Effects of Co‐culture of Cumulus Oocyte Complexes with Denuded Oocytes During In Vitro Maturation on the Developmental Competence of Cloned Bovine Embryos          下载免费PDF全文

A‐N Ha  M Fakruzzaman  K‐L Lee  J‐I Bang  G‐K Deb  Z Wang  I‐K Kong 《Reproduction in domestic animals》2015,50(2):292-298

This study evaluated the effects of co‐culture of immature cumulus oocyte complexes (COCs) with denuded immature oocytes (DO) during in vitro maturation on the developmental competence and quality of cloned bovine embryos. We demonstrated that developmental competence, judged by the blastocyst formation rate, was significantly higher in the co‐cultured somatic cell nuclear transfer (SCNT+DO, 37.1 ± 1.1%) group than that in the non‐co‐cultured somatic cell nuclear transfer (SCNT‐DO, 25.1 ± 0.9%) group and was very similar to that in the control IVF (IVF, 38.8 ± 2.8%) group. Moreover, the total cell number per blastocyst in the SCNT+DO group (101.7 ± 6.2) was higher than that in the SCNT‐DO group (81.7 ± 4.3), while still less than that in the IVF group (133.3 ± 6.0). Furthermore, our data showed that mRNA levels of the methylation‐related genes DNMT1 and DNMT3a in the SCNT+DO group were similar to that in the IVF group, while they were significantly higher in the SCNT‐DO group. Similarly, while the mRNA levels of the deacetylation‐related genes HDAC2 and HDAC3 were significantly higher in the SCNT‐DO group, they were comparable between the IVF and SCNT+DO groups. However, the mRNA levels of HDAC1 and DNMT3B were significantly higher in the SCNT+DO group than in the other groups. In conclusion, the present study demonstrated that co‐culture of COCs with DO improves the in vitro developmental competence and quality of cloned embryos, as evidenced by increased total cell number.  相似文献   

Intrapancreatic ectopic splenic tissue found in a cloned miniature pig     

Ok Jae Koo  Seung-Kwon Ha  Sol Ji Park  Hee Jung Park  Su Jin Kim  Daekee Kwon  Jung Taek Kang  Joon Ho Moon  Eun Jung Park  Goo Jang  Byeong Chun Lee 《Journal of veterinary science (Suw?n-si, Korea)》2015,16(2):241-244

Somatic cell nuclear transfer (SCNT) is a cost-effective technique for producing transgenic pigs. However, abnormalities in the cloned pigs might prevent use these animals for clinical applications or disease modeling. In the present study, we generated several cloned pigs. One of the pigs was found to have intrapancreatic ectopic splenic tissue during histopathology analysis although this animal was grossly normal and genetically identical to the other cloned pigs. Ectopic splenic tissue in the pancreas is very rare, especially in animals. To the best of our knowledge, this is the first such report for cloned pigs.  相似文献   

国槐SSR-PCR反应体系的建立与优化     

张明静  庞彩红  李际红  李双云  付茵茵  哈新英  夏阳 《山东农业科学》2022,54(5):10-15

利用单因素试验结合L₁₆（4⁵）正交试验设计对国槐SSR-PCR反应体系中的5个主要影响因素(Mg²⁺、引物、dNTPs、ExTaq酶、模板DNA用量)进行优化,确定最佳反应体系为（10μL）:25 mmol/L Mg²⁺1.0μL,2.5 mmol/L dNTPs 0.2μL,10μmol/L上下游引物共0.5μL,5 U/μL ExTaq酶0.05μL,30 ng/μL模板DNA 1.0μL,10×PCR Buffer 1.0μL,ddH₂O 6.25μL。随机挑选4对SSR引物和8个国槐种质对试验确定的最佳反应体系进行验证,均能获得清晰、稳定的条带。该优化反应体系的建立为利用SSR标记对国槐种质资源进行深入研究和利用奠定了基础。  相似文献   

