经研究确定为15 g/m3聚合氯化铝和1 g/m3聚丙烯酰胺,对木片质量也不会有较大损害;确定最佳处理水量占总水量的比例为15%,既能使废水水质得到改善,而且处理成本为最小,达到资源回收利用的目的。  相似文献   

In silico cloning and characterization of a novel transcription factor VvERF2 from Vitis vinifera with bioinformatics tools     

LIU Hong-jin  GAO Feng  XIONG Ai-sheng  PENG Ri-he  LI Xian  CAI Bin  XUE Yong  FU Xiao-yan 《上海农业学报》2008,24(3)

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