Development and validation of a questionnaire to evaluate the Quality of Life of cats with skin disease and their owners,and its use in 185 cats with skin disease          下载免费PDF全文

Chiara Noli  Stefano Borio  Antonella Varina  Carlo Schievano 《Veterinary dermatology》2016,27(4):247-e58

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Effect of a propolis extract and caffeic acid phenethyl ester on formation of aberrant crypt foci and tumors in the rat colon     

Borrelli F  Izzo AA  Di Carlo G  Maffia P  Russo A  Maiello FM  Capasso F  Mascolo N 《Fitoterapia》2002,73(Z1):S38-S43

We have studied the effect of propolis and its main active ingredient caffeic acid phenetyl ester (CAPE) on formation of aberrant crypt foci (ACF) and tumors in the rat colon in vivo. CAPE (50 mg/kg i.p.) reduced the formation of ACF and tumor induced by azoxymethane, while propolis ethanolic extract was without effect. These results suggest a potential anti-carcinogenesis of CAPE but not propolis.  相似文献   

Food deprivation stimulates the luteolytic capacity in the gilt     

Galeati G  Forni M  Govoni N  Spinaci M  Zannoni A  De Ambrogi M  Volpe S  Seren E  Tamanini C 《Domestic animal endocrinology》2007,33(3):281-293

The aims of this study were to study the effects of fasting on progesterone (P4) production in the pig and to verify whether fasting influences luteal expression of PGF(2alpha) receptor (FPr) and prostaglandin secretion. Superovulated prepubertal gilts were used; half of them were fasted for 72h starting on day 2 (F2) or 9 (F9) of the induced estrous cycle, respectively, while two groups (C2 and C9) served as respective controls. Plasma P4 and PGFM concentrations were determined by RIA while FPr mRNA expression in CLs collected at the end of fasting period was measured by real-time PCR. In experiment 1, plasma P4 concentrations in fasted gilts were significantly (P<0.01) higher than in controls starting from day 3 (F2; n=6) and 10 (F9; n=6). FPr mRNA expression was similar in F2 and C2 (n=6) CLs while it was significantly (P<0.05) higher in F9 than in C9 (n=6) CLs. In experiment 2, cloprostenol administered on day 12 significantly (P<0.05) increased FPr mRNA expression in CLs from both F9 (n=6) and C9 (n=6) gilts. At the time of cloprostenol injection PGFM levels were significantly higher (P<0.05) in the fasted group and cloprostenol-induced luteolysis in fasted but not in normally fed gilts. Results from this study indicate that fasting in prepubertal gilts induced to ovulate stimulates luteal P4 and PGFM production as well as FPr mRNA expression, thus increasing luteolytic susceptibility.  相似文献   

Development of a genomic tool for breed assignment by comparison of different classification models: Application to three local cattle breeds     

Hlne Wilmot  Jeanne Bormann  Hlne Soyeurt  Xavier Hubin  Gry Glorieux  Patrick Mayeres  Carlo Bertozzi  Nicolas Gengler 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2022,139(1):40-61

Assignment of individual cattle to a specific breed can often not rely on pedigree information. This is especially the case for local breeds for which the development of genomic assignment tools is required to allow individuals of unknown origin to be included to their herd books. A breed assignment model can be based on two specific stages: (a) the selection of breed-informative markers and (b) the assignment of individuals to a breed with a classification method. However, the performance of combination of methods used in these two stages has been rarely studied until now. In this study, the combination of 16 different SNP panels with four classification methods was developed on 562 reference genotypes from 12 cattle breeds. Based on their performances, best models were validated on three local breeds of interest. In cross-validation, 14 models had a global cross-validation accuracy higher than 90%, with a maximum of 98.22%. In validation, best models used 7,153 or 2,005 SNPs, based on a partial least squares-discriminant analysis (PLS-DA) and assigned individuals to breeds based on nearest shrunken centroids. The average validation sensitivity of the first two best models for the three local breeds of interest were 98.33% and 97.5%. Moreover, results reported in this study suggest that further studies should consider the PLS-DA method when selecting breed-informative SNPs.  相似文献   

Use of episcleral cyclosporine implants in dogs with keratoconjunctivitis sicca: pilot study          下载免费PDF全文

Laura Barachetti  Antonella Rampazzo  Carlo M. Mortellaro  Stefania Scevola  Brian C. Gilger 《Veterinary ophthalmology》2015,18(3):234-241

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Headspace sorptive extraction (HSSE), stir bar sorptive extraction (SBSE), and solid phase microextraction (SPME) applied to the analysis of roasted Arabica coffee and coffee brew.     

