Epidemiological study of Kabuto Mountain virus,a novel uukuvirus,in Japan     

Ngo T.B. TRAN  Hiroshi SHIMODA  Junko MIZUNO  Keita ISHIJIMA  Kenzo YONEMITSU  Shohei MINAMI  Supriyono  Yudai KURODA  Kango TATEMOTO  Milagros V. MENDOZA  Ai TAKANO  Masahiko MUTO  Haruhiko ISAWA  Kyoko SAWABE  Daisuke HAYASAKA  Ken MAEDA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(1):82

Kabuto Mountain virus (KAMV), the new member of the genus Uukuvirus, was isolated from the tick Haemaphysalis flava in 2018 in Japan. To date, there is no information on KAMV infection in human and animals. Therefore, serological surveillance of the infection among humans and wild mammals was conducted by virus-neutralization (VN) test and indirect immunofluorescence assay (IFA). Sera of 24 humans, 59 monkeys, 171 wild boars, 233 Sika deer, 7 bears, and 27 nutria in Yamaguchi Prefecture were analyzed by VN test. The positive ratio of humans, monkeys, wild boars, and Sika deer were 20.8%, 3.4%, 33.9% and 4.7%, respectively. No positive samples were detected in bears and nutria. The correlation coefficients between VN test and IFA in human, monkey, wild boar, and Sika deer sera were 0.5745, 0.7198, 0.9967 and 0.9525, respectively. In addition, KAMV was detected in one pool of Haemaphysalis formosensis ticks in Wakayama Prefecture. These results indicated that KAMV or KAMV-like virus is circulating among many wildlife and ticks, and that this virus incidentally infects humans.  相似文献   

Promotion of first internode elongation in perennial paddy weeds Sagittaria trifolia and Sagittaria pygmaea tubers by oxygen depletion and carbon dioxide          下载免费PDF全文

Daisuke Sasayama  Kenta Ikemachi  Hisateru Sakai  Hiroshi Fukayama  Kazuyuki Itoh  Tetsushi Azuma 《Weed Biology and Management》2016,16(4):147-156

The semiaquatic plants, S agittaria trifolia and S agittaria pygmaea, are perennial and troublesome weeds of rice paddy fields. These species mainly reproduce vegetatively via tubers, which sprout after the rice paddy fields are irrigated. Sprouting finally leads to the emergence of the shoots from the water in the paddy fields. In both species, the first internode elongates around the time of emergence and this lifts the shoot meristems in the soil, aiding in the establishment of the young plants. Therefore, elongation of the internode is a factor that determines the weed damage to rice caused by these species. In this study, the first internode elongation of both species was characterized. During emergence, S . trifolia and S . pygmaea showed distinct growth patterns. In S . trifolia, the internodes elongated before the emergence of the shoots from the water; whereas, in S . pygmaea, the internodes started to elongate only after emergence. We examined environmental prerequisites for internode elongation, and found that the internode elongation of S . trifolia was induced by submergence and was independent of the soil cover, while that of S . pygmaea required both submergence and covering. Next, we determined which gaseous factors were responsible for internode elongation. Treatment with ethylene, which enhances growth of several other aquatic and semiaquatic plants, did not stimulate internode elongation in either species under an anaerobic condition. Our results suggested that the gaseous factor stimulating internode elongation in S . trifolia and S . pygmaea may be oxygen depletion and carbon dioxide, respectively.  相似文献   

Suitability of Japanese codling as a raw material for surimi-based products revealed by primary sequence analysis of myosin heavy chain and thermal gel properties     

Watabe  Shugo  Ikeda  Daisuke  Mashiro  Takaki  Kagetakubo  Yuko  Takahashi  Yoshihiro  Uemura  Misaki  Mizusawa  Nanami  Koyama  Hiroki  Yasumoto  Ko  Jimbo  Mitsuru  Kan-no  Nobuhiro  Ueda  Tomohiro  Matsuoka  Yoko  Ueki  Nobuhiko  Wan  Jianrong 《Fisheries Science》2020,86(4):711-719

Fisheries Science - Complementary DNA encoding a part of myosin heavy chain was cloned from fast skeletal muscle of Japanese codling Physiculus japonicus. Japanese codling, a frequent catch off the...  相似文献   

Longline hook selectivity for red tilefish Branchiostegus japonicus in the East China Sea     

Hideyuki Yamashita  Daisuke Shiode  Tadashi Tokai 《Fisheries Science》2009,75(4):863-874

