Microbial HemG‐type protoporphyrinogen IX oxidase enzymes for biotechnology applications in plant herbicide tolerance traits     

Clayton T Larue  Joel E Ream  Xuefeng Zhou  Farhard Moshiri  Arlene Howe  Michael Goley  Oscar C Sparks  Steven T Voss  Erin Hall  Christine Ellis  Janice Weihe  Qungang Qi  Daniela Ribeiro  Xiaoping Wei  Shirley Guo  Artem G Evdokimov  Marguerite J Varagona  James K Roberts 《Pest management science》2020,76(3):1031-1038

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Uncertainty assessment of soya bean yield gaps using DSSAT‐CSM‐CROPGRO‐Soybean calibrated by cultivar maturity groups     

Wilson Wagner Ribeiro Teixeira  Rafael Battisti  Paulo Cesar Sentelhas  Milton Ferreira de Moraes  Adilson de Oliveira Junior 《Journal of Agronomy and Crop Science》2019,205(5):533-544

Soya bean yield gap can be caused by different factors resulting in uncertainties when the objective is to use such information for farm decision‐making and reference yield determination. Thus, this study aimed to quantify the soya bean yield gap for four sites, located in Southern and Midwestern Brazil, as well as the uncertainties of that related to cultivars, sowing dates, soil types and reference yields. The crop simulation model DSSAT‐CSM‐CROPGRO‐Soybean was calibrated for cultivars with similar maturity groups, based on the data obtained from the best farmers at the county level. The yield gap by water deficit (YG_WD) was obtained through the difference between potential and attainable yields, and that one caused by sub‐optimum crop management (YG_CM) by subtracting actual yield of each county, obtained from official statistics between 1989/90 and 2014/15 growing seasons, from the estimated attainable yield. The yield was simulated using four sowing dates, three soil types and two soya bean maturity groups by county. The reference yield uncertainty was quantified using yield reference from crop model and regional winners of the soya bean yield context, conducted by CESB (Brazilian Soybean Strategic Committee), for the growing seasons from 2013/14 to 2015/16. The crop model showed a good agreement between measured and simulated crop development and growth using calibration by maturity group, with low root mean square error (347 kg/ha). Southern sites had a mean YG_WD of 1,047 kg/ha, while in the Midwest, it was lower than 100 kg/ha. The YG_CM was 1,067, 528, 984 and 848 kg/ha, respectively, for Castro, PR, Mamborê, PR, Montividiu, GO and Primavera do Leste, MT, representing the opportunity for yield gain when having the best farmers as reference. The maturity groups, sowing dates and soil types showed to be an important source of uncertainty for yield gap determination, being recommended to investigate the farms in detail for an appropriate quantification. The reference yield showed expressive uncertainties, with some farmers presenting conditions to increase their soya bean yields by more than 3,000 kg/ha, when considering as reference the yields obtained by the winners’ farmers. These results show that uncertainties must be reduced when assessing farm yield gaps, in order to ensure that expected rate of soya bean yield growth could be reached by adopting the same technologies from CESB winners and best farmers in the county as a reference.  相似文献   

Using graph theory to analyse and assess changes in Mediterranean woodland connectivity     

Machado  Rui  Godinho  Sérgio  Guiomar  Nuno  Gil  Artur  Pirnat  Janez 《Landscape Ecology》2020,35(6):1291-1308

Landscape Ecology - The Portuguese montado is an agro-silvopastoral system, similar to the Spanish dehesa, known for its cultural, economic and ecological value. Despite its importance, contrasting...  相似文献   

Volatile emissions of watercress (Nasturtium officinale) leaves and passion fruit (Passiflora edulis) seeds against Meloidogyne incognita     

Marcela F Silva  Vicente P Campos  Aline F Barros  Willian C Terra  Mrcio P Pedroso  Vanessa A Gomes  Clerio R Ribeiro  Fabíola J Silva 《Pest management science》2020,76(4):1413-1421

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Tolerance to nymphs and adults of Mahanarva spectabilis (Hemiptera: Cercopidae) by forage plants in fertilized soils     

