全文获取类型
收费全文 | 410篇 |
免费 | 22篇 |
专业分类
林业 | 18篇 |
农学 | 11篇 |
27篇 | |
综合类 | 24篇 |
农作物 | 8篇 |
水产渔业 | 41篇 |
畜牧兽医 | 235篇 |
园艺 | 5篇 |
植物保护 | 63篇 |
出版年
2023年 | 2篇 |
2022年 | 7篇 |
2021年 | 19篇 |
2020年 | 13篇 |
2019年 | 9篇 |
2018年 | 13篇 |
2017年 | 10篇 |
2016年 | 17篇 |
2015年 | 24篇 |
2014年 | 20篇 |
2013年 | 34篇 |
2012年 | 34篇 |
2011年 | 28篇 |
2010年 | 14篇 |
2009年 | 11篇 |
2008年 | 25篇 |
2007年 | 21篇 |
2006年 | 21篇 |
2005年 | 26篇 |
2004年 | 23篇 |
2003年 | 17篇 |
2002年 | 12篇 |
2001年 | 6篇 |
2000年 | 4篇 |
1999年 | 3篇 |
1998年 | 6篇 |
1997年 | 1篇 |
1995年 | 1篇 |
1994年 | 1篇 |
1992年 | 1篇 |
1990年 | 1篇 |
1987年 | 1篇 |
1986年 | 2篇 |
1984年 | 1篇 |
1982年 | 1篇 |
1978年 | 2篇 |
1970年 | 1篇 |
排序方式: 共有432条查询结果,搜索用时 15 毫秒
81.
Chiaki Ikeda Yuki Manabe Nami Tomonaga Tatsuya Wada Takashi Maoka Tatsuya Sugawara 《Marine drugs》2020,18(12)
Halocynthiaxanthin is an acetylenic carotenoid mainly found in Halocynthia roretzi. To date, several bioactivities of halocynthiaxanthin have been reported, but its mechanism of digestion and absorption in mammals has not been studied yet. In this study, we evaluated the intestinal absorption of halocynthiaxanthin in mice. The halocynthiaxanthin-rich fraction was prepared from the tunicate Halocynthia roretzi. Mice were orally administered the fraction at a dose of 5 mg/kg body weight. The halocynthiaxanthin levels in the plasma, liver, and small intestine, were quantified using HPLC-PDA, 1, 3, 6, and 9 h after ingestion. The halocynthiaxanthin-rich fraction mainly consisted of the all-trans form and a small amount of cis forms. These three isomers were detected in the plasma of mice 3 h after ingestion. Time-course changes after the ingestion of this fraction were found, with cis isomers being more abundant than the all-trans isomer in the mouse plasma and liver. In the small intestine, however, the all-trans isomer was primarily detected. The possibility that cis isomers might be absorbed rapidly from the small intestine cannot be denied, but our results suggest that dietary all-trans-halocynthiaxanthin might be isomerized to the cis isomer after intestinal absorption. 相似文献
82.
Tomoya Tamura Yuki Okubo Yoshitaka Deguchi Shizu Koshikawa Masahiro Takahashi Yasushi Chida Keiji Okada 《Animal Science Journal》2019,90(4):589-596
Demand has been increasing recently for an automated monitoring system of animal behavior as a tool for the management of livestock animals. This study investigated the association between the behavior of dairy cattle and the acceleration data collected using three‐axis neck‐mounted accelerometers, as well as the feasibility of improving the precision of behavior classifications through machine learning. In total 38 Holstein dairy cows were used, and kept in four different farms. A logger was mounted to each collar to obtain acceleration data for calculating the activity level and variations. At the same time the behavior of the cattle was observed visually. Characteristic acceleration waves were recorded for eating, rumination, and lying, respectively; and the activity level and variations were significantly different among these behaviors (p < 0.01). Decision tree learning was performed on the data set from Farm A and validated its precision; which proved to be 99.2% in cross‐validation, and 100% in test data sets from Farms B to D. This study showed that highly precise classifications for eating, rumination, and lying is possible by using decision tree learning to calculate the activity level and variations of cattle based on the data obtained by three‐axis accelerometers mounted to a collar. 相似文献
83.
