Extracts of Peels and Seeds of Five Varieties of Brazilian Jabuticaba Present High Capacity to Deactivate Reactive Species of Oxygen and Nitrogen     

Paludo  Michelly Cristiane  de Oliveira  Luciana Fontes  Hermosín-Gutiérrez  Isidro  Ballus  Cristiano Augusto  Ribeiro  Alessandra Braga  de Oliveira  Silvia Borges Pimentel  Godoy  Helena Teixeira 《Plant foods for human nutrition (Dordrecht, Netherlands)》2019,74(1):135-140

Plant Foods for Human Nutrition - Jabuticaba has a high concentration of phenolic compounds, which have a significant antioxidant capacity. Methodologies have been developed to evaluate the ability...  相似文献   

Relative effects of fragment size and connectivity on bird community in the Atlantic Rain Forest: Implications for conservation   总被引：1，自引：0，他引：1  

Alexandre C. Martensen  Rafael G. Pimentel  Jean Paul Metzger 《Biological conservation》2008,141(9):2184-2192

It is known that large fragment sizes and high connectivity levels are key components for maintaining species in fragments; however, their relative effects are poorly understood, especially in tropical areas. In order to test these effects, we built models for explaining understory birds occurrence in a fragmented Atlantic Rain Forest landscape with intermediate habitat cover (3%). Data from over 9000 mist-net hours from 17 fragments differing in size (2-175 ha) and connectivity (considering corridor linkages and distance to nearby fragments) were ranked under a model selection approach. A total 1293 individuals of 62 species were recorded. Species richness, abundance and compositional variation were mainly affected by connectivity indices that consider the capacity of species to use corridors and/or to cross short distances up to 30 m through the matrix. Bird functional groups were differently affected by area and connectivity: while terrestrial insectivores, omnivores and frugivores were affected by both area and connectivity, the other groups (understory insectivores, nectarivores, and others) were affected only by connectivity. In the studied landscape, well connected fragments can sustain an elevated number of species and individuals. Connectivity gives the opportunity for individuals to use multiple fragments, reducing the influence of fragment size. While preserving large fragments is a conservation target worldwide and should continue to be, our results indicated that connectivity between fragments can enhance the area functionally connected and is beneficial to all functional groups and therefore should be a conservation priority.  相似文献   

Phosphorus fractions and dynamics as affected by land-use changes in the Central Mexican highlands     

Jeannette S. Bayuelo-Jiménez  Takashi Muraoka  Eulogio de la Cruz-Torres  Esperanza Quintero-Ponce  Lydia C. Paredes-Gutiérrez  Mohammad Zaman 《Soil Use and Management》2020,36(2):240-249

Land-use change from forest to agriculture in the volcanic ash-derived soils of Mexico has increased over recent decades. It is likely that land uses and management practices, particularly fertilizer use have affected phosphorus (P) distribution and availability. The objective of this study was to evaluate the effects of land-use types (native forest and maize mono-cropping), and the related P addition, on the forms and distribution of soil P and their isotopic exchangeability. An Andisol, sampled from a cropping site, along with the contiguous area under native forest was treated with ³²P-labelled potassium phosphate (KH₂³²PO₄). The soil samples were extracted after incubation times of 7, 21, 35 and 49 days. Phosphorus content and ³²P recovery in fractions sequentially extracted were assessed for each incubation time. Total soil P was dominated by inorganic fractions (79 to 86%) in both land-use types. Resin-Pi, bicarbonate extractable inorganic P (Bic-Pi) and sodium hydroxide extractable inorganic P (NaOH_0.1-Pi) were all raised with P addition. However, the proportion of organic P fraction was reduced under cropped soil. The recovery of ³²P in soils with P addition indicates that resin-Pi, Bic-Pi and NaOH_0.1-Pi comprised nearly all the exchangeable P. In native soils with no P addition, more than 19% of the ³²P was recovered in Bic-Po and NaOH_0.1-Po forms. This finding indicates that organic P cycling is crucial when soil Pi reserves are presented in an inadequate amount. Ecologically based management has to be designed for replenishment and succeeding maintenance of soil organic P compounds to increase sustainable agricultural production.  相似文献   

