The onset of stress response in rainbow trout Oncorhynchus mykiss embryos subjected to density and handling     

Gholamreza Ghaedi  Bahram Falahatkar  Vahid Yavari  Mohammad T. Sheibani  Gholamreza Nikbakht Broujeni 《Fish physiology and biochemistry》2015,41(2):485-493

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Effect of Dietary Gamma‐irradiated and Fermented Soybean Meal on the Growth Performance,Body Composition,and Digestive Enzymes Activity of Caspian Brown Trout,Salmo trutta caspius,Juvenile          下载免费PDF全文

Ebrahim Sotoudeh  Jamshid Amiri Moghaddam  Gholamreza Shahhosseini  Mohammad Sadegh Aramli 《Journal of the World Aquaculture Society》2016,47(6):830-842

This experiment was conducted to evaluate the feasibility of gamma‐irradiated soybean meal (SBM) as a dietary ingredient on the performance, body composition, and digestive enzyme activity of Caspian brown trout, Salmo trutta caspius, juveniles. Five isonitrogenous and isoenergetic diets were formulated, in which fish meal was replaced by untreated (untreated SBM), gamma‐ray irradiated at 15 (15ISBM) and 30 (30ISBM) kGy, fermented (FSBM), fermented 15 KGy irradiated soybean meal (FISBM), respectively. Juvenile fish (2.1 ± 0.3 g) were fed with the test diets for 6 wk. Final body weight was significantly affected by dietary SBM treatment with highest values in fish fed FISBM and FSBM (P < 0.05). There were no significant differences in hepatosomatic and visceral indexes, condition factor, and survival among experimental groups (P > 0.05). The carcass proximate composition of lipid and protein in Caspian brown trout in FSBM and FISBM groups was higher than that of other groups. Levels of most whole‐body amino acids were not significantly different among Caspian brown trout fed the five diets. Results of this experiment showed that replacing SBM with FSBM and FISBM in the diet significantly increased the activities of proteolytic enzymes activities (P < 0.05). However, no significant differences were observed in both amylase and lipase activities among dietary treatments. Results of this study indicated that the level of gamma radiation used did not work but fermentation of SBM did increase some growth parameters of Caspian brown trout.  相似文献   

Effects of Water Hardness and Calcium: Magnesium Ratios on Reproductive Performance and Offspring Quality of Macrobrachium rosenbergii          下载免费PDF全文

Kamran Rezaei Tavabe  Gholamreza Rafiee  Mohammad Mehdi Shoeiry  Shadab Houshmandi  Michael Frinsko  Harry Daniels 《Journal of the World Aquaculture Society》2015,46(5):519-530

This study evaluated the effect of specific calcium and magnesium ratios at two hardness values on reproductive performance and offspring quality of Macrobrachium rosenbergii brood‐females. Hatchery water containing 110.5 mg/L CaCO3 hardness was considered baseline as it has been used successfully to hatch and rear larvae and was used as the control treatment. At each hardness value of 150 and 190 mg/L CaCO₃, four experimental water treatments were made in triplicate. Each treatment was adjusted using soluble salts (CaCl₂·H₂O, MgSO₄·7H₂O, and MgCl₂·6H₂O) to provide the hardness and calcium to magnesium ratios of 0:20, 20:80, 50:50, and 80:20 needed. Each of the experimental tanks (140 L) were stocked with six females and one male. The results indicated that both hardness and Ca:Mg ratios affect brood‐female reproductive qualitative parameters such as intermolt period, egg hatchability, egg dry weight, and egg‐clutch somatic index (ESI) parameters (P < 0.05) but not fecundity and eggs per spawn. The results revealed that brood‐females at 150 mg/L hardness showed greater reproductive performance than at 190 and 110.5 mg/L hardness. The findings also demonstrated that the treatment 50Ca50Mg at 150 mg/L hardness with 38.8 mg/L calcium and 12.9 mg/L magnesium had optimum reproductive performance and offspring quality for M. rosenbergii brood‐females.  相似文献   

Assessment of vitamin C different levels in aquaponic system on Nile tilapia (Oreochromis niloticus) and saffron (Crocus sativus): growth performances,hematology, serum biochemistry,and digestive enzyme activity     

Javanmardi  Sina  Rezaei Tavabe  Kamran  Rafiee  Gholamreza  Moradi  Saeed  Sivaji  Divya 《Aquaculture International》2022,30(4):2145-2163