Fumiko TAGUCHI Rui TANAKA Sayuri KINOSHITA Yuki ICHINOSE Yoshiyuki IMURA Salamah ANDI Kazuhiro TOYODA Tomonori SHIRAISHI Tetsuji YAMADA 《Journal of General Plant Pathology》2001,67(2):116-123
To elucidate the role of harpins produced by Pseudomonas syringae, the corresponding hrpZ gene was isolated from P. s. pv. tabaci. The sequence information revealed that this gene carries a serious mutation with 326 bp lacking in the central region and
potentially encodes only 140 N-terminal amino acids because of a frame shift. The investigation of biological properties using
recombinant harpin indicated harpinpsta was incapable of inducing HR in both host and nonhost plants. Based on an immunoblot analysis to detect harpin from P. s. pathovars in hrp-inducing medium, the truncated harpinpsta was neither expressed nor secreted into the culture medium. These results suggest that harpin is not the sole determinant
of the host-parasite specificity in P. s. pv. tabaci.
Received 10 August 2000/ Accepted in revised form 21 December 2000 相似文献
84.
Mona Uchida Yuki Matsumiya Masaya Tsuboi Kazuyuki Uchida Takayuki Nakagawa Wataru Fujii Tetsuya Kobayashi Hajime Tsujimoto Aki Ohmi Hirotaka Tomiyasu Tomoki Motegi Shingo Maeda Yasuyuki Momoi Tomohiro Yonezawa 《Veterinary and comparative oncology》2023,21(3):391-400
Histiocytic sarcoma (HS) is a rare neoplasm of macrophages or dendritic cells with a poor prognosis in dogs. As the apoptosis inhibitor of macrophage (AIM) is characteristically expressed in canine macrophages, we hypothesised that AIM is involved in the development or progression of HS in dogs. In this study, AIM expression in the tumour region and serum AIM levels in dogs with HS was assessed. Additionally, the effects of AIM overexpression on HS cell viability were investigated using a HS cell line that was selected from five validated HS cell lines. Immunohistochemistry showed that AIM expression was observed in the cytoplasm of the HS cells. CD36, a candidate AIM receptor, was also observed on the cell membrane of HS cells. When the serum AIM level was detected in 36 dogs with HS and 10 healthy dogs via western blot analysis, the AIM levels in the HS dogs were significantly higher than those in the controls. AIM mRNA expression in the 5 HS cell lines varied but was higher than that in the other tumour-derived lines. Among the five HS cell lines, DH82 originally had lower AIM and the highest CD36 expression. When AIM was overexpressed in DH82, therein cell growth speed and invasion, apoptosis inhibition and phagocytic activity were strongly upregulated. These data suggest that elevated intra-tumour expression of AIM could induce the progression of HS cells in dogs. Moreover, elevated serum AIM levels in dogs with HS could serve as a biomarker of HS. 相似文献
85.
Terasawa N Saotome A Tachimura Y Mochizuki A Ono H Takenaka M Murata M 《Journal of agricultural and food chemistry》2007,55(10):4154-4159
Zuiki, a stalk of taro (Colocasia esculenta), is a traditional vegetable in Japan. Raw zuiki is often boiled and vinegared to eat. The surface color of zuiki is reddish. Here, we isolated a red pigment from zuiki and identified it as cyanidin 3-rutinoside using instrumental analyses. The color of zuiki disappeared by boiling, but the zuiki turned red again in an acetic acid solution. It seems that the cyanidin 3-rutinoside that exists on the surface of zuiki elutes in boiling water and then, the pigment that seeps out from the inside of the zuiki is exposed to an acid solution, and its surface turns red again. The radical scavenging activity of purified zuiki anthocyanin was 114 mg equivalent to BHT/g. About half of the anthocyanin in fresh zuiki was washed out by boiling, and the radical scavenging activity of zuiki was definitely reduced. 相似文献
86.
Yuki M Sugimoto N Takahashi K Otsuka H Nishii N Suzuki K Yamagami T Ito H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(4):397-399
A 9-year-old female Pug was presented to us with chronic diarrhea. Hematologic findings indicated severe hypoproteinemia and hypoalbuminemia, and endoscopy revealed severe edema of the duodenal mucosa. Based on these results and on additional histopathological findings, we made a diagnosis of protein-losing enteropathy caused by lymphocytic-plasmacytic enteritis with lymphangiectasia. The dog was initially treated with prednisolone and cyclosporine. This treatment regimen was not effective. However, when methotrexate was substituted for cyclosporine, progress was obtained and the diagnosis was confirmed. 相似文献
87.
