Identification of candidate genes for paratuberculosis resistance in the native Italian Garfagnina goat breed     

Francesca Cecchi  Claudia Russo  Daniela Iamartino  Alessia Galiero  Barbara Turchi  Filippo Fratini  Sara Degl’Innocenti  Raffaele Mazza  Stefano Biffani  Giovanna Preziuso  Carlo Cantile 《Tropical animal health and production》2017,49(6):1135-1142

Paratuberculosis disease is a chronic bacterial disease infection of ruminants of global relevance, caused by MAP (Mycobacterium avium subsp. paratuberculosis). The present study was conducted on the Garfagnina goat breed that is an Italian native goat population registered on the Tuscan regional repertory of genetic resources at risk of extinction. Forty-eight adult goats (27 serologically positive to MAP-positive and 21 serologically negative to MAP-negative) belonging to a single flock that had experienced annual mortalities due to MAP infection were identified and genotyped with the Illumina GoatSNP60 BeadChip. Diagnosis was achieved by serological tests, as well as post-mortem examination of affected animals. A genome-wide scan was then performed on the individual marker genotypes, in an attempt to identify genomic regions associated with MAP infection disease. Nine significant markers were highlighted and they were located within, or nearby, annotated genes. Two genes found in this study encode are linked to protein kinases that are among the most important enzymes involved in the immune response to Johne’s disease, and four genes are involved in the functions of the Golgi complex.  相似文献   

Congenital cutaneous melanoma in a dog          下载免费PDF全文

Bruna Meyrer  Simone Passos Bianchi  Saulo Petinatti Pavarini  Daniel Guimarães Gerardi 《Veterinary dermatology》2017,28(2):228-e56

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Random pattern of parasitism and female-biased sex ratio in the egg parasitoid <Emphasis Type="Italic">Neochrysocharis formosa</Emphasis> attacking the pine sawfly <Emphasis Type="Italic">Diprion pini</Emphasis> in mountain forests of Spain     

Jesús Selfa  Carlo Polidori  Josep Daniel Asís  Luis De Pedro  Juli Pujade-Villar  José Tormos 《Phytoparasitica》2017,45(1):85-93

The most harmful hymenopteran pests of Pinus sylvestris L. are conifer sawflies from the family Diprionidae, including the widespread Diprion pini (L.). Natural enemies of this pest are still poorly known in many European areas where attacks occur. We studied the egg parasitoids of D. pini at four sites in two mountainous areas of Spain: the Sierra de Francia (western Spain) and the Sierra de Albarracín (eastern Spain). At all sites, the dominant egg parasitoid was Neochrysocharis formosa (Westwood) (Hymenoptera: Eulophidae), whereas other three chalcidoid species were rare. All these species were previously recorded in association with D. pini, but we report here their first record in Spain. Neochrysocharis formosa attacked up to 32.3% of egg clusters of D. pini in the Sierra de Albarracín and 18.5% in the Sierra de Francia. In the attacked egg clusters, this species parasitized up to 35% of eggs in the Sierra de Albarracín and 23.7% in the Sierra de Francia, with a marked female-biased sex ratio. Contrary to the clustered pattern of parasitism observed for N. formosa while attacking other gregarious diprionids, the oviposition in egg clusters of D. pini followed a random pattern, probably due to the froth roof (spumous coating) that covers its eggs and interferes with the egg-searching behavior of females. Indeed, other parasitoid species of D. pini have been reported to behave similarly.  相似文献   

Inhibition of Hepatocellular Carcinoma Growth by Ethyl Acetate Extracts of Apulian Brassica oleracea L. and Crithmum maritimum L.     