Roberta Alvarenga  Alexander Machado Auad  Jair Campos Moraes  Sandra Elisa Barbosa da Silva  Brunno Santos Rodrigues 《Pest management science》2019,75(8):2242-2250

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Acaricidal activity of Calea serrata (Asteraceae) on Boophilus microplus and Rhipicephalus sanguineus     

Ribeiro VL  Avancini C  Gonçalves K  Toigo E  von Poser G 《Veterinary parasitology》2008,151(2-4):351-354

Calea serrata (Asteraceae) is an endemic Southern Brazilian plant species used for religious and medicinal purposes. Previous study revealed the presence of chromenes, a class of natural compounds that possess insecticidal properties. This study reports the effect of the hexane extract from the aerial parts of this plant on egg hatchability, egg production and mortality rates of newly hatched larvae of the cattle tick Boophilus microplus. Larvae of Rhipicephalus sanguineus, the brown dog tick, received the same treatment. The extract was toxic to the eggs of B. microplus and to the larvae of both B. microplus and R. sanguineus.  相似文献   

Technical note: A novel technique to assess internal body fat of cattle by using real-time ultrasound     

Ribeiro FR  Tedeschi LO  Stouffer JR  Carstens GE 《Journal of animal science》2008,86(3):763-767

The objectives of this study were to describe a system to assess KPH fat by using real-time ultrasound (RTU) and to develop equations to predict total physical separable internal fat (IFAT) based on ultrasound measurements. Data for this study were obtained from 24 Angus steers fed either hay- or corn-based diets during the backgrounding phase. Steers were serially slaughtered in 3 groups: at weaning (baseline), then at 4 and 8 mo after weaning. A fourth group was composed of 4 steers from the hay-fed group that were slaughtered at approximately 10 mo after weaning. The RTU measurements were collected every 2 mo, with a preslaughter scan approximately 7 d before the slaughter time. The RTU measurements consisted of 12th- to 13th-rib backfat thickness, 12th to 13th ribeye area, percentage of intramuscular fat, and kidney fat depth, which was measured in a cross-sectional image collected between the first lumbar vertebra and the 13th rib. For kidney fat, the ultrasound probe was placed on the flank region approximately 15 cm from the midline of the animal. Images were stored in the ultrasound console, and measurements were taken between the ventral part of the iliocostalis muscle and the end of the KPH fat at the chute side. The relationship between carcass and ultrasound measurements in the depths of kidney fat (cKFd and uKFd, respectively) had an r(2) of 0.93, with a root mean square error (RMSE) of 1.14 cm. An allometric regression between carcass KPH weight (cKPHwt) and cKFd was identified, and the untransformed regression had an r(2) of 0.96. The linear regression between total IFAT and cKPHwt had an r(2) of 0.97, with an RMSE of 2.67 kg. Therefore, a system was developed to predict IFAT from uKFd measurements by combining these equations. Additionally, a single linear regression between IFAT and uKFd measurements was developed (r(2) = 0.89, RMSE = 5.32 kg). Even though the system of equations had a lower RMSE of prediction and greater r(2) compared with the single linear regression (4.80 vs. 5.10 kg and 0.91 vs. 0.89, respectively), there was no difference between these methods in predicting IFAT (P = 0.4936) by using a pairwise mean square error of prediction analysis. Our results indicated that uKFd measurements can accurately and precisely predict the cKFd of steers consuming either high concentrate or forage rations. The results also showed that cKFd is highly correlated with cKPHwt, which can be used to estimate total IFAT. More research is needed to further evaluate this technique with different feeding strategies, breeds, and sexes.  相似文献   

Cervical thymoma originating in ectopic thymic tissue in a cat     

Ana Lara‐Garcia  Maxey Wellman  Mary Jo Burkhard  Cecilia Machado‐Parrula  Victor E. Valli  Paul C. Stromberg  C. Guillermo Couto 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2008,37(4):397-402