Power and robustness of three whole genome association mapping approaches in selected populations     

Erbe M  Ytournel F  Pimentel EC  Sharifi AR  Simianer H 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2011,128(1):3-14

Selection is known to influence the linkage disequilibrium (LD) pattern in livestock populations. Spurious LD may lead to a higher number of false-positive signals in whole genome association mapping experiments. We compared three approaches for whole genome association mapping in a simulation study: single marker regression (SMR), a two-step approach, which analyses residuals corrected for family effects with an SMR (GRAMMAR), and a combined linkage and LD approach, which applies the quantitative transmission disequilibrium test to the Mendelian sampling term (MTDT). Three different scenarios were simulated: idealized random mating, limited number of parents and directional selection. The number of false-positive associations increased when the number of parents was limited. Mapping accuracy was the worst in the scenario with directional selection for all approaches. As SMR produced a high number of false-positive signals in small populations, results of whole genome scans in livestock analysed with SMR should be considered with caution. GRAMMAR was the most accurate approach, but also the least powerful one. The Bonferroni-corrected significance threshold seemed to be too stringent for this approach. Results obtained with MTDT changed only slightly with selected populations. MTDT combined sufficient power with a manageable number of false-positive associations in all scenarios.  相似文献   

High mortality and lesions of the central nervous system in trypanosomosis by Trypanosoma vivax in Brazilian hair sheep     

Galiza GJ  Garcia HA  Assis AC  Oliveira DM  Pimentel LA  Dantas AF  Simões SV  Teixeira MM  Riet-Correa F 《Veterinary parasitology》2011,182(2-4):359-363

Here, we report an outbreak of Trypanosoma vivax-induced trypanosomosis in Brazilian hair sheep on a farm in Paraíba state, a non-endemic region in northeastern Brazilian. Of 306 total sheep, 240 showed clinical signs and 216 died. Clinical signs included anorexia, lethargy, anemia, rough hair coat, weight loss, submandibular edema, abortion, and in some cases, neurological signs such as head pressing, lateral recumbence, paddling movements and muscle tremors. T. vivax was identified by blood smear analysis and polymerase chain reaction (PCR). At necropsy, animals exhibited watery blood, pale tissue coloring, and the presence of liquid in the peritoneal cavity and pericardial sac. Histologically, nonsuppurative myocarditis and meningoencephalitis with areas of malacia were observed. After treatment, no parasites were detected by blood smear analysis or PCR. Cattle and buffalo that remained in the same pasture were also infected but presented with asymptomatic infections. Epidemiological data suggest that T. vivax was introduced to the farm and the susceptible flock by buffalos that were asymptomatic carriers of the infection; T. vivax was most likely transmitted by Tabanus spp. bites and also iatrogenically.  相似文献   

Structural and ultrastructural characteristics of the tongue of the Collared Peccary (Pecari tajacu,Linnaeus, 1758)     

Gabriela Klein Barbosa  Carolina dos Santos Jacob  Jurandyr Pimentel Neto  Moacir Franco de Oliveira  Rose Eli Grassi Rici  Ii-Sei Watanabe  Adriano Polican Ciena 《Anatomia, histologia, embryologia》2020,49(4):532-540

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Testing the Accuracy of Vegetation-Based Ecoregions for Predicting the Species Composition of Blow Flies (Diptera: Calliphoridae)     

K Ketzaly Munguía-Ortega  Eulogio Lpez-Reyes  F Sara Ceccarelli 《Journal of insect science (Online)》2021,21(1)