The catch of red tilefish by the Japanese longline fishery in the East China Sea has steeply declined during the last two decades, and resource management is clearly required. This study presents the size selectivity of hooks for red tilefish as a method to control first capture size, based on the results of longline fishing experiments using “Tainawa-bari” hooks of several sizes: Japanese nominal sizes J#10, J#11, J#12, J#13, J#14 and J#16. On the assumption of geometrical similarity in the combination of upper jaw length u and hook width g at a given retention probability, a single master curve s(R) = exp (−14.42 + 8.85R)/(1 + exp [−14.42 + 8.85R]) was estimated in terms of relative upper jaw length to hook width, R = u/g, using the SELECT method based on a multinomial distribution. The relative upper jaw length for 50% retention is 1.63, and the selection range is 0.25. Almost all fish over 300 mm in total length were retained by a hook of size J#12, but smaller fish with less commercial value were also caught. Moreover, J#10 hooks catch fish with total lengths of >250 mm efficiently. This indicates that J#10 and J#12 hooks that are currently used are too small to optimize yield per recruit and also for resource conservation.  相似文献   

Dietary arginine requirement of juvenile kuruma shrimp Marsupenaeus japonicus (Bate)     

Md. Shah Alam  Shin-ichi Teshima  Manabu Ishikawa  Daisuke Hasegawa  & Shunsuke Koshio 《Aquaculture Research》2004,35(9):842-849

The requirements for essential amino acids by kuruma shrimp, Marsupenaeus japonicus (Bate) have not yet been determined using the dose–response method due to poor utilization of crystalline amino acids (CAA). In a previous study, we produced coated CAA that could be used to retard leaching losses. Therefore, to determine the arginine requirement of juvenile kuruma shrimp, a growth study was conducted using test diets containing six levels of coated arginine (from 1.20% to 3.19% of diet) for 42 days. Crystalline amino acids was supplemented to correspond to the amino acid pattern in the whole body protein of the kuruma shrimp except for arginine. To reduce leaching losses, CAA were coated with carboxymethylcellulose (CMC) and diets were further bound with CMC and k‐carrageenan. Test diets were fed to triplicate groups of juveniles (0.25±0.02 g) twice a day. Proximate composition and the amino acid composition of shrimp whole bodies were analysed at the end of the feeding trial. Percent body weight gain (WG), specific growth rate, feed intake, feed efficiency ratio and protein retention efficiency were significantly affected by the dietary arginine concentrations. Estimating the arginine requirement of kuruma shrimp using broken‐line analysis of percent WG resulted in a value of 2.66% of diet or 5.32% of protein. After correcting for leaching of arginine from the diets, the optimum dietary value of arginine for kuruma shrimp was a little lower than the estimated value.  相似文献   

Sampling performance and operational quality of a frame trawl used to catch juvenile fish     

Kazuhiko Itaya  Yasuzumi Fujimori  Daisuke Shiode  Ichiro Aoki  Takashi Yonezawa  Susumu Shimizu  Teisuke Miura 《Fisheries Science》2001,67(3):436-443

SUMMARY: A sampling trawl with a rigid mouth; that is, the frame trawl, was designed so as to catch the large larval and juvenile fish more efficiently. The net consisted of four 13 m panels (nylon multifilament and twine diameter φ 0.75 mm, mesh size 8 mm). The frame of the net's mouth was composed of four stainless steel pipes (length 4 m, diameter φ 76 mm, thickness 5 mm). Several operations were conducted to investigate the capability of catching juveniles. A lot of Sardinops melanostictus juveniles, which are generally difficult to catch, were caught during every operation. Underwater observations proved that juveniles could not elude the capture. The frame trawl requires no special handling and is possible to operate in a rough sea.  相似文献   

Faster growth before metamorphosis leads to a higher risk of pseudoalbinism in juveniles of the starry flounder <Emphasis Type="Italic">Platichthys stellatus</Emphasis>, as suggested by otolith back-calculation     

Taizo Nishikawa  Masato Aritaki  Daisuke Shimizu  Toshihiro Wada  Masaru Tanaka  Masatomo Tagawa 《Fisheries Science》2010,76(5):827-831

The mechanism underlying malformation during flatfish metamorphosis is not clear. Here, the relationship between growth before metamorphosis and the occurrence of malformation after metamorphosis was examined in individuals reared in a single tank. Otoliths of starry flounder Platichthys stellatus larvae were labeled with alizarin complexone before metamorphosis [12 days after hatching (DAH)]. The positive linear regression between the lapillus otolith diameter and body length at 12 DAH enabled the estimation of individual larval size after completion of metamorphosis. At 12 DAH, the average body length of the typical pseudoalbino juveniles was significantly greater (5.37 mm) than that of the normal juveniles (5.13 mm) (P < 0.01, n = 100). In addition, of the larvae that were estimated to measure less than 4.8 mm at 12 DAH, approximately 80% metamorphosed into normal juveniles, whereas more than 60% of the larvae that measured more than 5.4 mm became pseudoalbino juveniles. These results indicate that the larvae that grow rapidly during the first 12 days are more likely to become pseudoalbino individuals after metamorphosis.  相似文献   