The aim of this study was to investigate the possibility of improving the growth and physiological indices of plant and fish by adding different levels of ascorbic acid (vitamin C) to water in the aquaponic system using Nile tilapia (Oreochromis niloticus) and saffron plant (Crocus sativus). 240# fish (12.5?±?0.21 gr) and 120# saffron corms (2.8?±?0.12 gr) were randomly assigned to 15 experimental units and underwent treatments of adding 0 (control), 2, 4, 6, and 8 mg L^?1 ascorbic acid to water, every 6 days, for 8 weeks. The fish final weight and subsequently other growth performance indices increased in the treatment of 4 mg L^?1 vitamin C compared to other treatments (P?≤?0.05). Some growth performances of saffron plants such as saffron production, in treatment of 6 mg L^?1, were significantly higher than the control group and reached from 17.34?±?0.27 mg flower^?1 in the control group to 25.4?±?1.61 mg flower^?1 in treatment of 6 mg L^?1. Measuring the serum biochemical parameters of the fish showed that, in the treatment of 8 mg/L ascorbic acid, the cortisol content in the blood reached its maximum (21.49?±?2.42 µg dL^?1). The trypsin activity in proximal intestine and mid-intestine significantly increased in treatments of 2, 4, and 6 mg L^?1, respectively. The current experiment showed that, by adding 4–6 mg/L ascorbic acid to the aquaponic system water (every 6 days), the optimal levels of Nile tilapia and saffron plant growth performances would occur.

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Immune responses to oral and IM administration of M2e-Hsp70 construct     

Farzaneh Assadian  Gholamreza Nikbakht  Siavash Niazi  Reza Farahani Khaltabadi  Monireh Jahantigh 《Veterinary research communications》2014,38(2):157-163

The eukaryotic expression plasmid of M2e-Hsp70 is a candidate M2e-based DNA vaccine. In order to evaluate the immunization potential of this construct, Specific Pathogen Free chickens were immunized either intramuscularly or orally. Mutant Salmonella typhimurium was used as carrier for oral delivery of the M2e-Hsp70 construct. M2e-specific humoral and cellular responses were tested by ELISA and Lymphocyte Proliferation Assay, respectively. Our results indicate that both humoral and cellular immune responses are conferred against M2e-Hsp70 plasmid in either of the intramuscular or oral routes of administration; however, these responses are significantly higher in intramuscular injection in contrast to oral administration. When it comes to mass vaccination of commercial chicken flocks oral administration is preferred due to the ease of application as well as its capability of eliciting mucosal, humoral and cellular immune responses; so measurements should be taken to improve the immunization potency of our orally delivered DNA vaccine.  相似文献   

Molecular detection of Theileria spp. in sheep and vector ticks in the North Khorasan Province, Iran     

Ahmad Rashidi  Gholamreza Razmi 《Tropical animal health and production》2012,45(1):299-303

A survey was carried in North Khorasan Province, Iran in 2010–2011, designed to identify Theileria spp. infections of both sheep and ticks. The tick species were also examined. Ninety sheep from different flocks were clinically examined, and blood samples and ixodid ticks were collected. Light microscopy of blood smears revealed Theileria spp. infection in 37 (41.1 %), while 74 (82.2 %) of blood samples were positive using semi-nested PCR. Theileria ovis, Theileria lestoquardi, and mixed infection were detected in 63/90 (70 %), 5/90 (5.5 %), and 6/90 (6.6 %) of samples, respectively. Of the 434 ticks that were collected, the most prevalent species was Rhipicephalus turanicus (69.3 %) followed by Hyalomma marginatum turanicum (18.4 %), Dermacentor marginatus (6.4 %), and Rhipicephalus bursa (5.7 %). The ticks were separated into 42 tick pools, and the salivary glands were dissected out in 0.85 % (w/v) saline under a stereomicroscope and examined using semi-nested PCR. Three pools of H. marginatum turanicum salivary glands were infected with T. ovis and T. lestoquardi, and one pool of R. turanicus was infected with T. ovis. Based on these results, it is concluded that the prevalence of T. ovis is higher than T. lestoquardi and that H. marginatum turanicum and R. turanicus are likely vectors of T. lestoquardi and T. ovis in this area.  相似文献   

Allelic polymorphism in the second exon of Ovar-DRB1 in fat-tailed sheep     

Nikbakht G  Rezaii H  Stear MJ  Talebi MA  Mahmoudzadeh H 《Veterinary journal (London, England : 1997)》2012,192(3):547-549

Ovar-DRB1 is one of the most important response genes in the major histocompatibility complex (MHC) class II region of sheep. Gene polymorphism in the second exon of Ovar-DRB1 in three different Iranian fat-tailed sheep breeds (Lori-Bakhtiari, Shaul and Zandi) was analyzed by either restriction fragment length polymorphisms (RFLP) or direct sequencing. A total of 92 Lori-Bakhtiari, 40 Shaul, and 47 Zandi sheep were examined. PCR-RFLP identified 17 genotypes in Lori-Bakhtiari sheep, 12 in Shaul sheep and 11 in Zandi sheep. Collectively, 24 different genotypes could be found for Iranian fat-tailed sheep. Using direct sequencing, seven new sequences in exon 2 of the Ovar-DRB1 gene were identified. Generalized linear modeling with a multinomial error structure showed that the sheep populations had significantly different allele frequencies.  相似文献   