To properly define ecoregions, specific criteria such as geology, climate, or species composition (e.g., the presence of endemic species) must be taken into account to understand distribution patterns and resolve ecological biogeography questions. Since the studies on insects in Baja California are scarce, and no fine-scale ecoregions based on the region’s entomofauna is available, this study was designed to test whether the ecoregions based on vegetation can be used for insects, such as Calliphoridae. Nine collecting sites distributed along five ecoregions were selected, between latitudes 29.6° and 32.0°N. In each site, three baited traps were used to collect blow flies from August 2017 to June 2019 during summer, winter, and spring. A total of 30,307 individuals of blow flies distributed in six genera and 13 species were collected. The most abundant species were Cochliomyia macellaria (Fabricius), Phormia regina (Meigen), and Chrysomya rufifacies (Macquart). The composition of the Calliphoridae community was different between the localities and three general groups have been distinguished, based on the species composition similarity (ANOSIM) results: Gulf-Desert, Mountains, and Pacific-Center. The vegetation-based ecoregions only reflect the blow fly species’ distributions to a certain extent, meaning that care must be taken when undertaking ecological biogeographical studies using regionalization based on organisms other than the focal taxa because vegetation does not always reflect fauna species composition.  相似文献   

First report and molecular characterization of Trypanosoma vivax in cattle from state of Pernambuco, Brazil     

Pimentel Dde S  Ramos CA  Ramos RA  de Araújo FR  Borba ML  Faustino MA  Alves LC 《Veterinary parasitology》2012,185(2-4):286-289

This paper describes an outbreak of Trypanosoma vivax for the first time in the state of Pernambuco, Brazil, affecting dairy cattle in the municipality of Itambé in the northern coastal zone of the state. Clinical signs compatible with infection by blood protozoa and epidemic miscarriages were observed. The diagnosis of T. vivax was confirmed through biometric microscopy and molecular analysis with PCR and DNA sequencing. The T. vivax isolate detected in the present study proved to be genetically very close to other Brazilian isolates of the protozoan despite being geographically distant.  相似文献   

Secretion of multi-protein migratory complex induced by Toxoplasma gondii infection in macrophages involves the uPA/uPAR activation system     

Schuindt SH  Oliveira BC  Pimentel PM  Resende TL  Retamal CA  DaMatta RA  Seipel D  Arnholdt AC 《Veterinary parasitology》2012,186(3-4):207-215

Toxoplasmosis is a world wide spread zoonosis caused by Toxoplasma gondii, an obligate intracellular parasite that is able to disseminate into deep tissues and cross biological barriers, reaching immunoprivileged sites such as the brain and retina. The parasite is able to infect macrophages and dendritic cells for dispersal throughout the body. However, the molecular mechanisms or outcomes of the subversion of the host cell are largely unknown. Recently our group established that metalloproteinases are involved in migration of infected macrophages. Herein, we evaluated the recruitment of host invasive machinery components in T. gondii infected murine macrophages. We showed by immunoprecipitation assays that MMP-9, CD44 TIMP-1 and uPAR were secreted as a multi-protein complex by infected macrophages. Zymographic analysis revealed that MMP-9 was present in its pro- and active form. Moreover, inhibition of uPA/uPAR pathway by PAI-1 decreased secretion of MMP-9 active forms, as well those associated to uPAR and TIMP-1, but not to CD44. Data presented here suggest that MMP-9 is secreted as a multiprotein complex by T. gondii infected macrophages, similar to that observed in metastatic cells. We further speculate that uPA/uPAR system is involved in the expression/secretion of complexes containing active MMP-9 forms.  相似文献   

Internal Transcribed Spacer Sequence-Based Phylogeny and Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Differentiation of Tilletia walkeri and T. indica     

Levy L  Castlebury LA  Carris LM  Meyer RJ  Pimentel G 《Phytopathology》2001,91(10):935-940

ABSTRACT A polymerase chain reaction-restriction fragment length polymorphism assay to distinguish Tilleita walkeri, a rye grass bunt fungus that occurs in the southeastern United States and Oregon, from T. indica, the Karnal bunt fungus, is described. The internal transcribed spacer (ITS) region of the ribosomal DNA repeat unit was amplified and sequenced for isolates of T. indica, T. walkeri, T. horrida, and a number of other taxa in the genus Tilletia. A unique restriction digest site in the ITS1 region of T. walkeri was identified that distinguishes it from the other taxa in the genus. Phylogenetic analysis of the taxa based on ITS sequence data revealed a close relationship between T. indica and T. walkeri, but more distant relationships between these two species and other morphologically similar taxa.  相似文献