The effect of hypervitaminosis D3 on the bones of the hen. 1. Light microscopy studies     

H Krempl  H Bacowsky 《Tier?rztliche Praxis》1988,16(2):153-158

46 one day old chickens, divided into five groups, were fed an alternately composed vitamin D3 ration during 36 days. In the group depleted of vitamin D3 the typical rickets were found. When treated with a standard ration (2000 I.U. vit. D3/kg food) after 15 days depletion of vitamin D3 the chickens showed control-group parameters within 3 weeks. Using 60,000 I.U. vitamin D3 after a period of 15 days depletion evoked rather an abrupt beginning of healing tendency accompanied by signs of intoxication which were enhanced in those chickens treated with 120,000 I.U. vitamin D3: increase in number of osteoblasts, calcification of osteoblastic mitochondrial membranes and endothelial cell membranes, as well as degeneration of osteoblasts together with a strong reaction of eosinophils. There did not occur necrosis of osteocytes or of bone structure. Erythrocytes, being embedded in ground substance without endothelial surrounding, was a common finding in every group.  相似文献   

Genotypic Variability in Biomass Protein and Nucleic Acids in Four Linum Species to Sodicity Stress     

A. K. Singh  B. B. Singh 《Journal of Agronomy and Crop Science》1991,167(1):1-7

Differences in gas exchange parameters i.e. carbon exchange rate (CER), transpiration (Tr), water vapor conductance (g) were investigated, under a controlled environment, in a semi-dwarf (SD) Triticum aestivum line, its tall (TL) near isoline and the Payne (PA) variety characterized by small leaves. The plants were maintained in: (a) optimal substrate moisture condition (CTR); (b) salinized by watering with a 0.12 m NaCl solution (SLT); (c) water stressed by withholding waterings during a period of six days (STR); (d) stressed and salinized during the same period and with the same saline solution (S + S). CER and Tr were negatively affected by the stresses; SLT and S + S treatments had a higher WUE compared to their respective controls CTR and STR.
SD line had a better performance in terms of CER and WUE, particularly when water and salt stresses interacted. Leaf anatomy and assimilation/internal CO₂ concentration curves contributed to explaining the SD performance.  相似文献   

Staining pattern of the brush border and detection of cytoplasmic granules in the uriniferous tubules of female DBA/2Cr mouse kidney: comparison among various fixations and stains.     

A Yabuki  S Suzuki  M Matsumot  M Kurohmaru  Y Hayashi  H Nishinakagawa 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(12):1339-1342

The proximal straight tubules of the female mouse kidney exhibit heavy periodic acid Schiff (PAS) staining in their brush borders and numerous cytoplasmic granules. In the present study, the female DBA/2Cr mouse kidney was examined, using various fixatives (formalin, PFA, PLP, Zamboni's, Bouin, or Carnoy solution) and various staining methods (HE, PAS, alcian blue, periodic acid methenamine-silver (PAM), toluidine blue, azan, or Congo red). Under azan and PAM, the staining pattern of the brush border was similar to that of PAS, and few effects of the fixative were observed. Cytoplasmic granules were clearly detected with PAM as well as PAS. However, these granules were not detected with Carnoy solution. Furthermore, distribution of granules differed between PAS and PAM.  相似文献   

Editorial     

C. Guillermo Couto 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1994,8(2):71-71

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Detection of antibodies to bovid herpesvirus 2 infection of cattle in Syria     

D Tabbaa  H Liebermann 《Archiv fuer experimentelle veterinaermedizin》1989,43(6):929-931

Neutralising antibody to bovid herpesvirus 2 was demonstrated in the serum of 31 (10.8%) of 286 heads of cattle in north, south, and west Syria. 38% of titres were 1:2 to 1:8. There is no published report on isolation of this virus in Syria.  相似文献   

Antibody response to crustacean ectoparasites in rainbow trout, Oncorhynchus mykiss (Walbaum), immunized with Argulus foliaceus L. antigen extract     