Noto T Furuichi Y Ishiye M Matsuoka N Aramori I Mutoh S Yanagihara T Manabe N 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(8):803-807
Intravascular accumulation of blood cells after brain ischemia-reperfusion can cause obstruction of cerebral blood flow and tissue hypoxia/ischemia as a consequence. In the present study, we examined temporal and topographic changes of tissue hypoxia/ischemia after occlusion of the middle cerebral artery (MCA) for 60 min in rats with immunohistochemical staining for hypoxia (2-nitroimidazole hypoxia marker: hypoxyprobe-1 adducts). Our results showed that tissue hypoxia expressed as positive staining for hypoxyprobe-1 adducts preceded neuronal degeneration. Platelets and granulocytes were detected close to the hypoxyprobe-1 adducts positive area. These results suggested that the hypoxic environment could persist even after reperfusion of MCA, because of vascular obstruction with accumulation of platelets and granulocytes. 相似文献
88.
Yuki Tomonari Junko Sato Yumi Wako Minoru Tsuchitani 《Journal of toxicologic pathology》2012,25(4):287-291
To provide background data as the pathologic basis, the pineal glands of 190 male and 193
female Crl:CD(SD) rats at ages of 0–7, 51–58, 70–85 and 111 weeks were examined histologically
in this study. Mineralization and fibrosis were common findings in the aged rats, whereas they
were rarely found in the young ones; mineralization was present in 7, 44, 67 and 79% of males
and in 0, 32, 67 and 79% in females, and fibrosis was present in 0, 29, 48 and 44% of males and
0, 18, 40 and 35% of females at ages of 0–7, 51–58, 70–85 and 111 weeks, respectively. Striated
muscle fiber appeared regularly in the fibrosis region from 51–58 weeks of age when fibrosis
increased, while the origin of this fiber remained unclear. Vacuolation of pineal cells also
increased with age in both sexes, though the total incidence was low. There was a low incidence
of lymphocytic infiltration in both sexes, but this was not related to age. 相似文献
89.
Yamamoto Y Yamamoto T Yuto N Hildebrandt TB Lueders I Wibbelt G Shiina O Mouri Y Sugimura K Sakamoto S Kaewmanee S Nagaoka K Watanabe G Taya K 《The Journal of reproduction and development》2012,58(1):105-111
The objective of the present study was to define the secretion of prolactin (PRL) in pregnant African and Asian elephants. Levels of immunoreactive (ir-) PRL in serum and placental homogenates were measured by a heterologous radioimmunoassay (RIA) based on an ovine and human RIA system, and the localization of ir-PRL in the placenta was detected by immunohistochemistry using anti-human PRL. Circulating ir-PRL clearly showed a biphasic pattern during pregnancy in African and Asian elephants. Serum levels of ir-PRL started to increase from the 4 - 6th month of gestation and reached the first peak level around the 11-14th month. A second peak of circulating ir-PRL levels was observed around the 18-20th month of gestation followed by an abrupt decline after parturition. In contrast, in a case of abortion of an African elephant, the second peak of ir-PRL was not observed, and the levels remained low for about four months until parturition. The weight of the fetus delivered at the 17th month of gestation was 23.5 kg, which was quite small compared with normal fetuses in previous reports. Ir-PRL was detected in placental homogenates, and immunolocalization was observed in trophoblasts in both the African and Asian elephants, indicating that the placenta is the source of ir-PRL during pregnancy in elephants. The present results clearly demonstrated that circulating ir-PRL shows a biphasic pattern during normal pregnancy and that the placenta appears to be an important source of circulating ir-PRL during pregnancy in both African and Asian elephants. 相似文献
90.
Hiroshi KAJIKAWA Kei MIYAZAWA Ayako YANASE Yuki TANABE Yu TSUCHIDA Yukiko MITSUMOTO Yoshinobu KOZATO Makoto MITSUMORI 《Animal Science Journal》2012,83(4):299-304
Chemical composition and in situ degradation profiles were analyzed for 27 samples of dried distillers grains with solubles (DDGS) distributed in Japan, and a wide variation was found in neutral detergent fiber (NDF) content, which had positive relationships to detergent‐insoluble crude proteins such as neutral detergent‐insoluble crude protein (NDICP) and acid detergent‐insoluble CP (ADICP). Samples with lower NDF (< 35% on dry matter (DM)) showed higher soluble fractions of protein, but the degradation rate of microbially degradable protein in the rumen was not different in comparison with the samples with higher NDF, and no difference was shown between samples with higher and lower NDF after 24 and 48 h of in situ incubation for DM and CP degradation, respectively. The NDICP content in the digestion residue decreased with time of incubation, especially for samples with higher NDF, while the ADICP content increased. These results suggest that a part of the soluble fraction of CP in DDGS would be incorporated into NDICP by the heating process in bio‐ethanol production, which is still highly degradable, whereas another part of the fraction incorporated into ADICP would proceed to the advanced steps of irreversible amino‐carbonyl reaction. 相似文献