桃新品种——“北农早艳”、“北农二号”     

潘季淑  哈贵增  孟新法 《中国农业大学学报》1980,(2):75-75

培育过程:我们根据1956—57年北京郊区桃品种的调查情况,自1958年开始桃的育种。经过1958、60—63年的杂交工作,定植了一批杂种实生苗。先后从中初选出十多个优良单系。这些单系有的在北京郊区试种,有的引入上海、江苏、山西、四川等地试种。十多年来,其中二个单系已有小片生产果园或已扩大试种。1979年又经过较全面的鉴定,分别定名为北农早艳(原名6—25)与北农二号(原代号60—23—2)。  相似文献   

Control of synaptic strength by glial TNFalpha     

Beattie EC  Stellwagen D  Morishita W  Bresnahan JC  Ha BK  Von Zastrow M  Beattie MS  Malenka RC 《Science (New York, N.Y.)》2002,295(5563):2282-2285

Activity-dependent modulation of synaptic efficacy in the brain contributes to neural circuit development and experience-dependent plasticity. Although glia are affected by activity and ensheathe synapses, their influence on synaptic strength has largely been ignored. Here, we show that a protein produced by glia, tumor necrosis factor alpha (TNFalpha), enhances synaptic efficacy by increasing surface expression of AMPA receptors. Preventing the actions of endogenous TNFalpha has the opposite effects. Thus, the continual presence of TNFalpha is required for preservation of synaptic strength at excitatory synapses. Through its effects on AMPA receptor trafficking, TNFalpha may play roles in synaptic plasticity and modulating responses to neural injury.  相似文献   

Preparation of high molecular weight atactic poly(vinyl alcohol) by photo-induced bulk polymerization of vinyl acetate     

Won Seok Lyoo  Wan Shik Ha 《Fibers and Polymers》2001,2(2):108-115

Vinyl acetate was polymerized in ultraviolet-ray initiated bulk system at low temperatures using 2,2′-azobis(2,4-dimethylvaleronitrile) (ADMVN) or 2,2′-azobis(isobutyronitrile) (AIBN) as the photoinitiator, respectively. High molecular weight (HMW) poly(vinyl alcohol) (PVA) having number-average degree of polymerization (P _n ) of 3,900–7,800 and syndiotactic diad (S-diad) content of 52.5–54.0% could be prepared by complete saponification of synthesized linear poly(vinyl acetate) (PVAc) havingP _n of 5,900–9,400 obtained at conversion of below 30%.P _n of PVA using ADMVN was larger than that of PVA using AIBN. On the other hand, conversion of the former was smaller than that of the latter, and it was found that the initiation rate of the ADMVN was lower than that of AIBN. This could be explained by a fact that the rate of photolysis of AIBN is faster than that of ADMVN due to the higher quantum yield or dissociation rate constant of AIBN than that of ADMVN. TheP _n , syndiotacticity, and whiteness of PVA from PVAc polymerized at lower temperatures were superior to those of PVA from PVAc polymerized at higher temperatures.  相似文献   

Analysis of genetic diversity in Prunus sibirica L. in inner Mongolia using SCoT molecular markers     

Buer  Ha  Rula  Sa  Wang  Zi Yuan  Fang  Shu  Bai  Yu´e 《Genetic Resources and Crop Evolution》2022,69(3):1057-1068

Genetic Resources and Crop Evolution - Population genetic diversity contributes to the protection and utilization of germplasm resources, especially via genetic breeding. In the present study,...  相似文献   

Analysis of genetic variability and agronomic performance of Indian lettuce (Lactuca indica L.)     

