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41.
Humic acid (HA) might benefit plant growth by improving nutrient uptake and hormonal effects. The effect of HA on growth, macro—and micronutrient contents, and postharvest life of gerbera (Gerbera jamesonii L.) cv. ‘Malibu’ were examined. Different levels of humic acid (0, 100, 500, and 1000 mg/L) were applied to nutrient solution.

Root growth increased at 1000 mg/L HA incorporated into the solution. Macro- and micronutrient contents of leaves and scapes including nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), iron (Fe), and zinc (Zn) were significantly enhanced by HA. However, high levels of HA decreased some nutrient contents.

Five-hundred mg/L HA increased the number of harvested flowers per plant (52%). Higher HA levels extended the vase life of harvested flowers by 2—3.66 days and could prevent and delay bent neck incidence. These postharvest responses were most probably due to Ca accumulation in scapes and hormone-like activity of HA.  相似文献   
42.
The plane tree is one of the most frequently used street trees in urban landscape in Iran and some Mediterranean countries and some age-old ones are found in these countries. Endophytes are microorganisms which associate with healthy plant tissues, persisting without causing diseases symptoms in the host and normally benefitting them through improving plant ability to withstand environmental stresses. In this contribution, the hypothesis of fungal endophytes existence in the plane trees as well as their relationship with morphological characteristics of trees and nutrient elements of leaves were studied in the central part of Iran. The experiment was performed according to an unbalanced completely randomized design. Approximate age and height of plane trees were recorded and then the greenness and visual appearance were evaluated. Healthy shoots and bark pieces were collected and surface sterilized then placed in petri dishes containing PDA. The results showed that endophytes frequency were significantly greater in age-old trees (60.04%) than young ones (39.96%). Endophytes existence had a positive correlation with iron (Fe) and potassium (K) concentrations of the leaves, and trees height and circumference. Also, there was a positive correlation between the presence of fungal endophytes and improved visual appearance of plane trees. The results suggest that fungal endophytes association enhanced nutrient elements assimilation in trees contributing at least partly to elevated survival of age-old plane trees.  相似文献   
43.
Identification of casual mutations in Hereditary Multiple Exostoses (HME) is important because of similar conditions in which multiple exostoses occur. Therefore mutation analysis can help to confirm the clinical diagnosis and to improve the management of therapy. HME is an inherited disorder of bone growth. HME can be referred to by various names such as Heredity Multiple Exostoses, Hereditary Multiple Osteochondromata, Multiple Carthaginous Exostoses, etc. People who have HME grow exostoses, or bony bumps, on their bones which can vary in size, location and number depending on the individual. HME is inherited in an autosomal dominant manner with an estimated prevalence of 1/50,000 in western countries. At least three loci (EXT1, EXT2 and EXT3) thought to be involved in this skeletal disease. Approximately 90% of affected families possess mutations in the coding regions of EXT1 and EXT2 genes and the majority of these mutations cause loss of function. EXT1 and EXT2 genes encode related members of a putative tumor suppressor family. In this first report from Iran we identified a frame shift mutation (1100-1101 insA) in exon 3 of EXT1 gene in a family being suspicious of HME. This mutation leads to a premature stop codon and previously not described. Additionally, we have found an unreported silent mutation in the exon six of EXT1 gene with uncertain significance.  相似文献   
44.
To evaluate the effects of different level of Na salinity (0, 3, 6 and 9 dS m(-1)) on growth, yield and yield component of Kabuli (Hashem and Jam) and Desi (Kaka and Pirooz) chickpea cultivars a factorial experiment based on randomized complete block design with four replications was carried out in Research Greenhouse of Mokrian Agricultural Extension Center near Mahabad, Iran at 2006. Seeds of four chickpea cultivars were grown under 0, 3, 6 and 9 dS m(-1) levels of salinity until maturity. Salinity reduced the plant growth, flower, pod and seed number and seed weight. As increase in salinity, the undesirable effect of Na+ was more pronounced and reached the highest value at 9 dS m(-1) in all cultivars. Four chickpea cultivar have different responses to salinity and the Kabuli cultivars seemed to have a greater capacity for salt tolerance compared to Desi cultivars. Hahshem cultivar has the highest salinity tolerance among all cultivars.  相似文献   
45.