Synthesis and characterization of nanocrystalline CoWO4@silk fibers with antibacterial activity under ultrasound irradiation     

Azadeh Azadbakht  Amir Reza Abbasi  Nourolah Noori  Ezzat Rafiee  Mojtaba Taran 《Fibers and Polymers》2013,14(4):687-692

The present investigation reports the novel synthesis of CoWO₄ nanoparticles@silk fiber under ultrasound irradiation. The effect of temperature, power of ultrasound irradiation and sequential dipping steps in growth of the CoWO₄ particles were studied. Results show a decrease in the particles size as the temperature and power of irradiation decreased. The fibers containing CoWO₄ nanoparticles were tested for their antibacterial efficacy against E. coli and S. aureus and were found to possess significant antibacterial activity. The results show the CoWO₄ nanoparticles@silk with strongest fluorescence characteristics can be obtained in this method. The physicochemical properties of the nanoparticles were determined by X-ray diffraction (XRD), scanning electron microscopy (SEM) and fluorescence spectroscopy.  相似文献   

Land use change modeling through an integrated Multi-Layer Perceptron Neural Network and Markov Chain analysis(case study: Arasbaran region,Iran)     

Vahid Nasiri  Ali.A.Darvishsefat  Reza Rafiee  Anoushirvan Shirvany  Mohammad Avatefi Hemat 《林业研究》2019,(3):943-957

Temporal land use/land cover (LULC) change information provides a variety of applications for informed management of land resources. The aim of this study was to detect and predict LULC changes in the Arasbaran region using an integrated Multi-Layer Perceptron Neural Network and Markov Chain analysis. At the first step, multi-temporal Landsat images (1990, 2002 and 2014) were processed using ancillary data and were classified into seven LULC categories of high density forest, low-density forest, agriculture, grassland, barren land, water and urban area. Next, LULC changes were detected for three time profiles, 1990–2002, 2002–2014 and 1990–2014. A 2014 LULC map of the study area was further simulated (for model performance evaluation) applying 1990 and 2002 map layers. In addition, a collection of spatial variables was also used for modeling LULC change processes as driving forces. The actual and simulated 2014 LULC change maps were cross-tabulated and compared to ensure model simulation success and the results indicated an overall accuracy and kappa coefficient of 97.79% and 0.992, respectively. Having the model properly validated, LULC change was predicted up to the year 2025. The results demonstrated that 992 and 1592 ha of high and lowdensity forests were degraded during 1990–2014,respectively, while 422 ha were added to the extent of residential areas with a growth rate of 17.58 ha per year. The developed model predicted a considerable degradation trend for the forest categories through 2025, accounting for 489 and 531 ha of loss for high and low-density forests, respectively. By way of contrast, residential area and farmland categories will increase up to 211 and 427 ha, respectively. The integrated prediction model and customary area data can be used for practical management efforts by simulating vegetation dynamics and future LULC change trajectories.  相似文献   

Effects of the prebiotic mannan‐oligosaccharide on feed deprived zebrafish: Growth and reproduction          下载免费PDF全文

Mohammad Navid Forsatkar  Mohammad Ali Nematollahi  Gholamreza Rafiee  Hamid Farahmand  Christian Lawrence 《Aquaculture Research》2018,49(8):2822-2832

This study was designed to investigate whether the prebiotic mannan‐oligosaccharides (MOS) can reduce the deleterious impacts of feed deprivation on growth and reproduction in zebrafish. In the growth performance experiment, juvenile fish were distributed in the following four treatments: normal‐control (NC), starved‐control (SC), normal‐prebiotic (NP) and starved‐prebiotic (SP). After 8 weeks, NP and SC fish showed the highest and lowest growth patterns, respectively among treatments. Standard length, specific growth rate and feed conversion ratio did not differ significantly between NC and SP treatments. Autochthonous lactic acid bacteria (LAB) were significantly higher in NP and SP than the NC and SP treatments. Feed restriction resulted in significantly lower concentrations of thyroxine. In the reproductive performance experiment, additional juveniles were fed as in the growth experiment. Crossing in the final week of the experiment resulted in successful spawning in only NP fish, which showed mature sperm and oocytes in histological examinations. The number of spermatozoa was significantly lower in the fish that experienced feed restriction; however, the oocytes of SP females were at the same maturation level to that observed in NC females. Sex‐steroids changed after both starvation and MOS supplementation where NC and SP showed no differences in the levels of testosterone and female's 17β‐estradiol. These results indicate that MOS supplemented diet reduced some side effects of feed deprivation (final weight and length, SGR, FCR and levels of LAB and T3), and suggest that supplementation of the diet with MOS may ameliorate some of the negative effects of feed deprivation in zebrafish.  相似文献