Gnocchi  Davide  Cesari  Gianluigi  Calabrese  Generosa Jenny  Capone  Roberto  Sabbà  Carlo  Mazzocca  Antonio 《Plant foods for human nutrition (Dordrecht, Netherlands)》2020,75(1):33-40

Plant Foods for Human Nutrition - Nowadays, a growing body of evidence supports the view that plants offer an extraordinary opportunity to discover and develop new promising therapeutic strategies...  相似文献   

Diving for science ‐ science for diving: volunteer scuba divers support science and conservation in the Mediterranean Sea          下载免费PDF全文

Carlo Cerrano  Martina Milanese  Massimo Ponti 《水产资源保护:海洋与淡水生态系统》2017,27(2):303-323

What's in an index? Comparing the ecological information provided by two indices to assess the status of coralligenous reefs in the NW Mediterranean Sea          下载免费PDF全文

Luigi Piazzi  Carlo Nike Bianchi  Enrico Cecchi  Giulia Gatti  Ivan Guala  Carla Morri  Stéphane Sartoretto  Fabrizio Serena  Monica Montefalcone 《水产资源保护:海洋与淡水生态系统》2017,27(6):1091-1100

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Growth and immune response of giant freshwater prawn Macrobrachium rosenbergii (De Man) postlarvae fed diets containing Chlorella vulgaris (Beijerinck)          下载免费PDF全文

Gian Carlo Maliwat  Stephanie Velasquez  Jan Lorie Robil  Merab Chan  Rex Ferdinand Traifalgar  Melchor Tayamen  Janice A Ragaza 《Aquaculture Research》2017,48(4):1666-1676

A 50‐day growth trial was conducted to evaluate the efficacy of Chlorella vulgaris (Beijerinck) as an ingredient in the diets of giant freshwater prawn Macrobrachium rosenbergii (De Man) postlarvae (PL30). Immune response (total haemocyte count and prophenoloxidase activity) was also assessed by subjecting postlarvae to a challenge test against Aeromonas hydrophila (Chester) for 14 days. Iso‐nitrogenous and iso‐lipidic test diets were prepared using a fishmeal‐based‐positive control diet (D0) and four basal diets with inclusion levels of 2% (D2), 4% (D4), 6% (D6) and 8% (D8) C. vulgaris. Postlarvae of M. rosenbergii were randomly stocked (mean initial body weight of 0.19 ± 0.02 g) in 30‐L tanks in three replicates per dietary treatment for evaluation of growth performance. Another set of postlarvae (mean initial body weight of 1.25 ± 0.02 g) was randomly distributed in 95‐L tanks in three replicates per dietary treatment for the assessment of immune response. Results showed that specific growth rate was significantly higher (P < 0.05) in postlarvae fed D4 and D6. Variations in values for carcass protein, lipid, moisture and ash were also evident. Postlarvae fed diets with Chlorella showed increased prophenol oxidase activity and total haemocyte counts. Moreover, survival rate after challenge with A. hydrophila was significantly increased (P < 0.05). Inclusion of C. vulgaris in diets enhanced immune response and resistance of M. rosenbergii postlarvae against A. hydrophila infection.  相似文献   

Simultaneous determination of ochratoxin A and cyclopiazonic,mycophenolic, and tenuazonic acids in cornflakes by solid-phase microextraction coupled to high-performance liquid chromatography     

Aresta A  Cioffi N  Palmisano F  Zambonin CG 《Journal of agricultural and food chemistry》2003,51(18):5232-5237

A solid-phase microextraction (SPME) method, coupled to liquid chromatography with diode array UV detection (LC-UV/DAD), for the simultaneous determination of cyclopiazonic acid, mycophenolic acid, tenuazonic acid, and ochratoxin A is described. Chromatographic separation was achieved on a propylamino-bonded silica gel stationary phase using acetonitrile/methanol/ammonium acetate buffer mixture (78:2:20, v/v/v) as mobile phase. SPME adsorption and desorption conditions were optimized using a silica fiber coated with a 60 microm thick polydimethylsiloxane/divinylbenzene film. Estimated limits of detection and limits of quantitation ranged from 3 to 12 ng/mL and from 7 to 29 ng/mL, respectively. The method has been applied to cornflake samples. Samples were subjected to a preliminary short sonication in MeOH/2% KHCO(3) (70:30, v/v); the mixture was evaporated to near dryness and reconstituted in 1.5 mL of 5 mM phosphate buffer (pH 3) for SPME followed by LC-UV/DAD. The overall procedure had recoveries (evaluated on samples spiked at 200 ng/g level) ranging from 74 +/- 4 to 103 +/- 9%. Samples naturally contaminated with cyclopiazonic and tenuazonic acids were found; estimated concentrations were 72 +/- 9 and 25 +/- 6 ng/g, respectively.  相似文献   