Egg collection from hatchery-reared broodstock of spotted halibut Verasper variegatus treated with LHRH analog     

Daisuke Shimizu  Yuichiro Fujinami  Sayumi Sawaguchi  Takahiro Matsubara 《Fisheries Science》2012,78(6):1245-1252

The objective of this study is to establish a method for acquiring large quantities of high-quality eggs for artificial fertilization from hatchery-reared broodstock of spotted halibut Verasper variegatus. We estimated the optimum conditions for implantation of luteinizing hormone-releasing hormone analog (LHRHa) cholesterol pellet (LHRHa-CP) to induce ovulation from two experiments which focused on the dose and the timing combined with monitoring of oocyte development by ovarian cannulation. From the results of cannulation, vitellogenesis of hatchery-reared broodstock was suggested to occur normally, although final oocyte maturation was not initiated in the first clutch of oocytes. In late December, oocytes in the most advanced clutch still underwent vitellogenesis, having diameter of about 0.82?mm. LHRHa-CP implantation during this period had no remarkable effects, except for administration at high dose (100???g/kg). In contrast, in mid January, when oocyte diameter reached about 0.95?mm, ovulation occurred in most individuals, even at low dose (20???g/kg). In mid February atretic oocytes became remarkable and LHRHa-CP implantation showed much lower performance in terms of egg quality. The diameter of growing oocytes converged to about 0.95?mm, which was that of fully grown postvitellogenic oocytes. Thus, oocyte diameter is suggested to be an effective indicator to estimate the timing of LHRHa-CP implantation.  相似文献   

Spray pollination as a labor-saving pollination system in Japanese pear (Pyrus pyrifolia (Burm.f.) Nakai): Development of the suspension medium     

Daisuke Sakamoto  Hiroko HayamaAkiko Ito  Yoshiki KashimuraTakaya Moriguchi  Yuri Nakamura 《Scientia Horticulturae》2009

Effects of suspension media used for spray pollination on pollen grain viability were investigated in Japanese pear (Pyrus pyrifolia (Burm.f.) Nakai ‘Kosui’). The suspension media tested in this study consisted of pectin methylesterase (PME) and polygalacturonase (PG) combined with either 0.1% agar or 0.04% xanthangum (XG). We also evaluated the influences of spray pollination on fruit set and fruit quality. Pollen grain viability, as measured by germination and pollen tube growth rates, was maintained in media supplemented with 0.1 mg L⁻¹ PME or 0.1 mg L⁻¹ PG. The level of fruit set after spray pollination using media containing PME or PG in combination with either 0.1% agar or 0.04% XG was almost the same as the level after artificial pollination by hand (hand pollination). The media containing XG combined with either PME or PG seemed to show better results for pollen grain viability and fruit set, although the results were variable from year to year. With regard to fruit size, shape and other parameters for fruit quality, spray pollination and hand pollination gave comparable results, irrespective of the medium composition. The time required for spray pollination was less than half of that required for hand pollination, and the amount of pollen grain required for spray pollination was one-third or less than the amount required for hand pollination. Thus, spray pollination may be a time- and labor-saving pollination system for the cultivation of Japanese pear.  相似文献   

Anatomic modifications in the enteric nervous system of JF1 mice with the classic piebald mutation     

Dang R  Sasaki N  Torigoe D  Agui T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(3):391-394

The Japanese Fancy Mouse 1 (JF1) has a characteristic coat color similar to a very old mutant, piebald. The mutation in JF1 and the classic piebald was previously thought to be the same recessive allele in the endothelin B receptor gene (Ednrb) according to the haplotype pattern, which is insufficient for this conclusion. In this study, we identified the same insertion of a retroposon-like element in intron 1 of the Ednrb gene in JF1 as in the classic piebald mutation by PCR. Further, we investigated whether the intestine shows neuronal intestinal malformations such as hypoganglionosis and immaturity of ganglion cells by histochemical staining. Though it has been assumed that the defect of neural crest-derived lineages is restricted to melanocytes in JF1, we found that the enteric innervation and neuronal density were impaired throughout the whole colon in JF1 mice.  相似文献