N. RUANE  T. K. MCCARTHY  P. REILLY 《Journal of fish diseases》1995,18(6):529-537

Abstract. A humoral antibody response was demonstrated by ELISA in rainbow trout immunized intraperitoneally with extracts from the branchiuran ectoparasite Argulus foliaceus. A similar immunization protocol produced a higher titre response in a rabbit. Both trout and rabbit identified several antigenic components on immunoblots. ELISA and immunoblotting indicated that rainbow trout and rabbit anti-A. foliaceus sera identified components from the parasitic copepods Lepeophtheirus salmonis and Caligus elongatus.  相似文献   

Yield response to insecticide treatment of a perennial ryegrass sward following sheep grazing or cutting by machine     

R.O. CLEMENTS  L.F. HENDERSON† 《Grass and Forage Science》1994,49(1):105-106

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Effects of dietary molybdenum on nematode and host during Haemonchus contortus infection in lambs.     

N F Suttle  D P Knox  K W Angus  F Jackson  R L Coop 《Research in veterinary science》1992,52(2):230-235

The addition of molybdenum (0.05 mmol kg-1 dry-matter) to the diet of lambs given a trickle infection of Haemonchus contortus larvae (500 third stage larvae d-1 over six weeks) reduced mean faecal egg counts (epg) from 3952 to 2312 +/- 402 by 32 days (P less than 0.02) and greatly reduced the mean number of worms recovered from the abomasum 14 days after infection ceased (907 compared with 4167: P less than 0.01). Infection reduced haemoglobin concentrations less in lambs given molybdenum although the difference was small relative to the reduction in worm burden. Lambs not given molybdenum had low intraepithelial mast cell counts in the abomasal mucosa and less abomasal hypertrophy than expected from abomasal parasitism. Molybdenum did not consistently reduce the copper status of the host or the parasite. Previous exposure to molybdenum greatly reduced protein but not proteinase activity in, or secreted by, adult worms cultured for eight hours. It is suggested that molybdenum either increased the inflammatory response which preceded worm rejection or that it indirectly enhanced that reaction by reducing the effectiveness of copper-dependent, anti-inflammatory enzymes in the gastrointestinal mucosa.  相似文献   

Serological differentiation of five bluetongue virus serotypes in indirect ELISA.     

D H Du Plessis 《The Onderstepoort journal of veterinary research》1992,59(2):119-122

The serological reactivity in indirect ELISA of five different bluetongue virus (BTV) serotypes (4, 10, 15, 16 & 20) was compared using polyclonal antisera raised against virus particles and an outer structural protein, VP2. Rabbit and sheep antisera against BTV-10 produced higher ELISA values with their homologous antigens than with heterologous serotypes. A hyperimmune rabbit serum specific for virus particles was able to distinguish heterologous serotypes from each other, but a sheep serum from an infected animal was not. An antiserum directed against VP2, the protein responsible for serotype specificity in neutralization tests, was not serotype-specific in ELISA and cross-reacted with other serotypes. The discriminatory ability of a BTV-4 antiserum was improved by cross-absorption with heterologous antigens. This greatly reduced the ELISA signals with heterologous serotypes and produced an antiserum that was effectively serotype-specific.  相似文献   

Temporal ELISA response of rams to Brucella ovis following experimental infection or vaccination   总被引：1，自引：0，他引：1  

J C Chin  J W Plant 《Research in veterinary science》1989,46(1):73-78

Sera from rams vaccinated with antigens extracted chaotropically from Brucella ovis by potassium thiocyanate treatment were used to optimise a whole-cell, enzyme-linked immunosorbent assay (CELISA) and to monitor the temporal serological response of rams which had been challenged with infected semen by the intranasal or intrapreputial route. Three patterns of CELISA response were detected. Thirteen of 15 rams intranasally challenged did not respond serologically (pattern 1 or nil response). Only one of 15 rams in the intranasal group exhibited a rise and fall response with CELISA (pattern 2), while another showed a rise and surge response (pattern 3). The numbers of rams in the intrapreputial group which displayed a pattern 1 or 2 or 3 response were four, nine and two, respectively. No ram with a pattern 2 response excreted B ovis in the semen or showed any other evidence of infection, whereas rams with a pattern 3 response excreted B ovis in the semen and developed palpable lesions. Intrapreputially challenged rams that were CELISA-positive consistently mounted an antibody response against B ovis about two to four weeks earlier than intranasally challenged rams.  相似文献