Oliya  Bal Kumari  Kim  Moon Young  Ha  Jungmin  Lee  Suk-Ha 《Genetic Resources and Crop Evolution》2022,69(3):1313-1327

Genetic Resources and Crop Evolution - Lactuca indica L. is an undomesticated medicinal crop in the Asteraceae family. The study was carried out to identify elite genotypes for lettuce cultivation...  相似文献   

Motility and fertility of boar semen after liquid preservation at 5°C for more than 2 weeks     

Zhao Namula  Yoko Sato  Risa Kodama  Kouta Morinaga  Vien Viet Luu  Masayasu Taniguchi  Michiko Nakai  Fuminori Tanihara  Kazuhiro Kikuchi  Takashi Nagai  Takeshige Otoi 《Animal Science Journal》2013,84(8):600-606

This study investigated the effects of skim milk on the quality and fertility of boar spermatozoa under long‐term chilled preservation. Semen samples were stored in Modena solution supplemented with 0 (control) to 50 mg/mL skim milk at 5°C for 4 weeks; spermatozoa stored with 7.5 and 15 mg/mL of skim milk (7.5‐SM and 15‐SM groups, respectively) exhibited significantly higher motility indices than those of the control group up to 3 weeks (P < 0.05), and the 7.5‐SM group showed improved motility indices even after 4 weeks (P < 0.05). In vitro fertilization using spermatozoa in the 7.5‐SM and 15‐SM groups stored at 5°C for 2 weeks showed significantly higher fertilization rates of spermatozoa and the development rates to blastocyst than the control group (P < 0.05), and the 7.5‐SM group showed similar rates of fertilization and blastocyst formation in the fresh non‐stored spermatozoa group. After artificial insemination using spermatozoa stored for 2 weeks in the 7.5‐SM group, healthy piglets were obtained. Boar spermatozoa can be stored at 5°C in a Modena solution containing skim milk. Supplementation of 7.5 mg/mL skim milk improves boar spermatozoa motility and fertility even after liquid preservation at 5°C for 2 weeks.  相似文献   

黑河上游冰沟流域4种土壤有机碳分布特征与土壤特性的关系     

秦嘉海  张勇  赵芸晨  王治江  高海宁  赵静 《上海交通大学学报(农业科学版)》2013,(5):200-206

为了探讨黑河上游冰沟流域不同土壤有机碳分布特征与土壤特性的关系,为黑河上游冰沟流域水源涵养研究提供科学依据,采用野外采样,室内分析方法,研究了4种土壤有机碳分布特征及其与土壤特性的关系。结果表明：4种土壤有机碳含量和密度在整个土壤剖面上均表现为：森林灰褐土>高山灌丛草甸土>高山草甸土>山地栗钙土,且垂直分布均随土壤深度增加而减少,说明黑河上游冰沟流域的森林灰褐土比其它土壤更有利于土壤有机碳储存和积累。森林灰褐土0～10 cm土壤有机碳密度为4.54 kg·m^-2,略高于我国森林土壤0～10 cm土壤平均碳密度（4.24 kg·m^-2）,说明黑河上游冰沟流域的森林灰褐土区雨量充沛,林下植被丰富,凋落物现存量充足。4种土壤0～10 cm土层有机碳含量是整个土壤剖面土壤有机碳含量的30.69%～37.99%,有机碳密度是整个土壤剖面有机碳密度的29.31%～36.77%,说明黑河上游冰沟流域土壤有机碳含量和有机碳密度在表层具有很强的表聚性,不合理的人为活动引发的水土流失极易造成土壤有机碳储量的减少,应增加黑河上游冰沟流域植被覆盖度,保护生态环境,减少水土流失。4种土壤有机碳、全氮、CEC、田间持水量、团聚体在整个土壤剖面上均随土层深度增加而降低,而土壤容重、pH值在整个土壤剖面上均随土层深度增加而增大。经回归统计分析,4种土壤有机碳含量与土壤田间持水量、团聚体、全氮、CEC之间呈显著的正相关关系,与土壤容重、pH之间呈显著的负相关关系。这种变化规律与多数学者研究结果基本一致。  相似文献