The objective of this study was to evaluate the effect of different forms and doses of rosemary on chemical, microbial, and sensory properties of rainbow trout fed nine different diets: control (C), 20 g.kg?1 rosemary powder (20RP), 40 g.kg?1 rosemary powder (40RP), 0.3 g.kg?1 rosemary extract (0.3RE), 0.6 g.kg?1 rosemary extract (0.6RE), 0.15 g.kg?1 rosemary nanopowder (0.15RNP), 0.3 g.kg?1 rosemary nanopowder (0.3RNP), 0.15 g.kg?1 butylated hydroxyanisole (BHA) (0.15BHA), and 0.3 g.kg?1 BHA (0.3BHA). After 8 weeks’ feeding, the fish fillets were sampled on the 1st, 4th, 8th, 12th, and 16th days and then stored on 4°C. Lower value of pH, peroxide value (PV), total volatile base nitrogen (TVB-N), free fatty acids (FFA), and thiobarbituric acid (TBA) were reported in fish fed with RP, RE, RNP, and BHA; among them, RNP groups had the lowest properties (p < 0.05). Furthermore, lower total viable aerobic bacterial counts (TVC) and psychotropic counts (PTC) were observed in the fillets of the fish fed with rosemary treatments, especially in RNP treatments (7.52–9.41 log10 CFU.g?1), along with a slower spoilage in terms of sensory factors (texture, color, odor, and overall) on the 16th day. Finally, use of natural antioxidant nanorosemary in the diet may positively affect fish fillet quality and delay post-mortem deterioration.  相似文献   
46.
Background:Premature ovarian failure is a heterogeneous disorder, leading to early menopause. Several genes have been identified as the cause of non-syndromic POF. Our aim was to explore the genetic defects in Iranian patients with POF. Methods:We studied a family with three females exhibiting non-syndromic POF. WES was performed for one of the affected individuals after ruling out the presence of CGG repeat expansion at FMR1 gene in the family. Sanger sequencing was used to confirm the candidate sequence variants in the proband, and screening of the detected mutation was performed for the other affected and unaffected members of the family. Results:A homozygous frameshift mutation, c.349delC, was identified in FCN3 gene in the proband and two other patients. The parents and two healthy brothers were heterozygous for the mutation, and an unaffected sister was homozygous for wild type. Conclusion:This is the first report of a mutation in FCN3 gene in a family with POF. Our findings can lead to the enhancement of genetic databases of patients with POF, specifically for families with high-risk background. Key Words: Ficolin-3, Premature ovarian failure, Whole exome sequencing  相似文献   
47.
The properties of soils, even in short distances differ from point to point. These spatial variabilities may be described by geostatistics. This method determines the spatial structure of a specified variable. In this study, we measured the sorptivity of soil in field under tilled and no-tilled conditions with different sampling distances by using a simple method and performed the geostatistical analysis on the obtained data. The measurements were done on the corners of a regular network having dimensions of 5 m × 5 m in a tilled and no-tilled soil, and 0.5 m × 0.5 m in the same tilled soil. The results revealed that sorptivity had spatial structure in a tilled soil with 0.5 m × 0.5 m grid distances and no spatial structure occured in 5 m × 5 m distances, so it was believed that sorptivity is a structural variable only for short distance. The range of variogram was between 3.09 and 3.42 m for the sorptivity with 0.5 m × 0.5 m grids. The spherical model was the best-fitted theoretical variogram. In addition to the simple method for sorptivity measurement, we measured the sorptivity using double rings, which is known as Talsma method. Statistical tests showed a significant and non-significant difference between the two methods in 5 m × 5 m and 0.5 m × 0.5 m grids, respectively. In the simple method for sorptivity measurement, a ring with 10 cm height, 10 cm diameter, and about 2 cm depth of water was applied, but the rings used in the Talsma method were about three to four times larger. Therefore, it is concluded that for short distances, the sorptivity of simple method is in accordance with that obtained in Talsma method. The kriging method was used to estimate the presumed unknown values of sorptivity in some locations in the 0.5 m × 0.5 m grid distances. Results revealed that kriging method is capable enough to estimate the sorptivity if the variable has a spatial structure.  相似文献   
48.