Soil solid phases effects on the proteomic analysis of Cupriavidus metallidurans CH34     

Laura Giagnoni  Francesca Magherini  Loretta Landi  Safiyh Taghavi  Daniel van der Lelie  Michele Puglia  Laura Bianchi  Luca Bini  Paolo Nannipieri  Giancarlo Renella  Alessandra Modesti 《Biology and Fertility of Soils》2012,48(4):425-433

Cupriavidus metallidurans CH34 is a completely sequenced soil-borne beta-proteobacterium with known genome and proteome. Comparative 2-D electrophoresis and protein mass spectrometry were used to compare the proteome of C. metallidurans CH34 from liquid culture and after incubation for 1, 3, and 12?days in microcosms containing quartz sand, kaolinite, montmorillonite, or an artificial soil. Results showed that proteome from liquid culture was similar to CH34 proteins extracted from sand and kaolinite, whereas the proteins extracted from artificial soil differed significantly and no proteins were detected from C. metallidurans CH34 incubated in the montmorillonite microcosms. Protein recovery decreased on prolonging incubation time in all microcosms. Mass spectrometry identification showed that the trend of lower recovery upon incubation time was independent on the putative function of protein. These results suggest that the soil solid phase influences the protein recovery and soil proteomic analysis and that distinction between protein recovery and protein expression in soil will be a challenging for soil proteomic researchers.  相似文献   

Effects of dietary soy isoflavones on estrogenic activity,cortisol level,health and growth in rainbow trout,Oncorhynchus mykiss          下载免费PDF全文

Maria Rita Pastore  Elena Negrato  Carlo Poltronieri  Giuseppe Barion  Maria Messina  Francesca Tulli  Cristina Ballarin  Lisa Maccatrozzo  Giuseppe Radaelli  Daniela Bertotto 《Aquaculture Research》2018,49(4):1469-1479

Soy isoflavones (the phytoestrogens genistein, daidzein and glycitein) may act as estrogen receptor agonists or antagonists. The aim of this study was to examine the effect of dietary isoflavones on growth, reproduction and health in rainbow trout (Oncorhynchus mykiss). Rainbow trout fed three experimental diets containing different concentrations of isoflavones (0, 500 and 1,500 ppm). Growth, estrogenic activity, plasma estradiol levels, gonadal development, state of stress and histological changes in selected tissues were evaluated at the end of 70 days. Neither growth performance nor the relative mRNA levels of Insulin Growth Factor I (igf‐I) in the liver were influenced by different levels of dietary isoflavones. Plasma and liver vitellogenin (VTG) protein levels and plasma 17‐β‐estradiol (E₂) were unaffected by treatments, although the correlation between plasma levels of E₂ and VTG densitometry values was significant (p < .05). The fish gonadosomatic index (GSI) did not significantly differ among the three experimental groups but correlated with plasma VTG densitometry values (p < .05). Plasma, muscle and fin cortisol concentrations fell within the normal welfare range and were not correlated with isoflavone levels. Histologically, the distal intestine showed a normal morphology with well‐differentiated enterocytes and in the liver hepatocytes were also normal. A supranuclear accumulation of lipid droplets in enterocytes and some lipid droplets in hepatocytes were observed in all tested groups, suggesting an impact of basal dietary lipid on transport/metabolism of fat in the fish. Overall, the present results suggest that the doses of isoflavones tested do not compromise rainbow trout reproduction, growth and health.  相似文献