Carlo Bicchi  Cristina Iori  Patrizia Rubiolo  Pat Sandra 《Journal of agricultural and food chemistry》2002,50(3):449-459

Headspace sorptive extraction (HSSE) and stir bar sorptive extraction (SBSE), two recently introduced solventless enrichment techniques, have been applied to the analysis of the headspace of Arabica roasted coffee and of the headspace of the brew and of the brew itself. In both HSSE and SBSE enrichment is performed on a thick film of poly(dimethylsiloxane) (PDMS) coated onto a magnet incorporated in a glass jacket. Sampling is done by placing the PDMS stir bar in the headspace (gas phase extraction or HSSE) or by immersing it in the liquid (liquid phase extraction or SBSE). The stir bar is then thermally desorbed on-line with capillary GC-MS. The performance of HSSE and SBSE have been compared through the determination of the recoveries and relative abundances of 16 components of the coffee volatile fraction to classical static headspace (S-HS) and to headspace and in-sample solid phase microextraction (HS-SPME and IS-SPME, respectively) applying the fibers PDMS 100 microm, Carbowax/divinylbenzene 65 microm (CW/DVB), Carboxen/PDMS 75 microm(CAR/PDMS), polyacrylate 85 microm(PA), PDMS/divinylbenzene 65 microm(PDMS/DVB), and Carboxen/divinylbenzene/PDMS 50-30 microm(CAR/PDMS/DVB). In all cases, HSSE and SBSE gave higher recoveries, and this is entirely due to the high amount of PDMS applied.  相似文献   

Inhibition of Hepatocellular Carcinoma Growth by Ethyl Acetate Extracts of Apulian Brassica oleracea L. and Crithmum maritimum L.     

Gnocchi  Davide  Cesari  Gianluigi  Calabrese  Generosa Jenny  Capone  Roberto  Sabbà  Carlo  Mazzocca  Antonio 《Plant foods for human nutrition (Dordrecht, Netherlands)》2020,75(1):33-40

Plant Foods for Human Nutrition - Nowadays, a growing body of evidence supports the view that plants offer an extraordinary opportunity to discover and develop new promising therapeutic strategies...  相似文献   

Diving for science ‐ science for diving: volunteer scuba divers support science and conservation in the Mediterranean Sea          下载免费PDF全文

Carlo Cerrano  Martina Milanese  Massimo Ponti 《水产资源保护:海洋与淡水生态系统》2017,27(2):303-323

What's in an index? Comparing the ecological information provided by two indices to assess the status of coralligenous reefs in the NW Mediterranean Sea          下载免费PDF全文

Luigi Piazzi  Carlo Nike Bianchi  Enrico Cecchi  Giulia Gatti  Ivan Guala  Carla Morri  Stéphane Sartoretto  Fabrizio Serena  Monica Montefalcone 《水产资源保护:海洋与淡水生态系统》2017,27(6):1091-1100

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Growth and immune response of giant freshwater prawn Macrobrachium rosenbergii (De Man) postlarvae fed diets containing Chlorella vulgaris (Beijerinck)          下载免费PDF全文

Gian Carlo Maliwat  Stephanie Velasquez  Jan Lorie Robil  Merab Chan  Rex Ferdinand Traifalgar  Melchor Tayamen  Janice A Ragaza 《Aquaculture Research》2017,48(4):1666-1676

A 50‐day growth trial was conducted to evaluate the efficacy of Chlorella vulgaris (Beijerinck) as an ingredient in the diets of giant freshwater prawn Macrobrachium rosenbergii (De Man) postlarvae (PL30). Immune response (total haemocyte count and prophenoloxidase activity) was also assessed by subjecting postlarvae to a challenge test against Aeromonas hydrophila (Chester) for 14 days. Iso‐nitrogenous and iso‐lipidic test diets were prepared using a fishmeal‐based‐positive control diet (D0) and four basal diets with inclusion levels of 2% (D2), 4% (D4), 6% (D6) and 8% (D8) C. vulgaris. Postlarvae of M. rosenbergii were randomly stocked (mean initial body weight of 0.19 ± 0.02 g) in 30‐L tanks in three replicates per dietary treatment for evaluation of growth performance. Another set of postlarvae (mean initial body weight of 1.25 ± 0.02 g) was randomly distributed in 95‐L tanks in three replicates per dietary treatment for the assessment of immune response. Results showed that specific growth rate was significantly higher (P < 0.05) in postlarvae fed D4 and D6. Variations in values for carcass protein, lipid, moisture and ash were also evident. Postlarvae fed diets with Chlorella showed increased prophenol oxidase activity and total haemocyte counts. Moreover, survival rate after challenge with A. hydrophila was significantly increased (P < 0.05). Inclusion of C. vulgaris in diets enhanced immune response and resistance of M. rosenbergii postlarvae against A. hydrophila infection.  相似文献