The oak decline is known as one of the most destructive complex diseases causing high economic losses around the world, especially in Iran. The main objective of the present study was to investigate the possible role of bacteria as causative agents of oak decline in the Zagros forests of Iran. To do this, stem, root and leaf samples were taken from symptomatic Persian oak trees (Quercus brantii) in different zones of Zagros forests (Ilam Province, Iran). From 150 bacterial isolates, 20 showed pathogenicity against Geranium seedlings. Among 20 hypersensitivity test positive strains, four strains showed pathogenicity against oak saplings. Based on morphological and 16S rRNA gene sequence analysis, three strains were identified as Bacillus pumilus and one strain as non‐sporulating Gram‐negative Stenotrophomonas maltophilia. Pathogenicity studies of different B. pumilus and S. maltophilia strains revealed that they have potential to cause the disease in oak saplings and symptoms of disorder in Persian oak trees. To our knowledge, there are no previous records of B. pumilus and S. maltophilia causing decline on Fagaceous trees like Q. brantii. More detailed field and molecular studies are required to confirm the absolute role of such bacteria in occurrence of oak decline in Zagros forests.  相似文献   
49.
In this study, the efficiency of a novel droplet vitrification technique along with different doses of fish antifreeze protein (AFP) type III on Persian sturgeon thawed spermatozoa quality (motility duration and motility percentage) was investigated. Semen of seven male individuals was pooled in equal volumes and diluted with 4°C Tris‐Hcl (100 mM), pH = 8 extenders containing 0, 5, 10, 15 μM of AFP type III in a ratio of 1:1 (semen/extenders). Treated semen was dropped into liquid nitrogen. Solidified droplets were stored for 2, 60 and 120 days and thawed by plunging them into a tube containing 5 mL Tris‐Hcl (100 mM), pH=8 with 1% BSA at 37°C. Motility duration in all treatments had no significant difference comparing to fresh sperm (P > 0.05), but their motility percentage was significantly lower. Treatment with 10 μM of AFP had significantly higher motility percentage (16.11 ± 0.5%) comparing to other treatments (P < 0.05). There was no significant difference between 0, 5, 15 μM of antifreeze protein treatments (P > 0.05), suggesting that antifreeze protein effectiveness are highly dose dependent, and dose of 10 μM is appropriate in Persian sturgeon spermatozoa droplet vitrification. Besides, the present technique obtained higher quality of spermatozoa comparing to its analogue techniques.  相似文献   
50.
The possible role of chitinase in in vitro growth inhibition of the wheat pathogens Fusarium graminearum and Bipolaris sorokiniana by Bacillus pumilus SG2 was investigated. B. pumilus SG2, a chitinolytic bacterium producing two different chitinases, was previously isolated from the saline deserts of Iran. When grown in Spizizen salts medium with colloidal chitin, B. pumilus SG2 secreted two chitinases into the medium, resulting in growth inhibition of F. graminearum and abortion of hyphal elongation of B. sorokiniana. In contrast, when glucose was used as the carbon source, the chitinases were not expressed and antifungal activity of the B. pumilus SG2 was completely abolished. These results confirmed that expression of the B. pumilus SG2 chitinases is under the control of two types of regulation, special regulation by chitin and global regulation by glucose. We demonstrated that chitinases are the main components that caused hyphal inhibition activity of B. pumilus SG2. Hyphal inhibition of F. graminearum and B. sorokiniana was stable in agar for a minimum of 14 days.  相似文献   
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