Abstract: An 11‐year‐old female spayed domestic shorthair cat was referred to The Ohio State University Veterinary Teaching Hospital (OSU‐VTH) for evaluation of a 6 × 4 × 3.5 cm mass in the left midcervical region causing increased respiratory sounds and lateral deviation of the trachea. A fine needle aspirate of the mass was obtained before referral and the cytology results were compatible with a reactive lymph node. Immunocytochemistry showed increased numbers of CD3+ T lymphocytes and small numbers of CD20+ and CD79a+ medium to large lymphocytes. Differential diagnoses from the referral pathologist were T‐cell‐rich B‐cell lymphoma and feline Hodgkin's‐like lymphoma. A subsequent fine needle aspirate performed at the OSU‐VTH showed similar results. On flow cytometry the majority of cells were CD3+ T lymphocytes that were double positive for CD4 and CD8 (73%), compatible with either a double‐positive (CD4+CD8+) T‐cell lymphoma or lymphocytes from ectopic thymic tissue. The mass was surgically removed. Histopathology and immunohistochemistry of the mass revealed a predominant population of CD3+ small lymphocytes and small numbers of medium to large lymphocytes with moderate anisocytosis and anysokaryosis. A population of cytokeratin‐positive epithelial cells surrounded small microcystic structures filled with eosinophilic material and structures interpreted as Hassall's corpuscles. These findings were consistent with thymic tissue and a diagnosis of ectopic thymoma was made. PCR results for lymphocyte antigen receptor rearrangement (PARR) were negative. The cat had no evidence of disease 16 months after removal of the mass. To our knowledge this is the first report of an ectopic cervical thymoma in a cat. The clinical and diagnostic features of this unusual case will be useful in helping veterinarians and pathologists obtain a presurgical diagnosis and establish a prognosis for similar lesions.  相似文献   

Effects of intracameral injection of epinephrine and 2% lidocaine on pupil diameter,intraocular pressure,and cardiovascular parameters in healthy cats     

Tssia Moara Amorim  Nathalie Moro Bassil Dower  Matias Bassinello Stocco  Laurem Hellem Pizoni  Alexandre Pinto Ribeiro 《Veterinary ophthalmology》2019,22(3):276-283

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Acute phase proteins and biomarkers of oxidative status in feline spontaneous malignant mammary tumours     

Hugo Vilhena  Asta Tvarijonaviciute  Jos J. Cern  Ana C. Figueira  Snia Miranda  Ana Ribeiro  Ana Canadas  Patrícia Dias‐Pereira  Camila P. Rubio  Lorena Franco  Fernando Tecles  Ricardo Cabeas  Josep Pastor  Ana C. Silvestre‐Ferreira 《Veterinary and comparative oncology》2019,17(3):394-406

Acute phase proteins (APP) and biomarkers of oxidative status change in human and canine mammary tumours, however, they have not been studied in feline mammary tumours. The aims of this study were to investigate the APP and antioxidant responses in feline malignant mammary tumours, to evaluate their relation with tumour features, and to assess their prognostic value. Serum amyloid A (SAA), haptoglobin (Hp), albumin, butyrylcholinesterase (BChE), insulin‐like growth factor1 (IGF1), paraoxonase1 (PON1), total serum thiols (Thiol), glutathione peroxidase (GPox) and total antioxidant capacity determined by different assays, including trolox equivalent antioxidant capacity assessed by two different methodologies (TEAC1/2), ferric reducing ability of plasma (FRAP), and cupric reducing antioxidant capacity (CUPRAC), were determined in serum of 50 queens with spontaneous mammary carcinomas and of 12 healthy female cats. At diagnosis, diseased queens presented significantly higher SAA and Hp, and lower albumin, BChE, GPox, TEAC1, TEAC2 and CUPRAC than controls. Different tumour features influenced concentrations of APP and antioxidants. Increases in serum Hp, and decreases in albumin, Thiol and FRAP were significantly associated with neoplastic vascular emboli, metastasis in regional lymph nodes and/or in distant organs. Distant metastasis development during the course of the disease was associated with increases in SAA and TEAC1. At diagnosis, decreased albumin was associated with a longer survival, and BChE <1.15 μmoL/mL.minute was associated with a shorter survival time on multivariate analysis. Feline malignant mammary tumours are associated with an APP response and oxidative stress, and different tumour features influence the inflammatory response and the oxidative damage. Furthermore, some of these analytes proved to have prognostic value.  相似文献